MX2018001536A - Metodo para la reduccion de proteinas de celulas hospederas en cromatografia de afinidad. - Google Patents

Metodo para la reduccion de proteinas de celulas hospederas en cromatografia de afinidad.

Info

Publication number
MX2018001536A
MX2018001536A MX2018001536A MX2018001536A MX2018001536A MX 2018001536 A MX2018001536 A MX 2018001536A MX 2018001536 A MX2018001536 A MX 2018001536A MX 2018001536 A MX2018001536 A MX 2018001536A MX 2018001536 A MX2018001536 A MX 2018001536A
Authority
MX
Mexico
Prior art keywords
host cell
affinity chromatography
reduction
cell proteins
purifying
Prior art date
Application number
MX2018001536A
Other languages
English (en)
Inventor
Falkenstein Roberto
Schwendner Klaus
Spensberger Bernhard
Mcdonald Paul
St John Richard
Wong Marc
Koehnlein Wolfgang
Wiedmann Michael
Zettl Frank
Kleinjans Annika
KOPP Carina
Erickson Ryan
Tran Benjamin
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=56802471&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2018001536(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of MX2018001536A publication Critical patent/MX2018001536A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
    • C07K16/1018—Orthomyxoviridae, e.g. influenza virus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/244—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2809—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against the T-cell receptor (TcR)-CD3 complex
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2851—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the lectin superfamily, e.g. CD23, CD72
    • C07K16/2854—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the lectin superfamily, e.g. CD23, CD72 against selectins, e.g. CD62
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C07K16/3007—Carcino-embryonic Antigens
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/36—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against blood coagulation factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Oncology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Communicable Diseases (AREA)
  • Pulmonology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

La presente invención se relaciona con un método para purificar un anticuerpo reduciendo el contenido de una proteína de la célula hospedera. El método emplea una etapa de lavado con una solución acuosa de baja conductividad en una cromatografía de afinidad.
MX2018001536A 2015-08-21 2016-08-18 Metodo para la reduccion de proteinas de celulas hospederas en cromatografia de afinidad. MX2018001536A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562208523P 2015-08-21 2015-08-21
PCT/EP2016/069604 WO2017032686A1 (en) 2015-08-21 2016-08-18 Method for the reduction of host cell proteins in affinity chromatography

Publications (1)

Publication Number Publication Date
MX2018001536A true MX2018001536A (es) 2018-04-24

Family

ID=56802471

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2018001536A MX2018001536A (es) 2015-08-21 2016-08-18 Metodo para la reduccion de proteinas de celulas hospederas en cromatografia de afinidad.

Country Status (16)

Country Link
US (2) US20180186832A1 (es)
EP (1) EP3337812B1 (es)
JP (3) JP6968055B2 (es)
KR (1) KR20180034500A (es)
CN (2) CN116063375A (es)
AU (1) AU2016312909B2 (es)
BR (1) BR112018001511A2 (es)
CA (1) CA2992420A1 (es)
ES (1) ES2877532T3 (es)
HK (1) HK1251584A1 (es)
HR (1) HRP20211026T1 (es)
IL (1) IL257146A (es)
MX (1) MX2018001536A (es)
PL (1) PL3337812T3 (es)
SI (1) SI3337812T1 (es)
WO (1) WO2017032686A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3193801B1 (en) 2014-09-10 2022-07-13 The Procter & Gamble Company Nonwoven web
CN107835819A (zh) 2015-08-21 2018-03-23 豪夫迈·罗氏有限公司 在亲和层析中减少宿主细胞蛋白的方法
ES2877532T3 (es) 2015-08-21 2021-11-17 Hoffmann La Roche Procedimiento para la reducción de proteínas de célula huésped en cromatografía de afinidad
SG11202009499VA (en) * 2018-05-04 2020-11-27 Sigma Aldrich Co Llc Producing recombinant proteins with reduced levels of host cell proteins
EP3826743A4 (en) * 2018-07-25 2022-09-14 Merck Sharp & Dohme Corp. METHODS FOR SEPARATION OF HOST CELL LIPASES FROM A PRODUCTION PROTEIN IN CHROMATOGRAPHIC METHODS
AU2020356303A1 (en) * 2019-09-23 2022-04-14 Merck Sharp & Dohme Llc Methods and compositions comprising an anti-CTLA4 monoclonal antibody with reduced host cell proteins and increased polysorbate-80 stability
WO2023284073A1 (zh) * 2021-07-13 2023-01-19 江苏荃信生物医药股份有限公司 降低单克隆抗体生产中宿主细胞蛋白含量的亲和纯化方法、抗人ifnar1单克隆抗体浓缩溶液的制备方法以及液体制剂
WO2023137143A1 (en) * 2022-01-14 2023-07-20 Shattuck Labs, Inc. Methods of contaminant removal from protein isolates

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2261230T3 (en) 2002-09-11 2017-06-12 Chugai Pharmaceutical Co Ltd Method of Protein Purification.
TW200902555A (en) 2005-01-03 2009-01-16 Hoffmann La Roche Antibodies against IL-13 receptor alpha 1 and uses thereof
DOP2006000278A (es) * 2005-12-12 2007-07-15 Hoffmann La Roche Glicosilación en la región variable
US8263750B2 (en) 2006-03-16 2012-09-11 Amgen Inc. Method for purifying a protein using protein-A affinity chromatography using an intermediate wash step
WO2011038894A1 (en) * 2009-10-01 2011-04-07 F. Hoffmann-La Roche Ag Protein a chromatography
SG10201408384PA (en) 2009-12-18 2015-01-29 Novartis Ag Wash solution and method for affinity chromatography
WO2011162210A1 (ja) * 2010-06-21 2011-12-29 協和発酵キリン株式会社 アミノ酸を利用したタンパク質の精製方法
US10259842B2 (en) 2013-05-15 2019-04-16 Medimmune, Llc Purification of recombinantly produced polypeptides
AR096713A1 (es) * 2013-06-25 2016-01-27 Cadila Healthcare Ltd Proceso de purificación para anticuerpos monoclonales
EP3016976B1 (en) * 2013-07-01 2020-09-09 CSL Behring AG Process
BR112016003196A8 (pt) * 2013-08-19 2020-02-04 Hoffmann La Roche método para separar anticorpos biespecíficos
NZ756749A (en) * 2013-09-13 2022-05-27 Genentech Inc Methods and compositions comprising purified recombinant polypeptides
CN103497248B (zh) * 2013-09-22 2016-08-10 中国抗体制药有限公司 一种从细胞培养上清中分离纯化抗体的方法
CN107835819A (zh) 2015-08-21 2018-03-23 豪夫迈·罗氏有限公司 在亲和层析中减少宿主细胞蛋白的方法
ES2877532T3 (es) 2015-08-21 2021-11-17 Hoffmann La Roche Procedimiento para la reducción de proteínas de célula huésped en cromatografía de afinidad

Also Published As

Publication number Publication date
HK1251584A1 (zh) 2019-02-01
KR20180034500A (ko) 2018-04-04
JP7258964B2 (ja) 2023-04-17
BR112018001511A2 (pt) 2018-09-18
PL3337812T3 (pl) 2021-10-11
HRP20211026T1 (hr) 2021-10-01
AU2016312909A1 (en) 2018-02-22
CA2992420A1 (en) 2017-03-02
ES2877532T3 (es) 2021-11-17
JP2018531895A (ja) 2018-11-01
JP6968055B2 (ja) 2021-11-17
US20220169675A1 (en) 2022-06-02
US20180186832A1 (en) 2018-07-05
IL257146A (en) 2018-03-29
CN107849087A (zh) 2018-03-27
CN107849087B (zh) 2022-09-06
EP3337812B1 (en) 2021-04-28
AU2016312909B2 (en) 2022-12-08
CN116063375A (zh) 2023-05-05
JP2023076617A (ja) 2023-06-01
EP3337812A1 (en) 2018-06-27
WO2017032686A1 (en) 2017-03-02
SI3337812T1 (sl) 2021-08-31
JP2021176900A (ja) 2021-11-11

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