KR102691585B1 - 친화성 크로마토그래피에서의 숙주 세포 단백질 감소 방법 - Google Patents

친화성 크로마토그래피에서의 숙주 세포 단백질 감소 방법 Download PDF

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KR102691585B1
KR102691585B1 KR1020187004868A KR20187004868A KR102691585B1 KR 102691585 B1 KR102691585 B1 KR 102691585B1 KR 1020187004868 A KR1020187004868 A KR 1020187004868A KR 20187004868 A KR20187004868 A KR 20187004868A KR 102691585 B1 KR102691585 B1 KR 102691585B1
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antibody
aqueous solution
tris
conductivity aqueous
protein
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KR20180034500A (ko
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폴 맥도날드
존 리차드 세인트
마크 웡
로베르토 팔켄슈타인
볼프강 쾬라인
볼프강 ??라인
클라우스 슈벤드너
베른하르트 슈펜스베르거
미하엘 비드만
프랑크 체틀
아니카 클라인얀스
카리나 코프
벤자민 트렌
라이언 에릭슨
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에프. 호프만-라 로슈 아게
제넨테크, 인크.
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Application filed by 에프. 호프만-라 로슈 아게, 제넨테크, 인크. filed Critical 에프. 호프만-라 로슈 아게
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/10—RNA viruses
    • C07K16/108—Orthomyxoviridae (F), e.g. influenza virus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/244—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2809—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against the T-cell receptor (TcR)-CD3 complex
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2851—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the lectin superfamily, e.g. CD23, CD72
    • C07K16/2854—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the lectin superfamily, e.g. CD23, CD72 against selectins, e.g. CD62
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C07K16/3007—Carcino-embryonic Antigens
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/36—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against blood coagulation factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Oncology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
KR1020187004868A 2015-08-21 2016-08-18 친화성 크로마토그래피에서의 숙주 세포 단백질 감소 방법 Active KR102691585B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020247025729A KR20240121902A (ko) 2015-08-21 2016-08-18 친화성 크로마토그래피에서의 숙주 세포 단백질 감소 방법

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562208523P 2015-08-21 2015-08-21
US62/208,523 2015-08-21
PCT/EP2016/069604 WO2017032686A1 (en) 2015-08-21 2016-08-18 Method for the reduction of host cell proteins in affinity chromatography

Related Child Applications (1)

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KR1020247025729A Division KR20240121902A (ko) 2015-08-21 2016-08-18 친화성 크로마토그래피에서의 숙주 세포 단백질 감소 방법

Publications (2)

Publication Number Publication Date
KR20180034500A KR20180034500A (ko) 2018-04-04
KR102691585B1 true KR102691585B1 (ko) 2024-08-05

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KR1020187004868A Active KR102691585B1 (ko) 2015-08-21 2016-08-18 친화성 크로마토그래피에서의 숙주 세포 단백질 감소 방법
KR1020247025729A Pending KR20240121902A (ko) 2015-08-21 2016-08-18 친화성 크로마토그래피에서의 숙주 세포 단백질 감소 방법

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Country Status (14)

Country Link
US (3) US20180186832A1 (https=)
EP (1) EP3337812B1 (https=)
JP (4) JP6968055B2 (https=)
KR (2) KR102691585B1 (https=)
CN (2) CN107849087B (https=)
AU (1) AU2016312909B2 (https=)
CA (1) CA2992420A1 (https=)
ES (1) ES2877532T3 (https=)
HR (1) HRP20211026T1 (https=)
IL (1) IL257146B2 (https=)
MX (1) MX388197B (https=)
PL (1) PL3337812T3 (https=)
SI (1) SI3337812T1 (https=)
WO (1) WO2017032686A1 (https=)

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EP3337818B1 (en) * 2015-08-21 2026-03-18 F. Hoffmann-La Roche AG Affinity chromatography purification with low conductivity wash buffer
SI3337812T1 (sl) 2015-08-21 2021-08-31 F. Hoffmann-La Roche Ag Postopek za zmanjševanje beljakovin gostiteljske celice v afinitetni kromatografiji
EP3788150A4 (en) * 2018-05-04 2022-01-26 Sigma-Aldrich Co. LLC PRODUCTION OF RECOMBINANT PROTEINS WITH REDUCED LEVELS OF HOST CELL PROTEINS
EP3826743A4 (en) * 2018-07-25 2022-09-14 Merck Sharp & Dohme Corp. METHODS FOR SEPARATION OF HOST CELL LIPASES FROM A PRODUCTION PROTEIN IN CHROMATOGRAPHIC METHODS
WO2020153390A1 (ja) * 2019-01-23 2020-07-30 第一三共株式会社 活性炭材料を使用する工程を含む抗体精製方法
JP2023500775A (ja) * 2019-09-23 2023-01-11 メルク・シャープ・アンド・ドーム・エルエルシー 宿主細胞タンパク質が減少し、ポリソルベート－８０安定性が増加した、抗ｃｔｌａ４モノクローナル抗体を含む方法および組成物
WO2023284073A1 (zh) * 2021-07-13 2023-01-19 江苏荃信生物医药股份有限公司 降低单克隆抗体生产中宿主细胞蛋白含量的亲和纯化方法、抗人ifnar1单克隆抗体浓缩溶液的制备方法以及液体制剂
US20250223313A1 (en) * 2022-01-14 2025-07-10 Shattuck Labs, Inc. Methods of contaminant removal from protein isolates
WO2024178239A1 (en) * 2023-02-22 2024-08-29 Shattuck Labs, Inc. Engineered cell lines and uses thereof

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Publication number Publication date
MX388197B (es) 2025-03-19
JP6968055B2 (ja) 2021-11-17
ES2877532T3 (es) 2021-11-17
IL257146A (en) 2018-03-29
EP3337812A1 (en) 2018-06-27
JP7610638B2 (ja) 2025-01-08
KR20240121902A (ko) 2024-08-09
CN116063375A (zh) 2023-05-05
AU2016312909A1 (en) 2018-02-22
AU2016312909B2 (en) 2022-12-08
CA2992420A1 (en) 2017-03-02
MX2018001536A (es) 2018-04-24
WO2017032686A1 (en) 2017-03-02
JP2021176900A (ja) 2021-11-11
EP3337812B1 (en) 2021-04-28
JP7258964B2 (ja) 2023-04-17
JP2023076617A (ja) 2023-06-01
IL257146B1 (en) 2024-09-01
US20260085090A1 (en) 2026-03-26
CN107849087A (zh) 2018-03-27
CN107849087B (zh) 2022-09-06
US20220169675A1 (en) 2022-06-02
KR20180034500A (ko) 2018-04-04
JP2025031829A (ja) 2025-03-07
HRP20211026T1 (hr) 2021-10-01
HK1251584A1 (zh) 2019-02-01
IL257146B2 (en) 2025-01-01
US20180186832A1 (en) 2018-07-05
SI3337812T1 (sl) 2021-08-31
BR112018001511A2 (pt) 2018-09-18
JP2018531895A (ja) 2018-11-01
US12503487B2 (en) 2025-12-23
PL3337812T3 (pl) 2021-10-11

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