MX2021016027A - Ensayos y metodos para deteccion de acidos nucleicos. - Google Patents

Ensayos y metodos para deteccion de acidos nucleicos.

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Publication number
MX2021016027A
MX2021016027A MX2021016027A MX2021016027A MX2021016027A MX 2021016027 A MX2021016027 A MX 2021016027A MX 2021016027 A MX2021016027 A MX 2021016027A MX 2021016027 A MX2021016027 A MX 2021016027A MX 2021016027 A MX2021016027 A MX 2021016027A
Authority
MX
Mexico
Prior art keywords
detection
methods
nucleic acids
tests
devices
Prior art date
Application number
MX2021016027A
Other languages
English (en)
Spanish (es)
Inventor
Janice Sha Chen
James Paul Broughton
Jasmeet Singh
Clare Louise Fasching
Maria- Nefeli TSALOGLOU
Xin Miao
Lucas Harrington
Daniel Thomas Drzal
Sarah Jane Shapiro
Pedro Patrick Draper Galarza
Original Assignee
Mammoth Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mammoth Biosciences Inc filed Critical Mammoth Biosciences Inc
Publication of MX2021016027A publication Critical patent/MX2021016027A/es

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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0605—Metering of fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2300/06—Auxiliary integrated devices, integrated components
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    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L2400/0622—Valves, specific forms thereof distribution valves, valves having multiple inlets and/or outlets, e.g. metering valves, multi-way valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L2400/06—Valves, specific forms thereof
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    • B01L2400/0644—Valves, specific forms thereof with moving parts rotary valves
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    • B01L2400/06—Valves, specific forms thereof
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    • B01L2400/0655—Valves, specific forms thereof with moving parts pinch valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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    • B01L2400/0666—Solenoid valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Organic Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)
MX2021016027A 2019-06-18 2020-06-17 Ensayos y metodos para deteccion de acidos nucleicos. MX2021016027A (es)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201962863178P 2019-06-18 2019-06-18
US201962879325P 2019-07-26 2019-07-26
US201962881809P 2019-08-01 2019-08-01
US201962944926P 2019-12-06 2019-12-06
US202062985850P 2020-03-05 2020-03-05
PCT/US2020/038242 WO2020257356A2 (en) 2019-06-18 2020-06-17 Assays and methods for detection of nucleic acids

Publications (1)

Publication Number Publication Date
MX2021016027A true MX2021016027A (es) 2022-04-07

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ID=71527941

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MX2021016027A MX2021016027A (es) 2019-06-18 2020-06-17 Ensayos y metodos para deteccion de acidos nucleicos.

Country Status (11)

Country Link
US (1) US20220325363A1 (https=)
EP (1) EP3986614A2 (https=)
JP (1) JP2022538046A (https=)
KR (1) KR20220035376A (https=)
CN (1) CN114650883B (https=)
AU (1) AU2020296016A1 (https=)
BR (1) BR112021025669A2 (https=)
CA (1) CA3143685A1 (https=)
IL (1) IL289050A (https=)
MX (1) MX2021016027A (https=)
WO (1) WO2020257356A2 (https=)

Families Citing this family (74)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3830301B1 (en) 2018-08-01 2024-05-22 Mammoth Biosciences, Inc. Programmable nuclease compositions and methods of use thereof
AU2019337088A1 (en) 2018-09-03 2021-05-06 Visby Medical, Inc. Devices and methods for antibiotic susceptibility testing
WO2020117715A1 (en) 2018-12-03 2020-06-11 Board Of Regents, The University Of Texas System Oligo-benzamide analogs and their use in cancer treatment
EP3931313A2 (en) 2019-01-04 2022-01-05 Mammoth Biosciences, Inc. Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection
EP3996738A4 (en) * 2019-07-11 2023-07-26 Arbor Biotechnologies, Inc. NOVEL ENZYMES AND SYSTEMS TARGETING CRISPR DNA
CN111024798B (zh) * 2019-12-12 2023-04-28 天津科技大学 一种自动在线监测环境二甲基硫的系统和方法
US11352675B2 (en) 2020-01-03 2022-06-07 Visby Medical, Inc. Devices and methods for antibiotic susceptability testing
US20240279718A1 (en) * 2020-03-18 2024-08-22 University Of Connecticut Crispr-cas12a reaction for rapid and highly sensitive isothermal nucleic acid detection
US11453907B2 (en) 2020-03-23 2022-09-27 The Broad Institute, Inc. Crispr effector system based coronavirus diagnostics
US12442726B2 (en) 2020-04-19 2025-10-14 John J. Daniels Mask-based testing system for detecting biomarkers in exhaled breath condensate, aerosols and gases
US12031982B2 (en) 2020-04-19 2024-07-09 John J. Daniels Using exhaled breath condensate for testing for a biomarker of COVID-19
US12533047B2 (en) 2020-04-19 2026-01-27 John J. Daniels Mask-based diagnostic system using exhaled breath condensate
US12369816B2 (en) 2020-04-19 2025-07-29 John J. Daniels Mask-based diagnostic system using exhaled breath condensate
CA3078004A1 (en) * 2020-04-23 2021-10-23 Oz Optics Ltd. Covid-19 detection pipeline
EP4143348A4 (en) * 2020-04-29 2024-01-17 Lu, Shi-Long VIRAL TEST IN SALIVA
CA3178629A1 (en) * 2020-05-29 2021-12-02 Jesus Ching Programmable nuclease diagnostic device
CN114381360A (zh) * 2020-10-22 2022-04-22 佳能医疗系统株式会社 核酸检测系统及其应用
US20220127685A1 (en) * 2020-10-22 2022-04-28 Canon Medical Systems Corporation Nucleic acid detection system and nucleic acid detection method
US20220136074A1 (en) * 2020-11-05 2022-05-05 The Governors Of The University Of Alberta Isothermal amplification and ambient visualization in a single tube for the detection of sars-cov-2 using loop-mediated amplification and crispr technology
US12492398B2 (en) * 2020-11-23 2025-12-09 Zunyi Yang Compositions for the multiplexed detection of viruses
JP2024502387A (ja) * 2021-01-05 2024-01-18 マーチ・セラピューティクス，インコーポレーテッド 核酸を検出するための方法
TW202246524A (zh) * 2021-01-15 2022-12-01 普渡研究基金會 於固相介質上的環介導恆溫擴增(lamp)
US20220235408A1 (en) 2021-01-15 2022-07-28 Purdue Research Foundation Loop-mediated isothermal amplification (lamp) on a solid-phase medium
TW202244279A (zh) * 2021-01-15 2022-11-16 普渡研究基金會 於固相介質上的環介導恆溫擴增(lamp)
WO2022159822A1 (en) * 2021-01-25 2022-07-28 The Regents Of The University Of California Crispr-cas effector polypeptides and methods of use thereof
WO2022170013A1 (en) * 2021-02-05 2022-08-11 University Of Connecticut Multiplexed, crispr-based diagnostics of sars-cov-2 in autonomous microfluidic device
DE102021104908B3 (de) * 2021-03-01 2022-06-15 BionLYX GmbH Vorrichtung, System und Verfahren zur quantitativen Real-Time PCR-Analyse (qPCR)
WO2022189784A1 (en) * 2021-03-10 2022-09-15 Phoenix Dx Ltd Nucleic acid amplification, kits, methods, and uses
WO2022192108A1 (en) * 2021-03-12 2022-09-15 The Regents Of The University Of Colorado, A Body Corporate Methods and devices for nucleic acid detection
US12590340B2 (en) * 2021-05-10 2026-03-31 The Royal Institution For The Advancement Of Learning/Mcgill University Microfluidic plasmonic color reading chips and methods
WO2022253960A2 (en) * 2021-06-02 2022-12-08 University Of Copenhagen Mutant cas12j endonucleases
US20240287592A1 (en) * 2021-06-10 2024-08-29 New England Biolabs, Inc. An Isothermal Diagnostic Test that Utilizes a Cas Protein and a Polymerase
EP4355909A4 (en) * 2021-06-17 2025-06-11 Mammoth Biosciences, Inc. DEVICES, SYSTEMS AND METHODS FOR THE ANALYSIS OF NUCLEIC ACIDS
US20230002805A1 (en) * 2021-06-25 2023-01-05 Enzo Biochem, Inc. Use of organic cationic compounds to accelerate nucleic acid hybridization, synthesis, and amplification
WO2023279042A2 (en) * 2021-07-02 2023-01-05 Siemens Healthcare Laboratory, Llc Compositions and methods for detection of severe acute respiratory syndrome coronavirus 2 variants
WO2023283622A1 (en) 2021-07-08 2023-01-12 Montana State University Crispr-based programmable rna editing
WO2023003534A1 (en) * 2021-07-19 2023-01-26 Hewlett-Packard Development Company, L.P. Nucleic acid testing devices including an actuating reagent chamber
WO2023004391A2 (en) 2021-07-21 2023-01-26 Montana State University Nucleic acid detection using type iii crispr complex
WO2023015259A2 (en) * 2021-08-05 2023-02-09 Mammoth Biosciences, Inc. Methods and compositions for improved snp discrimination
US20250128262A1 (en) * 2021-08-13 2025-04-24 Visby Medical, Inc. Molecular diagnostic devices and methods for retaining and mixing reagents
WO2023023678A2 (en) * 2021-08-16 2023-02-23 Diametrics, Inc. Diagnostic platform for testing exhaled breath condensate and universal biosensor
CN113667718B (zh) * 2021-08-25 2023-11-28 山东舜丰生物科技有限公司 利用双链核酸检测器进行靶核酸检测的方法
CN113621737A (zh) * 2021-09-06 2021-11-09 辽宁佰昊生物科技有限公司 一种流感病毒检测试剂盒及其应用
WO2023039491A2 (en) * 2021-09-09 2023-03-16 Proof Diagnostics, Inc. Coronavirus rapid diagnostics
EP4405103A4 (en) * 2021-09-24 2025-08-20 Cubit Diagnostics Inc TIMED SYSTEM FOR FAST DOSING
WO2023056451A1 (en) * 2021-09-30 2023-04-06 Mammoth Biosciences, Inc. Compositions and methods for assaying for and genotyping genetic variations
US20250304934A1 (en) * 2021-12-14 2025-10-02 The Broad Institute, Inc. Reprogrammable fanzor polynucleotides and uses thereof
WO2023122648A1 (en) * 2021-12-23 2023-06-29 Mammoth Biosciences, Inc. Devices, systems, and methods for detecting target nucleic acids
US20230226543A1 (en) * 2022-01-20 2023-07-20 National Tsing Hua University Microfluidic device for sars-cov-2 detection and method using the same
CN114317834A (zh) * 2022-02-25 2022-04-12 军事科学院军事医学研究院环境医学与作业医学研究所 一种用于检测新冠病毒的试剂盒及检测方法
CN114836309A (zh) * 2022-04-15 2022-08-02 上海仁度生物科技股份有限公司 全自动核酸检测分析设备及系统
WO2024020373A2 (en) * 2022-07-18 2024-01-25 Mammoth Biosciences, Inc. Devices, systems and methods for analysis of nucleic acids
TWI868818B (zh) * 2022-07-25 2025-01-01 國立中央大學 微流道檢測裝置
US20260071255A1 (en) * 2022-09-07 2026-03-12 Shubhendra Singh Thakur A non-invasive device and method for detecting rna associated disease
WO2024073730A2 (en) * 2022-09-29 2024-04-04 The University Of Chicago Methods and systems for rna sequencing and analysis
CN221028439U (zh) * 2022-09-30 2024-05-28 台达电子国际（新加坡）私人有限公司 可携式核酸检测装置
KR102741082B1 (ko) * 2022-10-26 2024-12-12 가천대학교 산학협력단 유전자가위 기반 쯔쯔가무시증의 신속 진단 방법
WO2024108016A2 (en) * 2022-11-16 2024-05-23 Mammoth Biosciences, Inc. Methods for quantitation of nucleic acid targets
WO2024138016A1 (en) * 2022-12-21 2024-06-27 Mayo Foundation For Medical Education And Research Methods and materials for performing electrochemical assays
US20240226888A1 (en) * 2023-01-11 2024-07-11 Carterra, Inc. Microfluidic flow cell arrays
WO2024159093A2 (en) * 2023-01-26 2024-08-02 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Diagnostic using crispr rna and cas 13 a enzyme
WO2024167317A1 (ko) * 2023-02-10 2024-08-15 주식회사 엔세이지 신규 CRISPR/Cas12a 조성물 및 이의 표적 핵산 검출 용도
CN116287467A (zh) * 2023-03-30 2023-06-23 中国人民解放军军事科学院军事医学研究院 基于CRISPR/Cas的电化学生物传感器及其在核酸检测中的应用
WO2024220581A2 (en) * 2023-04-17 2024-10-24 The General Hospital Corporation Multiplexed crispr genotyping for apol1
CN116660345B (zh) * 2023-04-25 2024-09-06 苏州中科苏净生物技术有限公司 一种基于CRISPR/Cas的电化学核酸检测传感元件及检测方法
WO2024238539A1 (en) * 2023-05-15 2024-11-21 The Regents Of The University Of Michigan Compositions and methods for the analysis of loop-mediated amplification of cell-free dna
CN116590387B (zh) * 2023-07-06 2023-12-08 深圳大学 一种基于CRISPR系统的ssDNA检测方法及应用
WO2025010419A1 (en) * 2023-07-06 2025-01-09 Sekisui Diagnostics, Llc System, device, and method for detecting nucleic acids
KR20250023075A (ko) * 2023-08-09 2025-02-18 경희대학교 산학협력단 유전자 분석용 카트리지 및 이를 포함하는 유전자 분석장치
WO2025049623A1 (en) * 2023-08-29 2025-03-06 Mammoth Biosciences, Inc. Devices, systems and methods for analysis of nucleic acids
FR3152728A1 (fr) 2023-09-12 2025-03-14 Bforcure Bouchon pour un dispositif de diffusion de réactif pour échantillons biologiques
CN117512220A (zh) * 2023-12-06 2024-02-06 湖北省农业科学院经济作物研究所 一种草莓白化相关病毒的检测方法及其应用
WO2025250443A1 (en) * 2024-05-27 2025-12-04 The Regents Of The University Of California Split guide rna compositions and methods for activating a crispr-cas effector protein with a short nucleotide sequence
CN121109659A (zh) * 2025-09-28 2025-12-12 上海市重大传染病和生物安全研究院 一种针对呼吸道病原体rna的多重tma-crispr试剂盒及其应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6824980B2 (en) * 2000-06-08 2004-11-30 Xiao Bing Wang Isometric primer extension method and kit for detection and quantification of specific nucleic acid
AU2003900368A0 (en) * 2003-01-24 2003-02-13 Human Genetic Signatures Pty Ltd Assay for nucleic acid molecules
US7759062B2 (en) * 2006-06-09 2010-07-20 Third Wave Technologies, Inc. T-structure invasive cleavage assays, consistent nucleic acid dispensing, and low level target nucleic acid detection
US20090186344A1 (en) * 2008-01-23 2009-07-23 Caliper Life Sciences, Inc. Devices and methods for detecting and quantitating nucleic acids using size separation of amplicons
CN104040238B (zh) * 2011-11-04 2017-06-27 汉迪拉布公司 多核苷酸样品制备装置
CN103436608B (zh) * 2013-08-08 2015-02-25 中国科学院广州生物医药与健康研究院 基于核酸适体的快速检测方法及试剂盒
WO2015069787A1 (en) * 2013-11-05 2015-05-14 Htg Molecular Diagnostics, Inc. Methods for detecting nucleic acids
ES2898103T3 (es) * 2014-12-31 2022-03-03 Visby Medical Inc Dispositivos para pruebas de diagnóstico molecular
CN110506128A (zh) * 2016-12-09 2019-11-26 博德研究所 基于crispr效应系统的诊断
US10346454B2 (en) * 2017-04-17 2019-07-09 Mammoth Medical, Llc System and method for automated multi-dimensional network management
WO2019079787A1 (en) * 2017-10-20 2019-04-25 The Regents Of The University Of California MICROFLUIDIC SYSTEMS AND METHODS FOR CELL TRANSFECTION VIA LIPOFLEX
EP3830301B1 (en) * 2018-08-01 2024-05-22 Mammoth Biosciences, Inc. Programmable nuclease compositions and methods of use thereof

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