MX9703244A - Expresion de lactoferrina recombinante procesada yfragmentos del polipeptido lactoferrina a partir de un producto de fusion en aspergillus. - Google Patents

Expresion de lactoferrina recombinante procesada yfragmentos del polipeptido lactoferrina a partir de un producto de fusion en aspergillus.

Info

Publication number
MX9703244A
MX9703244A MX9703244A MX9703244A MX9703244A MX 9703244 A MX9703244 A MX 9703244A MX 9703244 A MX9703244 A MX 9703244A MX 9703244 A MX9703244 A MX 9703244A MX 9703244 A MX9703244 A MX 9703244A
Authority
MX
Mexico
Prior art keywords
lactoferrin
aspergillus
polypeptide
ragments
expression
Prior art date
Application number
MX9703244A
Other languages
English (en)
Spanish (es)
Inventor
Orla M Conneely
Denis R Headon
Bert W O'malley
Original Assignee
Agennix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agennix Inc filed Critical Agennix Inc
Publication of MX9703244A publication Critical patent/MX9703244A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/79—Transferrins, e.g. lactoferrins, ovotransferrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Cosmetics (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
MX9703244A 1994-11-02 1997-05-02 Expresion de lactoferrina recombinante procesada yfragmentos del polipeptido lactoferrina a partir de un producto de fusion en aspergillus. MX9703244A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/303,009 US5571697A (en) 1989-05-05 1994-11-02 Expression of processed recombinant lactoferrin and lactoferrin polypeptide fragments from a fusion product in Aspergillus

Publications (1)

Publication Number Publication Date
MX9703244A true MX9703244A (es) 1998-07-31

Family

ID=23170177

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9703244A MX9703244A (es) 1994-11-02 1997-05-02 Expresion de lactoferrina recombinante procesada yfragmentos del polipeptido lactoferrina a partir de un producto de fusion en aspergillus.

Country Status (11)

Country Link
US (3) US5571697A (OSRAM)
EP (1) EP0871724A1 (OSRAM)
JP (1) JPH10509317A (OSRAM)
KR (1) KR100443307B1 (OSRAM)
CN (1) CN1230537C (OSRAM)
AU (1) AU4279896A (OSRAM)
CA (1) CA2204246A1 (OSRAM)
DE (1) DE871724T1 (OSRAM)
ES (1) ES2127164T1 (OSRAM)
MX (1) MX9703244A (OSRAM)
WO (1) WO1996014413A1 (OSRAM)

Families Citing this family (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5571697A (en) * 1989-05-05 1996-11-05 Baylor College Of Medicine Texas Medical Center Expression of processed recombinant lactoferrin and lactoferrin polypeptide fragments from a fusion product in Aspergillus
IL94183A (en) * 1989-05-05 2003-09-17 Baylor College Medicine cDNA SEQUENCE CODING FOR HUMAN LACTOFERRIN PROTEIN OR PORTION THEREOF AND LACTOFERRIN PROTEIN PRODUCED FROM SAID SEQUENCE
US6066469A (en) * 1990-03-08 2000-05-23 Ferro Dynamics, Inc. Cloning, expression, and uses of human lactoferrin
AT404838B (de) * 1995-11-24 1999-03-25 Immuno Ag Herstellung von proteinen aus pro-proteinen durch fusionsproteine abgeleitet von furin oder furinanalogen
US6111081A (en) * 1996-05-31 2000-08-29 Baylor College Of Medicine Lactoferrin variants and uses thereof
US5811381A (en) * 1996-10-10 1998-09-22 Mark A. Emalfarb Cellulase compositions and methods of use
US7883872B2 (en) * 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
AU5571699A (en) * 1998-08-19 2000-03-14 Monsanto Company An antifungal protein from tall fescue and its use in plant disease control
US6592814B2 (en) * 1998-10-02 2003-07-15 Johnson & Johnson Vision Care, Inc. Biomedical devices with antimicrobial coatings
DE69922978T2 (de) * 1998-10-06 2005-12-08 Emalfarb, Mark Aaron, Jupiter Transformationsystem in filamentösen fungiziden chrysosporium-wirtszellen
US6399570B1 (en) 1999-02-05 2002-06-04 Agennix, Inc. Antimicrobial/endotoxin neutralizing polypeptide
US7138150B2 (en) * 2000-05-02 2006-11-21 Ventria Bioscience Method of making an anti-infective composition for treating oral infections
US7417178B2 (en) * 2000-05-02 2008-08-26 Ventria Bioscience Expression of human milk proteins in transgenic plants
US20080050503A1 (en) * 2000-05-02 2008-02-28 Ning Huang Expression of human milk proteins in transgenic plants
EP1478379A4 (en) * 2001-12-28 2005-12-21 Campina Bv HUMANIZED LACTOFERRIN AND ITS APPLICATIONS
JP2006503545A (ja) * 2002-02-14 2006-02-02 ラター， ウィリアム ジェイ． 処置される宿主における切断についてのキメラ分子
WO2003099323A1 (en) 2002-05-10 2003-12-04 Agennix Incorporated Lactoferrin in the treatment of malignant neoplasms and other hyperproliferative diseases
US7097841B2 (en) * 2002-05-10 2006-08-29 New Century Pharmaceuticals, Inc. Ferritin fusion proteins for use in vaccines and other applications
AU2003233583A1 (en) * 2002-05-24 2003-12-12 Agennix Incorporated Oral lactoferrin in the treatment of respiratory disorders
ES2224792B1 (es) * 2002-06-28 2007-02-16 Era Plantech, S.L. Produccion de peptidos y proteinas por acumulacion de cuerpos proteicos derivados de reticulos endoplasmico en plantas.
JP4516844B2 (ja) * 2002-09-16 2010-08-04 エイジェニックス インコーポレイテッド 創傷の処置におけるラクトフェリン
US20040152624A1 (en) * 2002-12-06 2004-08-05 Atul Varadhachary Oral lactoferrin in the treatment of sepsis
AU2003296447A1 (en) 2002-12-10 2004-06-30 Agennix Incorporated Lactoferrin as an agent in the prevention of organ transplant rejection and graft-versus-host-disease
AU2003293500A1 (en) * 2002-12-12 2004-07-09 Agennix Incorporated Lactoferrin in the reduction of pain
WO2004103285A2 (en) * 2003-05-14 2004-12-02 Agennix Incorporated Lactoferrin in the treatment of diabetes mellitus
JP2007524654A (ja) * 2003-06-06 2007-08-30 エイジェニックス インコーポレイテッド 癌ワクチン中のアジュバントとしてのラクトフェリン
US20040259770A1 (en) * 2003-06-19 2004-12-23 Rush University Medical Center Method for determining leukocyte activation
WO2005018542A2 (en) * 2003-07-10 2005-03-03 Agennix Incorporated Use of lactoferrin in prophylaxis against infection and/or inflammation in immunosuppressed subjects
US7935501B2 (en) * 2003-09-11 2011-05-03 Novozymes Pharma Biotec A/S Recombinant production of antimicrobial peptides
WO2005035569A2 (en) * 2003-10-10 2005-04-21 Five Prime Therapeutics, Inc. Kiaa0779, splice variants thereof, and methods of their use
CA2548749A1 (en) * 2003-12-09 2005-06-23 Ventria Bioscience High-level expression of fusion polypeptides in plant seeds utilizing seed-storage proteins as fusion carriers
US7125963B2 (en) * 2004-03-03 2006-10-24 En N Tech Inc Treatments for contaminant reduction in lactoferrin preparations and lactoferrin containing compositions
WO2006047744A2 (en) * 2004-10-26 2006-05-04 Agennix Incorporated Compositions of lactoferrin related peptides and uses thereof
JP4634809B2 (ja) * 2005-01-05 2011-02-16 株式会社アップウェル アポタンパク質の製造方法
US20060211089A1 (en) * 2005-03-16 2006-09-21 Novozymes A/S Expression of defensins in filamentous fungi
CN101505781A (zh) 2006-06-22 2009-08-12 阿根尼克斯有限公司 作为辐射防护物质的乳铁蛋白
US9862956B2 (en) 2006-12-10 2018-01-09 Danisco Us Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
EP2102366A4 (en) 2006-12-10 2010-01-27 Dyadic International Inc EXPRESSION AND HIGH-DROP SCREENING OF COMPLEX EXPRESSED DNA LIBRARIES IN FADENED MUSHROOMS
NZ555163A (en) 2007-05-14 2010-05-28 Fonterra Co Operative Group Methods of immune or hematological enhancement, inhibiting tumour formation or growth, and treating or preventing cancer, cancer symptoms, or the symptoms of cancer treatments
WO2009019314A1 (en) 2007-08-08 2009-02-12 Novozymes A/S Transferrin variants and conjugates
EP2197893B1 (en) * 2007-09-07 2013-07-24 Dyadic International, Inc. Novel fungal enzymes
CN102348387B (zh) * 2009-01-28 2014-12-31 让-保罗·佩罗丹 生产乳转铁蛋白的方法
US20110294988A1 (en) 2009-02-06 2011-12-01 Novozymes Bio Pharma DK A/S Purification Process
EP3243835B1 (en) 2009-02-11 2024-04-10 Albumedix Ltd Albumin variants and conjugates
CA2758404A1 (en) * 2009-04-22 2010-10-28 Dsm Ip Assets B.V. Process for the production of a recombinant polypeptide of interest
WO2011104352A1 (en) 2010-02-25 2011-09-01 Agennix Ag Oral lactoferrin in the treatment of severe sepsis
EP2851421B1 (en) 2012-07-03 2018-11-28 Kao Corporation Useful microorganism, and method for producing substance of interest
CN103421794B (zh) * 2013-08-08 2014-07-02 华南理工大学 一种黑曲霉组成型表达元件、表达载体、重组黑曲霉及其构建方法和应用
CN108893415B (zh) * 2018-07-31 2022-12-27 上海凯圣生物科技有限公司 曲霉菌株及利用曲霉菌株发酵生产人乳铁蛋白的方法
CN119948159A (zh) * 2022-09-15 2025-05-06 诺维信公司 真菌信号肽
WO2025157268A1 (zh) * 2024-01-24 2025-07-31 南京百斯杰生物工程有限公司 一种米曲霉重组菌株的构建方法
WO2025226596A1 (en) * 2024-04-24 2025-10-30 Novozymes A/S Methods for producing secreted polypeptides
CN119776443B (zh) * 2024-12-23 2025-12-02 江苏创健医疗科技股份有限公司 一种多拷贝整合实现高效合成人乳铁蛋白的方法与应用
CN119841906B (zh) * 2025-01-26 2025-09-26 新苡萃(上海)生物科技有限公司 一种淀粉酶信号肽变体及其应用

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4766075A (en) * 1982-07-14 1988-08-23 Genentech, Inc. Human tissue plasminogen activator
US4652639A (en) * 1982-05-06 1987-03-24 Amgen Manufacture and expression of structural genes
US4703008A (en) * 1983-12-13 1987-10-27 Kiren-Amgen, Inc. DNA sequences encoding erythropoietin
US4710465A (en) * 1984-04-19 1987-12-01 Yale University Junction-fragment DNA probes and probe clusters
US4886747A (en) * 1985-03-22 1989-12-12 Genentech, Inc. Nucleic acid encoding TGF-β and its uses
US4959318A (en) * 1985-06-27 1990-09-25 Zymogenetics, Inc. Expression of protein C
US4965190A (en) * 1986-07-31 1990-10-23 Howard Hughes Medical Institute Methods for the identification of mutations in the human phenylalanine hydroxylase gene using DNA probes
US5019508A (en) * 1987-08-27 1991-05-28 Biotechnology Research Partners, Ltd. Synovial phospholipases
US5081227A (en) * 1988-03-04 1992-01-14 La Jolla Cancer Research Foundation Germ cell alkaline phosphatase
US5571691A (en) * 1989-05-05 1996-11-05 Baylor College Of Medicine Production of recombinant lactoferrin and lactoferrin polypeptides using CDNA sequences in various organisms
IL94183A (en) * 1989-05-05 2003-09-17 Baylor College Medicine cDNA SEQUENCE CODING FOR HUMAN LACTOFERRIN PROTEIN OR PORTION THEREOF AND LACTOFERRIN PROTEIN PRODUCED FROM SAID SEQUENCE
US5571697A (en) * 1989-05-05 1996-11-05 Baylor College Of Medicine Texas Medical Center Expression of processed recombinant lactoferrin and lactoferrin polypeptide fragments from a fusion product in Aspergillus
DK0429628T3 (da) * 1989-06-16 1999-05-31 Genencor Int DNA-sekvenser, vektorer og fusionspeptider til forøgelse af sekretion af ønskede polypeptider fra filamentøse fungi
UA27702C2 (uk) * 1989-09-27 2000-10-16 Гіст-Брокейдс Н.В. Фрагмент геномної днк, що кодує фітазу aspergillus niger,фрагмент кднк, що кодує фітазу aspergillus niger, рекомбінантна плазмідна днк для експресії фітази в aspergillus (варіанти), штам aspergillus-продуцент фітази aspergillus (варіанти), спосіб одержання фітази, рекомбінанта фітаза aspergillus niger
IL95697A0 (en) * 1989-09-28 1991-06-30 Granada Biosciences Inc Cdna sequence for porcine lactoferrin protein
AU7453391A (en) * 1990-03-08 1991-10-10 Ferrodynamics, Inc. Genetically engineered human lactoferrin
JP2818056B2 (ja) * 1990-09-07 1998-10-30 森永乳業株式会社 抗菌性ペプチドおよび抗菌剤
WO1992013550A1 (en) * 1991-02-08 1992-08-20 The University Of Vermont And State Agricultural College Recombinant transferrins, transferrin half-molecules and mutants thereof
DE69231995T2 (de) * 1991-12-09 2002-04-04 Unilever N.V., Rotterdam Verfahren zur Herstellung/Sekretion eines Proteins durch einen transformiertenSchimmelpilz unter Verwendung von Expressions-/Sekretions- Regulationsregionenaus Aspergillus
ZA932568B (en) * 1992-04-24 1993-11-12 Baylor College Midecine A Non Production of recombinant human lactoferrin

Also Published As

Publication number Publication date
KR100443307B1 (ko) 2004-11-08
AU4279896A (en) 1996-05-31
US6080559A (en) 2000-06-27
EP0871724A4 (OSRAM) 1998-10-21
WO1996014413A1 (en) 1996-05-17
DE871724T1 (de) 1999-06-02
KR970707283A (ko) 1997-12-01
CN1230537C (zh) 2005-12-07
US5571697A (en) 1996-11-05
ES2127164T1 (es) 1999-04-16
EP0871724A1 (en) 1998-10-21
JPH10509317A (ja) 1998-09-14
CN1171815A (zh) 1998-01-28
US5955316A (en) 1999-09-21
CA2204246A1 (en) 1996-05-17

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