CN106577389A - Artificial multiplication method of schizothorax prenati - Google Patents

Artificial multiplication method of schizothorax prenati Download PDF

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Publication number
CN106577389A
CN106577389A CN201611154977.4A CN201611154977A CN106577389A CN 106577389 A CN106577389 A CN 106577389A CN 201611154977 A CN201611154977 A CN 201611154977A CN 106577389 A CN106577389 A CN 106577389A
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parts
artificial
parent population
water
chinese medicine
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Inventor
周波
龙治海
李军
王泽仁
卢华
徐飞
戴绍祥
林玉良
冯波
田小青
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FISHERIES INSTITUTE SICHUAN ACADEMY OF AGRICULTURAL SCIENCES
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FISHERIES INSTITUTE SICHUAN ACADEMY OF AGRICULTURAL SCIENCES
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Publication of CN106577389A publication Critical patent/CN106577389A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

Abstract

The invention discloses an artificial multiplication method of schizothorax prenati. The method comprises the four steps of selecting and culturing parent fishes, artificial induced spawning, artificial inseminating and incubating. The method is low in cost, high in induced spawning efficiency, high in survival rate and fast in growth; specific oxytocic is adopted to be injected, so that the induced spawning efficiency is greatly improved; and meanwhile, an artificial compound feed containing a specific formula content is adopted to feed the schizothorax prenati, so that the growth velocity is greatly improved, meanwhile, the immunity and the resistance of the schizothorax are effectively improved, the survival rate is greatly improved, and the artificial multiplication method of the schizothorax prenati is a very effective multiplication method.

Description

A kind of artificial fecundation method of neat mouth schizothoracin
Technical field
Present invention relates particularly to a kind of artificial fecundation method of neat mouth schizothoracin.
Background technology
Neat mouth schizothoracin is called refined fish, Qi Kou, thin soft-shelled turtle, neat mouth brachymystax lenok, belongs to cold water fishes, is demersal fishes, Relatively low temperature environment is sought, likes moving in anxious unhurried current intersection, at ordinary times in a small bay in a rivers for moving in unhurried current, season of ingesting is in substrate more For activity in husky and gravel, the environment that The turbulent river crashes its way through, there is short-range breeding migration phenomenon, female needed for 4 ages reached sexal maturity, male It is general to reach sexal maturity in 3 ages.Neat mouth schizothoracin is distributed in the water systems such as Ming River, Dadu River, is a kind of important edible of Upper Yangtze River Fish, individual big, generally 0.5-1.0kg, maximum can reach 4.0-5.0kg.Because its meat is fertile, rich in fat, people is received deeply Like, it is serious due to illegally fishing in recent years, cause wild neat mouth schizothoracin to reduce increasingly, existing market price has reached 160-200 Unit/kg, seed price is 0.8-1 units/cm, and market is very in short supply, and endangered at present, the neat mouth schizothoracin of artificial propagation is Become a kind of inevitable.Although schizothoracin there are some to report, the technology phase that neat mouth schizothoracin is propagated artificially is specific to To little.
The artificial fecundation method of existing neat mouth schizothoracin mainly discloses the training in the stages such as insemination, hatching, nursery cultivation Condition, such as temperature, time and environmental requirement etc. are educated, the feed for feeding only is the biological bait such as water earthworm, yellow meal worm, these Although bait can provide some nutriments, nutrition is not comprehensive;Meanwhile, existing neat mouth schizothoracin artificial propagation techniques Spawning rate, fertility rate and hatchability are relatively low, and extremely unstable, leverage the development of neat mouth schizothoracin industry.
The content of the invention
The technical problem to be solved in the present invention is:It is directed to deficiency of the prior art, there is provided a kind of neat mouth schizothoracin Artificial fecundation method, the method is effectively improved cultivates the ripe quality and quantity of parent population, substantially increases hastening parturition for parent population Rate and the incubation rate of embryonated egg, while the recovery time of postpartum parent population can be shortened, improve growth rate and the immunity of parent population Ability.
The technical scheme that the present invention solves above-mentioned technical problem is as follows:A kind of artificial propagation side of neat mouth schizothoracin is provided Method, including:
(One)Parent population selects and cultivates:The parent population of the age of milter 4, raun 5 age above body weight more than 2kg is selected to be invested in nursery cultivation Chi Zhong;Water body environment is:More than dissolved oxygen 6.0mg/L, flow rate of water flow 1m/h~1.5m/h, transparency >=40cm, pH value 7.0 ~7.5,4~22 DEG C of water temperature;
Cultivate pool area 20-40m2, depth of water 1.0-1.5m;
Using flowing water culture pattern, cultivation density is 1.0kg/m3~3kg/m3
Neat mouth schizothoracin enters behind pond, feeds artifical compound feed, feeding volume for parent population body weight 1.0%-1.5%, the daily morning and Respectively feed once in the afternoon;
(Two)Artificial induced spawning:The good neat mouth schizothoracin raun of physically fit, gonad development and milter are selected as parent population of hastening parturition, The individual ratio of raun and milter is 2-3:1;
In the annual 3-4 months to hasten parturition period, water temperature of hastening parturition is 10-13 DEG C;
Oxytocic drug is injected to parent population, specially:For raun, injected at twice using intraperitoneal injection, noted for the first time After the amount of penetrating is for 30%, the 20-24 hours of accumulated dose, second injection is carried out, injection volume is remaining 70%;For milter, carry out A shot, second injection time of injection time and raun is synchronous;
Female parent population oxytocic drug dosage is:40 μ gLRH-A2/kg body weight+80mgDOM/kg body weight, male parent population oxytocic drug Dosage is the 1/2 of above-mentioned female parent population;
(Three)Artificial insemination:After oxytocic drug has been injected, effect 40-60 hour, using semidry method artificial insemination, is fertilized Ovum, completes artificial induced spawning;
(Four)Hatching:By embryonated egg be placed in 12~17 DEG C of water temperature, the depth of water for 50cm, dissolved oxygen be more than 6mg/L, flow velocity be 2.0 Hatching 3~4 days in the Vickers incubator of~2.5m/s, are then down to flow velocity 1.0~1.5m/s and hatch 2~3 days, and adopt 80~100 mesh screen cloths are filtered to keep water quality pure and fresh, and after 130~168h, hatching is emerged.
In order to reach more preferable effect, the present invention can also do following improvement:
The artifical compound feed is made up of the component of following weight portion:10 parts of fish meal, 20 parts of peanut cake, 2 parts of bone meal, micro unit 3 parts of 3 parts of plain premix, 2 parts of dusty yeast, 5 parts of bacillus subtilis powder, 30 parts of dregs of beans, 3 parts of dried silkworm chrysalis meal and Chinese medicine additive;
The Chinese medicine additive is made up of the raw material of following weight portion:20 parts of perillaseed, 30 parts of gingko, 3 parts of blackspiked lovegrass herb with root, Radix Glycyrrhizae 12 1 part of part, 10 parts of the membrane of a chicken's gizzard, 5 parts of cloves and cordate houttuynia.
Further, the preparation method of above-mentioned artifical compound feed is:Each component is weighed according to formula rate, then by each group 20-30 mesh sieves are crushed in point input pulverizer, remix uniform, then put in granulator, at 60-70 DEG C particle is made, Obtain.
Further, the preparation method of above-mentioned Chinese medicine additive is:Each component is weighed according to formula rate, by Chinese medicine additive With water according to parts by weight 40:90 add water to cook, and decoct three times, 10 minutes every time, filter, and powder is made in concentration, drying, obtains Chinese medicine additive.
In the feed formula of the present invention:
The gross protein value of bone meal is between 50-60%, and lysine content is high, and vitamin B complex is more, and bone meal contains in a large number Calcium, phosphorus and manganese, wherein, phosphorus is the absorbing beneficial to fish using phosphorus, and the growth and breeding of fish are stimulated to some extent.
Microelement pre-mix material provides enough mineral matters rich in abundant iron, zinc and selenium, the growth for fish so as to raw It is long rapid.
The pharmacological property of above-mentioned Chinese medicinal ingredients is:
Perillaseed:It is pungent, temperature, return lung, large intestine channel;Sending down abnormally ascending dissolving phlegm, it is relieving cough and asthma, relax bowel;
Gingko:Determine asthma due to excessive phlegm, check with turbidity, antidiarrheal to let out, detoxify, reduce just;Containing various nutrient elements, isolating protein, fat, sugar Class, also containing trace elements such as vitamin C, vitamin B2, carrotene, calcium, phosphorus, iron, potassium and magnesium, and ginkgoic acid, bilobol With the composition such as polysaccharide.
Cloves:Pungent, the taste temperature of property;Enter lung, spleen, stomach, the Jing of kidney four;Effect:Middle benefit gas, warm kidney, drop are inverse.
Cordate houttuynia:It is cool in nature, nontoxic, return lung Jing, bladder, large intestine channel;Cure mainly:The hot cough and asthma of phlegm;Throat;Hot dysentery;Carbuncle sore tumefacting virus; Heat gonorrhea.
The membrane of a chicken's gizzard:It is sweet, tremble with fear;Returns spleen, stomach, small intestine, bladder warp, food digesting stomach fortifying is aid digestion.
The artifical compound feed of the present invention greatly improves exempting from for fish in the case where effectively facilitating the normal growth of fish Epidemic disease resistance against diseases, promotes neat mouth schizothoracin fast-growth, is that neat mouth schizothoracin keeps good condition of health and optimal growth Enough nutritional needs are provided.
The Chinese herb feed additive of the present invention, can adjust the allomeric function of neat mouth schizothoracin, improve the disease-resistant of body itself Ability, and then the immunocompetence of fish is improved, promote it to grow, also, the Chinese medicine additive Small side effects, resistance will not be produced Property, it is safe and reliable.
The invention has the advantages that:
The artificial fecundation method of the neat mouth schizothoracin of the present invention is selected and cultivation, artificial induced spawning, artificial insemination and received using parent population Four steps of smart egg hatching, low cost, efficiency high of hastening parturition, survival rate is high, grows soon, and employ specific oxytocic drug Row injection, significantly there is provided efficiency of hastening parturition, while using the alignment mouth schizothoracin of the artifical compound feed with special formulation content Raised, while its growth rate is greatly improved, be also effectively improved the immunity and resistance of schizothoracin, significantly Its survival rate is improved, is a kind of highly effective propagation method.
The present invention has the oxytocic drug of specific components and content by preparing, while neat mouth schizothoracin is not injured, Also effectively facilitate the gonad development of male and female parent population completely, while making the heat simultaneously of most of parent populations, improve the efficiency of artificial induced spawning, It is more beneficial for advanced fry cultivation and management work.
By using method for incubating oosperm such as specific water temperature, flow controls, substantially increasing incubating for neat mouth schizothoracin Rate and survival rate, are that the large scale cultivating of neat mouth schizothoracin is had laid a good foundation.
Specific embodiment
Hereinafter describe the present invention, example is served only for explaining the present invention, is not intended to limit the present invention Scope.
Embodiment 1:
The artificial fecundation method of neat mouth schizothoracin, including:
(One)Parent fish rearing:The parent population that the age of milter 4, raun 5 are more than age and body weight is more than 2kg is selected to be invested in nursery culture pond In;Water body environment is:More than dissolved oxygen 6.0mg/L, flow rate of water flow 1m/h, transparency >=40cm, pH value 7.0 ~ 7.5, water temperature 4- 22℃;
Cultivate pool area 20m2, depth of water 1.0m;
Using flowing water culture pattern, cultivation density is 2.0kg/m3
Neat mouth schizothoracin enters behind pond, feeds artifical compound feed, and feeding volume is the 1.5% of parent population body weight, and the daily morning and afternoon are each Feed once;
Artifical compound feed is made up of the component of following weight portion:
10 parts of fish meal, 20 parts of peanut cake, 2 parts of bone meal, 3 parts of microelement pre-mix material, 2 parts of dusty yeast, bacillus subtilis powder 5 Part, 30 parts of dregs of beans, 3 parts of dried silkworm chrysalis meal and 3 parts of Chinese medicine additive;
The preparation method of above-mentioned artifical compound feed is:Each component is weighed according to formula rate, then crushes each component input 20-30 mesh sieves are crushed in machine, are remixed uniformly, then put in granulator, at 60-70 DEG C particle is made, obtained.
Chinese medicine additive is made up of the raw material of following weight portion:20 parts of perillaseed, 30 parts of gingko, 3 parts of blackspiked lovegrass herb with root, Radix Glycyrrhizae 12 1 part of part, 10 parts of the membrane of a chicken's gizzard, 5 parts of cloves and cordate houttuynia;
The preparation method of above-mentioned Chinese medicine additive is:Each component is weighed according to formula rate, by Chinese medicine additive and water according to weight Amount number 40:90 add water to cook, and decoct three times, 10 minutes every time, filter, and powder is made in concentration, drying, obtains Chinese medicine addition Agent.
(Two)Artificial induced spawning:The good neat mouth schizothoracin raun of physically fit, gonad development and milter are selected as hastening parturition The individual ratio of parent population, raun and milter is 2:1;
In the annual 3-4 months to hasten parturition period, water temperature of hastening parturition is 10 DEG C;
Oxytocic drug is injected to parent population, specially:For raun, injected at twice using intraperitoneal injection, noted for the first time The amount of penetrating is the 30% of accumulated dose, after 22 hours, carries out second injection, and injection volume is remaining 70%;For milter, carry out once Injection, second injection time of injection time and raun is synchronous;
Female parent population oxytocic drug dosage is:40 μ gLRH-A2/kg body weight+80mgDOM/kg body weight, male parent population oxytocic drug Dosage is the 1/2 of above-mentioned female parent population;
(Three)Artificial insemination:After oxytocic drug has been injected, effect 60 hours, using semidry method artificial insemination, obtains embryonated egg, Complete artificial induced spawning;
(Four)Hatching:It is more than 8mg/L, flow velocity for 2.5m/s that embryonated egg is placed in into 13 DEG C of water temperature, the depth of water for 50cm, dissolved oxygen Hatching 3~4 days in Vickers incubator, are then down to flow velocity 1.0m/s and hatch 2~3 days, and using 80~100 mesh screen cloth mistakes To keep water quality pure and fresh, after 160h, hatching is emerged for filter.
Embodiment 2
The artificial fecundation method of neat mouth schizothoracin, including:
(One)Parent fish rearing:The parent population that the age of milter 4, raun 5 are more than age and body weight is more than 2kg is selected to be invested in nursery culture pond In;Water body environment is:More than dissolved oxygen 6.0mg/L, flow rate of water flow 1.5m/h, transparency >=40cm, pH value 7.0 ~ 7.5, water temperature 4-22℃;
Cultivate pool area 40m2, depth of water 1.5m;
Using flowing water culture pattern, cultivation density is 3.0kg/m3
Neat mouth schizothoracin enters behind pond, feeds artifical compound feed, and feeding volume is the 1.0% of parent population body weight, and the daily morning and afternoon are each Feed once;
Artifical compound feed is made up of the component of following weight portion:
20 parts of fish meal, 10 parts of peanut cake, 3 parts of bone meal, 2 parts of microelement pre-mix material, 3 parts of dusty yeast, bacillus subtilis powder 3 Part, 20 parts of dregs of beans, 2 parts of dried silkworm chrysalis meal and 5 parts of Chinese medicine additive;
The preparation method of above-mentioned artifical compound feed is:Each component is weighed according to formula rate, then crushes each component input 20-30 mesh sieves are crushed in machine, are remixed uniformly, then put in granulator, at 60-70 DEG C particle is made, obtained.
Chinese medicine additive is made up of the raw material of following weight portion:30 parts of perillaseed, 20 parts of gingko, 5 parts of blackspiked lovegrass herb with root, Radix Glycyrrhizae 10 2 parts of part, 20 parts of the membrane of a chicken's gizzard, 7 parts of cloves and cordate houttuynia;
The preparation method of above-mentioned Chinese medicine additive is:Each component is weighed according to formula rate, by Chinese medicine additive and water according to weight Amount number 40:90 add water to cook, and decoct three times, 10 minutes every time, filter, and powder is made in concentration, drying, obtains Chinese medicine addition Agent.
(Two)Artificial induced spawning:The good neat mouth schizothoracin raun of physically fit, gonad development and milter are selected as hastening parturition The individual ratio of parent population, raun and milter is 3:1;
In the annual 3-4 months to hasten parturition period, water temperature of hastening parturition is 10 DEG C;
Oxytocic drug is injected to parent population, specially:For raun, injected at twice using intraperitoneal injection, noted for the first time The amount of penetrating is the 30% of accumulated dose, after 20 hours, carries out second injection, and injection volume is remaining 70%;For milter, carry out once Injection, second injection time of injection time and raun is synchronous;
Female parent population oxytocic drug dosage is:40 μ g LRH-A2/kg body weight+80mgDOM/kg body weight, male parent population oxytocic drug Dosage is the 1/2 of above-mentioned female parent population;
(Three)Artificial insemination:After oxytocic drug has been injected, effect 44 hours, using semidry method artificial insemination, obtains embryonated egg, Complete artificial induced spawning;
(Four)Hatching:It is 50cm, the Vickers that dissolved oxygen is 6mg/L, flow velocity is 2.0m/s that embryonated egg is placed in into 17 DEG C of water temperature, the depth of water Hatching 3 ~ 4 days in incubator, are then down to flow velocity 1.5m/s and hatch 2~3 days, and adopt 80-100 mesh screen cloth to filter to protect Water holding matter is pure and fresh, and after 138h, hatching is emerged.
Embodiment 3
The artificial fecundation method of neat mouth schizothoracin, including:
(One)Parent fish rearing:The parent population that the age of milter 4, raun 5 are more than age and body weight is more than 2kg is selected to be invested in nursery culture pond In;Water body environment is:More than dissolved oxygen 6.0mg/L, flow rate of water flow 1.2m/h, transparency >=40cm, pH value 7.0 ~ 7.5, water temperature 4-22℃;
Take up an area 30m in fry rearing pond2, depth of water 1.5m;
Flowing water culture, cultivation density is 2.0kg/m3
Neat mouth schizothoracin enters behind pond, feeds artifical compound feed, and feeding volume is the 1.0% of parent population body weight, and the daily morning and afternoon are each Feed once;
Artifical compound feed is made up of the component of following weight portion:
15 parts of fish meal, 15 parts of peanut cake, 2 parts of bone meal, 2 parts of microelement pre-mix material, 3 parts of dusty yeast, bacillus subtilis powder 4 Part, 25 parts of dregs of beans, 3 parts of dried silkworm chrysalis meal and 4 parts of Chinese medicine additive;
Chinese medicine additive is made up of the raw material of following weight portion:25 parts of perillaseed, 25 parts of gingko, 4 parts of blackspiked lovegrass herb with root, 11 parts of Radix Glycyrrhizae, chicken 1 part of interior golden 15 parts, 6 parts of cloves and cordate houttuynia.
(Two)Artificial induced spawning:The good neat mouth schizothoracin raun of physically fit, gonad development and milter are selected as hastening parturition The individual ratio of parent population, raun and milter is 3:1;
In the annual 3-4 months to hasten parturition period, water temperature of hastening parturition is 12 DEG C;
Oxytocic drug is injected to parent population, specially:For raun, injected at twice using intraperitoneal injection, noted for the first time The amount of penetrating is the 30% of accumulated dose, after 24 hours, carries out second injection, and injection volume is remaining 70%;For milter, carry out once Injection, second injection time of injection time and raun is synchronous;
Female parent population oxytocic drug dosage is:40 μ g LRH-A2/kg body weight+80mgDOM/kg body weight, male parent population oxytocic drug Dosage is the 1/2 of above-mentioned female parent population;
(Three)Artificial insemination:After oxytocic drug has been injected, effect 50 hours, using semidry method artificial insemination, obtains embryonated egg, Complete artificial induced spawning;
(Four)Hatching:It is 50cm, the Vickers that dissolved oxygen is 6mg/L, flow velocity is 2.3m/s that embryonated egg is placed in into 16 DEG C of water temperature, the depth of water Hatching 3 ~ 4 days in incubator, are then down to flow velocity 1.2m/s and hatch 2~3 days, and adopt 80-100 mesh screen cloth to filter to protect Water holding matter is pure and fresh, and after 144h, hatching is emerged.
It is not to this although being described in detail to the specific embodiment of the present invention in conjunction with specific embodiments The restriction of scope of patent protection.In claims limited range, those skilled in the art is without creative work The various modifications or adjustment that can be made still are protected by this patent.

Claims (5)

1. a kind of artificial fecundation method of neat mouth schizothoracin, is characterized in that, including:
(One)Parent population selects and cultivates:The parent population that the age of milter 4, raun 5 are more than age and body weight is more than 2kg is selected to be invested in nursery training In educating pond;Nurturing an environment:More than dissolved oxygen 6.0mg/L, flow rate of water flow 1m/h~1.5m/h, transparency >=40cm, pH value 7.0 ~7.5,4~22 DEG C of water temperature;
Cultivate pool area 20-40m2, depth of water 1.0-1.5m;
Using flowing water culture pattern, cultivation density is 1.0kg/m3~3kg/m3
Neat mouth schizothoracin enters behind pond, feeds artifical compound feed, feeding volume for parent population body weight 1.0%-1.5%, the daily morning and Respectively feed once in the afternoon;
(Two)Artificial induced spawning:The good neat mouth schizothoracin raun of physically fit, gonad development and milter are selected as parent population of hastening parturition, The individual ratio of raun and milter is 2-3:1;
In the annual 3-4 months to hasten parturition period, water temperature of hastening parturition is 10-13 DEG C;
Oxytocic drug is injected to parent population, specially:For raun, injected at twice using intraperitoneal injection, noted for the first time After the amount of penetrating is for 30%, the 20-24 hours of accumulated dose, second injection is carried out, injection volume is remaining 70%;For milter, carry out A shot, second injection time of injection time and raun is synchronous;
Female parent population oxytocic drug dosage is:40 μ g LRH-A2/kg body weight+80mg DOM/kg body weight, male parent population oxytocic Agent amount is the 1/2 of above-mentioned female parent population;
(Three)Artificial insemination:After oxytocic drug has been injected, effect 40-60 hour, using semidry method artificial insemination, is fertilized Ovum, completes artificial induced spawning;
(Four)Hatching:By embryonated egg be placed in 12~17 DEG C of water temperature, the depth of water for 50cm, dissolved oxygen be more than 6mg/L, flow velocity be 2.0 Hatching 3~4 days in the Vickers incubator of~2.5m/s, are then down to flow velocity 1.0~1.5m/s and hatch 2~3 days, and adopt 80~100 mesh screen cloths are filtered to keep water quality pure and fresh, and after 130~168h, hatching is emerged.
2. the artificial fecundation method of neat mouth schizothoracin according to claim 1, is characterized in that, the artifical compound feed by The component composition of following weight portion:
Fish meal 10-20 parts, peanut cake 10-20 parts, bone meal 2-3 parts, microelement pre-mix material 2-3 parts, dusty yeast 2-3 parts, withered grass Bacillus powder 3-5 parts, dregs of beans 20-30 parts, dried silkworm chrysalis meal 2-3 parts and Chinese medicine additive 3-5 parts;
The Chinese medicine additive is made up of the raw material of following weight portion:Perillaseed 20-30 part, gingko 20-30 parts, blackspiked lovegrass herb with root 3-5 parts, Radix Glycyrrhizae 10-12 parts, the membrane of a chicken's gizzard 10-20 parts, cloves 5-7 parts and cordate houttuynia 1-2 parts.
3. the artificial fecundation method of neat mouth schizothoracin according to claim 1, is characterized in that, the artifical compound feed by The component composition of following weight portion:
10 parts of fish meal, 20 parts of peanut cake, 2 parts of bone meal, 3 parts of microelement pre-mix material, 2 parts of dusty yeast, bacillus subtilis powder 5 Part, 30 parts of dregs of beans, 3 parts of dried silkworm chrysalis meal and 3 parts of Chinese medicine additive;
The Chinese medicine additive is made up of the raw material of following weight portion:20 parts of perillaseed, 30 parts of gingko, 3 parts of blackspiked lovegrass herb with root, Radix Glycyrrhizae 12 1 part of part, 10 parts of the membrane of a chicken's gizzard, 5 parts of cloves and cordate houttuynia.
4. the artificial fecundation method of the neat mouth schizothoracin according to Claims 2 or 3, is characterized in that, above-mentioned hand fit raises The preparation method of material is:Each component is weighed according to formula rate, then each component is put in pulverizer and is crushed to 20-30 mesh Sieve, remixes uniformly, then puts in granulator, and at 60-70 DEG C particle is made, and obtains.
5. the artificial fecundation method of the neat mouth schizothoracin according to Claims 2 or 3, is characterized in that, above-mentioned Chinese medicine additive Preparation method be:Each component is weighed according to formula rate, by Chinese medicine additive and water according to parts by weight 40:90 add decocting Boil, decoct three times, 10 minutes every time, filter, powder is made in concentration, drying, obtains Chinese medicine additive.
CN201611154977.4A 2016-12-14 2016-12-14 Artificial multiplication method of schizothorax prenati Pending CN106577389A (en)

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CN107135989A (en) * 2017-05-02 2017-09-08 宜昌市水产技术推广站 A kind of artificial breeding method of neat mouth schizothoracin fry
CN107593530A (en) * 2017-09-26 2018-01-19 西南大学 Neat mouth schizothoracin is cultivated and propagation method
CN109258522A (en) * 2018-10-08 2019-01-25 王雪霁 A kind of inexpensive high yield environment-protecting cultivation method of water silver carp
CN109287528A (en) * 2018-10-08 2019-02-01 王纪芝 A kind of ecology bighead solid interaction cultural method
CN114403057A (en) * 2022-02-14 2022-04-29 四川省农业科学院水产研究所 Leiocassis longirostris breeding method

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107135989A (en) * 2017-05-02 2017-09-08 宜昌市水产技术推广站 A kind of artificial breeding method of neat mouth schizothoracin fry
CN107593530A (en) * 2017-09-26 2018-01-19 西南大学 Neat mouth schizothoracin is cultivated and propagation method
CN109258522A (en) * 2018-10-08 2019-01-25 王雪霁 A kind of inexpensive high yield environment-protecting cultivation method of water silver carp
CN109287528A (en) * 2018-10-08 2019-02-01 王纪芝 A kind of ecology bighead solid interaction cultural method
CN114403057A (en) * 2022-02-14 2022-04-29 四川省农业科学院水产研究所 Leiocassis longirostris breeding method

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