BR112018068139A2 - ?sistema de detecção de produto e amplificação de reação em cadeia da polimerase (pcr) e método de detecção de produto e amplificação de pcr mediada por luz? - Google Patents
?sistema de detecção de produto e amplificação de reação em cadeia da polimerase (pcr) e método de detecção de produto e amplificação de pcr mediada por luz?Info
- Publication number
- BR112018068139A2 BR112018068139A2 BR112018068139A BR112018068139A BR112018068139A2 BR 112018068139 A2 BR112018068139 A2 BR 112018068139A2 BR 112018068139 A BR112018068139 A BR 112018068139A BR 112018068139 A BR112018068139 A BR 112018068139A BR 112018068139 A2 BR112018068139 A2 BR 112018068139A2
- Authority
- BR
- Brazil
- Prior art keywords
- product detection
- pcr
- amplification
- light
- chain reaction
- Prior art date
Links
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
- B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
- B01L2200/142—Preventing evaporation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0812—Bands; Tapes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
- B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1838—Means for temperature control using fluid heat transfer medium
- B01L2300/1844—Means for temperature control using fluid heat transfer medium using fans
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1861—Means for temperature control using radiation
- B01L2300/1872—Infrared light
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6484—Optical fibres
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/08—Optical fibres; light guides
- G01N2201/0826—Fibre array at source, distributing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/08—Optical fibres; light guides
- G01N2201/0833—Fibre array at detector, resolving
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/021—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a flexible chain, e.g. "cartridge belt", conveyor for reaction cells or cuvettes
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Organic Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Clinical Laboratory Science (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Hematology (AREA)
- Biophysics (AREA)
- Optics & Photonics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Peptides Or Proteins (AREA)
- Dispersion Chemistry (AREA)
Abstract
a presente invenção refere-se a um sistema de detecção de produto e amplificação de pcr. o sistema utiliza um subsistema de aquecimento fotônico uniforme e direto para mediar a amplificação de pcr de alto rendimento de reação em reação, detectável por um subsistema de detecção de fluorescência. também é revelado o monitoramento de temperatura de reação em reação para regulação térmica de retroalimentação dinâmica. também são revelados métodos para usar os mesmos.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201662306432P | 2016-03-10 | 2016-03-10 | |
US62/306,432 | 2016-03-10 | ||
PCT/US2017/021359 WO2017156126A1 (en) | 2016-03-10 | 2017-03-08 | Light-mediated polymerase chain reaction amplification and product detection system and methods of use |
Publications (2)
Publication Number | Publication Date |
---|---|
BR112018068139A2 true BR112018068139A2 (pt) | 2019-01-15 |
BR112018068139B1 BR112018068139B1 (pt) | 2022-12-06 |
Family
ID=58361164
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112018068139-3A BR112018068139B1 (pt) | 2016-03-10 | 2017-03-08 | Sistema de detecção de produto e amplificação de reação em cadeia da polimerase (pcr) e método de detecção de produto e amplificação de pcr mediada por luz |
Country Status (9)
Country | Link |
---|---|
US (1) | US11369965B2 (pt) |
EP (1) | EP3426399B1 (pt) |
CN (1) | CN108778511A (pt) |
BR (1) | BR112018068139B1 (pt) |
CA (1) | CA3009080C (pt) |
ES (1) | ES2871861T3 (pt) |
MX (1) | MX2018010108A (pt) |
SG (1) | SG11201805166PA (pt) |
WO (1) | WO2017156126A1 (pt) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10465495B2 (en) * | 2017-10-02 | 2019-11-05 | Petrolog Automation, Inc | Polished-rod thermal imaging system for preventing failures in the operation of a sucker rod pump |
US11298698B2 (en) | 2018-03-15 | 2022-04-12 | Kryptos Biotechnologies, Inc. | Method and system for performing heat assisted biochemical reactions |
JP2022504858A (ja) * | 2018-10-16 | 2022-01-13 | クリプトス バイオテクノロジーズ,インコーポレイテッド | 生化学反応容器の温度監視のための方法およびシステム |
US11648563B2 (en) * | 2018-12-21 | 2023-05-16 | Kryptos Biotechnologies, Inc. | Method and system for heating and temperature measurement using patterned thin films |
GB2586453B (en) * | 2019-08-13 | 2024-04-10 | Causeway Sensors Ltd | Analyzing system for multi-well sample carriers |
US11561179B2 (en) | 2020-09-04 | 2023-01-24 | Trustees Of Boston University | Fluorescence-coded mid-infrared photothermal microscope |
CN112285108B (zh) * | 2020-10-20 | 2023-11-21 | 深圳技术大学 | 一种基于红外热成像技术的便携式贵金属层间掺假无损检测装置 |
WO2023154897A1 (en) * | 2022-02-11 | 2023-08-17 | Singular Genomics Systems, Inc. | Nucleic acid amplification and methylation pattern retention |
US20230301354A1 (en) * | 2022-03-24 | 2023-09-28 | Derek Kyle King | Quantum Lit Vaping System |
CN116731841A (zh) * | 2022-11-10 | 2023-09-12 | 南方科技大学 | 微针加热扩增结构、快速核酸检测芯片、装置与方法 |
Family Cites Families (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US7081226B1 (en) * | 1996-06-04 | 2006-07-25 | University Of Utah Research Foundation | System and method for fluorescence monitoring |
WO1996041864A1 (en) | 1995-06-13 | 1996-12-27 | The Regents Of The University Of California | Diode laser heated micro-reaction chamber with sample detection means |
CA2257109C (en) | 1996-06-04 | 2009-10-06 | University Of Utah Research Foundation | Monitoring hybridization during pcr |
US5972667A (en) * | 1998-05-19 | 1999-10-26 | Cell Robotics, Inc. | Method and apparatus for activating a thermo-enzyme reaction with electromagnetic energy |
AU766869B2 (en) * | 1999-03-25 | 2003-10-23 | Alphahelix Ab | Homogenising of small-volume mixtures by centrifugation and heating |
US6720187B2 (en) * | 2000-06-28 | 2004-04-13 | 3M Innovative Properties Company | Multi-format sample processing devices |
US7232547B2 (en) * | 2002-03-22 | 2007-06-19 | Marshfield Clinic | Apparatus and method for testing and continuously reading low-volume samples |
US6833536B2 (en) * | 2002-05-22 | 2004-12-21 | Applera Corporation | Non-contact radiant heating and temperature sensing device for a chemical reaction chamber |
CA2521999A1 (en) * | 2002-12-20 | 2004-09-02 | Biotrove, Inc. | Assay apparatus and method using microfluidic arrays |
EP1541237A3 (en) | 2003-12-10 | 2006-02-01 | Samsung Electronics Co., Ltd. | Polymerase chain reaction (pcr) module and multiple pcr system using the same |
US7122799B2 (en) * | 2003-12-18 | 2006-10-17 | Palo Alto Research Center Incorporated | LED or laser enabled real-time PCR system and spectrophotometer |
RU2008148143A (ru) * | 2006-06-08 | 2010-06-10 | Конинклейке Филипс Электроникс Н.В. (Nl) | Микроэлектронное сенсорное устройство для детектирования днк |
CN101711257A (zh) * | 2007-01-22 | 2010-05-19 | 瓦弗根公司 | 用于高通量化学反应的装置 |
US9475051B2 (en) | 2007-02-27 | 2016-10-25 | Sony Corporation | Nucleic acid amplifier |
JP5279481B2 (ja) * | 2008-12-25 | 2013-09-04 | 株式会社日立ハイテクノロジーズ | 核酸分析装置 |
WO2012011091A2 (en) | 2010-07-22 | 2012-01-26 | Gencell Biosystems Limited | Composite liquid cells |
-
2017
- 2017-03-08 ES ES17712360T patent/ES2871861T3/es active Active
- 2017-03-08 WO PCT/US2017/021359 patent/WO2017156126A1/en active Application Filing
- 2017-03-08 CN CN201780016265.2A patent/CN108778511A/zh active Pending
- 2017-03-08 US US16/061,590 patent/US11369965B2/en active Active
- 2017-03-08 SG SG11201805166PA patent/SG11201805166PA/en unknown
- 2017-03-08 CA CA3009080A patent/CA3009080C/en active Active
- 2017-03-08 MX MX2018010108A patent/MX2018010108A/es unknown
- 2017-03-08 BR BR112018068139-3A patent/BR112018068139B1/pt active IP Right Grant
- 2017-03-08 EP EP17712360.1A patent/EP3426399B1/en active Active
Also Published As
Publication number | Publication date |
---|---|
SG11201805166PA (en) | 2018-07-30 |
US20180361379A1 (en) | 2018-12-20 |
WO2017156126A1 (en) | 2017-09-14 |
CN108778511A (zh) | 2018-11-09 |
MX2018010108A (es) | 2018-12-17 |
EP3426399B1 (en) | 2021-03-10 |
CA3009080A1 (en) | 2017-09-14 |
CA3009080C (en) | 2023-10-10 |
BR112018068139B1 (pt) | 2022-12-06 |
US11369965B2 (en) | 2022-06-28 |
ES2871861T3 (es) | 2021-11-02 |
EP3426399A1 (en) | 2019-01-16 |
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Legal Events
Date | Code | Title | Description |
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B06W | Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette] | ||
B350 | Update of information on the portal [chapter 15.35 patent gazette] | ||
B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 08/03/2017, OBSERVADAS AS CONDICOES LEGAIS |