MX347754B - Desarrollo y uso de sondas fluorescentes de bilirrubina sin enlazar. - Google Patents
Desarrollo y uso de sondas fluorescentes de bilirrubina sin enlazar.Info
- Publication number
- MX347754B MX347754B MX2014002051A MX2014002051A MX347754B MX 347754 B MX347754 B MX 347754B MX 2014002051 A MX2014002051 A MX 2014002051A MX 2014002051 A MX2014002051 A MX 2014002051A MX 347754 B MX347754 B MX 347754B
- Authority
- MX
- Mexico
- Prior art keywords
- probes
- development
- fluorescent probes
- unbound bilirubin
- labeled
- Prior art date
Links
- BPYKTIZUTYGOLE-IFADSCNNSA-N Bilirubin Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(\C=C/3C(=C(C=C)C(=O)N\3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-IFADSCNNSA-N 0.000 title abstract 6
- 239000007850 fluorescent dye Substances 0.000 title 1
- 239000000523 sample Substances 0.000 abstract 4
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 abstract 2
- 235000018417 cysteine Nutrition 0.000 abstract 2
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 abstract 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 abstract 1
- 239000004472 Lysine Substances 0.000 abstract 1
- 239000012530 fluid Substances 0.000 abstract 1
- 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
- G01N33/728—Bilirubin; including biliverdin
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/145555—Hetero-N
- Y10T436/146666—Bile pigment
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Organic Chemistry (AREA)
- Endocrinology (AREA)
- Genetics & Genomics (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Optics & Photonics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
La presente invención se refiere a una composición que comprende (a) una sonda que se une a la bilirrubina pero no se une significantemente al ácido graso que comprende una muteína de proteína enlazadora de lípidos intracelulares (iLBP) etiquetada con un primer fluoróforo, en donde la muteína de iLBP corresponde a SEQ ID NO: 3 comprende una o más sustituciones de aminoácidos en posiciones seleccionadas del grupo que consiste en las posiciones 14, 18, 23, 25, 27, 31, 36, 38, 55, 60, 72, 73, 74, 78, 102, 104, 106, 115 y 117, y en donde el primer fluoróforo está unido a un residuo de lisina, el grupo amino de término N de la iLBP, o una substitución de cisteína, y (b) un segundo fluoróforo, en donde el segundo fluoróforo está libre en la solución o unido a una proteína que no se une a la bilurrubina, y en donde el primer fluoróforo y el segundo fluoróforo son capaces de la excitación en la misma longitud de onda, y en donde la longitud de onda de emisión del primer fluoróforo y el segundo fluoróforo es diferente, y en donde el segundo fluróforo no cambia su emisión en respuesta a la bilurribuna que se une a la muteína de iLBP.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161527849P | 2011-08-26 | 2011-08-26 | |
| PCT/US2012/052395 WO2013032953A2 (en) | 2011-08-26 | 2012-08-24 | Development and use of fluorescent probes of unbound bilirubin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2014002051A MX2014002051A (es) | 2014-09-12 |
| MX347754B true MX347754B (es) | 2017-05-11 |
Family
ID=47757131
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2014002051A MX347754B (es) | 2011-08-26 | 2012-08-24 | Desarrollo y uso de sondas fluorescentes de bilirrubina sin enlazar. |
| MX2019010563A MX380526B (es) | 2011-08-26 | 2012-08-24 | Desarrollo y uso de sondas fluorescentes de bilirrubina sin enlazar |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2019010563A MX380526B (es) | 2011-08-26 | 2012-08-24 | Desarrollo y uso de sondas fluorescentes de bilirrubina sin enlazar |
Country Status (12)
| Country | Link |
|---|---|
| US (3) | US9529003B2 (es) |
| EP (2) | EP2748615B1 (es) |
| JP (3) | JP6104913B2 (es) |
| CN (2) | CN104220877B (es) |
| AU (3) | AU2012300319B8 (es) |
| CA (1) | CA2846106C (es) |
| CY (1) | CY1122022T1 (es) |
| DK (1) | DK2748615T3 (es) |
| ES (1) | ES2733974T3 (es) |
| MX (2) | MX347754B (es) |
| PT (1) | PT2748615T (es) |
| WO (1) | WO2013032953A2 (es) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10065996B2 (en) * | 2012-11-16 | 2018-09-04 | Iba Gmbh | Streptavidin muteins and methods of using them |
| US9957308B2 (en) * | 2013-02-28 | 2018-05-01 | Riken | Method for detecting bilirubin using a fluorescent protein |
| EP3077827B1 (en) | 2013-12-03 | 2023-11-01 | Kleinfeld, Alan M. | Determination of the unbound free fatty acid profiles in biological and industrial specimens |
| US20160146838A1 (en) * | 2014-11-20 | 2016-05-26 | Jean Ronald Brisard | System and method for ebola detection |
| CN106872379B (zh) * | 2015-05-16 | 2019-08-27 | 深圳市启蒙智慧医疗科技有限公司 | 一种尿液成分测量分析仪 |
| JP7228163B2 (ja) * | 2017-04-11 | 2023-02-24 | 国立研究開発法人産業技術総合研究所 | レクチンの固定化方法 |
| JP7166429B2 (ja) * | 2018-04-03 | 2022-11-07 | ネオメトリックス ディーエックス， インコーポレイテッド | ビリルビンのｉｎ ｖｉｖｏ毒性レベルを評価し、ビリルビン神経毒性の増大したリスクを診断するための、方法およびデバイス |
| CN111334079A (zh) * | 2018-12-18 | 2020-06-26 | 中国科学院大连化学物理研究所 | 一种全光谱高亮度、高稳定性荧光染料及其合成和应用 |
| MX2022002328A (es) * | 2019-08-30 | 2022-06-02 | Alan Marc Kleinfeld | Metodos de un solo paso, kits, y sistemas para la medicion de concentraciones de bilirrubina no enlazada en fluidos biologicos. |
| CN111239094B (zh) * | 2020-03-13 | 2022-08-26 | 河南中医药大学 | 一种碱性磷酸酶的灵敏检测方法 |
| CN113238041B (zh) * | 2021-01-13 | 2024-01-16 | 军事科学院军事医学研究院环境医学与作业医学研究所 | 一种基于β-环糊精修饰的免疫生物条形码增敏探针以及快速检测阿特拉津的方法 |
| US11908583B2 (en) * | 2022-05-02 | 2024-02-20 | Kpn Innovations, Llc. | Apparatus and method for determining toxic load quantifiers |
| CN120112542A (zh) * | 2023-09-27 | 2025-06-06 | 京东方科技集团股份有限公司 | 针对亨廷顿舞蹈症的早期诊断的多肽探针和试剂盒 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5470714A (en) | 1989-12-12 | 1995-11-28 | Lidak Pharmaceuticals | One-step free fatty acid determination method |
| US6444432B1 (en) | 1997-06-13 | 2002-09-03 | Alan M. Kleinfeld | Method of detection of cardiac ischemia using fatty acid binding protein |
| ES2266224T3 (es) | 2000-08-15 | 2007-03-01 | Discerna Limited | Series de proteinas funcionales. |
| AU2005227312B2 (en) * | 2004-03-22 | 2011-04-07 | Ffa Sciences, Llp | Development and use of fluorescent probes of unbound analytes |
| US20060257938A1 (en) * | 2005-05-10 | 2006-11-16 | Kleinfeld Alan M | Method of screening for drugs that block ligand binding to a lipid binding protein |
| WO2008060841A2 (en) | 2006-10-27 | 2008-05-22 | Ffa Sciences, Llc | Use of probes for unbound metabolites |
| WO2009092047A1 (en) * | 2008-01-18 | 2009-07-23 | Ffa Sciences, Llc | Development and use of cysteine - labeled fluorescent probes of unbound analytes |
-
2012
- 2012-08-24 MX MX2014002051A patent/MX347754B/es active IP Right Grant
- 2012-08-24 WO PCT/US2012/052395 patent/WO2013032953A2/en not_active Ceased
- 2012-08-24 ES ES12827317T patent/ES2733974T3/es active Active
- 2012-08-24 CN CN201280041789.4A patent/CN104220877B/zh active Active
- 2012-08-24 DK DK12827317.4T patent/DK2748615T3/da active
- 2012-08-24 AU AU2012300319A patent/AU2012300319B8/en active Active
- 2012-08-24 JP JP2014527346A patent/JP6104913B2/ja active Active
- 2012-08-24 EP EP12827317.4A patent/EP2748615B1/en active Active
- 2012-08-24 CA CA2846106A patent/CA2846106C/en active Active
- 2012-08-24 US US14/238,144 patent/US9529003B2/en active Active
- 2012-08-24 EP EP19159898.6A patent/EP3598139A1/en active Pending
- 2012-08-24 MX MX2019010563A patent/MX380526B/es unknown
- 2012-08-24 PT PT12827317T patent/PT2748615T/pt unknown
- 2012-08-24 CN CN201711257192.4A patent/CN108051584B/zh active Active
-
2016
- 2016-11-17 US US15/354,188 patent/US10295551B2/en active Active
-
2017
- 2017-03-01 JP JP2017038572A patent/JP6694403B2/ja not_active Expired - Fee Related
- 2017-07-25 AU AU2017208236A patent/AU2017208236B2/en active Active
-
2019
- 2019-03-11 JP JP2019043964A patent/JP6847141B2/ja active Active
- 2019-03-27 US US16/366,143 patent/US11029320B2/en active Active
- 2019-07-16 AU AU2019206014A patent/AU2019206014B2/en active Active
- 2019-07-29 CY CY20191100805T patent/CY1122022T1/el unknown
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |