HK1249122A1 - 用低電導率洗滌緩衝液進行親和層析純化 - Google Patents

用低電導率洗滌緩衝液進行親和層析純化

Info

Publication number
HK1249122A1
HK1249122A1 HK18108786.1A HK18108786A HK1249122A1 HK 1249122 A1 HK1249122 A1 HK 1249122A1 HK 18108786 A HK18108786 A HK 18108786A HK 1249122 A1 HK1249122 A1 HK 1249122A1
Authority
HK
Hong Kong
Prior art keywords
affinity chromatography
wash buffer
chromatography purification
low conductivity
conductivity wash
Prior art date
Application number
HK18108786.1A
Other languages
English (en)
Inventor
Roberto Falkenstein
Eva Rosenberg
Agathe Bialas
Steffen Willmann
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of HK1249122A1 publication Critical patent/HK1249122A1/zh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/36—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against blood coagulation factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C07K2317/522—CH1 domain
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Oncology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
HK18108786.1A 2015-08-21 2018-07-06 用低電導率洗滌緩衝液進行親和層析純化 HK1249122A1 (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP15181902 2015-08-21
PCT/EP2016/069162 WO2017032610A1 (en) 2015-08-21 2016-08-11 Affinity chromatography purification with low conductivity wash buffer

Publications (1)

Publication Number Publication Date
HK1249122A1 true HK1249122A1 (zh) 2018-10-26

Family

ID=54064132

Family Applications (1)

Application Number Title Priority Date Filing Date
HK18108786.1A HK1249122A1 (zh) 2015-08-21 2018-07-06 用低電導率洗滌緩衝液進行親和層析純化

Country Status (6)

Country Link
US (2) US20180186866A1 (zh)
EP (1) EP3337818A1 (zh)
JP (1) JP7084301B2 (zh)
CN (1) CN107849122B (zh)
HK (1) HK1249122A1 (zh)
WO (1) WO2017032610A1 (zh)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9840553B2 (en) 2014-06-28 2017-12-12 Kodiak Sciences Inc. Dual PDGF/VEGF antagonists
IL260323B1 (en) 2015-12-30 2024-09-01 Kodiak Sciences Inc Antibodies and their conjugates
US12071476B2 (en) 2018-03-02 2024-08-27 Kodiak Sciences Inc. IL-6 antibodies and fusion constructs and conjugates thereof
CN109336969B (zh) * 2018-11-09 2021-02-12 杭州奕安济世生物药业有限公司 一种抗体的纯化方法
US11912784B2 (en) 2019-10-10 2024-02-27 Kodiak Sciences Inc. Methods of treating an eye disorder
CA3162365A1 (en) * 2019-12-20 2021-06-24 Merck Sharp & Dohme Llc Purified compositions of enteroviruses and methods of purification with glutathione affinity chromatography
TWI787710B (zh) * 2020-01-20 2022-12-21 中國大陸商上海藥明生物技術有限公司 用於親和層析的新型清洗緩衝液
WO2022222949A1 (zh) * 2021-04-23 2022-10-27 和铂医药(上海)有限责任公司 一种双特异性抗体的纯化方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9203459D0 (en) * 1992-02-19 1992-04-08 Scotgen Ltd Antibodies with germ-line variable regions
EP3088412B1 (en) * 2001-03-09 2021-05-05 Chugai Seiyaku Kabushiki Kaisha Protein purification method
CN100522989C (zh) * 2002-09-11 2009-08-05 中外制药株式会社 纯化蛋白质的方法
WO2006024497A1 (en) * 2004-08-30 2006-03-09 Lonza Biologics Plc. Affinity- plus ion exchange- chromatography for purifying antibodies
AR053633A1 (es) * 2005-06-17 2007-05-09 Wyeth Corp Metodos para purificar proteinas que contienen una region fc
JP6113404B2 (ja) * 2008-10-29 2017-04-12 アブリンクス エン．ヴェー． 単一ドメイン抗原結合分子の精製方法
AR080794A1 (es) * 2010-03-26 2012-05-09 Hoffmann La Roche Anticuerpos bivalentes biespecificos anti- vegf/ anti-ang-2
EP3428176A1 (en) * 2011-06-01 2019-01-16 Novartis AG Wash solution and method for affinity chromatography
BR112014010406A2 (pt) * 2011-11-02 2017-04-25 Genentech Inc cromatografia de sobrecarga e eluto
UA118028C2 (uk) * 2013-04-03 2018-11-12 Рош Глікарт Аг Біспецифічне антитіло, специфічне щодо fap і dr5, антитіло, специфічне щодо dr5, і спосіб їх застосування
KR20160044023A (ko) * 2013-08-19 2016-04-22 에프. 호프만-라 로슈 아게 히드록시아파타이트 크로마토그래피를 사용하는 이중특이적 항체 및 이중특이적 항체 생산 부산물의 분리
EP3048109A4 (en) * 2013-09-17 2017-04-19 Kaneka Corporation Novel antibody purification method and antibody obtained therefrom, and novel antibody purification method using cation exchanger and antibody obtained therefrom
CA2992420A1 (en) * 2015-08-21 2017-03-02 F. Hoffmann-La Roche Ag Method for the reduction of host cell proteins in affinity chromatography

Also Published As

Publication number Publication date
WO2017032610A1 (en) 2017-03-02
US20220119500A1 (en) 2022-04-21
JP7084301B2 (ja) 2022-06-14
CN107849122A (zh) 2018-03-27
EP3337818A1 (en) 2018-06-27
US20180186866A1 (en) 2018-07-05
CN107849122B (zh) 2022-01-25
JP2018525407A (ja) 2018-09-06

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