MX2017011195A - Metodos para mejorar la captacion especifica de neurotoxinas botulinicas en las celulas. - Google Patents
Metodos para mejorar la captacion especifica de neurotoxinas botulinicas en las celulas.Info
- Publication number
- MX2017011195A MX2017011195A MX2017011195A MX2017011195A MX2017011195A MX 2017011195 A MX2017011195 A MX 2017011195A MX 2017011195 A MX2017011195 A MX 2017011195A MX 2017011195 A MX2017011195 A MX 2017011195A MX 2017011195 A MX2017011195 A MX 2017011195A
- Authority
- MX
- Mexico
- Prior art keywords
- cells
- neurotoxin polypeptide
- neurotoxin
- detection
- allow
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 230000002708 enhancing effect Effects 0.000 title abstract 3
- 108030001720 Bontoxilysin Proteins 0.000 title 1
- 231100001103 botulinum neurotoxin Toxicity 0.000 title 1
- 101710138657 Neurotoxin Proteins 0.000 abstract 15
- 239000002581 neurotoxin Substances 0.000 abstract 15
- 231100000618 neurotoxin Toxicity 0.000 abstract 15
- 229920001184 polypeptide Polymers 0.000 abstract 13
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 13
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 13
- 238000001514 detection method Methods 0.000 abstract 8
- 230000004071 biological effect Effects 0.000 abstract 4
- 239000000758 substrate Substances 0.000 abstract 4
- 238000013019 agitation Methods 0.000 abstract 2
- 238000011534 incubation Methods 0.000 abstract 2
- 230000035987 intoxication Effects 0.000 abstract 2
- 231100000566 intoxication Toxicity 0.000 abstract 2
- 230000001404 mediated effect Effects 0.000 abstract 2
- 230000028161 membrane depolarization Effects 0.000 abstract 2
- 238000003776 cleavage reaction Methods 0.000 abstract 1
- 239000003599 detergent Substances 0.000 abstract 1
- 230000007017 scission Effects 0.000 abstract 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
- G01N33/5058—Neurological cells
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- A—HUMAN NECESSITIES
- A47—FURNITURE; DOMESTIC ARTICLES OR APPLIANCES; COFFEE MILLS; SPICE MILLS; SUCTION CLEANERS IN GENERAL
- A47L—DOMESTIC WASHING OR CLEANING; SUCTION CLEANERS IN GENERAL
- A47L9/00—Details or accessories of suction cleaners, e.g. mechanical means for controlling the suction or for effecting pulsating action; Storing devices specially adapted to suction cleaners or parts thereof; Carrying-vehicles specially adapted for suction cleaners
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09J—ADHESIVES; NON-MECHANICAL ASPECTS OF ADHESIVE PROCESSES IN GENERAL; ADHESIVE PROCESSES NOT PROVIDED FOR ELSEWHERE; USE OF MATERIALS AS ADHESIVES
- C09J7/00—Adhesives in the form of films or foils
- C09J7/40—Adhesives in the form of films or foils characterised by release liners
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0618—Cells of the nervous system
- C12N5/0619—Neurons
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B5/00—Optical elements other than lenses
- G02B5/30—Polarising elements
-
- G—PHYSICS
- G02—OPTICS
- G02F—OPTICAL DEVICES OR ARRANGEMENTS FOR THE CONTROL OF LIGHT BY MODIFICATION OF THE OPTICAL PROPERTIES OF THE MEDIA OF THE ELEMENTS INVOLVED THEREIN; NON-LINEAR OPTICS; FREQUENCY-CHANGING OF LIGHT; OPTICAL LOGIC ELEMENTS; OPTICAL ANALOGUE/DIGITAL CONVERTERS
- G02F1/00—Devices or arrangements for the control of the intensity, colour, phase, polarisation or direction of light arriving from an independent light source, e.g. switching, gating or modulating; Non-linear optics
- G02F1/01—Devices or arrangements for the control of the intensity, colour, phase, polarisation or direction of light arriving from an independent light source, e.g. switching, gating or modulating; Non-linear optics for the control of the intensity, phase, polarisation or colour
- G02F1/13—Devices or arrangements for the control of the intensity, colour, phase, polarisation or direction of light arriving from an independent light source, e.g. switching, gating or modulating; Non-linear optics for the control of the intensity, phase, polarisation or colour based on liquid crystals, e.g. single liquid crystal display cells
- G02F1/133—Constructional arrangements; Operation of liquid crystal cells; Circuit arrangements
- G02F1/1333—Constructional arrangements; Manufacturing methods
- G02F1/1335—Structural association of cells with optical devices, e.g. polarisers or reflectors
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B32—LAYERED PRODUCTS
- B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- B32B38/00—Ancillary operations in connection with laminating processes
- B32B38/10—Removing layers, or parts of layers, mechanically or chemically
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B32—LAYERED PRODUCTS
- B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- B32B43/00—Operations specially adapted for layered products and not otherwise provided for, e.g. repairing; Apparatus therefor
- B32B43/006—Delaminating
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/33—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Clostridium (G)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T156/00—Adhesive bonding and miscellaneous chemical manufacture
- Y10T156/11—Methods of delaminating, per se; i.e., separating at bonding face
- Y10T156/1126—Using direct fluid current against work during delaminating
- Y10T156/1132—Using vacuum directly against work during delaminating
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T156/00—Adhesive bonding and miscellaneous chemical manufacture
- Y10T156/11—Methods of delaminating, per se; i.e., separating at bonding face
- Y10T156/1168—Gripping and pulling work apart during delaminating
- Y10T156/1174—Using roller for delamination [e.g., roller pairs operating at differing speeds or directions, etc.]
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T156/00—Adhesive bonding and miscellaneous chemical manufacture
- Y10T156/11—Methods of delaminating, per se; i.e., separating at bonding face
- Y10T156/1168—Gripping and pulling work apart during delaminating
- Y10T156/1195—Delaminating from release surface
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T156/00—Adhesive bonding and miscellaneous chemical manufacture
- Y10T156/19—Delaminating means
- Y10T156/1928—Differential fluid pressure delaminating means
- Y10T156/1944—Vacuum delaminating means [e.g., vacuum chamber, etc.]
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T156/00—Adhesive bonding and miscellaneous chemical manufacture
- Y10T156/19—Delaminating means
- Y10T156/195—Delaminating roller means
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T156/00—Adhesive bonding and miscellaneous chemical manufacture
- Y10T156/19—Delaminating means
- Y10T156/1994—Means for delaminating from release surface
Abstract
La presente invención proporciona un método para mejorar la captación específica de un polipéptido de neurotoxina en las células, el método comprende: incubar células susceptibles a la intoxicación por neurotoxina con un polipéptido de neurotoxina durante un tiempo y en condiciones que permiten al polipéptido de neurotoxina ejercer su actividad biológica, la incubación comprende al menos una de las siguientes etapas: (i) despolarización de las células mediada por K+, (ii) un tiempo de exposición al polipéptido de neurotoxina reducido y/o (iii) agitación de las células durante la exposición al polipéptido de neurotoxina, para mejorar de esta manera la captación específica del polipéptido de neurotoxina en las células. Además, la invención se refiere a un método para determinar directamente la actividad biológica de un polipéptido de neurotoxina en las células, que comprende: a) incubar células susceptibles a la intoxicación por neurotoxina con un polipéptido de neurotoxina durante un tiempo y en condiciones que permiten al polipéptido de neurotoxina ejercer su actividad biológica, la incubación comprende al menos una de las siguientes etapas: (i) despolarización de las células mediada por K, (ii) un tiempo de exposición al polipéptido de neurotoxina reducido y/o (iii) agitación de las células durante la exposición al polipéptido de neurotoxina; b) fijar las células y, opcionalmente, permeabilizar las células con un detergente; c) poner en contacto las células con al menos un primer anticuerpo de captura que se une específicamente al sustrato no escindido y al escindido por neurotoxina y con al menos un segundo anticuerpo de captura que se une específicamente al sitio de escisión del sustrato escindido por neurotoxina, en condiciones que permiten la unión de los anticuerpos de captura a los sustratos; d) poner en contacto las células con al menos un primer anticuerpo de detección que se une específicamente al primer anticuerpo de captura, en condiciones que permiten la unión del primer anticuerpo de detección a el primer anticuerpo de captura, para formar así los primeros complejos de detección y con al menos un segundo anticuerpo de detección que se une específicamente al segundo anticuerpo de captura, en condiciones que permiten la unión del segundo anticuerpo de detección a el segundo anticuerpo de captura, para formar así los segundos complejos de detección; e) determinar la cantidad del primer y segundo complejos de detección de la etapa d); y f) calcular la cantidad de sustrato escindido por el polipéptido de neurotoxina en las células por medio de los segundos complejos de detección, para determinar de esta manera la actividad biológica del polipéptido de neurotoxina en las célula.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP15157549 | 2015-03-04 | ||
PCT/EP2016/054552 WO2016139308A1 (en) | 2015-03-04 | 2016-03-03 | Methods for enhancing the specific uptake of botulinum neurotoxins into cells |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2017011195A true MX2017011195A (es) | 2017-11-09 |
Family
ID=52705963
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2017011195A MX2017011195A (es) | 2015-03-04 | 2016-03-03 | Metodos para mejorar la captacion especifica de neurotoxinas botulinicas en las celulas. |
Country Status (16)
Country | Link |
---|---|
US (1) | US10725025B2 (es) |
EP (1) | EP3265808B1 (es) |
JP (1) | JP6670842B2 (es) |
KR (1) | KR102418939B1 (es) |
CN (1) | CN108064310B (es) |
AU (1) | AU2016227655B2 (es) |
BR (1) | BR112017018962A2 (es) |
CA (1) | CA2978225C (es) |
ES (1) | ES2822905T3 (es) |
HK (1) | HK1249147A1 (es) |
IL (1) | IL254181B (es) |
MX (1) | MX2017011195A (es) |
RU (1) | RU2684404C2 (es) |
SG (1) | SG11201707157PA (es) |
TW (1) | TWI704156B (es) |
WO (1) | WO2016139308A1 (es) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NZ588029A (en) * | 2008-03-14 | 2012-12-21 | Allergan Inc | Immuno-based botulinum toxin serotype a activity assays |
JP6608357B2 (ja) | 2013-06-28 | 2019-11-20 | メルツ ファルマ ゲーエムベーハー ウント コンパニー カーゲーアーアー | 細胞において神経毒ポリペプチドの生物活性を決定する手段及び方法 |
TWI704156B (zh) * | 2015-03-04 | 2020-09-11 | 德商曼茲法瑪股份有限公司 | 用於增進肉毒桿菌神經毒素進入細胞之特定攝取的方法 |
CN115826239A (zh) | 2017-10-16 | 2023-03-21 | 奥里姆光学有限公司 | 高效紧凑型头戴式显示系统 |
EP3752128A1 (de) * | 2018-02-16 | 2020-12-23 | Bontana Therapies Gmbh | Nukleinsäure-basiertes botulinum neurotoxin zur therapeutischen anwendung |
CN111621525B (zh) * | 2020-06-18 | 2021-04-23 | 中赛干细胞基因工程有限公司 | Stx1b基因在促进人脂肪间充质干细胞生长和分化中的用途 |
Family Cites Families (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE60032367T3 (de) * | 1999-08-25 | 2011-03-10 | Allergan, Inc., Irvine | Aktivierbare rekombinante neurotoxine |
US20080032931A1 (en) * | 1999-08-25 | 2008-02-07 | Steward Lance E | Activatable clostridial toxins |
FI20070808A0 (fi) * | 2007-10-25 | 2007-10-25 | Mart Saarma | GDNF:n silmukointivariantit ja niiden käytöt |
NZ588029A (en) * | 2008-03-14 | 2012-12-21 | Allergan Inc | Immuno-based botulinum toxin serotype a activity assays |
WO2010085473A1 (en) * | 2009-01-20 | 2010-07-29 | Trustees Of Tufts College | Methods for the delivery of toxins or enzymatically active portions thereof |
EP2398897B1 (en) | 2009-02-20 | 2017-06-28 | Cellular Dynamics International, Inc. | Methods and compositions for the differentiation of stem cells |
KR101720961B1 (ko) | 2009-02-27 | 2017-03-29 | 셀룰러 다이내믹스 인터내셔널, 인코포레이티드 | 다능성 세포의 분화 |
AU2010223910B2 (en) | 2009-03-13 | 2015-03-12 | Allergan, Inc. | Cells useful for immuno-based Botulinum toxin serotype A activity assays |
WO2011025852A1 (en) | 2009-08-28 | 2011-03-03 | Cellular Dynamics International, Inc. | Identifying genetic variation in affected tissues |
ES2539487T3 (es) | 2009-11-04 | 2015-07-01 | Cellular Dynamics International, Inc. | Reprogramación episómica con compuestos químicos |
CA2780932A1 (en) * | 2009-11-18 | 2011-05-26 | Merz Pharma Gmbh & Co. Kgaa | Assay for quantifying clostridial neurotoxin |
US9487752B2 (en) | 2011-03-30 | 2016-11-08 | Cellular Dynamics International, Inc. | Priming of pluripotent stem cells for neural differentiation |
WO2014079878A1 (en) | 2012-11-21 | 2014-05-30 | Merz Pharma Gmbh & Co. Kgaa | Means and methods for determination of botulinum neurotoxin biological activity |
JP6608357B2 (ja) | 2013-06-28 | 2019-11-20 | メルツ ファルマ ゲーエムベーハー ウント コンパニー カーゲーアーアー | 細胞において神経毒ポリペプチドの生物活性を決定する手段及び方法 |
US10634683B2 (en) * | 2014-11-21 | 2020-04-28 | Merz Pharma Gmbh & Co. Kgaa | Methods for the determination of the biological activities of neurotoxin polypeptides |
CN107207586B (zh) * | 2014-12-19 | 2021-07-13 | 莫茨药物股份两合公司 | 用于测定BoNT/E在细胞中的生物活性的装置和方法 |
TWI704156B (zh) * | 2015-03-04 | 2020-09-11 | 德商曼茲法瑪股份有限公司 | 用於增進肉毒桿菌神經毒素進入細胞之特定攝取的方法 |
-
2016
- 2016-02-24 TW TW105105453A patent/TWI704156B/zh active
- 2016-03-03 SG SG11201707157PA patent/SG11201707157PA/en unknown
- 2016-03-03 RU RU2017130580A patent/RU2684404C2/ru active
- 2016-03-03 KR KR1020177024406A patent/KR102418939B1/ko active IP Right Grant
- 2016-03-03 EP EP16707785.8A patent/EP3265808B1/en active Active
- 2016-03-03 US US15/554,338 patent/US10725025B2/en active Active
- 2016-03-03 MX MX2017011195A patent/MX2017011195A/es unknown
- 2016-03-03 ES ES16707785T patent/ES2822905T3/es active Active
- 2016-03-03 BR BR112017018962-3A patent/BR112017018962A2/pt active Search and Examination
- 2016-03-03 WO PCT/EP2016/054552 patent/WO2016139308A1/en active Application Filing
- 2016-03-03 CN CN201680013526.0A patent/CN108064310B/zh active Active
- 2016-03-03 AU AU2016227655A patent/AU2016227655B2/en active Active
- 2016-03-03 CA CA2978225A patent/CA2978225C/en active Active
- 2016-03-03 JP JP2017546070A patent/JP6670842B2/ja active Active
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2017
- 2017-08-28 IL IL254181A patent/IL254181B/en active IP Right Grant
-
2018
- 2018-07-09 HK HK18108842.3A patent/HK1249147A1/zh unknown
Also Published As
Publication number | Publication date |
---|---|
KR102418939B1 (ko) | 2022-07-07 |
CN108064310A (zh) | 2018-05-22 |
RU2017130580A (ru) | 2019-04-05 |
EP3265808A1 (en) | 2018-01-10 |
AU2016227655B2 (en) | 2021-08-19 |
KR20170121194A (ko) | 2017-11-01 |
WO2016139308A1 (en) | 2016-09-09 |
RU2017130580A3 (es) | 2019-04-05 |
JP2018506985A (ja) | 2018-03-15 |
IL254181A0 (en) | 2017-10-31 |
CA2978225C (en) | 2022-09-13 |
ES2822905T3 (es) | 2021-05-05 |
JP6670842B2 (ja) | 2020-03-25 |
BR112017018962A2 (pt) | 2018-04-24 |
AU2016227655A1 (en) | 2017-09-14 |
IL254181B (en) | 2020-03-31 |
CA2978225A1 (en) | 2016-09-09 |
US10725025B2 (en) | 2020-07-28 |
SG11201707157PA (en) | 2017-09-28 |
RU2684404C2 (ru) | 2019-04-09 |
CN108064310B (zh) | 2021-06-11 |
HK1249147A1 (zh) | 2018-10-26 |
EP3265808B1 (en) | 2020-08-26 |
US20180238861A1 (en) | 2018-08-23 |
TW201639875A (zh) | 2016-11-16 |
TWI704156B (zh) | 2020-09-11 |
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