MX2018010108A - Sistema de amplificacion por reaccion en cadena de la polimerasa mediada por luz y de deteccion de productos y metodos de uso. - Google Patents
Sistema de amplificacion por reaccion en cadena de la polimerasa mediada por luz y de deteccion de productos y metodos de uso.Info
- Publication number
- MX2018010108A MX2018010108A MX2018010108A MX2018010108A MX2018010108A MX 2018010108 A MX2018010108 A MX 2018010108A MX 2018010108 A MX2018010108 A MX 2018010108A MX 2018010108 A MX2018010108 A MX 2018010108A MX 2018010108 A MX2018010108 A MX 2018010108A
- Authority
- MX
- Mexico
- Prior art keywords
- methods
- detection system
- product detection
- light
- chain reaction
- Prior art date
Links
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
- B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
- B01L2200/142—Preventing evaporation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0812—Bands; Tapes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
- B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1838—Means for temperature control using fluid heat transfer medium
- B01L2300/1844—Means for temperature control using fluid heat transfer medium using fans
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1861—Means for temperature control using radiation
- B01L2300/1872—Infrared light
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6484—Optical fibres
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/08—Optical fibres; light guides
- G01N2201/0826—Fibre array at source, distributing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/08—Optical fibres; light guides
- G01N2201/0833—Fibre array at detector, resolving
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/021—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a flexible chain, e.g. "cartridge belt", conveyor for reaction cells or cuvettes
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Organic Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Clinical Laboratory Science (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Optics & Photonics (AREA)
- Biophysics (AREA)
- Hematology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Peptides Or Proteins (AREA)
- Dispersion Chemistry (AREA)
Abstract
Se divulga un sistema de amplificación por PCR y de detección de productos. El sistema utiliza un subsistema de calentamiento fotónico uniforme y directo para mediar la amplificación por PCR de alto rendimiento, de reacción en reacción, detectable por un subsistema de detección de fluorescencia. También se divulga el control de la temperatura de reacción en reacción para la regulación del calor por retroalimentación dinámica. También se divulgan métodos para usar el mismo.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201662306432P | 2016-03-10 | 2016-03-10 | |
PCT/US2017/021359 WO2017156126A1 (en) | 2016-03-10 | 2017-03-08 | Light-mediated polymerase chain reaction amplification and product detection system and methods of use |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2018010108A true MX2018010108A (es) | 2018-12-17 |
Family
ID=58361164
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2018010108A MX2018010108A (es) | 2016-03-10 | 2017-03-08 | Sistema de amplificacion por reaccion en cadena de la polimerasa mediada por luz y de deteccion de productos y metodos de uso. |
Country Status (9)
Country | Link |
---|---|
US (1) | US11369965B2 (es) |
EP (1) | EP3426399B1 (es) |
CN (1) | CN108778511A (es) |
BR (1) | BR112018068139B1 (es) |
CA (1) | CA3009080C (es) |
ES (1) | ES2871861T3 (es) |
MX (1) | MX2018010108A (es) |
SG (1) | SG11201805166PA (es) |
WO (1) | WO2017156126A1 (es) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10465495B2 (en) * | 2017-10-02 | 2019-11-05 | Petrolog Automation, Inc | Polished-rod thermal imaging system for preventing failures in the operation of a sucker rod pump |
US11224873B2 (en) | 2018-03-15 | 2022-01-18 | Kryptos Biotechnologies, Inc. | Microfluidic system incorporating light absorbing materials |
US11628442B2 (en) | 2018-10-16 | 2023-04-18 | Kryptos Biotechnologies, Inc. | Method and system for reaction vessel with multisided energy sources |
US11648563B2 (en) * | 2018-12-21 | 2023-05-16 | Kryptos Biotechnologies, Inc. | Method and system for heating and temperature measurement using patterned thin films |
GB2586453B (en) * | 2019-08-13 | 2024-04-10 | Causeway Sensors Ltd | Analyzing system for multi-well sample carriers |
CN116438444A (zh) * | 2020-09-04 | 2023-07-14 | 波士顿大学理事会 | 荧光编码的中红外光热显微镜 |
CN112285108B (zh) * | 2020-10-20 | 2023-11-21 | 深圳技术大学 | 一种基于红外热成像技术的便携式贵金属层间掺假无损检测装置 |
WO2023154897A1 (en) * | 2022-02-11 | 2023-08-17 | Singular Genomics Systems, Inc. | Nucleic acid amplification and methylation pattern retention |
CN116731841A (zh) * | 2022-11-10 | 2023-09-12 | 南方科技大学 | 微针加热扩增结构、快速核酸检测芯片、装置与方法 |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7081226B1 (en) | 1996-06-04 | 2006-07-25 | University Of Utah Research Foundation | System and method for fluorescence monitoring |
WO1996041864A1 (en) | 1995-06-13 | 1996-12-27 | The Regents Of The University Of California | Diode laser heated micro-reaction chamber with sample detection means |
PT912766E (pt) | 1996-06-04 | 2009-07-16 | Univ Utah Res Found | Monitorização da hibridização durante a pcr |
US5972667A (en) * | 1998-05-19 | 1999-10-26 | Cell Robotics, Inc. | Method and apparatus for activating a thermo-enzyme reaction with electromagnetic energy |
US6783993B1 (en) * | 1999-03-25 | 2004-08-31 | Alphahelix Ab | Homogenizing of small-volume mixtures by centrifugation and heating |
US6720187B2 (en) * | 2000-06-28 | 2004-04-13 | 3M Innovative Properties Company | Multi-format sample processing devices |
US7232547B2 (en) * | 2002-03-22 | 2007-06-19 | Marshfield Clinic | Apparatus and method for testing and continuously reading low-volume samples |
US6833536B2 (en) * | 2002-05-22 | 2004-12-21 | Applera Corporation | Non-contact radiant heating and temperature sensing device for a chemical reaction chamber |
EP1608952B1 (en) * | 2002-12-20 | 2016-08-10 | Life Technologies Corporation | Assay apparatus and method using microfluidic arrays |
EP1541237A3 (en) | 2003-12-10 | 2006-02-01 | Samsung Electronics Co., Ltd. | Polymerase chain reaction (pcr) module and multiple pcr system using the same |
US7122799B2 (en) * | 2003-12-18 | 2006-10-17 | Palo Alto Research Center Incorporated | LED or laser enabled real-time PCR system and spectrophotometer |
JP2009540290A (ja) * | 2006-06-08 | 2009-11-19 | コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ | Dna検出用ミクロ電子工学センサ装置 |
CA2677833C (en) * | 2007-01-22 | 2016-05-03 | Wafergen, Inc. | Apparatus for high throughput chemical reactions |
US9475051B2 (en) * | 2007-02-27 | 2016-10-25 | Sony Corporation | Nucleic acid amplifier |
JP5279481B2 (ja) * | 2008-12-25 | 2013-09-04 | 株式会社日立ハイテクノロジーズ | 核酸分析装置 |
CN103153466B (zh) * | 2010-07-22 | 2016-04-13 | 基因细胞生物系统有限公司 | 复合液体池 |
-
2017
- 2017-03-08 EP EP17712360.1A patent/EP3426399B1/en active Active
- 2017-03-08 MX MX2018010108A patent/MX2018010108A/es unknown
- 2017-03-08 ES ES17712360T patent/ES2871861T3/es active Active
- 2017-03-08 BR BR112018068139-3A patent/BR112018068139B1/pt active IP Right Grant
- 2017-03-08 US US16/061,590 patent/US11369965B2/en active Active
- 2017-03-08 CN CN201780016265.2A patent/CN108778511A/zh active Pending
- 2017-03-08 SG SG11201805166PA patent/SG11201805166PA/en unknown
- 2017-03-08 CA CA3009080A patent/CA3009080C/en active Active
- 2017-03-08 WO PCT/US2017/021359 patent/WO2017156126A1/en active Application Filing
Also Published As
Publication number | Publication date |
---|---|
SG11201805166PA (en) | 2018-07-30 |
ES2871861T3 (es) | 2021-11-02 |
US11369965B2 (en) | 2022-06-28 |
CN108778511A (zh) | 2018-11-09 |
US20180361379A1 (en) | 2018-12-20 |
CA3009080C (en) | 2023-10-10 |
EP3426399A1 (en) | 2019-01-16 |
BR112018068139A2 (pt) | 2019-01-15 |
WO2017156126A1 (en) | 2017-09-14 |
EP3426399B1 (en) | 2021-03-10 |
BR112018068139B1 (pt) | 2022-12-06 |
CA3009080A1 (en) | 2017-09-14 |
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