DK3405580T3 - Ekspressionssystem til eukaryotiske organismer - Google Patents

Ekspressionssystem til eukaryotiske organismer Download PDF

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Publication number
DK3405580T3
DK3405580T3 DK17712530.9T DK17712530T DK3405580T3 DK 3405580 T3 DK3405580 T3 DK 3405580T3 DK 17712530 T DK17712530 T DK 17712530T DK 3405580 T3 DK3405580 T3 DK 3405580T3
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DK
Denmark
Prior art keywords
expression system
eukaryotic organisms
eukaryotic
organisms
expression
Prior art date
Application number
DK17712530.9T
Other languages
English (en)
Inventor
Dominik Mojzita
Anssi Rantasalo
Jussi Jäntti
Christopher Landowski
Joosu Kuivanen
Original Assignee
Teknologian Tutkimuskeskus Vtt Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Teknologian Tutkimuskeskus Vtt Oy filed Critical Teknologian Tutkimuskeskus Vtt Oy
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Publication of DK3405580T3 publication Critical patent/DK3405580T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • C12N15/625DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/12Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/122Hairpin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/50Physical structure
    • C12N2310/53Physical structure partially self-complementary or closed
    • C12N2310/531Stem-loop; Hairpin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK17712530.9T 2016-02-22 2017-02-21 Ekspressionssystem til eukaryotiske organismer DK3405580T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI20165137A FI127283B (en) 2016-02-22 2016-02-22 Expression system for eukaryotic microorganisms
PCT/FI2017/050114 WO2017144777A1 (en) 2016-02-22 2017-02-21 Expression system for eukaryotic organisms

Publications (1)

Publication Number Publication Date
DK3405580T3 true DK3405580T3 (da) 2021-05-25

Family

ID=58387847

Family Applications (1)

Application Number Title Priority Date Filing Date
DK17712530.9T DK3405580T3 (da) 2016-02-22 2017-02-21 Ekspressionssystem til eukaryotiske organismer

Country Status (16)

Country Link
US (1) US11332750B2 (da)
EP (1) EP3405580B1 (da)
JP (1) JP6953667B2 (da)
KR (1) KR20180117147A (da)
CN (1) CN109477115B (da)
AU (1) AU2017224865B2 (da)
BR (1) BR112018017088A2 (da)
CA (1) CA3015440A1 (da)
DK (1) DK3405580T3 (da)
ES (1) ES2870962T3 (da)
FI (1) FI127283B (da)
HU (1) HUE054884T2 (da)
LT (1) LT3405580T (da)
MX (1) MX2018010055A (da)
PT (1) PT3405580T (da)
WO (1) WO2017144777A1 (da)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI129102B (en) * 2018-03-19 2021-07-15 Teknologian Tutkimuskeskus Vtt Oy Heterological production of psilosybin
GB201903244D0 (en) * 2019-03-14 2019-04-24 Syndermix Ag Methods for lectin production with improved yield
BR112022001407A2 (pt) * 2019-08-01 2022-05-03 Memorial Hospital For Cancer And Allied Diseases Células para imunoterapia melhorada e uso das mesmas
CA3161146A1 (en) * 2019-11-19 2021-05-27 Teknologian Tutkimuskeskus Vtt Oy Non-viral transcription activation domains and methods and uses related thereto
WO2021220209A1 (en) 2020-04-30 2021-11-04 Dyadic International (Usa), Inc. Nicotinamide riboside production in filamentous fungi
MX2023002681A (es) * 2020-09-05 2023-04-03 Ginkgo Bioworks Inc Sistemas de expresion sintetica.
FR3122436A1 (fr) 2021-04-30 2022-11-04 IFP Energies Nouvelles Insertion multicopies d’un gène d’intérêt dans le génome d’un champignon
WO2022251056A1 (en) 2021-05-27 2022-12-01 Novozymes A/S Transcriptional regulators and polynucleotides encoding the same
WO2023094429A1 (en) * 2021-11-23 2023-06-01 Kaesler Nutrition Gmbh Artificial yeast promoter regions
CN113846024B (zh) * 2021-12-01 2022-03-29 南京昊禾生物科技有限公司 一种降低l-苹果酸发酵过程中副产物富马酸的方法、菌种及应用
US20230372467A1 (en) 2022-05-20 2023-11-23 Phibro Animal Health Corporation Using fungal constructs to produce viral protein antigens
WO2023242273A1 (en) 2022-06-14 2023-12-21 Newmilkbuzz B.V. Expression of milk caseins in filamentous fungi

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020081667A1 (en) 2000-12-06 2002-06-27 Jorn Gorlach Self-amplifying transcriptional amplification system

Also Published As

Publication number Publication date
WO2017144777A1 (en) 2017-08-31
CN109477115B (zh) 2022-06-03
JP6953667B2 (ja) 2021-10-27
US20180371468A1 (en) 2018-12-27
EP3405580A1 (en) 2018-11-28
JP2019505230A (ja) 2019-02-28
AU2017224865B2 (en) 2022-07-07
CN109477115A (zh) 2019-03-15
FI20165137A (fi) 2017-08-23
PT3405580T (pt) 2021-05-21
US11332750B2 (en) 2022-05-17
AU2017224865A1 (en) 2018-09-13
FI127283B (en) 2018-03-15
KR20180117147A (ko) 2018-10-26
MX2018010055A (es) 2018-11-09
EP3405580B1 (en) 2021-03-31
BR112018017088A2 (pt) 2019-01-02
ES2870962T3 (es) 2021-10-28
CA3015440A1 (en) 2017-08-31
LT3405580T (lt) 2021-08-10
HUE054884T2 (hu) 2021-10-28

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