NL2024348B1 - Method for promoting breeding of larimichthys polyactis by artificial induction of labor - Google Patents
Method for promoting breeding of larimichthys polyactis by artificial induction of labor Download PDFInfo
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- NL2024348B1 NL2024348B1 NL2024348A NL2024348A NL2024348B1 NL 2024348 B1 NL2024348 B1 NL 2024348B1 NL 2024348 A NL2024348 A NL 2024348A NL 2024348 A NL2024348 A NL 2024348A NL 2024348 B1 NL2024348 B1 NL 2024348B1
- Authority
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- Netherlands
- Prior art keywords
- labor
- polyactis
- induction
- breeding
- labor induction
- Prior art date
Links
- 230000006698 induction Effects 0.000 title claims abstract description 41
- 241000356842 Larimichthys polyactis Species 0.000 title claims abstract description 34
- 238000009395 breeding Methods 0.000 title claims abstract description 20
- 230000001488 breeding effect Effects 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims abstract description 16
- 230000001737 promoting effect Effects 0.000 title claims abstract description 14
- 238000002347 injection Methods 0.000 claims abstract description 24
- 239000007924 injection Substances 0.000 claims abstract description 24
- 235000013601 eggs Nutrition 0.000 claims abstract description 11
- 239000004568 cement Substances 0.000 claims abstract description 8
- QLGKMLRGKPKGKI-QWQCQAQZSA-N (2s)-n-[(2s)-1-[[(2s)-1-[[(2s)-1-[[(2s)-1-[[(2r)-1-[[(2s)-1-[[(2s)-5-(diaminomethylideneamino)-1-[(2s)-2-(ethylcarbamoyl)pyrrolidin-1-yl]-1-oxopentan-2-yl]amino]-4-methyl-1-oxopentan-2-yl]amino]-1-oxopropan-2-yl]amino]-3-(4-hydroxyphenyl)-1-oxopropan-2-yl Chemical compound CCNC(=O)[C@@H]1CCCN1C(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](C)NC(=O)[C@@H](NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H]1NC(=O)CC1)CC1=CC=C(O)C=C1 QLGKMLRGKPKGKI-QWQCQAQZSA-N 0.000 claims abstract description 5
- 229940090044 injection Drugs 0.000 claims description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 241000251468 Actinopterygii Species 0.000 claims description 7
- 239000003814 drug Substances 0.000 claims description 7
- 210000002149 gonad Anatomy 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- 241000237502 Ostreidae Species 0.000 claims description 4
- 210000001015 abdomen Anatomy 0.000 claims description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 4
- 239000004744 fabric Substances 0.000 claims description 4
- 229940093181 glucose injection Drugs 0.000 claims description 4
- 238000005286 illumination Methods 0.000 claims description 4
- 235000013372 meat Nutrition 0.000 claims description 4
- 229910052760 oxygen Inorganic materials 0.000 claims description 4
- 239000001301 oxygen Substances 0.000 claims description 4
- 235000020636 oyster Nutrition 0.000 claims description 4
- 241000238371 Sepiidae Species 0.000 claims description 2
- 229940079593 drug Drugs 0.000 claims description 2
- 244000144987 brood Species 0.000 claims 3
- 230000004720 fertilization Effects 0.000 abstract description 3
- 230000012447 hatching Effects 0.000 abstract description 2
- 239000013535 sea water Substances 0.000 abstract description 2
- 238000005303 weighing Methods 0.000 abstract description 2
- 238000012258 culturing Methods 0.000 description 11
- 241000238366 Cephalopoda Species 0.000 description 3
- 241001596950 Larimichthys crocea Species 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 210000000582 semen Anatomy 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 241000276618 Perciformes Species 0.000 description 1
- 241001417494 Sciaenidae Species 0.000 description 1
- 241001663378 Sepiella maindroni Species 0.000 description 1
- 241000616933 Trichiurus haumela Species 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000009364 mariculture Methods 0.000 description 1
- 210000000006 pectoral fin Anatomy 0.000 description 1
- 235000014102 seafood Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
UITTREKSEL Disclosed is a method for promoting breeding of larimichthys polyactis by artificial induction of labor, which belongs to field of agricultural seawater seed breeding. In the disclosure, one— year—old larimichthys polyactis weighing 100—200 grams is used as 5 a broodstock for domestication and fortification. After fortification, diluted with LHRH—A2 and subjected, to one—time injection for induction of labor, the broodstock can lay eggs in a cement pit after 24—26 hours. With the technical steps in the disclosure, the larimichthys polyactis can successfully lay eggs 10 in an indoor cement pit, wherein the yield for induction of labor can reach 75%, and the hatching rate of fertilization rate reaches 80%, which can meet the needs of artificial breeding for the larimichthys polyactis.
Description
P569/NLpd
FIELD OF TECHNOLOGY The disclosure relates to a method for promoting breeding of larimichthys polyactis by artificial induction of labor, which be- longs to field of agricultural seawater seed breeding.
BACKGROUND Pseudosciaena polyactis Bleeker, also known as small fresh (Xiao Xian), yellow croaker, belongs to Perciformes, Sciaenidae. Pseudosciaena polyactis Bleeker is widely distributed in the East China Sea, the Yellow Sea and the Bohai Sea, and the offshore wa- ters on the west bank of the Korean Peninsula. It is a warm- temperature near-bottom fish. It has plays an important role in China's traditional marine fishery. Pseudosciaena polyactis Bleek- er, Larimichthys crocea, cuttlefish (Sepiella maindroni de Rochebrune), and Trichiurus haumela were once called “Four Sea- food“. However, since the 1970s, the production of Pseudosciaena polyactis Bleeker has been decreasing year by year due to factors such as overfishing and environmental degradation, forcing the price of adult Pseudosciaena polyactis Bleeker to soar. At pre- sent, the market price of chilled Pseudosciaena polyactis Bleeker is more than 80 yuan/kg, and it is in short supply. At the same time, Pseudosciaena polyactis Bleeker features growing fast, espe- cially suitable for artificial breeding. Therefore, artificial in- duction of labor for Pseudosciaena polyactis Bleeker is the prima- ry problem in the development of artificial breeding and cultiva- tion.
SUMMARY In order to solve above technical problems in the prior art, the disclosure provides a method for promoting breeding of larim- ichthys polyactis by artificial induction of labor includes forti- fied culturing of broodstock and injection of exogenous medicines for induction of labor, so as to promote the further development of the gonads of the broodstock, and cause larimichthys polyactis to naturally lay eggs in the indoor cement pit, thereby obtaining high-quality fertilized eggs. The disclosure provides technical support for the implementation of artificial breeding of larimich- thys polyactis.
In order to achieve above purpose, the present invention em- ploys the following technical solution: The method for promoting breeding of larimichthys polyactis by artificial induction of labor includes steps of: step one, indoor fortified culturing of broodstock: selecting one-year-old larimichthys polyactis as a broodstock, with a ratio of male to female of 2:1; step two: artificial induction of labor: a medicine for in- duction of labor is LHRH-A2, which is dissolved and diluted with a medical 6% glucose injection, with an injection dose of 1.25ug/kg for the females and half of the injection dose for the males; starting the injection at 5 to 6 in the evening by one-time injec- tion with an injection dose for induction of labor of 0.3-0.5mL per tail, then covering the entire pit with a black shade cloth to keep the surroundings quiet, so that the broodstock may lay eggs in the cement pit after 24 to 26 hours.
The method for promoting breeding of larimichthys polyactis by artificial induction of labor, wherein in the step one, a start time of the fortified culturing is February to March per year when an indoor water temperature reaches 10 °C or above.
The method for promoting breeding of larimichthys polyactis by artificial induction of labor, wherein conditions for the for- tified culturing are as follows: water temperature of 10-13 ° C, illumination of 1200LX, salinity of 30-35, dissolved oxygen of 5- 8mg/L, daily water exchange of 40%; a fodder made from oyster meat and squid pieces in a ratio of 1:1, which is fed twice a day with a feeding amount accounting for 3% of the weight of the brood- stock.
The method for promoting breeding of larimichthys polyactis by artificial induction of labor, wherein in the step two, a tim- ing for induction of labor is when the water temperature is 14 to
16 °C, the abdomen of the male may be squeezed to shed white se- men, and the female gonad has developed to stage IV as determined from anatomical observation; before induction of labor, the brood- stock is anesthetized with MS-222 of 15-20 ppm.
With above technical solution in the disclosure, the larim- ichthys polyactis can successfully lay eggs indoors, solving the problem of inability of laying eggs naturally during spawning pe- riod for one-year-old broodstock cultured indoors, wherein the yield for induction of labor can reach 75%, and the hatching rate of fertilization rate reaches 80%, which may meet the needs of ar- tificial breeding for the larimichthys polyactis.
DESCRIPTICN OF THE EMBODIMENTS The present invention will be further elaborated hereafter. In the disclosure, the method for promoting breeding of larimichthys polyactis by artificial induction of labor includes steps of:
1. Indoor fortified culturing of broodstock a start time of the fortified culturing is February to March per year when an indoor water temperature reaches 10 °C or above. One-year-old larimichthys polyactis is selected as a broodstock, with a ratio of male to female of 2:1. Method for managing the fortified culturing: water temperature of 10-13 ° C, illumination of 1200LX, salinity of 30-35, dissolved oxygen of 5-8mg/L, daily water exchange of 40%; a fodder made from oyster meat and squid pieces in a ratio of 1:1, which is fed twice a day with a feeding amount accounting for 3% of the weight of the broodstock.
2. Artificial induction of labor Timing for induction of labor: when the water temperature is 14 to 16 °C, the abdomen of the male may be squeezed to shed white semen, and the female gonad has developed to stage IV as deter- mined from anatomical observation; before induction of labor, the broodstock is anesthetized with MS-222 of 15-20 ppm.
Preparation and injection of medicine for induction of labor: a medicine for induction of labor is LHRH-A2, which is dissolved and diluted with a medical 6% glucose injection, with an injection dose of 1.25ug/kg for the females and half of the injection dose for the males; starting the injection at 5 to 6 in the evening by one-time injection with an injection dose for induction of labor of 0.3-0.5mL per tail and a ratio of male to female of 2:1, then covering the entire pit with a black shade cloth to keep the sur- roundings quiet, so that the broodstock may lay eggs in the cement pit after 24 to 26 hours. Embodiments From the beginning of March 2015 to the end of April 2015, a fortified culturing and artificial induction of labor for the broodstock were carried out in a 20 square indoor cement pit in a test site of Xishan Island of Zhejiang Mariculture Research Insti- tute, in which there were 200 broodstock each weighing 100-2009, and a total of 800 mL of high-quality fertilized eggs of larimich- thys polyactis were obtained. The test is specifically carried out as follows:
1. Indoor fortified culturing of broodstock Early in March, an indoor water temperature reaches 10 °C or above. One-year-old larimichthys polyactis is selected for forti- fied culturing, with a ratio of male to female of 2:1. Method for managing the fortified culturing: water temperature of 10-13 ° C, illumination of 1200LX, salinity of 30-35, dissolved oxygen of 5- 8mg/L, daily water exchange of 40%; a fodder made from oyster meat and squid pieces in a ratio of 1:1, which is fed twice a day with a feeding amount accounting for 33 of the weight of the brood- stock.
2. Artificial induction of labor In mid-April, the water temperature reaches 14 °C, the abdo- men of the male may be squeezed to shed white semen, and then the three females are dissected and observed to find that the gonads have developed to stage IV, so the induction of labor may be per-
formed.
Before induction of labor, the broodstock is anesthetized with MS-222 of 15-20 ppm.
Preparation and injection of medicine for induction of labor:
a medicine for induction of labor is LHRH-A2, which is dissolved
5 and diluted with a medical 6% glucose injection, with an injection dose of 1.25ug/kg for the females and half of the injection dose for the males; starting the injection at 5 to 6 in the evening at the origin of pectoral fin by one-time injection with an injection dose for induction of labor of 0.3-0.5mL per tail and a ratio of male to female of 2:1, then covering the entire pit with a black shade cloth to keep the surroundings quiet, so that the broodstock may lay eggs in the cement pit after 24 to 26 hours, finding that the yield for induction of labor is 75% and the fertilization rate is 80%.
Claims (4)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL2024348A NL2024348B1 (en) | 2019-11-29 | 2019-11-29 | Method for promoting breeding of larimichthys polyactis by artificial induction of labor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL2024348A NL2024348B1 (en) | 2019-11-29 | 2019-11-29 | Method for promoting breeding of larimichthys polyactis by artificial induction of labor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NL2024348B1 true NL2024348B1 (en) | 2021-08-31 |
Family
ID=77612488
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NL2024348A NL2024348B1 (en) | 2019-11-29 | 2019-11-29 | Method for promoting breeding of larimichthys polyactis by artificial induction of labor |
Country Status (1)
| Country | Link |
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| NL (1) | NL2024348B1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114568356A (en) * | 2022-03-17 | 2022-06-03 | 西藏自治区农牧科学院水产科学研究所 | Method for large-scale cultivation of salmon fries in eastern Asian province |
-
2019
- 2019-11-29 NL NL2024348A patent/NL2024348B1/en not_active IP Right Cessation
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114568356A (en) * | 2022-03-17 | 2022-06-03 | 西藏自治区农牧科学院水产科学研究所 | Method for large-scale cultivation of salmon fries in eastern Asian province |
| CN114568356B (en) * | 2022-03-17 | 2023-05-26 | 西藏自治区农牧科学院水产科学研究所 | Method for large-scale cultivation of salmon fries |
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| Date | Code | Title | Description |
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| MM | Lapsed because of non-payment of the annual fee |
Effective date: 20221201 |