NL2030973B1 - Primer set for detection of expression of human eosinophil cationic protein (ecp) mrna and kit - Google Patents

Primer set for detection of expression of human eosinophil cationic protein (ecp) mrna and kit Download PDF

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NL2030973B1
NL2030973B1 NL2030973A NL2030973A NL2030973B1 NL 2030973 B1 NL2030973 B1 NL 2030973B1 NL 2030973 A NL2030973 A NL 2030973A NL 2030973 A NL2030973 A NL 2030973A NL 2030973 B1 NL2030973 B1 NL 2030973B1
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ecp
probe
mrna
kit
seq
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NL2030973A
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English (en)
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NL2030973A (en
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Wu Shandong
Jiang Xuehan
Wang Yifei
Liu Yi
Shen Huahao
Wang Jiaofeng
Cheng Lei
Wu Zhoujie
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Hangzhou Zheda Dixun Biological Gene Eng Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/106—Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Claims (8)

CONCLUSIES
1. Primerset voor detectie van een expressie van een humaan eo- sinofiel kationisch eiwit (ECP) mRNA, omvattende een ECP-F, een ECP-R, een GAPDH-F en een GAPDH-R; waarbij de ECP-F een nucleotideseduentie heeft die wordt getoond in SEQ ID
NO. 1, de ECP-R een nucleotidesequentie heeftdie wordt getoond in SEQ ID NO. 2, de GAPDH-F een nucleotidesequentie heeft die wordt getoond in SEQ ID NO. 3, en de GAPDH-R een nucleotidesequentie heeft die wordt getoond in SEQ ID NO. 4.
2. Primerset volgens conclusie 1, verder omvattende een probe E- Probe en een probe G-Probe; waarbij de E-Probe een nucleotideseguentie heeft die wordt getoond in SEQ ID NO. 5, en de G-Probe een nucleotidesequentie heeft die wordt getoond in SEQ ID NO. 6.
3. Primerset volgens conclusie 2, waarbij 5'-uiteinden van de pro- be E-Probe en de probe G-Probe beide zijn verbonden met een fluo- rescerende groep, en 3'-uiteinden van de probe E-Probe en de probe G-Probe beide zijn verbonden met een guenchgroep.
4. Primerset volgens conclusie 2 of 3, waarbij de 5'-uiteinden van de probes E-Probe en G-Probe zijn verbonden met verschillende flu- orescerende groepen, en de 3'-uiteinden van de probes E-Probe en G-Probe zijn verbonden met een zelfde quenchgroep.
5. Kit voor detectie in één stap van een expressie van een humaan ECP-mRNA, omvattende de primerset volgens een van de conclusies 1-
4.
6. Kit volgens conclusie 5, verder omvattende een PCR- reactieoplossing, een gemengde enzymoplossing, een carboxy-X- rhodamine (ROX) referentiekleurstof en nucleasevrij water; waarbij een enzym in de gemengde enzymoplossing een thermus aquaticus (Taq) enzym, een reverse transcriptase, een ribonuclease (RNase)-
remmer en een Tag-enzym antilichaam omvat met een massaverhouding van 13:5:3:1.
7. Kit volgens conclusie 6, waarbij de PCR-reactieoplossing een mengsel van deoxy-ribonucleosidetrifosfaat (dNTP) mengsel, MgCl. en een buffer omvat.
8. Kit volgens conclusie 5 of 6, verder omvattende een standaard van het humane ECP-mRNA.
NL2030973A 2021-08-04 2022-02-16 Primer set for detection of expression of human eosinophil cationic protein (ecp) mrna and kit NL2030973B1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202110891409.7A CN113373218A (zh) 2021-08-04 2021-08-04 一组检测人嗜酸性粒细胞阳离子蛋白mRNA表达的引物组和试剂盒

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NL2030973A NL2030973A (en) 2023-02-15
NL2030973B1 true NL2030973B1 (en) 2023-02-24

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CN (1) CN113373218A (nl)
AU (1) AU2021106592A4 (nl)
NL (1) NL2030973B1 (nl)
ZA (1) ZA202200493B (nl)

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WO2023010325A1 (zh) * 2021-08-04 2023-02-09 杭州浙大迪迅生物基因工程有限公司 一组检测人嗜酸性粒细胞阳离子蛋白mRNA表达的引物组、试剂盒和检测方法

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GB0111194D0 (en) * 2001-05-08 2001-06-27 Innoventus Uppsala Life Scienc Sequences
EP1549676A1 (en) * 2002-09-30 2005-07-06 Oncotherapy Science, Inc. Genes and polypeptides relating to human myeloid leukemia
US20160208246A1 (en) * 2013-06-10 2016-07-21 Yeda Research And Development Co. Ltd. Compositions and methods for treating a hematological malignancy associated with an altered runx1 activity or expression
GB201521357D0 (en) * 2015-12-03 2016-01-20 Univ Liverpool Methods for predicting response to anti-TNF therapy
WO2017125402A1 (en) * 2016-01-19 2017-07-27 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods of predicting drug responsiveness of patients suffering from autoimmune inflammatory diseases
WO2018178993A1 (en) * 2017-03-30 2018-10-04 Tel Hashomer Medical Research Infrastructure And Services Ltd. Diagnostic methods and kits for early detection of ovarian cancer
CN108220483A (zh) * 2018-02-12 2018-06-29 深圳出入境检验检疫局动植物检验检疫技术中心 一种用于罗非鱼湖病毒检测的试剂、检测方法及应用
WO2020190682A1 (en) * 2019-03-15 2020-09-24 Research Institute At Nationwide Children's Hospital Compositions and methods for diagnosing acute infectious peritonitis
CN110643740B (zh) * 2019-10-15 2023-08-04 云南省畜牧兽医科学院 帕利亚姆血清群病毒的实时荧光定量rt-pcr检测引物、探针及检测试剂盒
CN111304368A (zh) * 2020-03-19 2020-06-19 艾康生物技术(杭州)有限公司 用于检测新型冠状病毒的方法、寡核苷酸和试剂盒
CN111500773B (zh) * 2020-04-28 2023-09-29 云南省畜牧兽医科学院 一种流行性出血病病毒血清型鉴定荧光定量rt-pcr引物、探针和试剂盒

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CN113373218A (zh) 2021-09-10
AU2021106592A4 (en) 2021-11-11
NL2030973A (en) 2023-02-15
ZA202200493B (en) 2022-03-30

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