CN107135989A - A kind of artificial breeding method of neat mouth schizothoracin fry - Google Patents
A kind of artificial breeding method of neat mouth schizothoracin fry Download PDFInfo
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 121
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- OSVXSBDYLRYLIG-UHFFFAOYSA-N dioxidochlorine(.) Chemical compound O=Cl=O OSVXSBDYLRYLIG-UHFFFAOYSA-N 0.000 claims description 6
- 230000009027 insemination Effects 0.000 claims description 6
- 230000013011 mating Effects 0.000 claims description 5
- 235000019750 Crude protein Nutrition 0.000 claims description 4
- 241001439627 Schistostoma Species 0.000 claims description 4
- 230000037396 body weight Effects 0.000 claims description 4
- 201000010099 disease Diseases 0.000 claims description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 4
- 239000004744 fabric Substances 0.000 claims description 4
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- 230000002265 prevention Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- NHYCGSASNAIGLD-UHFFFAOYSA-N Chlorine monoxide Chemical compound Cl[O] NHYCGSASNAIGLD-UHFFFAOYSA-N 0.000 description 1
- 241000252210 Cyprinidae Species 0.000 description 1
- 241001275867 Schizothorax Species 0.000 description 1
- 241001275865 Schizothorax prenanti Species 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a kind of artificial breeding method of neat mouth schizothoracin fry, by parent fish rearing, parent population selection with combo, artificial induced spawning, fertilization, incubating oosperm, prelarva temporarily support, fry rearing the step of, neat mouth schizothoracin has successfully been bred, the technological gap is filled up.The present invention can be concentrated, the neat mouth schizothoracin fry of the acquisition of batch, and spawning rate, rate of fertilization, incubation rate are high, be advantageously implemented the large-scale production of neat mouth schizothoracin, and the protection for the mouth schizothoracin wild resource that aligns and large-scale cultivation are significant.
Description
Technical field
The invention belongs to technical field of aquaculture, a kind of artificial breeding side of neat mouth schizothoracin fry is specifically related to
Method.
Background technology
It is sub- that neat mouth schizothoracin (Schizothoraxprenanti) is subordinate to Cyprinidae, Schizothoracinae, Schizothorax, schizothoracin
Category, is distributed mainly on the waters such as Jinsha jiang River, Ming River, Dadu River, Qingyi River and the Lower Reaches of Wujiang River of Upper Yangtze River, is the rare of producing region
Fish and high-quality fingerling.Neat mouth schizothoracin individual is larger, can typically grow to 1~3kg, maximum individual up to 8kg, due to it is a variety of because
Element, neat mouth schizothoracin natural resources amount significantly exhaustion.
Neat mouth schizothoracin is rare fish and the high-quality fingerling in producing region, and its fine and tender taste, delicious flavour is nutritious, deep
Liked by consumer, with larger economic value.Occur serious fragmentation recently as original producton location aquatic ecosystem,
In addition the factor such as hydraulic engineering construction, water body environment pollution and overfishing, wild resource drastically declines, and this precious fingerling is on the point of
Face extinction.For this Species of Rare Fish from Qingdao resource of protection and developmental utilization, many scholars have carried out in terms of biology, artificial domestication
Research.Inventor achieves the success of neat mouth schizothoracin artificial propagation test by studying intensively for many years.
The content of the invention
It is an object of the invention to provide a kind of artificial breeding method of neat mouth schizothoracin fry, can concentrate, the acquisition of batch it is neat
Mouth schizothoracin fry.
In order to solve the above technical problems, the technical solution adopted in the present invention is：A kind of neat mouth schizothoracin fry it is artificial
Mating system, is concretely comprised the following steps：
1) parent fish rearing：Parent fish rearing pond is flowing water pond, and breeding density is 1-3 tails/m2, the nurturing period, determine matter, fixed point,
Regularly, quantitatively fed；Determine matter：Feed crude protein content 32%~35%, calorie-protein ratio (E/P) 36kJ/g~40kJ/g
Expanded pellet feed and/or water earthworm living bait；Fixed point：Feedstuff feeding, which should uniformly be thrown, is spread on the FX of fishpond one, every time
Feeding time 25-35min；Regularly：Day feeds number of times and determined according to water temperature situation of change, and is relatively fixed the time；It is quantitative：Day throws
Bait rate is the 2~3% of fish body weight；Day feeding rate should rationally determine according to water temperature, body size, and according to water quality, water temperature, day
The biomass of fish and situation of ingesting are adjusted in right amount in gas, culturing pool；
2) parent population selection and combo：4 more than age sexually matured neat schistostoma abdomen fish parent fish are selected, physique healthy and strong, not damaged are pressed
The combo of female-male proportion 1.5: 1；
3) artificial induced spawning：In neat mouth schizothoracin mating period, sexually matured parent population, injection interstitialcellstimulating hormone (ICSH) release are selected
Hormone analogs, are then put into spawning inducing pool by parent population, and ripe fish density is 1 group/m2, stimulation by running water is kept in pond；
4) inseminate：After hastening parturition, parent population active situation is observed, it is found that parent population has obvious estrus behavior, draws in the net to check parent population, gently
Pressure raun belly has ovum grain outflow, using dry method of artificial fertilization；
5) incubating oosperm：Incubator is positioned in hatching tank, and bottom is away from water surface 10-15cm, after artificial insemination
Embryonated egg, which is laid in incubator, hatches, and Spawning water is through filtration treatment, dissolved oxygen >=6.0mg/L, pH neutrality or alkalescence, water temperature
15-18℃；
6) prelarva is temporarily supported：After incubating oosperm membrane, prelarva is put into hatching tank and temporarily supported, fry floats flat after swimming turns
Enter fry rearing pond；
7) fry rearing：Micro- running water technology, fry rearing pond water velocity 1-2cm/s are taken, fry breeding density is
1500-2000 tails/m2, open-mouthed bait is water earthworm, when fry body length reaches 2cm, progressively tames and dociles food with mixed feed；Remove in time
Residual bait and dirt, are inspected periodically, it is found that disease is treated in time；
By above-mentioned steps, the artificial breeding of neat mouth schizothoracin fry is completed.
The parent fish rearing pond and fry rearing pond are rectangle, and one end sets water inlet, and the other end sets discharge outlet,
Discharge outlet sets spilling water station pipe, and drainage mouthful is respectively provided with escaping-preventing facilities；Surface water inlet, the mode of bottom discharge is taken to enter water-filling
Body is exchanged.
The water control in parent fish rearing pond is exchanged 5-8 times daily, dissolved oxygen >=6.0mg/L, pH value neutrality or meta-alkalescence, thoroughly
Lightness >=50cm；And bred first 2 months in parent population, timing increases bath and stimulated, and flow velocity is 3-5cm/s.
Step 4) described in obvious estrus behavior be heat climax；Mutually compel between proestrus group, female milter by milter
Between vie each other；Middle and later periods, female milter intermittently mutually answers back to other side's belly with rhynchodaenm；Later stage, female fish swimming is slow, etc.
Milter is treated, when female milter abuts side by side, raun body drastically trembles, this is spawning sign, i.e. heat climax.
Hatching tank is rectangular-shape, and one end sets water inlet, and other end bottom sets osculum and drainage pipeline；The depth of water is protected
Hold in 30-35cm, incubator is wood frame, bottom and surrounding are tulle cloth.
Step 5) in hatching process, miniflow water is kept, incubator is shaken in water flow velocity 1-2cm/s, timing, and rejects water mold
Ovum.
The fry rearing pond locally sets shadow shield, prevents strong light direct beam, takes micro- running water technology, have abundant water resources, water
Waterflow stabilization is measured, water quality is pure and fresh；Results of regular determination physicochemical factors；Fry rearing early stage 1-2d suctions are dirty 1 time, middle and later periods, 3-
5d suctions are dirty 1 time.
Parent fish rearing pond, spawning inducing pool, hatching tank, incubator and fry rearing pond, the 2-4d 5ppm bis- before use
Chlorine monoxid or 20ppm Betagen Solutions immersion 24h, come into operation after cleaning.
The depth of water in parent fish rearing pond is 120-180cm, and the depth of water of spawning inducing pool is maintained at the depth of water in 60-70cm, hatching tank
30-35cm is maintained at, the depth of water in fry rearing pond is in 50-100cm.
The size of the hatching tank length, width and height is 3.0m × 0.8m × 0.4m, and the size of the length, width and height of incubator is 0.6m
3-5 incubator is placed in × 0.4m × 0.2m, hatching tank.
The artificial breeding method for the neat mouth schizothoracin fry that the present invention is provided, spawning rate, rate of fertilization, incubation rate, fry into
Motility rate is high, can concentrate, the neat mouth schizothoracin fry of acquisition of batch, is advantageously implemented the large-scale production of neat mouth schizothoracin, aligns
The protection of mouth schizothoracin wild resource and large-scale cultivation are significant.
The present invention's focuses on neat mouth schizothoracin artificial breeding method, and in detail, implementation result is good for technical scheme science：
Parent fish rearing, summarizes in terms of culture pond condition, cultivation density, feedstuff feeding technology, parent population selection and proposes system
Mature technology, it is possible to increase the maturity and fish brood amount of parent population, cultivates high-quality parent population.Artificial induced spawning, optimizes oxytocic drug, favorably
In protection parent population, science is feasible, and cost-effective, spawning effect is good.Incubating oosperm, hatchery facility is reasonable in design, easy to operate, incubates
Rate is high.Fry rearing, takes micro- running water technology, and fry rearing pond locally sets shadow shield, prevents strong light direct beam, is nursery training
The key measure educated.
Embodiment
The present invention is further illustrated with reference to embodiment, but the scope of protection of present invention is not limited to implement
The scope of example statement.
A kind of artificial breeding method of neat mouth schizothoracin fry, specifically includes following steps：
1) parent fish rearing：Parent fish rearing pond is flowing water pond, and breeding density is 1-3 tails/m2, the nurturing period, determine matter, fixed point,
Regularly, quantitatively fed；Determine matter：Feed crude protein content 32%~35%, calorie-protein ratio (E/P) 36kJ/g~40kJ/g
Expanded pellet feed and/or water earthworm living bait；Fixed point：Feedstuff feeding, which should uniformly be thrown, is spread on the FX of fishpond one, every time
Feeding time 25-35min；Regularly：Day feeds number of times and determined according to water temperature situation of change, and is relatively fixed the time；It is quantitative：Day throws
Bait rate is the 2~3% of fish body weight；Day feeding rate should rationally determine according to water temperature, body size, and according to water quality, water temperature, day
The biomass of fish and situation of ingesting are adjusted in right amount in gas, culturing pool；
2) parent population selection and combo：4 more than age sexually matured neat schistostoma abdomen fish parent fish are selected, physique healthy and strong, not damaged are pressed
The combo of female-male proportion 1.5: 1；
3) artificial induced spawning：In neat mouth schizothoracin mating period, sexually matured parent population, injection interstitialcellstimulating hormone (ICSH) release are selected
Hormone analogs, are then put into spawning inducing pool by parent population, and ripe fish density is 1 group/m2, stimulation by running water is kept in pond；
4) inseminate：After hastening parturition, parent population active situation is observed, it is found that parent population has obvious estrus behavior, light pressure raun belly has
Ovum grain outflow, using dry method of artificial fertilization；
5) incubating oosperm：Incubator is positioned in hatching tank, and bottom is away from water surface 10-15cm, after artificial insemination
Embryonated egg, which is laid in incubator, hatches, and Spawning water is through filtration treatment, dissolved oxygen >=6.0mg/L, pH neutrality or alkalescence, water temperature
15-18℃；
6) prelarva is temporarily supported：After incubating oosperm membrane, prelarva is put into hatching tank and temporarily supported, fry floats flat after swimming turns
Enter fry rearing pond；
7) fry rearing：Fry rearing pond water velocity 1-2cm/s, fry breeding density is 1500-2000 tails/m2, open
Mouth bait is water earthworm, when fry body length reaches 2cm, progressively tames and dociles food with mixed feed；Residual bait and dirt are removed in time, periodically inspection
Look into, it is found that disease is treated in time；
By above-mentioned steps, the artificial breeding of neat mouth schizothoracin fry is completed.
Further, the parent fish rearing pond and fry rearing pond are rectangle, and one end sets water inlet, and the other end is set
Discharge outlet is put, discharge outlet sets spilling water station pipe, and drainage mouthful is respectively provided with escaping-preventing facilities；Take surface water inlet, the side of bottom discharge
Formula carries out water body exchange.
Further, the water control in parent fish rearing pond is exchanged 5-8 time daily, dissolved oxygen >=6.0mg/L, pH value neutrality or
Meta-alkalescence, transparency >=50cm；And bred first 2 months in parent population, timing increases bath and stimulated.
Further, step 4) described in obvious estrus behavior be heat climax；Between proestrus group, female milter mutually
Compel by being vied each other between milter；Middle and later periods, female milter intermittently mutually answers back to other side's belly with rhynchodaenm；Later stage, raun trip
It is dynamic slow, milter is waited, when female milter abuts side by side, raun body drastically trembles, this is spawning sign, i.e. heat climax.
Further, hatching tank is rectangular-shape, and one end sets water inlet, and other end bottom sets osculum and drainpipe
Road；The depth of water is maintained at 30-35cm, and incubator is wood frame, and bottom and surrounding are tulle cloth.
Further, step 5) in hatching process, miniflow water is kept, incubator is shaken in timing, and rejects water mold ovum.
Further, the fry rearing pond locally sets shadow shield, prevents strong light direct beam, has abundant water resources, water current
Stable, water quality is pure and fresh；Results of regular determination physicochemical factors；Fry rearing early stage 1-2d suctions are dirty 1 time, middle and later periods, 3-5d suctions
It is dirty 1 time.
Further, parent fish rearing pond, spawning inducing pool, hatching tank, incubator and fry rearing pond, the 2-4d before use
With 5ppm chlorine dioxide or 20ppm Betagen Solutions immersion 24h, come into operation after cleaning.
Further, the depth of water in parent fish rearing pond is 120-180cm, and the depth of water of spawning inducing pool is maintained at 60-70cm, hatches water
Trough inner water is maintained at 30-35cm deeply, and the depth of water in fry rearing pond is in 50-100cm.
Further, the size of the hatching tank length, width and height is 3.0m × 0.8m × 0.4m, the length, width and height of incubator
Size is to place 3-5 incubator in 0.6m × 0.4m × 0.2m, hatching tank.
Embodiment 1：
(1) parent fish rearing：The special pond in flowing water pond is taken to cultivate, area 50-200m2, well, water quality is pure and fresh without dirt for water condition
Dye, water is sufficient.Artificial cave is set, perched beneficial to parent population when sunlight is stronger.The preceding 3-4d that parent population is put in a suitable place to breed, uses 5ppm dioxies
Change chlorine or 20ppm Betagen Solutions immersion 24h, then clean up, be then placed in natural river water, water level is 100-180cm.Parent
Fish breeding density is 1-3 tails/m2.Micro- flowing water culture, water control is exchanged 5-8 times daily, dissolved oxygen >=6.0mg/L, in pH value
Property or meta-alkalescence, transparency >=50cm.Nurturing period, adhere to that " determine matter, fixed point, timing, quantitative " feeds principle.Determine matter：Feed
Crude protein content 32%~35%, calorie-protein ratio (E/P) 36kJ/g~40kJ/g expanded pellet feed and/or water earthworm are lived
Bait；Fixed point：Feedstuff feeding, which should uniformly be thrown, is spread on the FX of fishpond one, each Feeding time 30min or so.Regularly：Day throws
Feed number of times to be determined according to water temperature situation of change, and be relatively fixed the time.It is quantitative：Day feeding rate is the 2~3% of fish body weight；Day throws
Bait rate should rationally determine according to water temperature, body size, and biomass according to fish in water quality, water temperature, weather, culturing pool and ingest
Situation is adjusted in right amount.First 2 months of breeding, the appropriate increased water flow of timing is stimulated, and promotes gonad development.
(2) parent population selection and combo：Select 4 more than age sexually matured neat schistostoma abdomen fish parent fish, physique healthy and strong, not damaged.
By the combo of female-male proportion 1.5: 1.
(3) artificial induced spawning：In neat mouth schizothoracin mating period, sexually matured parent population is selected, injection interstitialcellstimulating hormone (ICSH) is released
Hormone analogs are put, induce it to lay eggs and row's essence.Inject after oxytocic drug, parent population is put into spawning inducing pool, and it is 1 that parent population, which delivers density,
Group/m2.The spawning inducing pool depth of water is maintained at 60-70cm, keeps certain stimulation by running water.
(4) inseminate：Fertilization method takes artificial insemination.After hastening parturition, when being expected to reach Effect time, observation parent population activity
Situation, it is found that parent population has obvious estrus behavior, draws in the net to check parent population, light pressure raun belly has ovum grain outflow, you can use dry method
Carry out artificial insemination.
(5) incubating oosperm：Incubating oosperm includes hatchery facility and hatching management.Hatchery facility：Hatch tank, hatching
Case.Hatch tank (1.5m × 0.7m × 0.4m), tank front end sets spray type to intake, and rear end bottom sets osculum and draining
Pipeline.The depth of water is maintained at 35cm or so in hatching tank.Incubator (0.6m × 0.4m × 0.2m), incubator is wood frame,
Bottom and the tulle cloth that surrounding is 40 mesh.3 incubators of arrangement in each hatching tank.Hatching management：Incubator, which is positioned over, incubates
Change in tank, embryonated egg is put into incubator and hatched away from water surface 15cm or so by incubator bottom, and ovum is put in each incubator
4000~5000, embryonated egg is evenly laid out in incubator.Spawning water passes through in filtration treatment, dissolved oxygen >=6.0mg/L, pH
Property or alkalescence, 13-18 DEG C of water temperature, avoid direct sunlight at shading.Holding miniflow water in hatching process, timing jog incubator,
Prevent embryonated egg from accumulating.Water mold ovum is rejected, saprolegniasis is controlled.
(6) prelarva is temporarily supported：After the whole incubation of membrane of embryonated egg in incubator, prelarva is put into hatching tank and temporarily supported,
Fry rearing pond is transferred to after the flat trip of fry floating.
(7) fry rearing：Select cement culture pond, area 20-80m2, depth of water 50-100cm, the one of rearing pond
End sets water inlet, and the other end sets discharge outlet.Discharge outlet sets spilling water station pipe, controls culturing pool water level.Enter discharge outlet setting
Escaping-preventing facilities.Culturing pool enters draining and takes surface water inlet, the mode of bottom discharge, is exchanged for water body.Culture pond is locally set
Shadow shield, prevents strong light direct beam.Have abundant water resources, water waterflow stabilization, water quality is pure and fresh.Fry breeding density be 1500-2000 tails/
m2, open-mouthed bait is water earthworm, when fry body length reaches 2cm, progressively tames and dociles food with mixed feed.Keep miniflow water, water velocity
1-2cm/s.Keep suitable water level, results of regular determination physicochemical factors.Residual bait and dirt, fry rearing early stage 1- are removed in time
2d suctions are dirty 1 time.Middle and later periods, 3-5d suctions are dirty 1 time.Fish disease is prevented：Adhere to the principle of " putting prevention first and combining prevention with control ", instrument
Strict sterilization is answered, culture pond periodically uses disinfection by chlorine dioxide.Careful operation, it is to avoid fish body is injured.Often observe, inspect periodically,
It was found that disease, carries out rational treatment in time.Divide pond in good time：According to the growing state of fry, timely divider lattice, point pond is raised.
For the operations in embodiment 1, conscientiously observe, every record is carried out conscientiously.Wherein, artificial propagation test ties
Fruit is as shown in table 1；Fry rearing result of the test is as shown in table 2.
The artificial propagation test's result of table 1
The fry rearing result of the test of table 2
As a result show, spawning rate is that 89.12%, rate of fertilization is that 90.11%, incubation rate is that 88.32%, survival rate of fish fry is
More than 8%, it can concentrate, the neat mouth schizothoracin fry of acquisition of batch, to realize that the large-scale cultivation of neat mouth schizothoracin is provided reliably
Introduces a collection basis, align mouth schizothoracin wild resource protection and large-scale cultivation it is important great.
Claims (10)
1. a kind of artificial breeding method of neat mouth schizothoracin fry, it is characterised in that concretely comprise the following steps：
1）Parent fish rearing：Parent fish rearing pond is flowing water pond, and breeding density is 1-3 tails/m2, the nurturing period, determine matter, fixed point, timing,
Quantitatively fed；Determine matter：Feed crude protein content 32%~35%, calorie-protein ratio（E/P）36kJ/g~40kJ/g's is expanded
Pellet and/or water earthworm living bait；Fixed point：Feedstuff feeding, which should uniformly be thrown, is spread on the FX of fishpond one, when feeding every time
Between 25-35min；Regularly：Day feeds number of times and determined according to water temperature situation of change, and is relatively fixed the time；It is quantitative：Day feeding rate is
The 2~3% of fish body weight；
2）Parent population selects and combo：Select 4 more than age sexually matured neat schistostoma abdomen fish parent fish, physique healthy and strong, not damaged, by male and female
The combo of ratio 1.5: 1；
3）Artificial induced spawning：In neat mouth schizothoracin mating period, sexually matured parent population is selected, luteinizing hormone-releasing hormone is injected
Analog, is then put into spawning inducing pool by parent population, and ripe fish density is 1 group/m2, stimulation by running water is kept in pond；
4）Insemination：After hastening parturition, parent population active situation is observed, it is found that parent population has obvious estrus behavior, draws in the net to check parent population, gently presses female
There is ovum grain outflow in fish belly portion, using dry method of artificial fertilization；
5）Incubating oosperm：Incubator is positioned in hatching tank, and bottom is away from water surface 10-15cm, by the fertilization after artificial insemination
Ovum, which is laid in incubator, hatches, and Spawning water is through filtration treatment, and dissolved oxygen >=6.0 mg/L, pH be neutral or alkalescence, water temperature 15-
18℃；
6）Prelarva is temporarily supported：After incubating oosperm membrane, prelarva is put into hatching tank and temporarily supported, fish is transferred to after the flat trip of fry floating
Seedling culture pond；
7）Fry rearing：Micro- running water technology, fry rearing pond water velocity 1-2cm/s are taken, fry breeding density is 1500-
2000 tails/m2, open-mouthed bait is water earthworm, when fry body length reaches 2cm, progressively tames and dociles food with mixed feed；Residual bait is removed in time
And dirt, inspect periodically, it is found that disease is treated in time；
By above-mentioned steps, the artificial breeding of neat mouth schizothoracin fry is completed.
2. according to the method described in claim 1, it is characterised in that：The parent fish rearing pond and fry rearing pond are rectangular
Shape, one end sets water inlet, and the other end sets discharge outlet, and discharge outlet sets spilling water station pipe, and drainage mouthful is respectively provided with anti-escape set
Apply；Surface water inlet, the mode of bottom discharge is taken to carry out water body exchange.
3. according to the method described in claim 1, it is characterised in that：The water control in parent fish rearing pond is exchanging 5-8 daily
It is secondary, the mg/L of dissolved oxygen >=6.0, pH value is neutral or meta-alkalescence, transparency >=50cm；And bred first 2 months in parent population, timing is increased
Bath is stimulated, and flow velocity is 3-5cm/s.
4. according to the method described in claim 1, it is characterised in that：Step 4）Described in obvious estrus behavior be that heat is high
Tide；Mutually compel between proestrus group, female milter by being vied each other between milter；Middle and later periods, female milter is intermittently mutually with kiss
Answer back to other side's belly in portion；Later stage, female fish swimming is slow, waits milter, when female milter abuts side by side, raun body drastically trembles,
This is spawning sign, i.e. heat climax.
5. according to the method described in claim 1, it is characterised in that：Hatching tank is rectangular-shape, and one end sets water inlet, another
Hold bottom that osculum and drainage pipeline are set；The depth of water is maintained at 30-35cm, and incubator is wood frame, and bottom and surrounding are thin,tough silk
Screen cloth.
6. according to the method described in claim 1, it is characterised in that：Step 5）In hatching process, miniflow water, water flow velocity 1- are kept
Incubator is shaken in 2cm/s, timing, and rejects water mold ovum.
7. according to the method described in claim 1, it is characterised in that：The fry rearing pond locally sets shadow shield, prevents strong
Light direct beam, takes micro- running water technology, has abundant water resources, and water waterflow stabilization, water quality is pure and fresh；Results of regular determination physicochemical factors；Fish
Seedling cultivates early stage 1-2d suctions dirt 1 time, and middle and later periods, 3-5d suctions are dirty 1 time.
8. the method according to claim 1-7 any one, it is characterised in that：Parent fish rearing pond, spawning inducing pool, hatching water
Groove, incubator and fry rearing pond, the 2-4d before use soak 24h with 5ppm chlorine dioxide or 20ppm Betagen Solutions,
Come into operation after cleaning.
9. the method according to claim 1-7 any one, it is characterised in that：The depth of water in parent fish rearing pond is 120-
180cm, the depth of water of spawning inducing pool is maintained at the depth of water in 60-70cm, hatching tank and is maintained at 30-35cm, the depth of water in fry rearing pond
In 50-100cm.
10. according to the method described in claim 1, it is characterised in that：It is described hatching tank length, width and height size for 3.0m ×
0.8 m × 0.4m, the size of the length, width and height of incubator is to place 3-5 incubator in 0.6m × 0.4m × 0.2m, hatching tank.
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| CN107593530A (en) * | 2017-09-26 | 2018-01-19 | 西南大学 | Neat mouth schizothoracin is cultivated and propagation method |
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