SG11201805166PA - Light-mediated polymerase chain reaction amplification and product detection system and methods of use - Google Patents

Light-mediated polymerase chain reaction amplification and product detection system and methods of use

Info

Publication number
SG11201805166PA
SG11201805166PA SG11201805166PA SG11201805166PA SG11201805166PA SG 11201805166P A SG11201805166P A SG 11201805166PA SG 11201805166P A SG11201805166P A SG 11201805166PA SG 11201805166P A SG11201805166P A SG 11201805166PA SG 11201805166P A SG11201805166P A SG 11201805166PA
Authority
SG
Singapore
Prior art keywords
international
iowa
reaction
rule
methods
Prior art date
Application number
SG11201805166PA
Inventor
Ronald L Biro
Beau Hunt
Original Assignee
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int filed Critical Pioneer Hi Bred Int
Publication of SG11201805166PA publication Critical patent/SG11201805166PA/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/021—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a flexible chain, e.g. "cartridge belt", conveyor for reaction cells or cuvettes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/142—Preventing evaporation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0812—Bands; Tapes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1838—Means for temperature control using fluid heat transfer medium
    • B01L2300/1844—Means for temperature control using fluid heat transfer medium using fans
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1861—Means for temperature control using radiation
    • B01L2300/1872—Infrared light
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6484—Optical fibres
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/08—Optical fibres; light guides
    • G01N2201/0826—Fibre array at source, distributing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/08—Optical fibres; light guides
    • G01N2201/0833—Fibre array at detector, resolving

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Clinical Laboratory Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Optics & Photonics (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Peptides Or Proteins (AREA)
  • Dispersion Chemistry (AREA)

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization 111111110111101110101011111010111110111011111011011011101111101111101111011111 International Bureau .. .... ..Yejd (10) International Publication Number (43) International Publication Date .... ...,,, WO 2017/156126 Al 14 September 2017 (14.09.2017) WIP0 I PCT (51) International Patent Classification: BOIL 3/00 (2006.01) G01N 21/64 (2006.01) BOIL 7/00 (2006.01) G01N 35/02 (2006.01) KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, (21) International Application Number: PCT/US2017/021359 TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (22) International Filing Date: (84) Designated States (unless otherwise indicated, for every 8 March 2017 (08.03.2017) kind of regional protection available): ARIPO (BW, GH, (25) Filing Language: English GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, (26) Publication Language: English TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, (30) Priority Data: 62/306,432 10 March 2016 (10.03.2016) US DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, (71) Applicant: PIONEER HI-BRED INTERNATIONAL, INC. [US/US]; 7100 NW 62ND AVE, PO BOX 1014, Johnston, Iowa 50131-1014 (US). GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: (72) Inventors: BIRO, Ronald L; 7250 NW 62nd Avenue, PO as to applicant's entitlement to apply for and be granted a Box 552, Johnston, Iowa 50131-0552 (US). HUNT, Beau; patent (Rule 4.17(u)) 1010 NE 5th Street, Ankeny, Iowa 50021-2065 (US). — as to the applicant's entitlement to claim the priority of the _ Agent: STIMSON, Michael J.; 7250 NW 62ND AVE, (74) earlier application (Rule 4.17(iii)) PO BOX 552, Johnston, Iowa 50131-0552 (US). Published: Designated States (unless otherwise indicated, for every (81) — with international search report (Art. 21(3)) = kind of national protection available): AE, AG, AL, AM, _ before the expiration of the time limit for amending the AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) — = _ = = = = = = = = = 1-1 ,tD ei ,-1 ,tD kin ,-1 (54) Title: LIGHT-MEDIATED POLYMERASE CHAIN REACTION AMPLIFICATION AND PRODUCT DETECTION SYS - 11 TEM AND METHODS OF USE 0 N (57) : A PCR amplification and product detection system is disclosed. The system utilizes a uniform and direct photonic en. ) heating subsystem to mediate reaction-by-reaction, high-throughput PCR amplification detectable by a fluorescence detection sub - system. Reaction-by-reaction temperature monitoring for dynamic feedback heat regulation is also disclosed. Also disclosed are methods for using the same.
SG11201805166PA 2016-03-10 2017-03-08 Light-mediated polymerase chain reaction amplification and product detection system and methods of use SG11201805166PA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662306432P 2016-03-10 2016-03-10
PCT/US2017/021359 WO2017156126A1 (en) 2016-03-10 2017-03-08 Light-mediated polymerase chain reaction amplification and product detection system and methods of use

Publications (1)

Publication Number Publication Date
SG11201805166PA true SG11201805166PA (en) 2018-07-30

Family

ID=58361164

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201805166PA SG11201805166PA (en) 2016-03-10 2017-03-08 Light-mediated polymerase chain reaction amplification and product detection system and methods of use

Country Status (9)

Country Link
US (1) US11369965B2 (en)
EP (1) EP3426399B1 (en)
CN (1) CN108778511A (en)
BR (1) BR112018068139B1 (en)
CA (1) CA3009080C (en)
ES (1) ES2871861T3 (en)
MX (1) MX2018010108A (en)
SG (1) SG11201805166PA (en)
WO (1) WO2017156126A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10465495B2 (en) * 2017-10-02 2019-11-05 Petrolog Automation, Inc Polished-rod thermal imaging system for preventing failures in the operation of a sucker rod pump
US11298698B2 (en) 2018-03-15 2022-04-12 Kryptos Biotechnologies, Inc. Method and system for performing heat assisted biochemical reactions
JP2022504858A (en) * 2018-10-16 2022-01-13 クリプトス バイオテクノロジーズ，インコーポレイテッド Methods and systems for temperature monitoring of biochemical reaction vessels
EP3899401A4 (en) * 2018-12-21 2023-01-18 Kryptos Biotechnologies, Inc. Method and system for heating and temperature measurement using patterned thin films
GB2586453B (en) * 2019-08-13 2024-04-10 Causeway Sensors Ltd Analyzing system for multi-well sample carriers
CN116438444A (en) 2020-09-04 2023-07-14 波士顿大学理事会 Fluorescence coded mid-infrared thermal microscope
CN112285108B (en) * 2020-10-20 2023-11-21 深圳技术大学 Portable noble metal interlayer adulteration nondestructive test device based on infrared thermal imaging technology
WO2023154897A1 (en) * 2022-02-11 2023-08-17 Singular Genomics Systems, Inc. Nucleic acid amplification and methylation pattern retention
CN116731841A (en) * 2022-11-10 2023-09-12 南方科技大学 Microneedle heating amplification structure, rapid nucleic acid detection chip, device and method

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US7081226B1 (en) * 1996-06-04 2006-07-25 University Of Utah Research Foundation System and method for fluorescence monitoring
WO1996041864A1 (en) * 1995-06-13 1996-12-27 The Regents Of The University Of California Diode laser heated micro-reaction chamber with sample detection means
DE69733282T2 (en) 1996-06-04 2006-01-19 University Of Utah Research Foundation, Salt Lake City Monitoring hybridization during PCR
US5972667A (en) * 1998-05-19 1999-10-26 Cell Robotics, Inc. Method and apparatus for activating a thermo-enzyme reaction with electromagnetic energy
AU766869B2 (en) * 1999-03-25 2003-10-23 Alphahelix Ab Homogenising of small-volume mixtures by centrifugation and heating
US6720187B2 (en) * 2000-06-28 2004-04-13 3M Innovative Properties Company Multi-format sample processing devices
US7232547B2 (en) * 2002-03-22 2007-06-19 Marshfield Clinic Apparatus and method for testing and continuously reading low-volume samples
US6833536B2 (en) * 2002-05-22 2004-12-21 Applera Corporation Non-contact radiant heating and temperature sensing device for a chemical reaction chamber
WO2004074818A2 (en) * 2002-12-20 2004-09-02 Biotrove, Inc. Assay apparatus and method using microfluidic arrays
EP1541237A3 (en) 2003-12-10 2006-02-01 Samsung Electronics Co., Ltd. Polymerase chain reaction (pcr) module and multiple pcr system using the same
US7122799B2 (en) * 2003-12-18 2006-10-17 Palo Alto Research Center Incorporated LED or laser enabled real-time PCR system and spectrophotometer
RU2008148143A (en) * 2006-06-08 2010-06-10 Конинклейке Филипс Электроникс Н.В. (Nl) MICROELECTRONIC SENSOR DEVICE FOR DNA DETECTION
WO2008091626A1 (en) * 2007-01-22 2008-07-31 Wafergen, Inc. Apparatus for high throughput chemical reactions
US9475051B2 (en) * 2007-02-27 2016-10-25 Sony Corporation Nucleic acid amplifier
JP5279481B2 (en) * 2008-12-25 2013-09-04 株式会社日立ハイテクノロジーズ Nucleic acid analyzer
JP5893620B2 (en) * 2010-07-22 2016-03-23 ジェンセル バイオシステムズ リミテッド Synthetic liquid cell

Also Published As

Publication number Publication date
MX2018010108A (en) 2018-12-17
CA3009080A1 (en) 2017-09-14
CN108778511A (en) 2018-11-09
US11369965B2 (en) 2022-06-28
EP3426399A1 (en) 2019-01-16
WO2017156126A1 (en) 2017-09-14
BR112018068139B1 (en) 2022-12-06
BR112018068139A2 (en) 2019-01-15
ES2871861T3 (en) 2021-11-02
CA3009080C (en) 2023-10-10
EP3426399B1 (en) 2021-03-10
US20180361379A1 (en) 2018-12-20

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