ES2498790A1 - Procedimiento para la detección magneto-electroquímica sin lavados de un analito en una muestra - Google Patents

Procedimiento para la detección magneto-electroquímica sin lavados de un analito en una muestra Download PDF

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Publication number
ES2498790A1
ES2498790A1 ES201330398A ES201330398A ES2498790A1 ES 2498790 A1 ES2498790 A1 ES 2498790A1 ES 201330398 A ES201330398 A ES 201330398A ES 201330398 A ES201330398 A ES 201330398A ES 2498790 A1 ES2498790 A1 ES 2498790A1
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ES
Spain
Prior art keywords
analyte
sample
electrochemical
magneto
incubation
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Granted
Application number
ES201330398A
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English (en)
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ES2498790B2 (es
Inventor
David HERNÁNDEZ SANTOS
Pablo Fanjul Bolado
Mónica ORDAX IBÁÑEZ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DROPSENS S L
DROPSENS SL
Original Assignee
DROPSENS S L
DROPSENS SL
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Publication date
Application filed by DROPSENS S L, DROPSENS SL filed Critical DROPSENS S L
Priority to ES201330398A priority Critical patent/ES2498790B2/es
Priority to PCT/EP2014/055367 priority patent/WO2014147049A1/en
Publication of ES2498790A1 publication Critical patent/ES2498790A1/es
Application granted granted Critical
Publication of ES2498790B2 publication Critical patent/ES2498790B2/es
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Magnetic Means (AREA)

Abstract

La invención describe un procedimiento rápido, simple y eficaz para la detección magneto-electroquímica de un analito en una muestra eliminando todo paso de lavado, que comprende la incubación de dicha muestra en presencia de partículas magnéticas y de un elemento de reconocimiento ligado a un enzima en que dicho elemento de reconocimiento y dichas partículas magnéticas son capaces de interaccionar independientemente con dicho analito, la aplicación del electrodo al resultado de esta etapa de incubación en presencia de al menos un imán, la adición de un sustrato electroquímico de dicho enzima para obtener un compuesto con capacidad óxido-reductora, la medición electroquímica en la superficie del electrodo de dicho compuesto con capacidad óxido-reductora o de al menos un producto de una reacción redox generada por dicho compuesto en presencia de los componentes de la muestra, los reactivos añadidos en la incubación y del sustrato electroquímico, y la determinación de la presencia del analito evaluando el resultado de la etapa anterior respecto de una referencia.

Description

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Claims (1)

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ES201330398A 2013-03-20 2013-03-20 Procedimiento para la detección magneto-electroquímica sin lavados de un analito en una muestra Expired - Fee Related ES2498790B2 (es)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ES201330398A ES2498790B2 (es) 2013-03-20 2013-03-20 Procedimiento para la detección magneto-electroquímica sin lavados de un analito en una muestra
PCT/EP2014/055367 WO2014147049A1 (en) 2013-03-20 2014-03-18 No-wash magneto-electrochemical procedure to detect an analyte in a sample

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201330398A ES2498790B2 (es) 2013-03-20 2013-03-20 Procedimiento para la detección magneto-electroquímica sin lavados de un analito en una muestra

Publications (2)

Publication Number Publication Date
ES2498790A1 true ES2498790A1 (es) 2014-09-25
ES2498790B2 ES2498790B2 (es) 2015-06-25

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
ES201330398A Expired - Fee Related ES2498790B2 (es) 2013-03-20 2013-03-20 Procedimiento para la detección magneto-electroquímica sin lavados de un analito en una muestra

Country Status (2)

Country Link
ES (1) ES2498790B2 (es)
WO (1) WO2014147049A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3067116A1 (en) 2015-03-13 2016-09-14 Fundació Hospital Universitari Vall d' Hebron - Institut de Recerca Device for electrochemical detection with magnetic particles
WO2016191603A1 (en) * 2015-05-26 2016-12-01 OncoGenesis Inc. System, method and kit for detection of analytes by production of electrochemical species
WO2017132564A2 (en) * 2016-01-27 2017-08-03 The General Hospital Corporation Magnetic electrochemical sensing
ES2794173B2 (es) * 2019-05-17 2022-07-21 Univ Madrid Complutense Método y plataforma electroquímica inmunosensora para la detección y/o cuantificación de adulteraciones en leche y productos lácteos

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2172547T3 (es) * 1994-11-12 2002-10-01 Dade Behring Marburg Gmbh Inmunoensayo heterogeneo mediante fase solida precipitable.
EP2148191A1 (en) * 2007-05-09 2010-01-27 Consejo Superior De Investigaciones Científicas Impedimetric sensor and applications thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2012202981B2 (en) * 2009-09-30 2014-01-23 Cilag Gmbh International Adhesive Composition for Use in an Immunosensor
WO2012016357A1 (en) * 2010-08-06 2012-02-09 Capitalbio Corporation Microarray-based assay integrated with particles for analyzing molecular interactions
GB201218555D0 (en) * 2012-10-16 2012-11-28 Mode Diagnostics Ltd Immunoassay using electrochemical detection

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2172547T3 (es) * 1994-11-12 2002-10-01 Dade Behring Marburg Gmbh Inmunoensayo heterogeneo mediante fase solida precipitable.
EP2148191A1 (en) * 2007-05-09 2010-01-27 Consejo Superior De Investigaciones Científicas Impedimetric sensor and applications thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
AFONSO A S et al. Electrochemical detection of Salmonella using gold nanoparticles.Biosensors and Bioelectronics 20130215 Elsevier Ltd gbr 15/02/2013 VOL: 40 No: 1 Pags: 121 - 126 ISSN 0956-5663 (print) ISSN 1873-4235 (electronic) Doi: doi:10.1016/j.bios.2012.06.054 *
LAUREYN W et al. Nanoscaled interdigitated titanium electrodes for impedimetric biosensing.Sensors and Actuators B: Chemical: international journal devoted to research and development of physical and chemical transducers, 20000825 Elsevier S.A, CH 25/08/2000 VOL: 68 No: 1-3 Pags: 360 - 370 ISSN 0925-4005 Doi: doi:10.1016/S0925-4005(00)00489-5 *
MORENO-GUZMAN MARIA et al. A disposable electrochemical immunosensor for prolactin involving affinity reaction on streptavidin-functionalized magnetic particles.Analytica Chimica Acta APR 29 2011 00/04/2011 VOL: 692 No: 1-2 Pags: 125-130 ISSN 0003-2670(print) ISSN 1873-4324(electronic) Doi: doi:10.1016/j.aca.2011.02.062MORENO-GUZMAN MARIA et al. A disposable electrochemical immunosensor for prolactin involving affinity reaction on streptavidin-functionalized magnetic particles.Analytica Chimica Acta APR 29 2011 00/04/2011 VOL: 692 No: 1-2 Pags: 125-130 ISSN 0003-2670(print) ISSN 1873-4324(electronic) Doi: doi:10.1016/j.aca.2011.02.062 *

Also Published As

Publication number Publication date
ES2498790B2 (es) 2015-06-25
WO2014147049A1 (en) 2014-09-25

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