FI20165137A - Ekspressiojärjestelmä eukaryoottisille mikro-organismeille - Google Patents

Ekspressiojärjestelmä eukaryoottisille mikro-organismeille

Info

Publication number
FI20165137A
FI20165137A FI20165137A FI20165137A FI20165137A FI 20165137 A FI20165137 A FI 20165137A FI 20165137 A FI20165137 A FI 20165137A FI 20165137 A FI20165137 A FI 20165137A FI 20165137 A FI20165137 A FI 20165137A
Authority
FI
Finland
Prior art keywords
expression system
eukaryotic microorganisms
eukaryotic
microorganisms
expression
Prior art date
Application number
FI20165137A
Other languages
English (en)
Swedish (sv)
Other versions
FI127283B (fi
Inventor
Dominik Mojzita
Anssi Rantasalo
Jussi Jäntti
Christopher Landowski
Joosu Kuivanen
Original Assignee
Teknologian Tutkimuskeskus Vtt Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to FI20165137A priority Critical patent/FI127283B/fi
Application filed by Teknologian Tutkimuskeskus Vtt Oy filed Critical Teknologian Tutkimuskeskus Vtt Oy
Priority to US15/737,696 priority patent/US11332750B2/en
Priority to BR112018017088A priority patent/BR112018017088A2/pt
Priority to MX2018010055A priority patent/MX2018010055A/es
Priority to JP2018544246A priority patent/JP6953667B2/ja
Priority to PT177125309T priority patent/PT3405580T/pt
Priority to EP17712530.9A priority patent/EP3405580B1/en
Priority to CA3015440A priority patent/CA3015440A1/en
Priority to HUE17712530A priority patent/HUE054884T2/hu
Priority to ES17712530T priority patent/ES2870962T3/es
Priority to CN201780020813.9A priority patent/CN109477115B/zh
Priority to KR1020187027693A priority patent/KR20180117147A/ko
Priority to DK17712530.9T priority patent/DK3405580T3/da
Priority to PCT/FI2017/050114 priority patent/WO2017144777A1/en
Priority to AU2017224865A priority patent/AU2017224865B2/en
Priority to LTEP17712530.9T priority patent/LT3405580T/lt
Publication of FI20165137A publication Critical patent/FI20165137A/fi
Application granted granted Critical
Publication of FI127283B publication Critical patent/FI127283B/fi

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • C12N15/625DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/12Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/122Hairpin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/50Physical structure
    • C12N2310/53Physical structure partially self-complementary or closed
    • C12N2310/531Stem-loop; Hairpin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
FI20165137A 2016-02-22 2016-02-22 Ekspressiojärjestelmä eukaryoottisille mikro-organismeille FI127283B (fi)

Priority Applications (16)

Application Number Priority Date Filing Date Title
FI20165137A FI127283B (fi) 2016-02-22 2016-02-22 Ekspressiojärjestelmä eukaryoottisille mikro-organismeille
CN201780020813.9A CN109477115B (zh) 2016-02-22 2017-02-21 用于真核生物的表达系统
MX2018010055A MX2018010055A (es) 2016-02-22 2017-02-21 Sistema de expresion para organismos eucarioticos.
JP2018544246A JP6953667B2 (ja) 2016-02-22 2017-02-21 真核生物のための発現系
PT177125309T PT3405580T (pt) 2016-02-22 2017-02-21 Sistema de expressão para organismos eucarióticos
EP17712530.9A EP3405580B1 (en) 2016-02-22 2017-02-21 Expression system for eukaryotic organisms
CA3015440A CA3015440A1 (en) 2016-02-22 2017-02-21 Expression system for eukaryotic organisms
HUE17712530A HUE054884T2 (hu) 2016-02-22 2017-02-21 Expressziós rendszer eukarióta szervezetekhez
US15/737,696 US11332750B2 (en) 2016-02-22 2017-02-21 Expression system for eukaryotic organisms
BR112018017088A BR112018017088A2 (pt) 2016-02-22 2017-02-21 sistema de expressão para organismos eucarióticos
KR1020187027693A KR20180117147A (ko) 2016-02-22 2017-02-21 진핵생물을 위한 발현 시스템
DK17712530.9T DK3405580T3 (da) 2016-02-22 2017-02-21 Ekspressionssystem til eukaryotiske organismer
PCT/FI2017/050114 WO2017144777A1 (en) 2016-02-22 2017-02-21 Expression system for eukaryotic organisms
AU2017224865A AU2017224865B2 (en) 2016-02-22 2017-02-21 Expression system for eukaryotic organisms
LTEP17712530.9T LT3405580T (lt) 2016-02-22 2017-02-21 Eukariotinių organizmų ekspresijos sistema
ES17712530T ES2870962T3 (es) 2016-02-22 2017-02-21 Sistema de expresión para organismos eucarióticos

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FI20165137A FI127283B (fi) 2016-02-22 2016-02-22 Ekspressiojärjestelmä eukaryoottisille mikro-organismeille

Publications (2)

Publication Number Publication Date
FI20165137A true FI20165137A (fi) 2017-08-23
FI127283B FI127283B (fi) 2018-03-15

Family

ID=58387847

Family Applications (1)

Application Number Title Priority Date Filing Date
FI20165137A FI127283B (fi) 2016-02-22 2016-02-22 Ekspressiojärjestelmä eukaryoottisille mikro-organismeille

Country Status (16)

Country Link
US (1) US11332750B2 (fi)
EP (1) EP3405580B1 (fi)
JP (1) JP6953667B2 (fi)
KR (1) KR20180117147A (fi)
CN (1) CN109477115B (fi)
AU (1) AU2017224865B2 (fi)
BR (1) BR112018017088A2 (fi)
CA (1) CA3015440A1 (fi)
DK (1) DK3405580T3 (fi)
ES (1) ES2870962T3 (fi)
FI (1) FI127283B (fi)
HU (1) HUE054884T2 (fi)
LT (1) LT3405580T (fi)
MX (1) MX2018010055A (fi)
PT (1) PT3405580T (fi)
WO (1) WO2017144777A1 (fi)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI129102B (fi) * 2018-03-19 2021-07-15 Teknologian Tutkimuskeskus Vtt Oy Psilosybiinin heterologinen tuotanto
GB201903244D0 (en) 2019-03-14 2019-04-24 Syndermix Ag Methods for lectin production with improved yield
CN114450397A (zh) * 2019-08-01 2022-05-06 纪念斯隆-凯特琳癌症中心 用于改善免疫疗法的细胞及其用途
CA3161146A1 (en) * 2019-11-19 2021-05-27 Teknologian Tutkimuskeskus Vtt Oy Non-viral transcription activation domains and methods and uses related thereto
WO2021220209A1 (en) 2020-04-30 2021-11-04 Dyadic International (Usa), Inc. Nicotinamide riboside production in filamentous fungi
MX2023002681A (es) * 2020-09-05 2023-04-03 Ginkgo Bioworks Inc Sistemas de expresion sintetica.
FR3122436A1 (fr) 2021-04-30 2022-11-04 IFP Energies Nouvelles Insertion multicopies d’un gène d’intérêt dans le génome d’un champignon
WO2022251056A1 (en) 2021-05-27 2022-12-01 Novozymes A/S Transcriptional regulators and polynucleotides encoding the same
WO2023094429A1 (en) * 2021-11-23 2023-06-01 Kaesler Nutrition Gmbh Artificial yeast promoter regions
CN113846024B (zh) * 2021-12-01 2022-03-29 南京昊禾生物科技有限公司 一种降低l-苹果酸发酵过程中副产物富马酸的方法、菌种及应用
US20230372467A1 (en) 2022-05-20 2023-11-23 Phibro Animal Health Corporation Using fungal constructs to produce viral protein antigens
WO2023242273A1 (en) 2022-06-14 2023-12-21 Newmilkbuzz B.V. Expression of milk caseins in filamentous fungi

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020081667A1 (en) * 2000-12-06 2002-06-27 Jorn Gorlach Self-amplifying transcriptional amplification system

Also Published As

Publication number Publication date
CN109477115A (zh) 2019-03-15
FI127283B (fi) 2018-03-15
BR112018017088A2 (pt) 2019-01-02
AU2017224865A1 (en) 2018-09-13
JP2019505230A (ja) 2019-02-28
PT3405580T (pt) 2021-05-21
CN109477115B (zh) 2022-06-03
JP6953667B2 (ja) 2021-10-27
WO2017144777A1 (en) 2017-08-31
EP3405580B1 (en) 2021-03-31
HUE054884T2 (hu) 2021-10-28
AU2017224865B2 (en) 2022-07-07
MX2018010055A (es) 2018-11-09
US11332750B2 (en) 2022-05-17
DK3405580T3 (da) 2021-05-25
ES2870962T3 (es) 2021-10-28
KR20180117147A (ko) 2018-10-26
LT3405580T (lt) 2021-08-10
CA3015440A1 (en) 2017-08-31
US20180371468A1 (en) 2018-12-27
EP3405580A1 (en) 2018-11-28

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