MX346558B - Hongos filamentosos que tienen un fenotipo de viscosidad alterado. - Google Patents

Hongos filamentosos que tienen un fenotipo de viscosidad alterado.

Info

Publication number
MX346558B
MX346558B MX2013002087A MX2013002087A MX346558B MX 346558 B MX346558 B MX 346558B MX 2013002087 A MX2013002087 A MX 2013002087A MX 2013002087 A MX2013002087 A MX 2013002087A MX 346558 B MX346558 B MX 346558B
Authority
MX
Mexico
Prior art keywords
filamentous fungi
viscosity phenotype
altered viscosity
altered
phenotype
Prior art date
Application number
MX2013002087A
Other languages
English (en)
Other versions
MX2013002087A (es
Inventor
C Dodge Timothy
Ward Michael
Virag Aleksandra
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Publication of MX2013002087A publication Critical patent/MX2013002087A/es
Publication of MX346558B publication Critical patent/MX346558B/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invención se refiere a una cepa variante de hongo filamentoso derivada de una cepa parental, caracterizada porque comprende una alteración genética que provoca que las células de la cepea variante produzcan una cantidad reducida de proteína funcional Sfb3 en comparación con las células de la cepa parental, en donde la alteración genética es una modificación del gen sfb3, en donde las células de la cepa variante producen, durante la fermentación aeróbica en cultivo sumergido, un caldo celular que: (i) requiere una cantidad alterada de agitación para mantener un contenido de oxígeno disuelto preseleccionado en comparación con las células de la cepa parental, y/o (ii) mantiene un contenido de oxígeno disuelto alterado en una cantidad preseleccionada de agitación, en comparación con las células de la cepa parental.
MX2013002087A 2010-08-25 2011-08-25 Hongos filamentosos que tienen un fenotipo de viscosidad alterado. MX346558B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37703010P 2010-08-25 2010-08-25
PCT/US2011/049164 WO2012027580A1 (en) 2010-08-25 2011-08-25 Filamentous fungi having an altered viscosity phenotype

Publications (2)

Publication Number Publication Date
MX2013002087A MX2013002087A (es) 2013-05-09
MX346558B true MX346558B (es) 2017-03-24

Family

ID=44720107

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013002087A MX346558B (es) 2010-08-25 2011-08-25 Hongos filamentosos que tienen un fenotipo de viscosidad alterado.

Country Status (14)

Country Link
US (1) US10266832B2 (es)
EP (1) EP2609200B1 (es)
JP (1) JP5707494B2 (es)
KR (1) KR102004003B1 (es)
CN (1) CN103097536B (es)
AU (1) AU2011293263B2 (es)
BR (1) BR112013004419A2 (es)
CA (1) CA2808777C (es)
DK (1) DK2609200T3 (es)
MX (1) MX346558B (es)
MY (1) MY160960A (es)
RU (1) RU2608639C2 (es)
WO (1) WO2012027580A1 (es)
ZA (1) ZA201208720B (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3000870T3 (en) * 2011-04-22 2018-04-30 Danisco Us Inc FILAMENTOUS FUNGI WITH CHANGED VISCOSITY PHENOTYPE
EP2699667B1 (en) * 2011-04-22 2016-04-13 Danisco US Inc. Filamentous fungi having an altered viscosity phenotype
US11015175B2 (en) * 2012-11-09 2021-05-25 Tohoku University Method for manufacturing useful substance in which high-density cultured strain of filamentous fungi is used
BR112018069854A2 (pt) * 2016-03-31 2019-01-29 Toray Industries métodos para produzir proteína, xilo-oligossacarídeos e glicose, método para suprimir a diminuição na saturação de oxigênio dissolvido ao cultivar um fungo e composição de celulase
EP3755710A1 (en) 2018-04-24 2020-12-30 Danisco US Inc. Filamentous fungal strains comprising reduced viscosity phenotypes
EP3831929A4 (en) * 2018-07-30 2022-04-27 Toray Industries, Inc. MUTATION OF TRICHODERMA THREAD FUNGUS AND METHOD OF PRODUCING PROTEIN
CN112654704A (zh) 2018-07-30 2021-04-13 丹尼斯科美国公司 包含蛋白质生产率提高表型的突变和遗传修饰的丝状真菌菌株及其方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5246853A (en) 1990-10-05 1993-09-21 Genencor International, Inc. Method for treating cotton-containing fabric with a cellulase composition containing endoglucanase components and which composition is free of exo-cellobiohydrolase I
US5861271A (en) * 1993-12-17 1999-01-19 Fowler; Timothy Cellulase enzymes and systems for their expressions
US7504490B1 (en) * 1998-10-16 2009-03-17 Oscient Pharmaceuticals Corporation Nucleic acid and amino acid sequences relating to Apergillus fumigatus for diagnostics and therapeutics
RU2238974C2 (ru) * 2001-09-06 2004-10-27 ООО НПК "Фермтек" Фрагмент днк мицелиального гриба penicillium verruculosum, кодирующий синтез секретируемой эндоглюканазы iii и группа штаммов penicillium canescens, синтезирующих эндоглюканазу iii penicillium verruculosum, сконструированных методами трансформации и генетической инженерии на основе этого фрагмента днк
CN1688198A (zh) * 2002-09-10 2005-10-26 金克克国际有限公司 使用高浓度糖混合物诱导基因表达
JP4834554B2 (ja) * 2003-11-06 2011-12-14 ジェネンコー・インターナショナル・インク 糸状菌におけるプロテアーゼ抑制剤及びその変異体の発現
US7413887B2 (en) * 2004-05-27 2008-08-19 Genecor International, Inc. Trichoderma reesei glucoamylase and homologs thereof
BRPI0713090A2 (pt) * 2006-06-22 2012-10-30 Iogen Energy Corp composições de enzima e métodos para a hidrólise enzimática aperfeiçoada de celulose
CN101680019A (zh) * 2007-06-15 2010-03-24 丹尼斯科美国公司 有用的真菌菌株的选择
BRPI0816389B1 (pt) 2007-09-07 2020-11-17 Danisco Us Inc. métodos de diminuição da quantidade de uma preparação de celulase total requerida para hidrolisar um material celulósico e de hidrólise de um material celulósico
DK2480660T5 (da) 2009-09-23 2020-11-09 Danisco Us Inc Hidtil ukendte glycosylhydrolaseenzymer og anvendelser heraf

Also Published As

Publication number Publication date
US10266832B2 (en) 2019-04-23
WO2012027580A1 (en) 2012-03-01
CA2808777C (en) 2019-10-29
AU2011293263B2 (en) 2014-10-23
BR112013004419A2 (pt) 2016-05-31
MY160960A (en) 2017-03-31
MX2013002087A (es) 2013-05-09
RU2013112888A (ru) 2014-09-27
CN103097536B (zh) 2016-01-13
KR20130100108A (ko) 2013-09-09
EP2609200B1 (en) 2017-04-26
DK2609200T3 (en) 2017-08-14
CN103097536A (zh) 2013-05-08
US20130224864A1 (en) 2013-08-29
CA2808777A1 (en) 2012-03-01
ZA201208720B (en) 2014-01-29
AU2011293263A1 (en) 2012-12-06
EP2609200A1 (en) 2013-07-03
JP5707494B2 (ja) 2015-04-30
RU2608639C2 (ru) 2017-01-23
KR102004003B1 (ko) 2019-07-25
JP2013533751A (ja) 2013-08-29

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