EA201792296A1 - Электрохимические биосенсоры - Google Patents

Электрохимические биосенсоры

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Publication number
EA201792296A1
EA201792296A1 EA201792296A EA201792296A EA201792296A1 EA 201792296 A1 EA201792296 A1 EA 201792296A1 EA 201792296 A EA201792296 A EA 201792296A EA 201792296 A EA201792296 A EA 201792296A EA 201792296 A1 EA201792296 A1 EA 201792296A1
Authority
EA
Eurasian Patent Office
Prior art keywords
enzyme
electrons
binding
substrate
response
Prior art date
Application number
EA201792296A
Other languages
English (en)
Inventor
Кирилл Александров
Виктор Штейн
Чжун Го
Original Assignee
Зе Юниверсити Оф Квинсленд
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2015902093A external-priority patent/AU2015902093A0/en
Application filed by Зе Юниверсити Оф Квинсленд filed Critical Зе Юниверсити Оф Квинсленд
Publication of EA201792296A1 publication Critical patent/EA201792296A1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C12Q1/006—Enzyme electrodes involving specific analytes or enzymes for glucose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/607—Detection means characterised by use of a special device being a sensor, e.g. electrode
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/99—Oxidoreductases acting on the CH-OH group of donors (1.1) with other acceptors (1.1.99)
    • C12Y101/9901—Glucose dehydrogenase (acceptor) (1.1.99.10)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Emergency Medicine (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Биосенсор, содержащий аминокислотную последовательность фермента, такого как глюкозодегидрогеназа, который способен вступать в реакцию с субстратом с образованием одного или более электронов, причем указанный фермент конструируют так, чтобы он обладал способностью к переключению из каталитически неактивного в каталитически активное состояние в ответ на связывание молекулы-мишени. Предложен способ обнаружения молекулы-мишени, причем фермент, такой как глюкозодегидрогеназа, вступает в реакцию с субстратом с образованием одного или более электронов как результат переключения фермента из каталитически неактивного в каталитически активное состояние в ответ на связывание молекулы-мишени.
EA201792296A 2015-06-01 2016-06-01 Электрохимические биосенсоры EA201792296A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AU2015902093A AU2015902093A0 (en) 2015-06-01 Electrochemical biosensor
PCT/AU2016/050436 WO2016191812A1 (en) 2015-06-01 2016-06-01 Electrochemical biosensor

Publications (1)

Publication Number Publication Date
EA201792296A1 true EA201792296A1 (ru) 2018-06-29

Family

ID=57439739

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201792296A EA201792296A1 (ru) 2015-06-01 2016-06-01 Электрохимические биосенсоры

Country Status (13)

Country Link
US (1) US20180245121A1 (ru)
EP (1) EP3303574A4 (ru)
JP (1) JP2018518168A (ru)
KR (1) KR20180023912A (ru)
CN (1) CN107849544A (ru)
AU (1) AU2016269840A1 (ru)
BR (1) BR112017025785A2 (ru)
CA (1) CA2986446A1 (ru)
EA (1) EA201792296A1 (ru)
IL (1) IL255834A (ru)
MX (1) MX2017015094A (ru)
WO (1) WO2016191812A1 (ru)
ZA (1) ZA201707827B (ru)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018154579A (ja) * 2017-03-16 2018-10-04 栄研化学株式会社 抗体フラグメントとグルコース脱水素酵素との融合タンパク質
WO2019207356A1 (en) * 2018-04-24 2019-10-31 The University Of Queensland Next-generation electrochemical biosensors
US11293921B2 (en) * 2018-10-03 2022-04-05 Arkray, Inc. Direct electron transfer-type oxidoreductase-modified molecular recognition element
CN113912694B (zh) * 2019-07-24 2023-05-30 暨南大学 靶向cd133的结合蛋白和应用
KR102368697B1 (ko) * 2019-10-23 2022-02-28 울산과학기술원 감광성 수지 조성물, 이를 이용하여 형성된 화소 구획 층, 및 상기 화소 구획 층을 포함하는 전자 소자
CA3160038A1 (en) * 2019-12-02 2021-06-10 Dale Hunt Methods of screening compositions for cannabinoids
GB202200051D0 (en) * 2022-01-05 2022-02-16 Univ Queensland Technology And-gate protein-based switches
DE102022000897A1 (de) 2022-03-15 2023-09-21 Ruhr-Universität Bochum, Körperschaft des öffentlichen Rechts Implantierbarer Biosensor

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000312588A (ja) * 1999-04-30 2000-11-14 Koji Hayade グルコース脱水素酵素
JP3650815B2 (ja) * 2000-11-22 2005-05-25 大学共同利用機関法人自然科学研究機構 緑色蛍光蛋白質の蛍光特性を制御することが可能なバイオセンサー蛋白質の作成方法、および前記方法により作成されるバイオセンサー蛋白質
US8118991B2 (en) * 2001-09-04 2012-02-21 Stephen Eliot Zweig Apoenzyme reactivation electrochemical detection method and assay
JP5020832B2 (ja) * 2005-03-04 2012-09-05 バイエル・ヘルスケア・エルエルシー 電気化学的バイオセンサでの酵素活性の安定化
WO2012169512A1 (ja) * 2011-06-07 2012-12-13 キッコーマン株式会社 フラビン結合型グルコースデヒドロゲナーゼ、フラビン結合型グルコースデヒドロゲナーゼの製造方法、およびそれを用いたグルコース測定方法
CN102559624B (zh) * 2012-03-05 2013-10-16 浙江德清汇宁生物科技有限公司 一种吡咯喹啉醌依赖型葡萄糖脱氢酶的热稳定性突变体及其高通量筛选方法
US9791436B2 (en) * 2012-09-12 2017-10-17 The University Of Queensland Protease-based biosensor
EP3044322A4 (en) * 2013-09-12 2017-09-06 The University Of Queensland Bimolecular protease-based biosensor
WO2016065415A1 (en) * 2014-10-27 2016-05-06 The University Of Queensland Bimolecular autoinhibited biosensor

Also Published As

Publication number Publication date
AU2016269840A1 (en) 2017-11-30
MX2017015094A (es) 2018-08-14
WO2016191812A1 (en) 2016-12-08
CA2986446A1 (en) 2016-12-08
ZA201707827B (en) 2019-11-27
CN107849544A (zh) 2018-03-27
US20180245121A1 (en) 2018-08-30
BR112017025785A2 (pt) 2018-08-07
JP2018518168A (ja) 2018-07-12
EP3303574A1 (en) 2018-04-11
IL255834A (en) 2018-01-31
EP3303574A4 (en) 2018-11-21
KR20180023912A (ko) 2018-03-07

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