MX2009010840A - Metodo de seleccion para internalizar acidos nucleicos. - Google Patents
Metodo de seleccion para internalizar acidos nucleicos.Info
- Publication number
- MX2009010840A MX2009010840A MX2009010840A MX2009010840A MX2009010840A MX 2009010840 A MX2009010840 A MX 2009010840A MX 2009010840 A MX2009010840 A MX 2009010840A MX 2009010840 A MX2009010840 A MX 2009010840A MX 2009010840 A MX2009010840 A MX 2009010840A
- Authority
- MX
- Mexico
- Prior art keywords
- nucleic acids
- selection method
- cell internalizing
- cells
- internalizing nucleic
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1048—SELEX
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
La presente invención incluye composiciones y métodos para poner en contacto una o más células con una genoteca aleatoria que contiene ARN, tratar las células puestas en contacto con un agente desnaturalizante o digestión con una o más nucleasas, y extraer de las células uno o más ácidos nucleicos internalizados resistentes a las nucleasas o desnaturalizantes.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US91079207P | 2007-04-09 | 2007-04-09 | |
US98465607P | 2007-11-01 | 2007-11-01 | |
PCT/US2008/059805 WO2008124798A1 (en) | 2007-04-09 | 2008-04-09 | Selection method for cell internalizing nucleic acids |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2009010840A true MX2009010840A (es) | 2009-11-05 |
Family
ID=39831424
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2009010840A MX2009010840A (es) | 2007-04-09 | 2008-04-09 | Metodo de seleccion para internalizar acidos nucleicos. |
Country Status (12)
Country | Link |
---|---|
US (1) | US8298764B2 (es) |
EP (1) | EP2140034B1 (es) |
JP (1) | JP5368425B2 (es) |
KR (1) | KR20100015558A (es) |
CN (1) | CN101680037B (es) |
AU (1) | AU2008237057A1 (es) |
BR (1) | BRPI0810828A2 (es) |
CA (1) | CA2683602A1 (es) |
ES (1) | ES2394384T3 (es) |
IL (1) | IL201315A (es) |
MX (1) | MX2009010840A (es) |
WO (1) | WO2008124798A1 (es) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011142798A2 (en) * | 2010-05-13 | 2011-11-17 | Albert Einstein College Of Medicine Of Yeshiva University | Methods of preparing targeted aptamer prodrugs |
US20130150251A1 (en) * | 2011-10-28 | 2013-06-13 | The Curators Of The University Of Missouri | Methods for analyzing lariat rna |
KR101352430B1 (ko) * | 2011-12-02 | 2014-01-20 | 건국대학교 산학협력단 | Ha1에 특이적으로 결합하는 rna 앱타머 및 조류인플루엔자 바이러스에 대한 응용 |
US20130203057A1 (en) * | 2012-01-20 | 2013-08-08 | Biohelix Corporation | Step-wise detection of multiple target sequences in isothermal nucleic acid amplification reactions |
KR102052150B1 (ko) | 2015-03-31 | 2019-12-05 | 아사히 가세이 가부시키가이샤 | 폴리이미드 필름, 폴리이미드 바니시, 폴리이미드 필름을 이용한 제품 및 적층체 |
JPWO2017047750A1 (ja) * | 2015-09-18 | 2018-07-05 | 公立大学法人大阪市立大学 | 細胞内移行性dna及びそれを用いた目的分子の細胞内導入方法 |
WO2019044736A1 (ja) * | 2017-08-28 | 2019-03-07 | 静岡県公立大学法人 | コリバクチンおよびコリバクチン産生菌の検出方法および検出プローブ |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7767803B2 (en) * | 2002-06-18 | 2010-08-03 | Archemix Corp. | Stabilized aptamers to PSMA and their use as prostate cancer therapeutics |
US20050256071A1 (en) * | 2003-07-15 | 2005-11-17 | California Institute Of Technology | Inhibitor nucleic acids |
WO2005082894A1 (en) * | 2004-02-27 | 2005-09-09 | Peter Maccallum Cancer Institute | Cell targeting conjugates |
US20060105975A1 (en) * | 2004-04-19 | 2006-05-18 | Shannon Pendergrast | Aptamer-mediated intracellular delivery of therapeutic oligonucleotides |
WO2006096754A2 (en) | 2005-03-07 | 2006-09-14 | Archemix Corp. | Stabilized aptamers to psma and their use as prostate cancer therapeutics |
-
2008
- 2008-04-09 EP EP08745416A patent/EP2140034B1/en not_active Not-in-force
- 2008-04-09 JP JP2010503189A patent/JP5368425B2/ja not_active Expired - Fee Related
- 2008-04-09 US US12/100,390 patent/US8298764B2/en not_active Expired - Fee Related
- 2008-04-09 CN CN200880019111XA patent/CN101680037B/zh not_active Expired - Fee Related
- 2008-04-09 MX MX2009010840A patent/MX2009010840A/es active IP Right Grant
- 2008-04-09 WO PCT/US2008/059805 patent/WO2008124798A1/en active Application Filing
- 2008-04-09 AU AU2008237057A patent/AU2008237057A1/en not_active Abandoned
- 2008-04-09 KR KR1020097021407A patent/KR20100015558A/ko not_active Application Discontinuation
- 2008-04-09 ES ES08745416T patent/ES2394384T3/es active Active
- 2008-04-09 CA CA002683602A patent/CA2683602A1/en not_active Abandoned
- 2008-04-09 BR BRPI0810828-5A2A patent/BRPI0810828A2/pt not_active IP Right Cessation
-
2009
- 2009-10-01 IL IL201315A patent/IL201315A/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
JP5368425B2 (ja) | 2013-12-18 |
EP2140034A1 (en) | 2010-01-06 |
AU2008237057A1 (en) | 2008-10-16 |
IL201315A0 (en) | 2010-05-31 |
JP2010523157A (ja) | 2010-07-15 |
IL201315A (en) | 2013-04-30 |
CN101680037B (zh) | 2012-12-05 |
ES2394384T3 (es) | 2013-01-31 |
US8298764B2 (en) | 2012-10-30 |
EP2140034B1 (en) | 2012-11-07 |
CN101680037A (zh) | 2010-03-24 |
WO2008124798A1 (en) | 2008-10-16 |
KR20100015558A (ko) | 2010-02-12 |
BRPI0810828A2 (pt) | 2014-10-29 |
CA2683602A1 (en) | 2008-10-16 |
EP2140034A4 (en) | 2011-04-06 |
US20090170711A1 (en) | 2009-07-02 |
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Legal Events
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FG | Grant or registration |