MX2019010563A - Desarrollo y uso de sondas fluorescentes de bilirrubina sin enlazar. - Google Patents

Desarrollo y uso de sondas fluorescentes de bilirrubina sin enlazar.

Info

Publication number
MX2019010563A
MX2019010563A MX2019010563A MX2019010563A MX2019010563A MX 2019010563 A MX2019010563 A MX 2019010563A MX 2019010563 A MX2019010563 A MX 2019010563A MX 2019010563 A MX2019010563 A MX 2019010563A MX 2019010563 A MX2019010563 A MX 2019010563A
Authority
MX
Mexico
Prior art keywords
probes
development
fluorescent probes
unbound bilirubin
labeled
Prior art date
Application number
MX2019010563A
Other languages
English (en)
Inventor
Zhu Baolong
Marc Kleinfeld Alan
Henry Huber Andrew
Kwan Thomas
Patrick Kampf James
Original Assignee
Marc Kleinfeld Alan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Marc Kleinfeld Alan filed Critical Marc Kleinfeld Alan
Publication of MX2019010563A publication Critical patent/MX2019010563A/es

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/728—Bilirubin; including biliverdin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/145555—Hetero-N
    • Y10T436/146666—Bile pigment

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Organic Chemistry (AREA)
  • Endocrinology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Se describe la identificación y uso de proteínas marcadas fluorescentemente y que sufren un cambio en el índice de fluorescencia después del enlace a bilirrubina. Se revelan sondas que son marcadas en un residuo de cisteína o lisina y también sondas marcadas tanto en cisteína como en lisina con dos fluoróforos diferentes. Estas sondas son útiles para la determinación de niveles de bilirrubina sin enlazar en una muestra del fluido.
MX2019010563A 2011-08-26 2014-02-20 Desarrollo y uso de sondas fluorescentes de bilirrubina sin enlazar. MX2019010563A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201161527849P 2011-08-26 2011-08-26

Publications (1)

Publication Number Publication Date
MX2019010563A true MX2019010563A (es) 2019-10-24

Family

ID=47757131

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2014002051A MX347754B (es) 2011-08-26 2012-08-24 Desarrollo y uso de sondas fluorescentes de bilirrubina sin enlazar.
MX2019010563A MX2019010563A (es) 2011-08-26 2014-02-20 Desarrollo y uso de sondas fluorescentes de bilirrubina sin enlazar.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2014002051A MX347754B (es) 2011-08-26 2012-08-24 Desarrollo y uso de sondas fluorescentes de bilirrubina sin enlazar.

Country Status (12)

Country Link
US (3) US9529003B2 (es)
EP (2) EP3598139A1 (es)
JP (3) JP6104913B2 (es)
CN (2) CN104220877B (es)
AU (3) AU2012300319B8 (es)
CA (1) CA2846106C (es)
CY (1) CY1122022T1 (es)
DK (1) DK2748615T3 (es)
ES (1) ES2733974T3 (es)
MX (2) MX347754B (es)
PT (1) PT2748615T (es)
WO (1) WO2013032953A2 (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2891820A1 (en) * 2012-11-16 2014-05-22 Iba Gmbh Streptavidin muteins and methods of using them
WO2014133158A1 (ja) * 2013-02-28 2014-09-04 独立行政法人理化学研究所 蛍光特性を示す新規なポリペプチド、およびその利用
EP3077827B1 (en) 2013-12-03 2023-11-01 Kleinfeld, Alan M. Determination of the unbound free fatty acid profiles in biological and industrial specimens
US20160146838A1 (en) * 2014-11-20 2016-05-26 Jean Ronald Brisard System and method for ebola detection
CN104819981B (zh) * 2015-05-16 2017-11-21 山东耀华医疗器械股份有限公司 一种尿液成分测量仪
US11078254B2 (en) * 2017-04-11 2021-08-03 National Institute Of Advanced Industrial Science And Technology Method for immobilizing lectin
JP7166429B2 (ja) * 2018-04-03 2022-11-07 ネオメトリックス ディーエックス， インコーポレイテッド ビリルビンのｉｎ ｖｉｖｏ毒性レベルを評価し、ビリルビン神経毒性の増大したリスクを診断するための、方法およびデバイス
CN111334079A (zh) * 2018-12-18 2020-06-26 中国科学院大连化学物理研究所 一种全光谱高亮度、高稳定性荧光染料及其合成和应用
CA3152537A1 (en) * 2019-08-30 2021-03-04 Alan Marc Kleinfeld One step methods, kits, and systems for the measurement of concentrations of unbound bilirubin in biological fluids
CN111239094B (zh) * 2020-03-13 2022-08-26 河南中医药大学 一种碱性磷酸酶的灵敏检测方法
CN113238041B (zh) * 2021-01-13 2024-01-16 军事科学院军事医学研究院环境医学与作业医学研究所 一种基于β-环糊精修饰的免疫生物条形码增敏探针以及快速检测阿特拉津的方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5470714A (en) 1989-12-12 1995-11-28 Lidak Pharmaceuticals One-step free fatty acid determination method
US6444432B1 (en) 1997-06-13 2002-09-03 Alan M. Kleinfeld Method of detection of cardiac ischemia using fatty acid binding protein
DK1309861T3 (da) 2000-08-15 2006-10-23 Discerna Ltd Funktionelle protein-arrays
AU2005227312B2 (en) * 2004-03-22 2011-04-07 Ffa Sciences, Llp Development and use of fluorescent probes of unbound analytes
US20060257938A1 (en) * 2005-05-10 2006-11-16 Kleinfeld Alan M Method of screening for drugs that block ligand binding to a lipid binding protein
WO2008060841A2 (en) 2006-10-27 2008-05-22 Ffa Sciences, Llc Use of probes for unbound metabolites
WO2009092047A1 (en) 2008-01-18 2009-07-23 Ffa Sciences, Llc Development and use of cysteine - labeled fluorescent probes of unbound analytes

Also Published As

Publication number Publication date
AU2019206014B2 (en) 2021-10-07
JP2014526055A (ja) 2014-10-02
AU2012300319A1 (en) 2014-02-27
JP2019146569A (ja) 2019-09-05
US20150044692A1 (en) 2015-02-12
CN104220877B (zh) 2018-05-15
DK2748615T3 (da) 2019-07-29
EP2748615B1 (en) 2019-05-01
ES2733974T3 (es) 2019-12-03
JP6694403B2 (ja) 2020-05-13
AU2019206014A1 (en) 2019-09-26
AU2012300319A8 (en) 2017-07-27
CN108051584B (zh) 2021-02-12
CN104220877A (zh) 2014-12-17
PT2748615T (pt) 2019-07-22
US20170097366A1 (en) 2017-04-06
JP2017104128A (ja) 2017-06-15
CN108051584A (zh) 2018-05-18
AU2012300319B8 (en) 2017-07-27
EP2748615A2 (en) 2014-07-02
EP2748615A4 (en) 2015-07-08
AU2012300319B2 (en) 2017-07-13
AU2017208236B2 (en) 2019-04-18
MX2014002051A (es) 2014-09-12
JP6104913B2 (ja) 2017-03-29
EP3598139A1 (en) 2020-01-22
CA2846106A1 (en) 2013-03-07
WO2013032953A8 (en) 2014-10-09
AU2012300319A2 (en) 2015-06-04
WO2013032953A3 (en) 2013-08-08
US20190212346A1 (en) 2019-07-11
CA2846106C (en) 2020-09-01
US11029320B2 (en) 2021-06-08
AU2017208236A1 (en) 2017-08-10
MX347754B (es) 2017-05-11
CY1122022T1 (el) 2020-10-14
US10295551B2 (en) 2019-05-21
US9529003B2 (en) 2016-12-27
JP6847141B2 (ja) 2021-03-24
WO2013032953A2 (en) 2013-03-07

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