DK3167065T3 - Dobbelt cistronisk bakterielt ekspressionssystem - Google Patents

Dobbelt cistronisk bakterielt ekspressionssystem Download PDF

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Publication number
DK3167065T3
DK3167065T3 DK15745247.5T DK15745247T DK3167065T3 DK 3167065 T3 DK3167065 T3 DK 3167065T3 DK 15745247 T DK15745247 T DK 15745247T DK 3167065 T3 DK3167065 T3 DK 3167065T3
Authority
DK
Denmark
Prior art keywords
expression system
bacterial expression
cistronic
double
double cistronic
Prior art date
Application number
DK15745247.5T
Other languages
English (en)
Inventor
Shardul Salunkhe
Brajesh Varshney
Sudheerbabu Soorapaneni
Original Assignee
Lupin Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lupin Ltd filed Critical Lupin Ltd
Application granted granted Critical
Publication of DK3167065T3 publication Critical patent/DK3167065T3/da

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/34—Cucurbitaceae, e.g. bitter melon, cucumber or watermelon 
    • A01H6/342—Citrullus lanatus [watermelon]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/27—Erwinia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/605—Glucagons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24029—Aureolysin (3.4.24.29)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/51—Complete heavy chain or Fd fragment, i.e. VH + CH1
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/515—Complete light chain, i.e. VL + CL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Botany (AREA)
  • Physiology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DK15745247.5T 2014-07-09 2015-07-09 Dobbelt cistronisk bakterielt ekspressionssystem DK3167065T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN2245MU2014 2014-07-09
PCT/IB2015/055189 WO2016005931A1 (en) 2014-07-09 2015-07-09 Dual cistronic bacterial expression system

Publications (1)

Publication Number Publication Date
DK3167065T3 true DK3167065T3 (da) 2020-07-06

Family

ID=53773474

Family Applications (1)

Application Number Title Priority Date Filing Date
DK15745247.5T DK3167065T3 (da) 2014-07-09 2015-07-09 Dobbelt cistronisk bakterielt ekspressionssystem

Country Status (20)

Country Link
US (2) US10329571B2 (da)
EP (1) EP3167065B1 (da)
JP (1) JP6748061B2 (da)
CN (1) CN106661588B (da)
AU (1) AU2015287296A1 (da)
BR (1) BR112017000389A2 (da)
CA (1) CA2954594A1 (da)
CY (1) CY1123303T1 (da)
DK (1) DK3167065T3 (da)
ES (1) ES2808906T3 (da)
HR (1) HRP20200996T1 (da)
HU (1) HUE050300T2 (da)
LT (1) LT3167065T (da)
MX (1) MX2017000332A (da)
PH (1) PH12017500047A1 (da)
PL (1) PL3167065T3 (da)
PT (1) PT3167065T (da)
RU (1) RU2732148C2 (da)
SI (1) SI3167065T1 (da)
WO (1) WO2016005931A1 (da)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6435770B2 (ja) * 2014-01-21 2018-12-12 東ソー株式会社 シグナルペプチドをコードするポリヌクレオチドおよびそれを用いたタンパク質の製造方法
WO2017021819A1 (en) * 2015-07-31 2017-02-09 Dr. Reddy’S Laboratories Limited Process for preparation of protein or peptide
EP3624847A1 (en) * 2017-05-19 2020-03-25 Council of Scientific and Industrial Research A method for producing refolded recombinant humanized ranibizumab
WO2019038338A1 (en) 2017-08-23 2019-02-28 Sandoz Ag PROTEIN EXPRESSION SYSTEM WITH MULTIPLE GENIC COPIES
JP7185006B2 (ja) * 2018-07-06 2022-12-06 ワッカー ケミー アクチエンゲゼルシャフト 発酵法で組換えタンパク質を放出する細菌株
JP2020120622A (ja) * 2019-01-31 2020-08-13 Ｓｐｉｂｅｒ株式会社 組換え細胞、発現構築物、及び組換えタンパク質の製造方法
US20220177562A1 (en) * 2019-04-02 2022-06-09 Council Of Scientific & Industrial Research Cloning and expression of in vivo refolded antibody fragment
CN114774453B (zh) * 2022-03-14 2023-04-21 湖北大学 一种运动发酵单胞菌基因表达严谨调控系统的构建方法和应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7018809B1 (en) 1991-09-19 2006-03-28 Genentech, Inc. Expression of functional antibody fragments
CA2430182C (en) * 2000-12-14 2011-01-25 Genentech, Inc. Prokaryotically produced antibodies and uses thereof
CA2454731C (en) 2001-08-27 2010-11-02 Genentech, Inc. A system for antibody expression and assembly
HUE032654T2 (en) * 2006-07-10 2017-10-30 Esbatech Alcon Biomed Res Unit scFv antibodies that cross the epithelial and / or endothelial layers
RU2413769C1 (ru) * 2009-12-24 2011-03-10 Учреждение Российской академии наук Институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова РАН РЕКОМБИНАНТНАЯ ПЛАЗМИДНАЯ ДНК pET-32a, КОДИРУЮЩАЯ ГЕН ЛИГАНД-СВЯЗЫВАЮЩЕГО ДОМЕНА РЕЦЕПТОРА II ТИПА ТРАНСФОРМИРУЮЩЕГО ФАКТОРА РОСТА-β ЧЕЛОВЕКА (TβRII), ШТАММ БАКТЕРИЙ Escherichia coli - ПРОДУЦЕНТ СЛИТНОГО БЕЛКА ТИОРЕДОКСИН/TβRII И СПОСОБ РЕНАТУРАЦИИ И ОЧИСТКИ ЦЕЛЕВОГО БЕЛКА TβRII
GB201002730D0 (en) * 2010-02-18 2010-04-07 Uni I Oslo Product
US8853380B2 (en) 2010-06-04 2014-10-07 Lupin Limited Modified SAK gene for the production of recombinant proteins
PL2964768T3 (pl) * 2013-03-08 2021-01-11 Neuclone Biologics Pty Ltd System ekspresji komórkowej
MX368142B (es) * 2013-04-30 2019-09-20 Intas Pharmaceuticals Ltd Metodo de clonacion, expresion y purificacion novedoso para la preparacion de ranibizumab.
TW201534726A (zh) * 2013-07-03 2015-09-16 Halozyme Inc 熱穩定ｐｈ２０玻尿酸酶變異體及其用途

Also Published As

Publication number Publication date
PL3167065T3 (pl) 2020-11-16
BR112017000389A2 (pt) 2018-01-23
EP3167065A1 (en) 2017-05-17
LT3167065T (lt) 2020-07-27
US20190309313A1 (en) 2019-10-10
MX2017000332A (es) 2017-07-28
HRP20200996T1 (hr) 2021-01-08
ES2808906T3 (es) 2021-03-02
JP2017520261A (ja) 2017-07-27
US11365418B2 (en) 2022-06-21
AU2015287296A1 (en) 2017-02-02
US10329571B2 (en) 2019-06-25
PH12017500047A1 (en) 2017-05-22
SI3167065T1 (sl) 2020-09-30
EP3167065B1 (en) 2020-03-25
HUE050300T2 (hu) 2020-11-30
CY1123303T1 (el) 2021-12-31
RU2732148C2 (ru) 2020-09-11
CA2954594A1 (en) 2016-01-14
US20170159060A1 (en) 2017-06-08
CN106661588B (zh) 2020-11-06
CN106661588A (zh) 2017-05-10
PT3167065T (pt) 2020-07-01
WO2016005931A1 (en) 2016-01-14
JP6748061B2 (ja) 2020-08-26

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