MX388197B - Método para la reducción de proteínas de células hospederas en cromatografía de afinidad. - Google Patents

Método para la reducción de proteínas de células hospederas en cromatografía de afinidad.

Info

Publication number
MX388197B
MX388197B MX2018001536A MX2018001536A MX388197B MX 388197 B MX388197 B MX 388197B MX 2018001536 A MX2018001536 A MX 2018001536A MX 2018001536 A MX2018001536 A MX 2018001536A MX 388197 B MX388197 B MX 388197B
Authority
MX
Mexico
Prior art keywords
host cell
affinity chromatography
reduction
cell proteins
purifying
Prior art date
Application number
MX2018001536A
Other languages
English (en)
Spanish (es)
Other versions
MX2018001536A (es
Inventor
Annika Kleinjans
Benjamin Tran
Bernhard Spensberger
Carina Kopp
Frank Zettl
Klaus Schwendner
Marc Wong
Michael Wiedmann
Paul Mcdonald
John Richard St
Roberto Falkenstein
Ryan Erickson
Wolfgang Koehnlein
Original Assignee
Hoffmann La Roche
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=56802471&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX388197(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Hoffmann La Roche, Genentech Inc filed Critical Hoffmann La Roche
Publication of MX2018001536A publication Critical patent/MX2018001536A/es
Publication of MX388197B publication Critical patent/MX388197B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/10—RNA viruses
    • C07K16/108—Orthomyxoviridae (F), e.g. influenza virus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/244—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2809—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against the T-cell receptor (TcR)-CD3 complex
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2851—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the lectin superfamily, e.g. CD23, CD72
    • C07K16/2854—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the lectin superfamily, e.g. CD23, CD72 against selectins, e.g. CD62
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C07K16/3007—Carcino-embryonic Antigens
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/36—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against blood coagulation factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Oncology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
MX2018001536A 2015-08-21 2016-08-18 Método para la reducción de proteínas de células hospederas en cromatografía de afinidad. MX388197B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562208523P 2015-08-21 2015-08-21
PCT/EP2016/069604 WO2017032686A1 (en) 2015-08-21 2016-08-18 Method for the reduction of host cell proteins in affinity chromatography

Publications (2)

Publication Number Publication Date
MX2018001536A MX2018001536A (es) 2018-04-24
MX388197B true MX388197B (es) 2025-03-19

Family

ID=56802471

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2018001536A MX388197B (es) 2015-08-21 2016-08-18 Método para la reducción de proteínas de células hospederas en cromatografía de afinidad.

Country Status (14)

Country Link
US (3) US20180186832A1 (https=)
EP (1) EP3337812B1 (https=)
JP (4) JP6968055B2 (https=)
KR (2) KR102691585B1 (https=)
CN (2) CN107849087B (https=)
AU (1) AU2016312909B2 (https=)
CA (1) CA2992420A1 (https=)
ES (1) ES2877532T3 (https=)
HR (1) HRP20211026T1 (https=)
IL (1) IL257146B2 (https=)
MX (1) MX388197B (https=)
PL (1) PL3337812T3 (https=)
SI (1) SI3337812T1 (https=)
WO (1) WO2017032686A1 (https=)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2017003116A (es) 2014-09-10 2017-05-23 Procter & Gamble Trama de tela no tejida.
HRP20211737T1 (hr) 2015-08-21 2022-02-18 F. Hoffmann - La Roche Ag Postupak za smanjenje proteina stanice domaćina u afinitetnoj kromatografiji
EP3337818B1 (en) * 2015-08-21 2026-03-18 F. Hoffmann-La Roche AG Affinity chromatography purification with low conductivity wash buffer
SI3337812T1 (sl) 2015-08-21 2021-08-31 F. Hoffmann-La Roche Ag Postopek za zmanjševanje beljakovin gostiteljske celice v afinitetni kromatografiji
EP3788150A4 (en) * 2018-05-04 2022-01-26 Sigma-Aldrich Co. LLC PRODUCTION OF RECOMBINANT PROTEINS WITH REDUCED LEVELS OF HOST CELL PROTEINS
EP3826743A4 (en) * 2018-07-25 2022-09-14 Merck Sharp & Dohme Corp. METHODS FOR SEPARATION OF HOST CELL LIPASES FROM A PRODUCTION PROTEIN IN CHROMATOGRAPHIC METHODS
WO2020153390A1 (ja) * 2019-01-23 2020-07-30 第一三共株式会社 活性炭材料を使用する工程を含む抗体精製方法
JP2023500775A (ja) * 2019-09-23 2023-01-11 メルク・シャープ・アンド・ドーム・エルエルシー 宿主細胞タンパク質が減少し、ポリソルベート－８０安定性が増加した、抗ｃｔｌａ４モノクローナル抗体を含む方法および組成物
WO2023284073A1 (zh) * 2021-07-13 2023-01-19 江苏荃信生物医药股份有限公司 降低单克隆抗体生产中宿主细胞蛋白含量的亲和纯化方法、抗人ifnar1单克隆抗体浓缩溶液的制备方法以及液体制剂
US20250223313A1 (en) * 2022-01-14 2025-07-10 Shattuck Labs, Inc. Methods of contaminant removal from protein isolates
WO2024178239A1 (en) * 2023-02-22 2024-08-29 Shattuck Labs, Inc. Engineered cell lines and uses thereof

Family Cites Families (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1400536A1 (en) 1991-06-14 2004-03-24 Genentech Inc. Method for making humanized antibodies
GB9203459D0 (en) 1992-02-19 1992-04-08 Scotgen Ltd Antibodies with germ-line variable regions
DE69738075T2 (de) 1996-11-27 2008-05-21 Genentech, Inc., South San Francisco Affinitätsreinigung von polypeptiden an einer protein a-matrix
DE1257584T1 (de) 2000-02-24 2003-05-28 Lilly Co Eli Humanisierte antikörper, die amyloid beta peptid demarkieren
WO2002072615A1 (en) 2001-03-09 2002-09-19 Chugai Seiyaku Kabushiki Kaisha Method of purifying protein
TWI338009B (en) 2001-10-29 2011-03-01 Genentech Inc Antibodies for inhibiting blood coagulation and methods of use thereof
AR038568A1 (es) 2002-02-20 2005-01-19 Hoffmann La Roche Anticuerpos anti-a beta y su uso
EP3225625A1 (en) 2002-09-11 2017-10-04 Chugai Seiyaku Kabushiki Kaisha Protein purification method
ATE468886T1 (de) 2003-02-10 2010-06-15 Applied Molecular Evolution Abeta-bindende moleküle
PT1737891E (pt) 2004-04-13 2013-04-16 Hoffmann La Roche Anticorpos anti p-selectina
CA2581208A1 (en) 2004-08-30 2006-03-09 Lonza Biologics Plc. Affinity- plus ion exchange- chromatography for purifying antibodies
TWI306862B (en) 2005-01-03 2009-03-01 Hoffmann La Roche Antibodies against il-13 receptor alpha 1 and uses thereof
PT1876236E (pt) 2005-04-08 2014-10-22 Chugai Pharmaceutical Co Ltd Anticorpos para substituição da função do factor de coagulação sanguínea viii
ZA200710330B (en) 2005-06-17 2009-12-30 Wyeth Corp Methods of purifying FC region containing proteins
MY155286A (en) * 2005-12-12 2015-09-30 Hoffmann La Roche Antibodies against amyloid beta 4 with glycosylated in the variable region
WO2007109163A2 (en) 2006-03-16 2007-09-27 Amgen Inc Wash buffer and method of using
EP2046833B9 (en) 2006-07-14 2014-02-19 AC Immune S.A. Humanized antibody against amyloid beta
RU2474585C2 (ru) 2007-01-22 2013-02-10 Дженентек, Инк. Осаждение и очистка белков полиэлектролитами
EP2278999B1 (en) * 2008-04-21 2025-01-29 Dompé farmaceutici S.p.A. Auris formulations for treating otic diseases and conditions
RU2474588C2 (ru) 2008-05-05 2013-02-10 Новиммун Са Перекрестно-реактивные антитела анти-il-17a/il-17f и способы их применения
EP2116556B1 (en) 2008-05-09 2016-03-23 AbbVie Deutschland GmbH & Co KG Antibodies to receptor of advanced glycation end products (RAGE) and uses thereof
BRPI0919879A2 (pt) 2008-10-29 2016-02-16 Wyeth Llc métodos para purificação de moléculas de ligação a antígeno de domínio único
WO2011038894A1 (en) * 2009-10-01 2011-04-07 F. Hoffmann-La Roche Ag Protein a chromatography
ES2982297T3 (es) 2009-12-18 2024-10-15 Novartis Ag Método de cromatografía de afinidad
AR080794A1 (es) 2010-03-26 2012-05-09 Hoffmann La Roche Anticuerpos bivalentes biespecificos anti- vegf/ anti-ang-2
KR101860963B1 (ko) 2010-04-23 2018-05-24 제넨테크, 인크. 이종다량체 단백질의 생산
EP2583973B1 (en) 2010-06-21 2018-03-21 Kyowa Hakko Kirin Co., Ltd. Method for purifying protein using amino acid
WO2012047732A2 (en) 2010-09-29 2012-04-12 Genentech, Inc. Antibody compositions and methods of use
TWI452136B (zh) 2010-11-17 2014-09-11 中外製藥股份有限公司 A multiple specific antigen-binding molecule that replaces the function of Factor VIII in blood coagulation
EP2714714B1 (en) 2011-06-01 2018-06-20 Novartis AG Wash solution and method for affinity chromatography
WO2013033517A1 (en) 2011-09-01 2013-03-07 Human Genome Sciences, Inc. Viral clearance methods
HUE035685T2 (en) 2011-11-02 2018-05-28 Hoffmann La Roche Overload and elute chromatography
EP2849723B1 (en) * 2012-05-18 2018-05-02 Genentech, Inc. High-concentration monoclonal antibody formulations
TW201348247A (zh) 2012-05-21 2013-12-01 Abbvie Inc 利用蛋白質ａ親和性層析之非人類抗體之新穎純化
UA118028C2 (uk) 2013-04-03 2018-11-12 Рош Глікарт Аг Біспецифічне антитіло, специфічне щодо fap і dr5, антитіло, специфічне щодо dr5, і спосіб їх застосування
EP2997036B1 (en) 2013-05-15 2021-03-03 Medimmune Limited Purification of recombinantly produced polypeptides
TWI596107B (zh) * 2013-06-25 2017-08-21 卡地拉保健有限公司 單株抗體之新穎純化方法
US10144774B2 (en) * 2013-07-01 2018-12-04 Csl Behring Ag Method for purifying IgG
CN105473612A (zh) * 2013-08-19 2016-04-06 豪夫迈·罗氏有限公司 用羟基磷灰石层析分离双特异性抗体和双特异性抗体生产副产物
CN120904322A (zh) * 2013-09-13 2025-11-07 豪夫迈·罗氏有限公司 包含纯化的重组多肽的方法和组合物
JPWO2015041218A1 (ja) 2013-09-17 2017-03-02 株式会社カネカ 新規抗体精製方法及びそれから得られる抗体（ＮｏｖｅｌＡｎｔｉｂｏｄｙＰｕｒｉｆｉｃａｔｉｏｎＭｅｔｈｏｄａｎｄＡｎｔｉｂｏｄｙｏｂｔａｉｎｅｄｔｈｅｒｅｆｒｏｍ）、並びに陽イオン交換基を用いた新規抗体精製法及びそれから得られる抗体（ＮｏｖｅｌＡｎｔｉｂｏｄｙＰｕｒｉｆｉｃａｔｉｏｎｍｅｔｈｏｄｕｓｉｎｇＣａｔｉｏｎＥｘｃｈａｎｇｅｒａｎｄＡｎｔｉｂｏｄｙｏｂｔａｉｎｅｄｔｈｅｒｅｆｒｏｍ）
JP2015083558A (ja) 2013-09-18 2015-04-30 東ソー株式会社 抗体吸着剤ならびにそれを用いた抗体の精製方法および識別方法
CN103497248B (zh) * 2013-09-22 2016-08-10 中国抗体制药有限公司 一种从细胞培养上清中分离纯化抗体的方法
US20160286398A1 (en) 2015-03-23 2016-09-29 Qualcomm Incorporated Schedule selection and connection setup between devices participating in a nan data link
HRP20211737T1 (hr) 2015-08-21 2022-02-18 F. Hoffmann - La Roche Ag Postupak za smanjenje proteina stanice domaćina u afinitetnoj kromatografiji
EP3337818B1 (en) 2015-08-21 2026-03-18 F. Hoffmann-La Roche AG Affinity chromatography purification with low conductivity wash buffer
SI3337812T1 (sl) 2015-08-21 2021-08-31 F. Hoffmann-La Roche Ag Postopek za zmanjševanje beljakovin gostiteljske celice v afinitetni kromatografiji
JP2021124951A (ja) 2020-02-05 2021-08-30 富士通株式会社 情報処理システム、情報処理装置およびアクセス制御方法

Also Published As

Publication number Publication date
JP6968055B2 (ja) 2021-11-17
ES2877532T3 (es) 2021-11-17
IL257146A (en) 2018-03-29
EP3337812A1 (en) 2018-06-27
JP7610638B2 (ja) 2025-01-08
KR20240121902A (ko) 2024-08-09
CN116063375A (zh) 2023-05-05
AU2016312909A1 (en) 2018-02-22
AU2016312909B2 (en) 2022-12-08
CA2992420A1 (en) 2017-03-02
MX2018001536A (es) 2018-04-24
WO2017032686A1 (en) 2017-03-02
JP2021176900A (ja) 2021-11-11
EP3337812B1 (en) 2021-04-28
JP7258964B2 (ja) 2023-04-17
JP2023076617A (ja) 2023-06-01
IL257146B1 (en) 2024-09-01
US20260085090A1 (en) 2026-03-26
CN107849087A (zh) 2018-03-27
CN107849087B (zh) 2022-09-06
US20220169675A1 (en) 2022-06-02
KR20180034500A (ko) 2018-04-04
KR102691585B1 (ko) 2024-08-05
JP2025031829A (ja) 2025-03-07
HRP20211026T1 (hr) 2021-10-01
HK1251584A1 (zh) 2019-02-01
IL257146B2 (en) 2025-01-01
US20180186832A1 (en) 2018-07-05
SI3337812T1 (sl) 2021-08-31
BR112018001511A2 (pt) 2018-09-18
JP2018531895A (ja) 2018-11-01
US12503487B2 (en) 2025-12-23
PL3337812T3 (pl) 2021-10-11

Similar Documents

Publication Publication Date Title
MX388197B (es) Método para la reducción de proteínas de células hospederas en cromatografía de afinidad.
MX377315B (es) Predicción in vitro de semivida in vivo de anticuerpos.
BR112014018995A2 (pt) Sistemas para executar ensaio automatizado
PE20210107A1 (es) Anticuerpos anti-cd3 y metodos de uso
MA41313A (fr) Anticorps de liaison de la protéine april modifiés
EP3543335A3 (en) Soluble intein fusion proteins and methods for purifying biomolecules
MA38500A1 (fr) Systèmes et procédés pour analyser une nourriture pour animal
MX2017000083A (es) Compuestos de platino, composiciones y usos de estos.
CU20160008A7 (es) Métodos para controlar los niveles de fucosilacion en proteinas
EA201501028A1 (ru) Конструкции митохондриальных белков и их применение
JOP20210010A1 (ar) بروتينات مخصصة ل baff و b7rp1 وإستخداماتها
BR112016013371A2 (pt) melhorias em proteína de fusão pesticida
TR201818966T4 (tr) İyon değişim kromatografisi girdi optimizasyonunun açıklaması.
MX380183B (es) Control de la formacion de enlaces disulfuro en disoluciones de proteinas mediante la adicion de agentes reductores.
EA201790725A1 (ru) Основанные на близости расположения способы выбора партнеров для связывания
SI3337817T1 (sl) Postopek za redukcijo beljakovin gostiteljske celice pri afinitetni kromatografiji
MX384636B (es) Método para detectar anticuerpos neutralizantes contra la insulina humana recombinante en suero humano.
MX390567B (es) Metodos y sistemas para extraer proteina de acido oxalico reducido de especies acuaticas y composiciones de la misma
MX384990B (es) Selectividad eficaz de proteínas recombinantes.
IL265195A (en) Protein biomarkers for diseases associated with the contact activation system
MX2015010006A (es) Purificacion de proteinas mejorada a traves de una etapa de desglicosilacion.
MY185585A (en) Method for determining dimethyl disulphide
MY170201A (en) A method for identifying a ganoderma-infected palm tree
MY168633A (en) Biomarker for severe dengue
TH12414C3 (th) กรรมวิธีการวัดปริมาณโปรตีนไกลเคทเตดอัลบูมินโดยการใช้สารละลายกราฟีนร่วมกับแอปตาเมอร์