WO2013032953A3 - Development and use of fluorescent probes of unbound bilirubin - Google Patents

Development and use of fluorescent probes of unbound bilirubin Download PDF

Info

Publication number
WO2013032953A3
WO2013032953A3 PCT/US2012/052395 US2012052395W WO2013032953A3 WO 2013032953 A3 WO2013032953 A3 WO 2013032953A3 US 2012052395 W US2012052395 W US 2012052395W WO 2013032953 A3 WO2013032953 A3 WO 2013032953A3
Authority
WO
WIPO (PCT)
Prior art keywords
probes
development
fluorescent probes
unbound bilirubin
labeled
Prior art date
Application number
PCT/US2012/052395
Other languages
French (fr)
Other versions
WO2013032953A2 (en
WO2013032953A8 (en
Inventor
Alan Marc Kleinfeld
Andrew Henry Huber
James Patrick Kampf
Thomas Kwan
Baolong Zhu
Original Assignee
Alan Marc Kleinfeld
Andrew Henry Huber
James Patrick Kampf
Thomas Kwan
Baolong Zhu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to EP12827317.4A priority Critical patent/EP2748615B1/en
Application filed by Alan Marc Kleinfeld, Andrew Henry Huber, James Patrick Kampf, Thomas Kwan, Baolong Zhu filed Critical Alan Marc Kleinfeld
Priority to DK12827317.4T priority patent/DK2748615T3/en
Priority to JP2014527346A priority patent/JP6104913B2/en
Priority to CN201280041789.4A priority patent/CN104220877B/en
Priority to AU2012300319A priority patent/AU2012300319B8/en
Priority to US14/238,144 priority patent/US9529003B2/en
Priority to EP19159898.6A priority patent/EP3598139A1/en
Priority to MX2014002051A priority patent/MX347754B/en
Priority to CA2846106A priority patent/CA2846106C/en
Priority to ES12827317T priority patent/ES2733974T3/en
Publication of WO2013032953A2 publication Critical patent/WO2013032953A2/en
Publication of WO2013032953A3 publication Critical patent/WO2013032953A3/en
Publication of WO2013032953A8 publication Critical patent/WO2013032953A8/en
Priority to US15/354,188 priority patent/US10295551B2/en
Priority to AU2017208236A priority patent/AU2017208236B2/en
Priority to US16/366,143 priority patent/US11029320B2/en
Priority to AU2019206014A priority patent/AU2019206014B2/en
Priority to CY20191100805T priority patent/CY1122022T1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/728—Bilirubin; including biliverdin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/145555—Hetero-N
    • Y10T436/146666—Bile pigment

Abstract

Identification and use of proteins fluorescently labeled and that undergo a change in fluorescence index upon binding bilirubin are described. Probes are disclosed which are labeled at a cysteine or lysine residue and also probes labeled at both cysteine and lysine with two different fluorophores. These probes are useful for determination of unbound bilirubin levels in a fluid sample.
PCT/US2012/052395 2011-08-26 2012-08-24 Development and use of fluorescent probes of unbound bilirubin WO2013032953A2 (en)

Priority Applications (15)

Application Number Priority Date Filing Date Title
MX2014002051A MX347754B (en) 2011-08-26 2012-08-24 Development and use of fluorescent probes of unbound bilirubin.
EP19159898.6A EP3598139A1 (en) 2011-08-26 2012-08-24 Development and use of fluorescent probes of unbound bilirubin
DK12827317.4T DK2748615T3 (en) 2011-08-26 2012-08-24 Development and use of unbound bilirubin fluorescent probes
JP2014527346A JP6104913B2 (en) 2011-08-26 2012-08-24 Development and use of fluorescent probes for unbound bilirubin
CN201280041789.4A CN104220877B (en) 2011-08-26 2012-08-24 The development and application of the fluorescence probe of unconjugated bilirubin
AU2012300319A AU2012300319B8 (en) 2011-08-26 2012-08-24 Development and use of fluorescent probes of unbound bilirubin
US14/238,144 US9529003B2 (en) 2011-08-26 2012-08-24 Development and use of fluorescent probes of unbound bilirubin
EP12827317.4A EP2748615B1 (en) 2011-08-26 2012-08-24 Development and use of fluorescent probes of unbound bilirubin
CA2846106A CA2846106C (en) 2011-08-26 2012-08-24 Development and use of fluorescent probes of unbound bilirubin
ES12827317T ES2733974T3 (en) 2011-08-26 2012-08-24 Development and use of unbound bilirubin fluorescent probes
US15/354,188 US10295551B2 (en) 2011-08-26 2016-11-17 Development and use of fluorescent probes of unbound bilirubin
AU2017208236A AU2017208236B2 (en) 2011-08-26 2017-07-25 Development and use of fluorescent probes of unbound bilirubin
US16/366,143 US11029320B2 (en) 2011-08-26 2019-03-27 Development and use of fluorescent probes of unbound bilirubin
AU2019206014A AU2019206014B2 (en) 2011-08-26 2019-07-16 Development and use of fluorescent probes of unbound bilirubin
CY20191100805T CY1122022T1 (en) 2011-08-26 2019-07-29 DEVELOPMENT AND USE OF FLUORESCENT CHOLERYTHRIN BINDING DETECTORS

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161527849P 2011-08-26 2011-08-26
US61/527,849 2011-08-26

Related Child Applications (3)

Application Number Title Priority Date Filing Date
EP19159898.6A Previously-Filed-Application EP3598139A1 (en) 2011-08-26 2012-08-24 Development and use of fluorescent probes of unbound bilirubin
US14/238,144 A-371-Of-International US9529003B2 (en) 2011-08-26 2012-08-24 Development and use of fluorescent probes of unbound bilirubin
US15/354,188 Continuation US10295551B2 (en) 2011-08-26 2016-11-17 Development and use of fluorescent probes of unbound bilirubin

Publications (3)

Publication Number Publication Date
WO2013032953A2 WO2013032953A2 (en) 2013-03-07
WO2013032953A3 true WO2013032953A3 (en) 2013-08-08
WO2013032953A8 WO2013032953A8 (en) 2014-10-09

Family

ID=47757131

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/052395 WO2013032953A2 (en) 2011-08-26 2012-08-24 Development and use of fluorescent probes of unbound bilirubin

Country Status (12)

Country Link
US (3) US9529003B2 (en)
EP (2) EP3598139A1 (en)
JP (3) JP6104913B2 (en)
CN (2) CN104220877B (en)
AU (3) AU2012300319B8 (en)
CA (1) CA2846106C (en)
CY (1) CY1122022T1 (en)
DK (1) DK2748615T3 (en)
ES (1) ES2733974T3 (en)
MX (2) MX347754B (en)
PT (1) PT2748615T (en)
WO (1) WO2013032953A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2891820A1 (en) * 2012-11-16 2014-05-22 Iba Gmbh Streptavidin muteins and methods of using them
US9957308B2 (en) * 2013-02-28 2018-05-01 Riken Method for detecting bilirubin using a fluorescent protein
EP3077827B1 (en) 2013-12-03 2023-11-01 Kleinfeld, Alan M. Determination of the unbound free fatty acid profiles in biological and industrial specimens
US20160146838A1 (en) * 2014-11-20 2016-05-26 Jean Ronald Brisard System and method for ebola detection
CN104819981B (en) * 2015-05-16 2017-11-21 山东耀华医疗器械股份有限公司 A kind of urinary fractions measuring instrument
WO2018190357A1 (en) * 2017-04-11 2018-10-18 国立研究開発法人産業技術総合研究所 Method for immobilizing lectin
WO2019195317A1 (en) * 2018-04-03 2019-10-10 Neometrix Dx Methods and devices for assessing in vivo toxic levels of bilirubin and diagnosing increased risk of bilirubin neurotoxicity
CN111334079A (en) * 2018-12-18 2020-06-26 中国科学院大连化学物理研究所 Full-spectrum high-brightness and high-stability fluorescent dye and synthesis and application thereof
KR20220104676A (en) * 2019-08-30 2022-07-26 알랜 클레인펠드 One Step Methods, Kits and Systems for Determination of Concentrations of Unbound Bilirubin in Biological Fluids
CN111239094B (en) * 2020-03-13 2022-08-26 河南中医药大学 Sensitive detection method of alkaline phosphatase
CN113238041B (en) * 2021-01-13 2024-01-16 军事科学院军事医学研究院环境医学与作业医学研究所 Beta-cyclodextrin modification-based immune biological bar code sensitization probe and method for rapidly detecting atrazine

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050244864A1 (en) * 2004-03-22 2005-11-03 Kleinfeld Alan M Development and use of fluorescent probes of unbound analytes
US20100298162A1 (en) * 2008-01-18 2010-11-25 Ffa Sciences, Llc Development and use of cysteine-labeled fluorescent probes of unbound analytes

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5470714A (en) 1989-12-12 1995-11-28 Lidak Pharmaceuticals One-step free fatty acid determination method
US6444432B1 (en) 1997-06-13 2002-09-03 Alan M. Kleinfeld Method of detection of cardiac ischemia using fatty acid binding protein
ATE330224T1 (en) 2000-08-15 2006-07-15 Discerna Ltd FUNCTIONAL PROTEIN ARRAY
US20060257938A1 (en) * 2005-05-10 2006-11-16 Kleinfeld Alan M Method of screening for drugs that block ligand binding to a lipid binding protein
WO2008060841A2 (en) * 2006-10-27 2008-05-22 Ffa Sciences, Llc Use of probes for unbound metabolites

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050244864A1 (en) * 2004-03-22 2005-11-03 Kleinfeld Alan M Development and use of fluorescent probes of unbound analytes
US20100298162A1 (en) * 2008-01-18 2010-11-25 Ffa Sciences, Llc Development and use of cysteine-labeled fluorescent probes of unbound analytes

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
HUBER, A. H. ET AL.: "Fatty acid-specific fluorescent probes and their use in resolving mixtures of unbound free fatty acids in equilibrium with albumin", BIOCHEMISTRY, vol. 45, no. 48, 5 December 2006 (2006-12-05), pages 14263 - 14274, XP055081885 *

Also Published As

Publication number Publication date
AU2019206014A1 (en) 2019-09-26
CA2846106A1 (en) 2013-03-07
MX2019010563A (en) 2019-10-24
AU2017208236A1 (en) 2017-08-10
DK2748615T3 (en) 2019-07-29
PT2748615T (en) 2019-07-22
JP6104913B2 (en) 2017-03-29
EP3598139A1 (en) 2020-01-22
EP2748615B1 (en) 2019-05-01
CA2846106C (en) 2020-09-01
CN104220877A (en) 2014-12-17
CN104220877B (en) 2018-05-15
ES2733974T3 (en) 2019-12-03
EP2748615A4 (en) 2015-07-08
US20190212346A1 (en) 2019-07-11
US11029320B2 (en) 2021-06-08
JP6694403B2 (en) 2020-05-13
AU2012300319A8 (en) 2017-07-27
MX2014002051A (en) 2014-09-12
EP2748615A2 (en) 2014-07-02
JP6847141B2 (en) 2021-03-24
AU2012300319B8 (en) 2017-07-27
CN108051584B (en) 2021-02-12
WO2013032953A2 (en) 2013-03-07
US10295551B2 (en) 2019-05-21
CN108051584A (en) 2018-05-18
AU2012300319B2 (en) 2017-07-13
AU2019206014B2 (en) 2021-10-07
JP2019146569A (en) 2019-09-05
US20170097366A1 (en) 2017-04-06
AU2012300319A2 (en) 2015-06-04
MX347754B (en) 2017-05-11
WO2013032953A8 (en) 2014-10-09
US9529003B2 (en) 2016-12-27
CY1122022T1 (en) 2020-10-14
AU2012300319A1 (en) 2014-02-27
JP2017104128A (en) 2017-06-15
JP2014526055A (en) 2014-10-02
AU2017208236B2 (en) 2019-04-18
US20150044692A1 (en) 2015-02-12

Similar Documents

Publication Publication Date Title
WO2013032953A3 (en) Development and use of fluorescent probes of unbound bilirubin
WO2013190075A3 (en) Specific biomarkers for hepatocellular carcinoma (hcc)
HK1208071A1 (en) Multi-application approach for photometric determination of an analyte in a fluid sample on an automated analyzer
EA201600085A1 (en) KIT FOR DETECTION OF SOYO SYNT SYNT0H2 EVENT
WO2010065568A3 (en) METHODS AND ASSAYS FOR MEASURING p95 AND/OR p95 IN A SAMPLE AND ANTIBODIES SPECIFIC FOR p95
BRPI1013688A8 (en) IL-17 BINDING PROTEINS.
MX367366B (en) Compositions and methods for diagnosing thyroid tumors.
GB0606776D0 (en) Predictive biomarkers for chronic allograft nephropathy
WO2012102535A3 (en) Composition for detecting proteins containing tyrosine oxide-coupled biomaterial
WO2008125747A3 (en) Fluorescent emulsions for optical imaging
WO2012094580A3 (en) Compounds that modulate oxidative stress
EA201690731A1 (en) ANALYSIS OF IGFBP7, HAVING IMPROVED CHARACTERISTICS IN BIOLOGICAL SPECIMENS
DE112009003237A5 (en) BIOPSY INSTRUMENT FOR ENRICHING SAMPLE MATERIAL
CL2013000813A1 (en) Immunochromatographic device comprising a membrane having an antibody capable of binding to a z-aat protein present in a sample of a piz gene carrier; method for determining a carrier of the piz gene; method to diagnose a condition or disease associated with aat deficiency; immunochromatography kits
BRPI0907864A2 (en) Identification of antigen or ligand-specific binding proteins
DK2183599T3 (en) High-sensitivity immunoassays and kits for the determination of peptides and proteins of biological interest
ATE514090T1 (en) USE OF THE PROTEIN SATB2 AS A MARKER TO DIFFERENTIATE COLORECTAL CARCINOMA FROM OTHER CANCER DISEASES
BR112012019475A8 (en) use of an anti-cd44 monoclonal antibody, anti-cd44 monoclonal antibody, humanized anti-cd44 antibody and kit for detecting hnscc in a sample
BR112014031958A2 (en) bag3 as tissue marker and biochemical serum
PE20160657A1 (en) COMPOSITIONS AND METHODS TO IDENTIFY A RISK OF CANCER IN A SUBJECT
EE200600030A (en) Protein kinase fluorescence probe
BRPI1009460A2 (en) antibody binding domain, antibody, use of a binding domain or antibody, method for detecting the presence of cancer cells in a sample.
WO2012145516A3 (en) Methods and kits for quantitative determination of total organic acid content in a coolant
WO2014160273A3 (en) Protein standard
WO2014151290A3 (en) Assay for predictive biomarkers

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 12827317

Country of ref document: EP

Kind code of ref document: A2

WWE Wipo information: entry into national phase

Ref document number: 14238144

Country of ref document: US

Ref document number: 2012827317

Country of ref document: EP

ENP Entry into the national phase

Ref document number: 2846106

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: MX/A/2014/002051

Country of ref document: MX

ENP Entry into the national phase

Ref document number: 2014527346

Country of ref document: JP

Kind code of ref document: A

NENP Non-entry into the national phase

Ref country code: DE

ENP Entry into the national phase

Ref document number: 2012300319

Country of ref document: AU

Date of ref document: 20120824

Kind code of ref document: A