MX355048B - Metodo de separacion para anticuerpos fucosilados. - Google Patents

Metodo de separacion para anticuerpos fucosilados.

Info

Publication number
MX355048B
MX355048B MX2014004604A MX2014004604A MX355048B MX 355048 B MX355048 B MX 355048B MX 2014004604 A MX2014004604 A MX 2014004604A MX 2014004604 A MX2014004604 A MX 2014004604A MX 355048 B MX355048 B MX 355048B
Authority
MX
Mexico
Prior art keywords
antibodies
separation method
fucosylated antibodies
separation
fucosylation
Prior art date
Application number
MX2014004604A
Other languages
English (en)
Other versions
MX2014004604A (es
Inventor
Umana Pablo
Freimoser-Grundschober Anne
Jaeger Christiane
Sondermann Peter
Original Assignee
Roche Glycart Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Glycart Ag filed Critical Roche Glycart Ag
Publication of MX2014004604A publication Critical patent/MX2014004604A/es
Publication of MX355048B publication Critical patent/MX355048B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70535—Fc-receptors, e.g. CD16, CD32, CD64 (CD2314/705F)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation

Abstract

La presente invención se refiere a un método para la separación de anticuerpos, específicamente anticuerpos que tienen diferentes grados de fucosilación. El método se basa en la afinidad de unión de los anticuerpos a los receptores Fc. La invención se refiere adicionalmente al uso de receptores Fc para la separación de anticuerpos que tienen diferentes grados de fucosilación.
MX2014004604A 2011-10-19 2012-10-16 Metodo de separacion para anticuerpos fucosilados. MX355048B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP11185798 2011-10-19
PCT/EP2012/070439 WO2013057078A1 (en) 2011-10-19 2012-10-16 Separation method for fucosylated antibodies

Publications (2)

Publication Number Publication Date
MX2014004604A MX2014004604A (es) 2014-05-27
MX355048B true MX355048B (es) 2018-04-03

Family

ID=47019025

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014004604A MX355048B (es) 2011-10-19 2012-10-16 Metodo de separacion para anticuerpos fucosilados.

Country Status (12)

Country Link
US (2) US9994610B2 (es)
EP (1) EP2768845B1 (es)
JP (2) JP6356605B2 (es)
KR (1) KR20140077178A (es)
CN (2) CN108314729A (es)
BR (1) BR112014009178A2 (es)
CA (1) CA2851053A1 (es)
ES (1) ES2619677T3 (es)
HK (2) HK1199272A1 (es)
MX (1) MX355048B (es)
RU (1) RU2650873C2 (es)
WO (1) WO2013057078A1 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
CN103555652A (zh) 2006-09-13 2014-02-05 Abbvie公司 细胞培养改良
TWI610936B (zh) 2008-10-20 2018-01-11 艾伯維有限公司 使用蛋白質ａ親和性層析進行抗體之分離及純化
CA2738499A1 (en) 2008-10-20 2010-04-29 Abbott Laboratories Viral inactivation during purification of antibodies
US10221210B2 (en) 2011-07-20 2019-03-05 Zepteon, Incorporated Polypeptide separation methods
JP6451119B2 (ja) * 2014-07-17 2019-01-16 東ソー株式会社 抗体依存性細胞傷害活性の強さに基づき抗体を分離する方法
EP3371202A1 (en) 2015-11-04 2018-09-12 Biogen MA Inc. Conjugation methods for modifying or immobilizing proteins
EP3638765A1 (en) * 2017-04-06 2020-04-22 Flodesign Sonics, Inc. Acoustic perfusion devices
JPWO2021010349A1 (es) * 2019-07-12 2021-01-21
GB202204016D0 (en) * 2022-03-22 2022-05-04 Ucl Business Plc Affinity chromatography ligands for antibody glycovariant separation

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3883899T3 (de) * 1987-03-18 1999-04-22 Sb2 Inc Geänderte antikörper.
US6410690B1 (en) * 1995-06-07 2002-06-25 Medarex, Inc. Therapeutic compounds comprised of anti-Fc receptor antibodies
DE69942021D1 (de) 1998-04-20 2010-04-01 Glycart Biotechnology Ag Glykosylierungs-engineering von antikörpern zur verbesserung der antikörperabhängigen zellvermittelten zytotoxizität
EP1006183A1 (en) * 1998-12-03 2000-06-07 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Recombinant soluble Fc receptors
EP1423510A4 (en) 2001-08-03 2005-06-01 Glycart Biotechnology Ag ANTIBODY GLYCOSYLATION VARIANTS WITH INCREASED CELL CYTOTOXICITY DEPENDENT OF ANTIBODIES
KR101498588B1 (ko) 2003-01-22 2015-03-05 로슈 글리카트 아게 융합 구성체와 Ｆｃ 수용체 결합 친화도 및 이펙터 기능이 증가된 항체를 생성하기 위한 이의 용도
RU2412200C2 (ru) * 2004-11-12 2011-02-20 Ксенкор, Инк. Fc-ВАРИАНТЫ С ИЗМЕНЕННЫМ СВЯЗЫВАНИЕМ С FcRn
US7699979B2 (en) * 2005-01-07 2010-04-20 Board Of Trustees Of The University Of Arkansas Separation system and efficient capture of contaminants using magnetic nanoparticles
ES2402650T3 (es) * 2005-06-17 2013-05-07 Janssen Alzheimer Immunotherapy Métodos de purificación de anticuerpos anti A beta
WO2010048313A2 (en) * 2008-10-22 2010-04-29 Biogen Idec Ma Inc. Recombinant fcrn and variants thereof for purification of fc-containing fusion proteins
EP2483693B1 (en) 2009-10-02 2014-12-03 Roche Glycart AG A-fucosylation detection in antibodies
US10221210B2 (en) * 2011-07-20 2019-03-05 Zepteon, Incorporated Polypeptide separation methods

Also Published As

Publication number Publication date
US9994610B2 (en) 2018-06-12
MX2014004604A (es) 2014-05-27
RU2650873C2 (ru) 2018-04-17
EP2768845B1 (en) 2017-01-18
CN103890002A (zh) 2014-06-25
HK1258766A1 (zh) 2019-11-22
HK1199272A1 (en) 2015-06-26
CA2851053A1 (en) 2013-04-25
KR20140077178A (ko) 2014-06-23
US20140255399A1 (en) 2014-09-11
JP2014530829A (ja) 2014-11-20
WO2013057078A1 (en) 2013-04-25
BR112014009178A2 (pt) 2018-09-04
US20180265544A1 (en) 2018-09-20
EP2768845A1 (en) 2014-08-27
RU2014118552A (ru) 2015-11-27
ES2619677T3 (es) 2017-06-26
JP2018184406A (ja) 2018-11-22
JP6356605B2 (ja) 2018-07-11
CN108314729A (zh) 2018-07-24

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