MX2017011489A - Detección de moléculas pequeñas mediante nanoporos a través de ensayos de competición. - Google Patents

Detección de moléculas pequeñas mediante nanoporos a través de ensayos de competición.

Info

Publication number
MX2017011489A
MX2017011489A MX2017011489A MX2017011489A MX2017011489A MX 2017011489 A MX2017011489 A MX 2017011489A MX 2017011489 A MX2017011489 A MX 2017011489A MX 2017011489 A MX2017011489 A MX 2017011489A MX 2017011489 A MX2017011489 A MX 2017011489A
Authority
MX
Mexico
Prior art keywords
detection
target
molecule
small molecules
nanopore
Prior art date
Application number
MX2017011489A
Other languages
English (en)
Inventor
J Morin Trevor
Original Assignee
Two Pore Guys Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Two Pore Guys Inc filed Critical Two Pore Guys Inc
Publication of MX2017011489A publication Critical patent/MX2017011489A/es

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3278—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction involving nanosized elements, e.g. nanogaps or nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Nanotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Se describen en la presente métodos y composiciones para la detección de moléculas diana pequeñas en una muestra mezclada al llevar a cabo un ensayo de competición entre la diana y un sustituto y detectar subsecuentemente los tipos complejos en un dispositivo de nanoporo.
MX2017011489A 2015-03-11 2016-03-11 Detección de moléculas pequeñas mediante nanoporos a través de ensayos de competición. MX2017011489A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562131693P 2015-03-11 2015-03-11
PCT/US2016/022210 WO2016145415A1 (en) 2015-03-11 2016-03-11 Nanopore detection of small molecules through competition assays

Publications (1)

Publication Number Publication Date
MX2017011489A true MX2017011489A (es) 2018-01-23

Family

ID=56878896

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017011489A MX2017011489A (es) 2015-03-11 2016-03-11 Detección de moléculas pequeñas mediante nanoporos a través de ensayos de competición.

Country Status (9)

Country Link
US (1) US10837954B2 (es)
EP (1) EP3268732A4 (es)
JP (1) JP6568949B2 (es)
KR (2) KR101945366B1 (es)
CN (1) CN107430107B (es)
CA (1) CA2978202A1 (es)
HK (1) HK1246395A1 (es)
MX (1) MX2017011489A (es)
WO (1) WO2016145415A1 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2973729A1 (en) * 2015-02-02 2016-08-11 Two Pore Guys, Inc. Labile linkers for biomarker detection
WO2017091724A1 (en) * 2015-11-23 2017-06-01 Two Pore Guys, Inc. Target modification for tracking and detection
CN107014791B (zh) * 2017-03-29 2019-10-29 郑州轻工业学院 一种荧光传感器及其制备方法和应用
CN108181358B (zh) * 2017-11-15 2020-02-11 华东理工大学 基于气单胞菌溶素纳米通道检测氨基酸的方法
DE102018210484A1 (de) 2018-06-27 2020-01-02 Robert Bosch Gmbh Sensor zum Detektieren von Ionen in einem Fluid sowie Verfahren zum Detektieren von Ionen in einem Fluid mit einem solchen Sensor
CN109239140B (zh) * 2018-08-16 2021-04-13 广东第二师范学院 一种纳米孔功能性控制方法及系统
CN109078628B (zh) * 2018-08-27 2021-06-11 西北大学 一种以苄胺为配基的高效疏水相互作用色谱介质、制备方法及其在蛋白质复性与纯化的应用
EP3847449A4 (en) * 2018-09-07 2022-08-31 Ontera Inc. DETECTION COMPOSITIONS, METHODS AND DEVICES FOR DETECTING MOLECULES USING A NANOPORE DEVICE
KR102127111B1 (ko) 2019-01-31 2020-06-26 경희대학교 산학협력단 나노포어의 제조 방법 및 이를 통해 제조된 나노포어 구조체
CN111999490B (zh) * 2019-12-19 2021-07-30 瑞芯智造(深圳)科技有限公司 一种检测样品体系中微量蛋白的方法

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003521695A (ja) * 2000-02-03 2003-07-15 リサーチ ディベロップメント ファンデーション 分子認識を識別信号に変換する情報伝達アプタマー
US6780584B1 (en) 2000-09-27 2004-08-24 Nanogen, Inc. Electronic systems and component devices for macroscopic and microscopic molecular biological reactions, analyses and diagnostics
US20030022150A1 (en) * 2001-07-24 2003-01-30 Sampson Jeffrey R. Methods for detecting a target molecule
AU2002361642A1 (en) 2001-11-16 2003-06-10 The University Of Tennessee Research Corporation Recombinant antibody fusion proteins and methods for detection of apoptotic cells
CN1742201A (zh) * 2002-11-14 2006-03-01 Qtl生物系统有限公司 使用荧光聚合物和猝灭剂－系链－配体生物缀合物的生物传感方法
US8346482B2 (en) 2003-08-22 2013-01-01 Fernandez Dennis S Integrated biosensor and simulation system for diagnosis and therapy
US20130071837A1 (en) * 2004-10-06 2013-03-21 Stephen N. Winters-Hilt Method and System for Characterizing or Identifying Molecules and Molecular Mixtures
EP2035831B1 (en) * 2006-06-30 2013-08-14 Applied Biosystems, LLC Methods of analyzing binding interactions
EP2076773B1 (en) * 2006-10-24 2010-12-29 Koninklijke Philips Electronics N.V. Detecting target molecules by luminescence
EP2205765B1 (en) * 2007-10-02 2012-09-12 President and Fellows of Harvard College Capture, recapture, and trapping of molecules with a nanopore
AU2011238582A1 (en) 2010-03-30 2012-10-25 Trustees Of Boston University Tools and method for nanopores unzipping-dependent nucleic acid sequencing
CN103328973B (zh) 2011-07-20 2015-04-01 加利福尼亚大学董事会 双孔装置
CN104379724B (zh) * 2012-01-31 2018-01-02 托莱多大学 用于分析物的检测和测量的方法和装置
ES2713424T3 (es) 2012-08-21 2019-05-21 Onity Inc Sistemas y métodos para la gestión de acceso a cerraduras utilizando señales inalámbricas
JP5951527B2 (ja) * 2013-03-07 2016-07-13 株式会社東芝 検体検出装置及び検出方法
ES2704902T3 (es) * 2013-05-06 2019-03-20 Two Pore Guys Inc Un método de detección de objetivos biológicos usando un nanoporo y un agente de unión a proteínas de fusión
US10048245B2 (en) * 2013-06-25 2018-08-14 Two Pore Guys, Inc. Multiplexed biomarker quantitation by nanopore analysis of biomarker-polymer complexes
WO2015031399A1 (en) * 2013-08-26 2015-03-05 Two Pore Guys, Inc. Molecule detection using boronic acid substituted probes
US9983191B2 (en) * 2015-03-11 2018-05-29 Two Pore Guys, Inc. Nanopore detection of small molecules through competition assays
WO2016196625A1 (en) 2015-06-02 2016-12-08 Nanopore Diagnostics, Llc Nucleic acid detection
WO2018093976A1 (en) 2016-11-16 2018-05-24 Nanopore Diagnostics, Llc Analysis of nucleic acids using probe with non-linear tag

Also Published As

Publication number Publication date
KR20170118229A (ko) 2017-10-24
US20180313814A1 (en) 2018-11-01
CN107430107A (zh) 2017-12-01
CN107430107B (zh) 2019-08-30
WO2016145415A1 (en) 2016-09-15
US10837954B2 (en) 2020-11-17
KR101945366B1 (ko) 2019-02-07
CA2978202A1 (en) 2016-09-15
KR20190012278A (ko) 2019-02-08
JP2018508023A (ja) 2018-03-22
EP3268732A1 (en) 2018-01-17
JP6568949B2 (ja) 2019-08-28
EP3268732A4 (en) 2018-07-25
HK1246395A1 (zh) 2018-09-07

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