MX374236B - Prueba mejorada de endotoxina bacteriana para determinación de endotoxinas. - Google Patents

Prueba mejorada de endotoxina bacteriana para determinación de endotoxinas.

Info

Publication number
MX374236B
MX374236B MX2018000679A MX2018000679A MX374236B MX 374236 B MX374236 B MX 374236B MX 2018000679 A MX2018000679 A MX 2018000679A MX 2018000679 A MX2018000679 A MX 2018000679A MX 374236 B MX374236 B MX 374236B
Authority
MX
Mexico
Prior art keywords
sample
bacterial endotoxin
endotoxins
determination
endotoxin test
Prior art date
Application number
MX2018000679A
Other languages
English (en)
Other versions
MX2018000679A (es
Inventor
Christian Alexander
Wintzingerode Friedrich Von
Pierre Lang
Sven Deutschmann
Ulrich Zaehringer
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of MX2018000679A publication Critical patent/MX2018000679A/es
Publication of MX374236B publication Critical patent/MX374236B/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/579—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving limulus lysate
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
    • G01N2400/10—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • G01N2400/50—Lipopolysaccharides; LPS
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicinal Preparation (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invención se refiere a un medio para determinar endotoxina bacteriana a bajas concentraciones en una muestra de un anticuerpo (que se ha producido usando células bacterianas) que comprende los siguientes pasos en el siguiente orden: (i) adicionar iones magnesio a la muestra (ii) diluir la muestra, (iii) dializar la muestra que tiene un valor de pH de 5.7-8.0 contra una solución acuosa libre de endotoxina, y (iv) determinar la endotoxina bacteriana en la muestra usando una prueba de endotoxina bacteriana, particularmente el ensayo de lisado de amebocitos de Limulus.
MX2018000679A 2015-07-28 2016-07-27 Prueba mejorada de endotoxina bacteriana para determinación de endotoxinas. MX374236B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP15178683.7A EP3124976B1 (en) 2015-07-28 2015-07-28 Improved bacterial endotoxin test for the determination of endotoxins
PCT/EP2016/067896 WO2017017135A1 (en) 2015-07-28 2016-07-27 Improved bacterial endotoxin test for the determination of endotoxins

Publications (2)

Publication Number Publication Date
MX2018000679A MX2018000679A (es) 2018-11-29
MX374236B true MX374236B (es) 2025-03-05

Family

ID=53776384

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2018000679A MX374236B (es) 2015-07-28 2016-07-27 Prueba mejorada de endotoxina bacteriana para determinación de endotoxinas.

Country Status (21)

Country Link
US (2) US10585097B2 (es)
EP (1) EP3124976B1 (es)
JP (2) JP6800226B2 (es)
KR (1) KR102630300B1 (es)
CN (2) CN108027374B (es)
AR (1) AR105497A1 (es)
AU (1) AU2016298598B2 (es)
BR (1) BR112018001748A8 (es)
CA (1) CA2989960C (es)
DK (1) DK3124976T3 (es)
ES (1) ES2699526T3 (es)
HR (1) HRP20181954T1 (es)
IL (2) IL256660B (es)
LT (1) LT3124976T (es)
MX (1) MX374236B (es)
PL (1) PL3124976T3 (es)
PT (1) PT3124976T (es)
RS (1) RS58167B1 (es)
SI (1) SI3124976T1 (es)
TR (1) TR201816369T4 (es)
WO (1) WO2017017135A1 (es)

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Publication number Priority date Publication date Assignee Title
US11099189B2 (en) 2017-10-17 2021-08-24 Board Of Regents, The University Of Texas System Limulus amoebocyte lysate assay and method of same
EP3483609B1 (en) 2017-11-09 2022-01-05 Andreas Buchberger Method and kit for sample preparation and endotoxin determination
CN109613228A (zh) * 2018-12-20 2019-04-12 湛江安度斯生物有限公司 一种减少或消除氢氧化铝佐剂干扰细菌内毒素检测的方法
CN111141909B (zh) * 2019-12-24 2023-05-30 南京健友生化制药股份有限公司 一种凝胶法检测硫代甘油中细菌内毒素的方法
AU2021251483A1 (en) * 2020-04-09 2022-12-15 DePuy Synthes Products, Inc. Quantifying endotoxin load in bacterial biofilms
CN112462015B (zh) * 2020-11-18 2022-07-12 海南倍特药业有限公司 一种氢溴酸瑞马唑仑细菌内毒素的检测方法
CN116242977A (zh) * 2022-12-14 2023-06-09 无锡药明生物技术股份有限公司 内毒素保留时间的估算方法及其应用
CN116609521B (zh) * 2023-01-17 2025-12-02 河北省药品医疗器械检验研究院(河北省化妆品检验研究中心) 重组乙型肝炎疫苗细菌内毒素快速检测试剂盒及其使用方法
CN116396916B (zh) * 2023-03-02 2023-12-01 广州普言生物科技有限公司 低内毒素大肠杆菌及低内毒素重组人源胶原蛋白大肠杆菌工程菌
CN117969228B (zh) * 2024-04-02 2024-05-28 成都翼泰生物科技有限公司 一种菌液稀释液、制备方法、参考品、试剂盒及其应用
CN120594816B (zh) * 2025-06-20 2025-11-25 北京全式金生物技术股份有限公司 一种重组c因子法检测内毒素的反应缓冲液及其应用

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Also Published As

Publication number Publication date
AU2016298598B2 (en) 2021-12-02
US20180188249A1 (en) 2018-07-05
IL256660A (en) 2018-02-28
AU2016298598A1 (en) 2018-01-18
CA2989960A1 (en) 2017-02-02
JP7164790B2 (ja) 2022-11-02
US20200240988A1 (en) 2020-07-30
EP3124976A1 (en) 2017-02-01
IL274576B (en) 2021-05-31
US10585097B2 (en) 2020-03-10
ES2699526T3 (es) 2019-02-11
WO2017017135A1 (en) 2017-02-02
TR201816369T4 (tr) 2018-11-21
CA2989960C (en) 2023-09-26
RS58167B1 (sr) 2019-03-29
IL256660B (en) 2020-06-30
KR20180030415A (ko) 2018-03-22
SI3124976T1 (sl) 2018-12-31
IL274576A (en) 2020-06-30
HRP20181954T1 (hr) 2019-01-25
CN108027374B (zh) 2020-07-07
BR112018001748A2 (en) 2018-09-18
MX2018000679A (es) 2018-11-29
JP2021051080A (ja) 2021-04-01
CN108027374A (zh) 2018-05-11
EP3124976B1 (en) 2018-09-12
PT3124976T (pt) 2018-11-20
US11360085B2 (en) 2022-06-14
DK3124976T3 (en) 2018-12-10
KR102630300B1 (ko) 2024-01-26
PL3124976T3 (pl) 2019-02-28
WO2017017135A8 (en) 2017-03-30
LT3124976T (lt) 2018-12-27
AR105497A1 (es) 2017-10-11
BR112018001748A8 (pt) 2023-04-25
CN111638346A (zh) 2020-09-08
JP2018529978A (ja) 2018-10-11
JP6800226B2 (ja) 2020-12-16

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