ES2498790B2 - Procedimiento para la detección magneto-electroquímica sin lavados de un analito en una muestra - Google Patents

Procedimiento para la detección magneto-electroquímica sin lavados de un analito en una muestra Download PDF

Info

Publication number
ES2498790B2
ES2498790B2 ES201330398A ES201330398A ES2498790B2 ES 2498790 B2 ES2498790 B2 ES 2498790B2 ES 201330398 A ES201330398 A ES 201330398A ES 201330398 A ES201330398 A ES 201330398A ES 2498790 B2 ES2498790 B2 ES 2498790B2
Authority
ES
Spain
Prior art keywords
analyte
sample
electrochemical
compound
magneto
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES201330398A
Other languages
English (en)
Other versions
ES2498790A1 (es
Inventor
David HERNÁNDEZ SANTOS
Pablo Fanjul Bolado
Mónica ORDAX IBÁÑEZ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DROPSENS S L
DROPSENS SL
Original Assignee
DROPSENS S L
DROPSENS SL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DROPSENS S L, DROPSENS SL filed Critical DROPSENS S L
Priority to ES201330398A priority Critical patent/ES2498790B2/es
Priority to PCT/EP2014/055367 priority patent/WO2014147049A1/en
Publication of ES2498790A1 publication Critical patent/ES2498790A1/es
Application granted granted Critical
Publication of ES2498790B2 publication Critical patent/ES2498790B2/es
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Magnetic Means (AREA)

Abstract

La invención describe un procedimiento rápido, simple y eficaz para la detección magneto-electroquímica de un analito en una muestra eliminando todo paso de lavado, que comprende la incubación de dicha muestra en presencia de partículas magnéticas y de un elemento de reconocimiento ligado a un enzima en que dicho elemento de reconocimiento y dichas partículas magnéticas son capaces de interaccionar independientemente con dicho analito, la aplicación del electrodo al resultado de esta etapa de incubación en presencia de al menos un imán, la adición de un sustrato electroquímico de dicho enzima para obtener un compuesto con capacidad óxido-reductora, la medición electroquímica en la superficie del electrodo de dicho compuesto con capacidad óxido-reductora o de al menos un producto de una reacción redox generada por dicho compuesto en presencia de los componentes de la muestra, los reactivos añadidos en la incubación y del sustrato electroquímico, y la determinación de la presencia del analito evaluando el resultado de la etapa anterior respecto de una referencia.
ES201330398A 2013-03-20 2013-03-20 Procedimiento para la detección magneto-electroquímica sin lavados de un analito en una muestra Expired - Fee Related ES2498790B2 (es)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ES201330398A ES2498790B2 (es) 2013-03-20 2013-03-20 Procedimiento para la detección magneto-electroquímica sin lavados de un analito en una muestra
PCT/EP2014/055367 WO2014147049A1 (en) 2013-03-20 2014-03-18 No-wash magneto-electrochemical procedure to detect an analyte in a sample

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201330398A ES2498790B2 (es) 2013-03-20 2013-03-20 Procedimiento para la detección magneto-electroquímica sin lavados de un analito en una muestra

Publications (2)

Publication Number Publication Date
ES2498790A1 ES2498790A1 (es) 2014-09-25
ES2498790B2 true ES2498790B2 (es) 2015-06-25

Family

ID=50382431

Family Applications (1)

Application Number Title Priority Date Filing Date
ES201330398A Expired - Fee Related ES2498790B2 (es) 2013-03-20 2013-03-20 Procedimiento para la detección magneto-electroquímica sin lavados de un analito en una muestra

Country Status (2)

Country Link
ES (1) ES2498790B2 (es)
WO (1) WO2014147049A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3067116A1 (en) 2015-03-13 2016-09-14 Fundació Hospital Universitari Vall d' Hebron - Institut de Recerca Device for electrochemical detection with magnetic particles
WO2016191603A1 (en) * 2015-05-26 2016-12-01 OncoGenesis Inc. System, method and kit for detection of analytes by production of electrochemical species
CN109415752B (zh) 2016-01-27 2022-12-27 通用医疗公司 磁性电化学传感
ES2794173B2 (es) * 2019-05-17 2022-07-21 Univ Madrid Complutense Método y plataforma electroquímica inmunosensora para la detección y/o cuantificación de adulteraciones en leche y productos lácteos

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4440487A1 (de) * 1994-11-12 1996-05-15 Behringwerke Ag Heterogener Immunoassay mittels präzipitierbarer Festphase
ES2307430B1 (es) * 2007-05-09 2009-10-20 Consejo Superior De Investigaciones Cientificas Biosensor y sus aplicaciones.
AU2012202981B2 (en) * 2009-09-30 2014-01-23 Cilag Gmbh International Adhesive Composition for Use in an Immunosensor
EP2601307A4 (en) * 2010-08-06 2014-01-01 Capitalbio Corp MICROARRAY TEST WITH PARTICLES FOR THE ANALYSIS OF MOLECULAR INTERACTIONS
GB201218555D0 (en) * 2012-10-16 2012-11-28 Mode Diagnostics Ltd Immunoassay using electrochemical detection

Also Published As

Publication number Publication date
ES2498790A1 (es) 2014-09-25
WO2014147049A1 (en) 2014-09-25

Similar Documents

Publication Publication Date Title
EP4282533A3 (en) Analytical systems and methods for nucleic acid amplification using sample assigning parameters
MY170164A (en) Aptamer-based multiplexed assays
EP4249605A3 (en) Methods for analyte detection
MY192493A (en) Urinalysis device and dry reagent for quantitative urinalysis
TR201900160T4 (tr) Bir anti-ilaç antikoru varlığının veya miktarının saptanmasına yönelik analizler.
BR112014018995A2 (pt) Sistemas para executar ensaio automatizado
EA201992748A1 (ru) Диагностические анализы для обнаружения, количественного определения и/или отслеживания микроорганизмов и других аналитов
AR098473A1 (es) Método para analizar una muestra que comprende por lo menos un primer y un segundo inhibidores de óxido
NZ629703A (en) Methods and compositions for detecting multiple analytes with a single signal
EP4220158A3 (en) Urine specimen analysis device and urine specimen analysis method
MX2015013596A (es) Metodos, dispositivos y sistemas para analisis de muestras.
ES2498790B2 (es) Procedimiento para la detección magneto-electroquímica sin lavados de un analito en una muestra
BR112016024730A2 (pt) dispositivo, método e conjunto de análise imunocromatográfica
WO2016209773A9 (en) Improved assays and methods for allergen detection
GB2542576A (en) Method and kit of detecting the absence of micro-oranisms
EA201500377A1 (ru) Способ обнаружения анализируемого вещества и определение его концентрации с использованием намагничиваемых гранул
WO2014151290A3 (en) Assay for predictive biomarkers
TR201906242T4 (tr) Elektroki̇myasal deteksi̇yon metodu
BR112017001500A2 (pt) composições e métodos para a detecção e análise de tuberculose por micobactéria
BR112019007121A2 (pt) cartucho, sistema de análise e método para testagem de uma amostra
SG10201907690TA (en) Device and method for detecting target molecules
WO2015054287A3 (en) Methods and systems for electrochemically detecting or quantifying an analyte
WO2015038987A3 (en) System and method for whole-animal high-throughput compound screening
GB2547600A (en) Devices and methods for detecting norovirus on surfaces
WO2013179279A3 (en) Method and device for analyzing biological samples

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 2498790

Country of ref document: ES

Kind code of ref document: B2

Effective date: 20150625

FD2A Announcement of lapse in spain

Effective date: 20210929