BR112019002679A2 - redução de sinal de ácidos nucleicos contaminantes - Google Patents

redução de sinal de ácidos nucleicos contaminantes

Info

Publication number
BR112019002679A2
BR112019002679A2 BR112019002679-7A BR112019002679A BR112019002679A2 BR 112019002679 A2 BR112019002679 A2 BR 112019002679A2 BR 112019002679 A BR112019002679 A BR 112019002679A BR 112019002679 A2 BR112019002679 A2 BR 112019002679A2
Authority
BR
Brazil
Prior art keywords
nucleic acids
contaminating nucleic
signal reduction
contaminating
reduction
Prior art date
Application number
BR112019002679-7A
Other languages
English (en)
Inventor
Timothy A. BLAUWKAMP
Fred Christians
Michael Rosen
Igor D. VILFAN
Original Assignee
Karius, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Karius, Inc. filed Critical Karius, Inc.
Publication of BR112019002679A2 publication Critical patent/BR112019002679A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/30—Characterised by physical treatment
    • C12Q2523/313—Irradiation, e.g. UV irradiation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

esta invenção proporciona métodos que são úteis para redução ou inativação de ácidos nucleicos contaminantes.
BR112019002679-7A 2016-08-08 2017-08-07 redução de sinal de ácidos nucleicos contaminantes BR112019002679A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662372269P 2016-08-08 2016-08-08
US62/372,269 2016-08-08
PCT/US2017/045782 WO2018031486A1 (en) 2016-08-08 2017-08-07 Reduction of signal from contaminant nucleic acids

Publications (1)

Publication Number Publication Date
BR112019002679A2 true BR112019002679A2 (pt) 2019-05-14

Family

ID=61163308

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112019002679-7A BR112019002679A2 (pt) 2016-08-08 2017-08-07 redução de sinal de ácidos nucleicos contaminantes

Country Status (5)

Country Link
US (1) US20190256891A1 (pt)
EP (2) EP3497218B1 (pt)
BR (1) BR112019002679A2 (pt)
CA (1) CA3031231A1 (pt)
WO (1) WO2018031486A1 (pt)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9976181B2 (en) 2016-03-25 2018-05-22 Karius, Inc. Synthetic nucleic acid spike-ins
US10697008B2 (en) 2017-04-12 2020-06-30 Karius, Inc. Sample preparation methods, systems and compositions
US11519024B2 (en) 2017-08-04 2022-12-06 Billiontoone, Inc. Homologous genomic regions for characterization associated with biological targets
CA3071855C (en) 2017-08-04 2021-09-14 Billiontoone, Inc. Target-associated molecules for characterization associated with biological targets
KR102372572B1 (ko) 2017-08-04 2022-03-08 빌리언투원, 인크. 생물학적 표적과 연관된 정량화에서 표적 연관 분자를 이용한 서열분석 출력값 측정 및 분석
DK3735470T3 (da) 2018-01-05 2024-02-26 Billiontoone Inc Kvalitetskontroltemplates til sikring af validiteten af sekventeringsbaserede analyser
WO2019178157A1 (en) * 2018-03-16 2019-09-19 Karius, Inc. Sample series to differentiate target nucleic acids from contaminant nucleic acids
CA3107221C (en) * 2019-08-23 2022-02-22 Mcmaster University Suppressing false positives (type i error) during analysis of sample biological materials

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US6071745A (en) * 1997-06-27 2000-06-06 Bio-Rad Laboratories Method and formulation for lyophilizing cultured human cells to preserve RNA and DNA contained in cells for use in molecular biology experiments
US6696019B2 (en) * 1998-06-15 2004-02-24 Bbi Bioseq, Inc. Rapid cryobaric sterilization and vaccine preparation
US8361712B2 (en) * 2007-12-17 2013-01-29 General Electric Company Contamination-free reagents for nucleic acid amplification
WO2003087402A1 (en) * 2002-04-11 2003-10-23 Infectio Diagnostic (I.D.I.) Inc. Method for the preparation of reagents for amplification and/or detection of nucleic acids that exhibit no significant contamination by nucleic acids
JP2006505275A (ja) * 2002-11-12 2006-02-16 ジェノライフ 工業製品において微生物を普遍的に検出するための一段階リアルタイムｒｔ−ｐｃｒキット
US20060246457A1 (en) * 2003-07-02 2006-11-02 Keygene N.V. Splice site aflp
BRPI0515418A (pt) * 2004-08-16 2008-07-22 Nature Technology Corp método para a produção de dna de plasmìdio super-enrolado fechado covalentemente e composição de matéria
US20090233291A1 (en) * 2005-06-06 2009-09-17 454 Life Sciences Corporation Paired end sequencing
AR066922A1 (es) * 2007-06-08 2009-09-23 Monsanto Technology Llc Metodos de mejoramiento molecular del germoplasma de una planta por secuenciamiento dirigido
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GB2474225A (en) * 2009-07-21 2011-04-13 Biotec Pharmacon Asa DNase for decontamination of reverse transcription and amplification reactions
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Also Published As

Publication number Publication date
EP3497218A4 (en) 2020-03-18
EP3497218A1 (en) 2019-06-19
EP4074824A1 (en) 2022-10-19
WO2018031486A1 (en) 2018-02-15
EP3497218B1 (en) 2022-03-16
CA3031231A1 (en) 2018-02-15
US20190256891A1 (en) 2019-08-22

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Legal Events

Date Code Title Description
B350 Update of information on the portal [chapter 15.35 patent gazette]
B06W Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements