CN114568356B - Method for large-scale cultivation of salmon fries - Google Patents

Method for large-scale cultivation of salmon fries Download PDF

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CN114568356B
CN114568356B CN202210264121.1A CN202210264121A CN114568356B CN 114568356 B CN114568356 B CN 114568356B CN 202210264121 A CN202210264121 A CN 202210264121A CN 114568356 B CN114568356 B CN 114568356B
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fish
fries
salmon
hatching
days
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CN114568356A (en
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王万良
陈美群
扎西拉姆
何文佳
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Institute of Animal Husbandry and Veterinary Medicine of Tibet Academy of Agriculture and Animal Husbandry Sciences
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Institute of Animal Husbandry and Veterinary Medicine of Tibet Academy of Agriculture and Animal Husbandry Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/10Culture of aquatic animals of fish
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/10Culture of aquatic animals of fish
    • A01K61/13Prevention or treatment of fish diseases
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/10Culture of aquatic animals of fish
    • A01K61/17Hatching, e.g. incubators
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

The invention provides a method for cultivating eastern salmon fries in large scale, which comprises the steps of screening female salmon and male salmon which are mature in development, carrying out artificial insemination by adopting an injection method 1 time after using MS-222 for anesthesia 2 days before egg extrusion, enabling an incubation water source to be groundwater, carrying out aeration oxygenation and ultraviolet sterilization, enabling each incubation tray to automatically flow to a vertical incubator from high to low, placing 9000-10000 fertilized eggs, incubating for 20-22 days, enabling fertilized eggs to fully appear eye spots, transferring the fertilized eggs into an incubation frame of a flat-row groove for incubation, and incubating for 41 days, and fully discharging membranes; when the yolk sac disappears, the body color of the fries becomes deep, the fries begin to feed with the micro-particle feed, after 15 days, the fries are transferred into the parallel grooves for cultivation, 1 ten thousand fries are placed in each parallel groove, and after 15 days of cultivation, the fries are transferred into a cement pond for cultivation. Realizing large-scale cultivation production from reproduction to fry cultivation of the salmon in the eastern region.

Description

Method for large-scale cultivation of salmon fries
Technical Field
The invention belongs to the technical field of fish culture, and particularly relates to a method for cultivating salmon fries in a large scale.
Background
Salmonids are one of the 3 large farmed fish in the world, with the farmed yield inferior to that after carp and tilapia. The culture areas of China are mainly distributed in places such as Heilongjiang, jilin, liaoning and Gansu, and the main varieties of culture include hucho salmon, rainbow trout, white salmon and red salmon. Eastern Salmon (Salmon trutta), also known as river Salmon, cat fish, belonging to the order Salmon, salmonidae, salmon genus, synonyms with the brown trout family, native to North Africa, europe and Western Asia, was introduced into eastern county by English people in the middle of the 19 th century, 150 years old from now. The eastern salmon establishes relatively stable herd in the Tibetan eastern river, can grow and breed naturally, becomes a local variety, belongs to cold water fish, is rich in nutrition, delicious in taste and deeply favored by consumers because the meat of the eastern salmon is tender and has no intramuscular thorns.
CN202110363281.7 discloses a method for large-scale propagation of eastern salmon in a pizza area, which comprises the steps of parent fish selection, artificial insemination, artificial hatching and fry opening, and the fertilization rate and the hatching rate are not high though the large-scale propagation of the eastern salmon is realized through strict control of artificial fertilization and hatching links.
Disclosure of Invention
In order to protect the resources of the Tibet plateau eastern salmon, the invention provides a method for cultivating the eastern salmon fries in a large scale, and realizes the large-scale cultivation production of the eastern salmon from propagation to fry cultivation.
The technical scheme adopted by the invention is as follows:
a method for cultivating salmon fries in large scale, comprising the following steps:
A. fertilized egg acquisition
Screening mature eastern salmon female fish and male fish, wherein the female fish is anesthetized by MS-222 2 days before egg extrusion and then injected for 1 time, the female fish uses an oxytocin LRH-A2 6 mug/kg+HCG 1000IU, the dosage of the male fish is one third of that of the female fish, the effect period of the female fish is 4-5 days, and the male fish is 2 days;
B. artificial insemination
Collecting semen 1 hr in advance, collecting semen at a dosage of 5 ml/tail, placing into a foam box containing ice cubes, keeping the temperature constant at 4deg.C, adopting semi-dry insemination mode, diluting with physiological saline, rapidly pouring onto eggs, clockwise stirring with feather for 15-20s, anticlockwise stirring for 15-20s, standing for 1min, and cleaning fertilized eggs with water until no mucus or foreign matter exists;
C. artificial hatching
The hatching water source is groundwater, after aeration oxygenation and ultraviolet sterilization, the hatching water source automatically flows to a vertical incubator from top to bottom, 9000-10000 fertilized eggs are placed on each hatching tray, and 100ppm formaldehyde is adopted for soaking 1 time every 2 days; after hatching for 20-22 days, the fertilized eggs are all at eye points, transferred into a flat-row groove hatching frame for hatching, and after 41 days of hatching, all the fertilized eggs are subjected to membrane removal;
D. opening for fish fry
When the yolk sac disappears, the body color of the fish fry is deepened, the feed of the micro-particles is started to be fed, and the fish fry is transferred into a flat-row groove for cultivation after 15 days; 1 ten thousand seedlings are placed in each parallel groove, and after 15 days of cultivation, the seedlings are transferred into a cement pond from the parallel grooves for cultivation.
Because the hatching frame is provided with the grooves, the residual bait and excrement are not easy to clean, and the residual bait and excrement must be moved into the flat-row groove after 15 days of cultivation, so that the residual bait and excrement are easy to manage and clean; and the fish is transferred into a cement pond from the parallel groove after 15 days, and the cement pond has large space, wide water body and sufficient exchange quantity, so that the environment is more suitable for the growth of the salmon fries in the east.
The step A of the invention is characterized in that the female fish of the eastern salmon is selected from 4 to 5 years old, and the method is characterized in that: the female fish belly is enlarged and soft, reproduction Kong Gongrun is prominent, when the caudal peduncles are lifted, the ovarian sagging profiles on the two sides are obvious, and eggs flow out of the body when individual individuals lightly press the belly; the male fish is 3-4 years old, and is characterized in that: mature Adonis salmon has bright body color and obvious red spots on the body surface, and the mouth and lower jaw are bent upwards to form barb projections, so that the abdomen of a male fish is gently extruded, and white semen flows out.
The vertical incubator has incubation water temperature of 9-10 ℃, dissolved oxygen of 6.5mg/L and water flow of 0.2-3L/s, and adopts micro-flow water for incubation.
In the step D, 1 ten thousand seedlings are placed in each parallel groove, the water flow is 0.5-0.6L/s, the dissolved oxygen is controlled to be more than 6.2mg/L, the cement pool is arranged in a room with a sunlight shed, and the cultivation density is 1000 tails/m 3 The water temperature is 11-13 ℃, the water flow is controlled at 4-5L/s, and the water exchange is ensured. If the water temperature increases, the water exchange amount decreases and the water quality deteriorates; an increase in the water exchange results in a relatively low water temperature, which is controlled to 11-13 c in combination.
The cement pond is cultivated, the self-made pellet feed is fed for 3 times per day, the dirt of residual bait at the bottom of the pond is cleaned for 1 time every 3 days, and the growth speed is high; the protein content of the self-made pellet feed is 44%, and the crude fat is 19%.
Preferably, the self-made pellet feed is a crushed material, and the mass percentage content of each component is as follows: 48% of fish meal, 4% of corn gluten meal, 3% of poultry byproduct meal, 3% of feather meal, 4% of bean pulp, 4% of blood meal, 18% of wheat flour, 4% of soybean oil, 10% of fish oil, 1.4% of compound vitamin and 0.6% of compound trace element. The feed can ensure the growth of fish fry.
The vertical incubator comprises a plurality of layers of incubation trays which are arranged up and down, incubation water sources are introduced into each layer of incubation tray through pipelines and discharged out, a movable incubation tray is arranged in each incubation tray, a plurality of incubation grooves are distributed on each movable incubation tray, a insemination egg can be just placed in each incubation groove, a water permeable hole is formed in the bottom of each incubation groove, and the water permeable holes are rectangular or elliptical.
Preferably, 9000-10000 hatching grooves are arranged on the movable hatching tray. The hatching tray plane is in a groove shape, and each groove just places one egg, so that certain water flow can be resisted. Eggs are not easy to be piled up by water flow, and the mutual pollution of water mould is not easy to be caused.
Preferably, the spawn collector is adopted to collect the inseminated spawn in the movable hatching tray, the spawn collector comprises an oviduct, an air supply pipe, a collecting bottle and a fan, the oviduct comprises a first oviduct and a second oviduct, the first oviduct is connected with the second oviduct and is at a certain angle, the end part of the second oviduct is connected with a three-way pipe, one end of the three-way pipe is connected with the air supply pipe, the other end of the three-way pipe is connected with the collecting bottle, the middle part of the three-way pipe is provided with a filter screen for preventing spawn from passing through by leaning against a passage opening of the air supply pipe, the end part of the air supply pipe is communicated with an air chamber, the top end of the air chamber is provided with a fan, the fan is forward and higher than the air supply pipe, the lower part of the air chamber is provided with a hollow handle, one side of the end part of the air supply pipe is obliquely downwards communicated with the handle, and the lower outlet of the handle is in threaded connection with a liquid collecting cylinder.
Further preferably, the end orifice of the first oviduct is provided with a soft sucker, and the inner diameter of the first oviduct increases from the end orifice to the other end.
The invention has the beneficial effects that:
1. according to the invention, through injection of the oxytocic, dosage is strictly controlled, the male fish collects semen 1h in advance, the temperature is kept at 4 ℃, and a semi-dry insemination mode is adopted, so that the fertilization rate can be ensured to be more than 96%; through the design of the feed for cultivating the fries and the control of protein, the survival rate of the fries reaches 91 percent, and the large-scale cultivation production from the reproduction of the salmon to the cultivation of the fries is realized.
2. According to the movable hatching tray, the plurality of hatching grooves are distributed on the movable hatching tray, one insemination egg can be just placed in each hatching groove, and the bottom of each hatching groove is provided with the water permeable hole, so that the insemination eggs can be well separated from each other, saprolegniasis can be effectively prevented, and the hatching rate is remarkably improved by more than 85%.
3. According to the invention, a requirement of placing one fertilized egg in each hatching groove is met, a fish egg collector is designed for overcoming the tedious workload, so that the fertilized eggs of dead eggs and developed eyespots can be conveniently picked out, the use is very convenient, the working efficiency is improved, and the large-scale cultivation and popularization of the eastern salmon are facilitated.
Drawings
Fig. 1 is a schematic view of the structure of a movable hatching tray according to the present invention.
Fig. 2 is a partially enlarged schematic view of a movable hatching tray according to the present invention.
FIG. 3 is a schematic diagram showing the structure of a fish egg collector of example 6.
FIG. 4 is a schematic diagram showing the structure of a fish egg collector according to example 7.
Reference numerals: 1. the movable hatching tray 2, the hatching groove 3, the permeable hole 4, the collecting bottle 5, the handle 6, the first oviduct 7, the second oviduct 8, the three-way pipe 9, the blast pipe 10, the air chamber 11, the liquid collecting cylinder 12, the fan 13, the filter screen 14, the fan switch 15, the sucking disc 16 and the holding slot.
Detailed Description
The invention will be further described by the following examples for the purpose of more clearly and specifically describing the object of the invention. The following examples are only for specific illustration of the implementation method of the present invention and do not limit the protection scope of the present invention.
Example 1
A method for cultivating salmon fries in large scale, comprising the following steps:
A. fertilized egg acquisition
Screening mature eastern salmon female fish and male fish, wherein the female fish is anesthetized by MS-222 2 days before egg extrusion and then injected for 1 time, the female fish uses an oxytocin LRH-A2 6 mug/kg+HCG 1000IU, the dosage of the male fish is one third of that of the female fish, the effect period of the female fish is 4-5 days, and the male fish is 2 days;
B. artificial insemination
Adopting a semidry insemination mode, diluting with normal saline, pouring onto eggs rapidly, stirring with feathers clockwise for 15-20s, stirring anticlockwise for 15-20s, standing for 1min, and cleaning fertilized eggs with water until no mucus or foreign matters exists, wherein the fertilization rate can be ensured to be more than 96% after 24 h.
According to 3 female and 3 male, semen is collected by male fish 1h in advance, the collection dosage is 5 ml/tail, the semen is placed into a foam box filled with ice cubes, the temperature is kept constant at 4 ℃, a semi-dry insemination mode is adopted, and 6.5% physiological saline is selected for dilution.
C. Artificial hatching
The hatching water source is groundwater, and after aeration oxygenation and ultraviolet sterilization, the hatching water flows from high to low to a vertical incubator, 9000-10000 fertilized eggs are placed on each hatching tray, and 100ppm formaldehyde is adopted for soaking 1 time every 2 days; after hatching for 20-22 days, the fertilized eggs are all at eye points, transferred into a flat-row groove for hatching, and after 41 days of hatching, all the fertilized eggs are subjected to membrane emergence;
the invention adopts a vertical incubator in CN202110363281.7.
D. Opening for fish fry
When the yolk sac disappears, the body color of the fish fry is deepened, the feed of the micro-particles is started to be fed, and the fish fry is transferred into a flat-row groove for cultivation after 15 days. 1 ten thousand seedlings are placed in each parallel groove, and after 15 days of cultivation, the seedlings are transferred into a cement pond from the parallel grooves for cultivation.
And (3) integrally transferring into a cement pond: and (3) quickly lifting the hatching frame, putting the hatching frame into a temporary water body, pulling the temporary water body to a flat-row groove by using a cart, and pouring the temporary water body. If a dip net is used, the fish is easily injured, and the skin of the small fish is fragile.
Example 2
This example is based on example 1:
the step A, the selection of the eastern salmon female fish is 4-5 years old, and is characterized in that: the female fish belly is enlarged and soft, reproduction Kong Gongrun is prominent, when the caudal peduncles are lifted, the ovarian sagging profiles on the two sides are obvious, and eggs flow out of the body when individual individuals lightly press the belly; the male fish is 3-4 years old, and is characterized in that: mature Adonis salmon has bright body color and obvious red spots on the body surface, and the mouth and lower jaw are bent upwards to form barb projections, so that the abdomen of a male fish is gently extruded, and white semen flows out.
Example 3
This example is based on example 1:
the hatching water temperature of the vertical incubator is 9-10 ℃, the dissolved oxygen is 6.5mg/L, and the water flow is 0.2-3L/s.
In the step D, 1 ten thousand seedlings are placed in each parallel groove, the water flow is 0.5-0.6L/s, the dissolved oxygen is controlled to be more than 6.2mg/L, the cement pond is arranged in a room with a sunlight shed, and the cultivation density is 1000 tails/m 3 The water temperature is 11-13 ℃, and the water flow is controlled to be 4-5L/s.
The cement pond is cultivated, the feeding feed is self-made pellet feed, the feeding is carried out 3 times a day, the dirt on the residual bait at the bottom of the pond is cleaned 1 time every 3 days, and the growth speed is high.
The protein content of the self-made pellet feed is 44%, and the crude fat is 19%. The self-made pellet feed is a crushed material, and comprises the following components in percentage by mass: 48% of fish meal, 4% of corn gluten meal, 3% of poultry byproduct meal, 3% of feather meal, 4% of bean pulp, 4% of blood meal, 18% of wheat flour, 4% of soybean oil, 10% of fish oil, 1.4% of compound vitamin and 0.6% of compound trace element.
Example 4
This example is based on example 1:
the vertical incubator comprises a plurality of layers of incubation trays which are arranged up and down, incubation water sources are introduced into each layer of incubation tray through pipelines and discharged out, a movable incubation tray 1 is arranged in each incubation tray, a plurality of incubation grooves 2 are distributed on each movable incubation tray 1, an insemination egg can be just placed in each incubation groove 2, and a water permeable hole 3 is formed in the bottom of each incubation groove.
The hatching tray is 60cm multiplied by 50cm, fertilized eggs are placed for 9000-10000 grains, and 100ppm formaldehyde is adopted for soaking 1 time every 2 days (due to long hatching period, no water mould can be ensured to occur); after hatching for 20-22 days, fertilized eggs are all at eye points, and then transferred into a flat-row groove for hatching by a vertical incubator, wherein the flat-row groove is 2m multiplied by 0.5m multiplied by 0.4m. Fertilized eggs were hatched for 41 days and all the fertilized eggs were removed from the membranes. After the eyes develop, the dead eggs and other dirt are cleaned off in time every day, so that the water quality is ensured to be fresh. After being put in, the utility model can be automatically arranged, and then the utility model can be put in order and standard by lightly pushing the utility model with feathers.
The structure of the vertical incubator is shown in CN202110363281.7.
Example 5
This example is based on example 4:
the water permeable holes 3 are rectangular or oval, so that the blocking of round fish eggs is avoided.
9000-10000 hatching grooves 2 are arranged on the movable hatching tray 1.
Example 6
This example is based on example 4:
as shown in fig. 3, a fish egg collector is used for collecting fertilized eggs in a movable hatching tray 1, the fish egg collector comprises an oviduct, an air supply pipe 9, a collecting bottle 4 and a fan 12, the oviduct comprises a first oviduct 6 and a second oviduct 7, the first oviduct 6 is connected with the second oviduct 7 and is at a certain angle, the end part of the second oviduct 6 is connected with a three-way pipe 8, one end of the three-way pipe 8 is connected with the air supply pipe 9, the other end of the three-way pipe 8 is connected with the collecting bottle 4, a filter screen 13 for preventing the passage of fish eggs is arranged in the middle of the three-way pipe 8 and is close to a passage opening of the air supply pipe 9, the end part of the air supply pipe 9 is communicated with an air chamber 10, the fan 12 is arranged at the top end of the air chamber 10, the fan 10 is forward and higher than the air supply pipe 9, a hollow handle 5 is arranged below the air chamber 10, one side of the end part of the air supply pipe 9 is obliquely communicated with the handle 5 downwards, and a lower outlet of the handle 5 is in threaded connection with a liquid collecting cylinder 11.
After 20-22 days of incubation, fertilized eggs are all at eye points, and picked out by a fish egg collector and transferred into a flat-row groove incubation frame for incubation.
When the fish egg collector is used, the first oviduct 6 is aligned with one hatching groove 2, the fan 12 is turned on to generate negative pressure, fish eggs and water are sucked into the first oviduct 6 together, the fish eggs fall into the collecting bottle 4 below from the three-way pipe 8 under the action of gravity through the second oviduct 7, the middle part of the three-way pipe 8 is provided with the filter screen 13 towards the passage opening of the air supply pipe 9, the fish eggs are prevented from being sucked by mistake, a small amount of water enters the air supply pipe 9, flows downwards into the handle 5 from the inclined surface of the air supply pipe 9, and is prevented from being sucked into the fan 12 to influence the operation of the fan 12.
The position of the fan 12 is higher than the blast pipe 9 to further prevent water from being sucked, and a small amount of residual liquid enters the liquid collecting cylinder 11 below from the handle 5, so that the residual liquid can be cleaned by detaching the liquid collecting cylinder 11.
Example 7
This example is based on example 6:
as shown in fig. 4, the end orifice of the first oviduct 6 is provided with a soft sucker 15, and the inner diameter of the first oviduct 6 increases from the end orifice to the other end.
The soft sucker 15 prevents the fish eggs from being damaged, and simultaneously, the orifice diameter of the first oviduct 6 is enlarged to be more beneficial to inhalation. The inner diameter of the first oviduct 6 from the end pipe orifice is smaller than that of the other end, so that the negative pressure of the suction inlet is increased, the negative pressure close to the fan end is reduced on the premise that the roe can be sucked rapidly, and the water is prevented from being sucked into the fan.
Three batches of parent fish are collected in the Lassa area 2021 in 1-2 months, the method and the movable hatching tray are used for artificial hatching according to the invention, and a fish egg collector is used for collecting picked eggs, and specific propagation conditions are shown in tables 1 and 2:
TABLE 1 Artificial fertilization and hatching conditions for Adonis salmon in the Lassa area
Figure 337150DEST_PATH_IMAGE001
The breeding experiment is carried out 3 months in 2021, 0.4g of salmon fries are selected, the salmon fries are bred for 30 days, self-made pellet feed is adopted, the salmon fries are fed for 3 times a day, the standard (the feeding amount of the salmon fries is 7-8% under the weight of 0.4g, the feeding amount of the salmon fries is 5-6% under the weight of 0.4g, and the feeding amount of the salmon fries is 3-4% over 0.7) is fed for 0.4-0.6g, and the dirt of residual bait at the bottom of a pond is cleaned for 1 time every 3 days. The results show that the protein has the highest weight gain rate at 44%, the highest growth rate and the lowest death rate.
TABLE 1 growth of Adonis salmon fries in the Lassa area
Figure 628454DEST_PATH_IMAGE002
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The foregoing examples merely illustrate specific embodiments of the invention, which are described in greater detail and are not to be construed as limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention.

Claims (5)

1. A method for large-scale cultivation of salmon fries, which is characterized by comprising the following steps:
A. fertilized egg acquisition
Screening mature eastern salmon female fish and male fish, wherein the female fish is anesthetized by MS-222 2 days before egg extrusion and then injected for 1 time, the female fish uses an oxytocin LRH-A2 6 mug/kg+HCG 1000IU, the dosage of the male fish is one third of that of the female fish, the effect period of the female fish is 4-5 days, and the male fish is 2 days;
B. artificial insemination
Collecting semen 1 hr in advance, collecting semen at a dosage of 5 ml/tail, preserving at 4deg.C, adopting semi-dry insemination mode, diluting with normal saline, rapidly pouring onto ovum, clockwise stirring with feather for 15-20s, anticlockwise stirring for 15-20s, standing for 1min, and cleaning fertilized ovum with water until there is no mucus and foreign body;
C. artificial hatching
The hatching water source is groundwater, after aeration oxygenation and ultraviolet sterilization, the hatching water flows from high to low to a vertical incubator, 9000-10000 fertilized eggs are placed on each hatching tray, and 100ppm formaldehyde is adopted for soaking 1 time every 2 days; after hatching for 20-22 days, the fertilized eggs are all at eye points, transferred into a flat-row groove hatching frame for hatching, and after 41 days of hatching, all the fertilized eggs are subjected to membrane removal;
D. opening for fish fry
When the yolk sac disappears, the body color of the fries becomes deep, the fries begin to feed with microparticle feed, after 15 days, the fries are transferred into parallel grooves for cultivation, 1 ten thousand fries are placed in each parallel groove, and after 15 days of cultivation, the fries are transferred into a cement pond for cultivation;
the hatching water temperature of the vertical incubator is 9-10 ℃, the dissolved oxygen is 6.5mg/L, and the water flow is 0.2-3L/s;
the vertical incubator comprises a plurality of layers of incubation trays which are arranged up and down, incubation water sources are introduced into each layer of incubation tray through pipelines and discharged out, a movable incubation tray is arranged in each incubation tray, a plurality of incubation grooves are distributed on each movable incubation tray, a insemination egg can be just placed in each incubation groove, a water permeable hole is formed in the bottom of each incubation groove, and the water permeable holes are rectangular or elliptical;
9000-10000 hatching grooves are formed in the movable hatching tray;
the method comprises the steps that a fish egg collector is adopted to collect fertilized eggs in a movable hatching disc, the fish egg collector comprises an oviduct, an air supply pipe, a collecting bottle and a fan, the oviduct comprises a first oviduct and a second oviduct, the first oviduct is connected with the second oviduct and is at a certain angle, the end part of the second oviduct is connected with a three-way pipe, one end of the three-way pipe is connected with the air supply pipe, the other end of the three-way pipe is connected with the collecting bottle, a filter screen for preventing the fish eggs from passing through is arranged in the middle of the three-way pipe, the end part of the air supply pipe is communicated with an air chamber, a fan is arranged at the top end of the air chamber, the fan is forward and is higher than the air supply pipe, a hollow handle is arranged below the air chamber, one side of the end part of the air supply pipe is obliquely downwards communicated with the handle, and a liquid collecting cylinder is connected with an outlet thread below the handle; the end orifice of the first oviduct is provided with a soft sucker, and the inner diameter of the first oviduct from the end orifice to the other end increases gradually.
2. The method for large-scale cultivation of the seedlings of the eastern salmon according to claim 1, wherein in the step a, the female fish of the eastern salmon is selected from 4 to 5 years old, and is characterized in that: the female fish belly is enlarged and soft, reproduction Kong Gongrun is prominent, when the caudal peduncles are lifted, the ovarian sagging profiles on the two sides are obvious, and eggs flow out of the body when individual individuals lightly press the belly; the male fish is 3-4 years old, and is characterized in that: mature Adonis salmon has bright body color and obvious red spots on the body surface, and the mouth and lower jaw are bent upwards to form barb projections, so that the abdomen of a male fish is gently extruded, and white semen flows out.
3. The method for cultivating the salmon fries on a large scale according to claim 1, wherein in the step D, 1 ten thousand fries are placed in each flat-row groove, the water flow is 0.5-0.6L/s, and the dissolved oxygen is controlled to be more than 6.2 mg/L; the cement pond is arranged in a room with a sunlight shed, and the cultivation density is 1000 tails/m 3 The water temperature is 11-13 ℃, and the water flow is controlled to be 4-5L/s.
4. The method for cultivating the salmon fries on a large scale according to claim 1, wherein the cement pond is cultivated, the feed is self-made granular feed, the feed is fed for 3 times a day, and residual bait dirt at the bottom of the pond is cleaned for 1 time every 3 days; the protein content of the self-made pellet feed is 44%, and the crude fat is 19%.
5. The method for cultivating the salmon fries on a large scale according to claim 4, wherein the self-made pellet feed is a crushed material, and the mass percentage of each component is as follows: 48% of fish meal, 4% of corn gluten meal, 3% of poultry byproduct meal, 3% of feather meal, 4% of bean pulp, 4% of blood meal, 18% of wheat flour, 4% of soybean oil, 10% of fish oil, 1.4% of compound vitamin and 0.6% of compound trace element.
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