MXPA02009895A - Proteinas de fusion de antigeno de nucleo de hepatitis b. - Google Patents

Proteinas de fusion de antigeno de nucleo de hepatitis b.

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Publication number
MXPA02009895A
MXPA02009895A MXPA02009895A MXPA02009895A MXPA02009895A MX PA02009895 A MXPA02009895 A MX PA02009895A MX PA02009895 A MXPA02009895 A MX PA02009895A MX PA02009895 A MXPA02009895 A MX PA02009895A MX PA02009895 A MXPA02009895 A MX PA02009895A
Authority
MX
Mexico
Prior art keywords
particles
loop
hbcag
sequences
core
Prior art date
Application number
MXPA02009895A
Other languages
English (en)
Inventor
David Rowlands
Original Assignee
Univ Leeds Innovations Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Leeds Innovations Ltd filed Critical Univ Leeds Innovations Ltd
Publication of MXPA02009895A publication Critical patent/MXPA02009895A/es

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/01—DNA viruses
    • C07K14/02—Hepadnaviridae, e.g. hepatitis B virus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/20—Antivirals for DNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/53—DNA (RNA) vaccination
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2730/00—Reverse transcribing DNA viruses
    • C12N2730/00011—Details
    • C12N2730/10011—Hepadnaviridae
    • C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
    • C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Wood Science & Technology (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Communicable Diseases (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Oncology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

La capsida del virus de hepatitis B (HBV) se elabora de una especie individual de proteina llamada el antigeno de nucleo (HBcAg) que se auto-ensambla en particulas. Las particulas son altamente inmunogenicas y son capaces de presentar epitopes heterologos al sistema inmunitario cuando los epitopes se insertan en una region expuesta en la superficie de las particulas llamada la "asa el". Los bloques de construccion estructural de las particulas son dimeros estrechamente asociados de HBcAg en los cuales las asas el adyacentes estan yuxtapuestas de forma cercana. Se propone que las secuencias insertadas en la asa el, se restrinjan conformacionalmente en las particulas ensambladas cuando se presentan en la proteina de nucleo monomerica. La invencion busca solucionar este problema al enlazar covalentemente proteinas de nucleo como copias en tandem, por ejemplo, como dimeros, de modo que se pueden hacer inserciones independientes en cada copia. Esto es particularmente util para la insercion de grandes secuencias en la asa el, debido a que permite que estas secuencias se inserten en solo una copia de la proteina de nucleo por repeticion en tandem, reduciendo de este modo las potenciales oposiciones conformacionales en el ensamble. De manera alternativa, se puede insertar una secuencia diferente en cada asa el de una repeticion en tandem, incrementando de esta manera la flexibilidad de las particulas de HBcAg como un sistema de distribucion de epitopes.
MXPA02009895A 2000-04-07 2001-04-09 Proteinas de fusion de antigeno de nucleo de hepatitis b. MXPA02009895A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP00107118 2000-04-07
PCT/GB2001/001607 WO2001077158A1 (en) 2000-04-07 2001-04-09 Hepatitis b core antigen fusion proteins

Publications (1)

Publication Number Publication Date
MXPA02009895A true MXPA02009895A (es) 2004-09-06

Family

ID=8168351

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA02009895A MXPA02009895A (es) 2000-04-07 2001-04-09 Proteinas de fusion de antigeno de nucleo de hepatitis b.

Country Status (18)

Country Link
US (1) US7270821B2 (es)
EP (1) EP1268530B1 (es)
JP (1) JP4713809B2 (es)
KR (1) KR100808313B1 (es)
CN (1) CN100360556C (es)
AP (1) AP1836A (es)
AT (1) ATE336502T1 (es)
AU (1) AU784345B2 (es)
BR (1) BR0109919A (es)
CA (1) CA2405438C (es)
DE (1) DE60122300T2 (es)
DK (1) DK1268530T3 (es)
EA (1) EA200201077A1 (es)
ES (1) ES2269371T3 (es)
MX (1) MXPA02009895A (es)
PT (1) PT1268530E (es)
WO (1) WO2001077158A1 (es)
ZA (1) ZA200208967B (es)

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DE60027581T2 (de) * 1999-10-08 2007-05-10 Celltech Pharma Europe Ltd., Slough Design von immunogene
US7128911B2 (en) 2001-01-19 2006-10-31 Cytos Biotechnology Ag Antigen arrays for treatment of bone disease
US7094409B2 (en) 2001-01-19 2006-08-22 Cytos Biotechnology Ag Antigen arrays for treatment of allergic eosinophilic diseases
WO2003024481A2 (en) 2001-09-14 2003-03-27 Cytos Biotechnology Ag Packaging of immunostimulatory substances into virus-like particles: method of preparation and use
KR100465643B1 (ko) * 2001-09-28 2005-01-13 (주) 에이프로젠 비형 간염 바이러스 프리에쓰1 항원 유래의 에피토프 태그및 이에 대한 항체를 이용한 폴리펩티드의 검출 및 정제방법
US7115266B2 (en) 2001-10-05 2006-10-03 Cytos Biotechnology Ag Angiotensin peptide-carrier conjugates and uses thereof
BR0213117A (pt) 2001-10-05 2004-09-21 Cytos Biotechnology Ag Conjugados peptìdeo angiotensina-veìculo e seus usos
IN2008CN05409A (es) 2002-07-18 2015-10-02 Cytos Biotechnology Ag
WO2004016282A1 (en) 2002-07-19 2004-02-26 Cytos Biotechnology Ag Vaccine compositions containing amyloid beta1-6 antigen arrays
US7537767B2 (en) 2003-03-26 2009-05-26 Cytis Biotechnology Ag Melan-A- carrier conjugates
EP1606398A1 (en) 2003-03-26 2005-12-21 Cytos Biotechnology AG Melan-a peptide analogue-virus-like-particle conjugates
CN100537762C (zh) * 2004-06-24 2009-09-09 中国人民解放军军事医学科学院野战输血研究所 一种嵌合型病毒样颗粒疫苗载体蛋白及其制备方法与应用
KR100687653B1 (ko) * 2005-05-13 2007-02-27 유기현 스타-델타 결선용 전자개폐기의 제어방법
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NZ598356A (en) 2009-07-30 2014-06-27 Pfizer Vaccines Llc Antigenic tau peptides and uses thereof
MX2012002639A (es) 2009-09-03 2012-03-14 Pfizer Vaccines Llc Vacuna de pcsk9.
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RU2677799C2 (ru) * 2012-12-11 2019-01-21 Озивакс Сас Модифицированные суперспиральные белки с улучшенными свойствами
JP2016528176A (ja) * 2013-06-06 2016-09-15 ジェイ ローランズ、デビッド シングルドメイン抗体ディスプレイ
CN104341506A (zh) * 2013-07-30 2015-02-11 复旦大学 一种重组融合蛋白及其用途
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Also Published As

Publication number Publication date
ATE336502T1 (de) 2006-09-15
KR20030009429A (ko) 2003-01-29
DK1268530T3 (da) 2006-11-13
JP2003530123A (ja) 2003-10-14
DE60122300D1 (de) 2006-09-28
PT1268530E (pt) 2006-12-29
ES2269371T3 (es) 2007-04-01
DE60122300T2 (de) 2007-08-30
EP1268530A1 (en) 2003-01-02
JP4713809B2 (ja) 2011-06-29
WO2001077158A1 (en) 2001-10-18
EA200201077A1 (ru) 2003-04-24
AU4670601A (en) 2001-10-23
CA2405438A1 (en) 2001-10-18
AP2002002658A0 (en) 2002-12-31
CN1441807A (zh) 2003-09-10
AP1836A (en) 2008-03-14
US20040223965A1 (en) 2004-11-11
US7270821B2 (en) 2007-09-18
AU784345B2 (en) 2006-03-16
KR100808313B1 (ko) 2008-02-27
BR0109919A (pt) 2003-03-11
ZA200208967B (en) 2003-10-08
EP1268530B1 (en) 2006-08-16
CA2405438C (en) 2011-07-12
CN100360556C (zh) 2008-01-09

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