ES2498790A1 - Procedimiento para la detección magneto-electroquímica sin lavados de un analito en una muestra - Google Patents
Procedimiento para la detección magneto-electroquímica sin lavados de un analito en una muestra Download PDFInfo
- Publication number
- ES2498790A1 ES2498790A1 ES201330398A ES201330398A ES2498790A1 ES 2498790 A1 ES2498790 A1 ES 2498790A1 ES 201330398 A ES201330398 A ES 201330398A ES 201330398 A ES201330398 A ES 201330398A ES 2498790 A1 ES2498790 A1 ES 2498790A1
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- ES
- Spain
- Prior art keywords
- analyte
- sample
- electrochemical
- magneto
- incubation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
- G01N33/5438—Electrodes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Materials By The Use Of Magnetic Means (AREA)
Abstract
La invención describe un procedimiento rápido, simple y eficaz para la detección magneto-electroquímica de un analito en una muestra eliminando todo paso de lavado, que comprende la incubación de dicha muestra en presencia de partículas magnéticas y de un elemento de reconocimiento ligado a un enzima en que dicho elemento de reconocimiento y dichas partículas magnéticas son capaces de interaccionar independientemente con dicho analito, la aplicación del electrodo al resultado de esta etapa de incubación en presencia de al menos un imán, la adición de un sustrato electroquímico de dicho enzima para obtener un compuesto con capacidad óxido-reductora, la medición electroquímica en la superficie del electrodo de dicho compuesto con capacidad óxido-reductora o de al menos un producto de una reacción redox generada por dicho compuesto en presencia de los componentes de la muestra, los reactivos añadidos en la incubación y del sustrato electroquímico, y la determinación de la presencia del analito evaluando el resultado de la etapa anterior respecto de una referencia.
Description
Claims (1)
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imagen1 imagen2 imagen3 imagen4
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES201330398A ES2498790B2 (es) | 2013-03-20 | 2013-03-20 | Procedimiento para la detección magneto-electroquímica sin lavados de un analito en una muestra |
PCT/EP2014/055367 WO2014147049A1 (en) | 2013-03-20 | 2014-03-18 | No-wash magneto-electrochemical procedure to detect an analyte in a sample |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES201330398A ES2498790B2 (es) | 2013-03-20 | 2013-03-20 | Procedimiento para la detección magneto-electroquímica sin lavados de un analito en una muestra |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2498790A1 true ES2498790A1 (es) | 2014-09-25 |
ES2498790B2 ES2498790B2 (es) | 2015-06-25 |
Family
ID=50382431
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES201330398A Expired - Fee Related ES2498790B2 (es) | 2013-03-20 | 2013-03-20 | Procedimiento para la detección magneto-electroquímica sin lavados de un analito en una muestra |
Country Status (2)
Country | Link |
---|---|
ES (1) | ES2498790B2 (es) |
WO (1) | WO2014147049A1 (es) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3067116A1 (en) | 2015-03-13 | 2016-09-14 | Fundació Hospital Universitari Vall d' Hebron - Institut de Recerca | Device for electrochemical detection with magnetic particles |
US10670595B2 (en) * | 2015-05-26 | 2020-06-02 | Oncogenesis, Inc. | System, method and kit for detection of analytes by production of electrochemical species |
WO2017132564A2 (en) * | 2016-01-27 | 2017-08-03 | The General Hospital Corporation | Magnetic electrochemical sensing |
ES2794173B2 (es) * | 2019-05-17 | 2022-07-21 | Univ Madrid Complutense | Método y plataforma electroquímica inmunosensora para la detección y/o cuantificación de adulteraciones en leche y productos lácteos |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2172547T3 (es) * | 1994-11-12 | 2002-10-01 | Dade Behring Marburg Gmbh | Inmunoensayo heterogeneo mediante fase solida precipitable. |
EP2148191A1 (en) * | 2007-05-09 | 2010-01-27 | Consejo Superior De Investigaciones Científicas | Impedimetric sensor and applications thereof |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2012202981B2 (en) * | 2009-09-30 | 2014-01-23 | Cilag Gmbh International | Adhesive Composition for Use in an Immunosensor |
US9803236B2 (en) * | 2010-08-06 | 2017-10-31 | Tsinghua University | Microarray-based assay integrated with particles for analyzing molecular interactions |
GB201218555D0 (en) * | 2012-10-16 | 2012-11-28 | Mode Diagnostics Ltd | Immunoassay using electrochemical detection |
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2013
- 2013-03-20 ES ES201330398A patent/ES2498790B2/es not_active Expired - Fee Related
-
2014
- 2014-03-18 WO PCT/EP2014/055367 patent/WO2014147049A1/en active Application Filing
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2172547T3 (es) * | 1994-11-12 | 2002-10-01 | Dade Behring Marburg Gmbh | Inmunoensayo heterogeneo mediante fase solida precipitable. |
EP2148191A1 (en) * | 2007-05-09 | 2010-01-27 | Consejo Superior De Investigaciones Científicas | Impedimetric sensor and applications thereof |
Non-Patent Citations (3)
Title |
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AFONSO A S et al. Electrochemical detection of Salmonella using gold nanoparticles.Biosensors and Bioelectronics 20130215 Elsevier Ltd gbr 15/02/2013 VOL: 40 No: 1 Pags: 121 - 126 ISSN 0956-5663 (print) ISSN 1873-4235 (electronic) Doi: doi:10.1016/j.bios.2012.06.054 * |
LAUREYN W et al. Nanoscaled interdigitated titanium electrodes for impedimetric biosensing.Sensors and Actuators B: Chemical: international journal devoted to research and development of physical and chemical transducers, 20000825 Elsevier S.A, CH 25/08/2000 VOL: 68 No: 1-3 Pags: 360 - 370 ISSN 0925-4005 Doi: doi:10.1016/S0925-4005(00)00489-5 * |
MORENO-GUZMAN MARIA et al. A disposable electrochemical immunosensor for prolactin involving affinity reaction on streptavidin-functionalized magnetic particles.Analytica Chimica Acta APR 29 2011 00/04/2011 VOL: 692 No: 1-2 Pags: 125-130 ISSN 0003-2670(print) ISSN 1873-4324(electronic) Doi: doi:10.1016/j.aca.2011.02.062MORENO-GUZMAN MARIA et al. A disposable electrochemical immunosensor for prolactin involving affinity reaction on streptavidin-functionalized magnetic particles.Analytica Chimica Acta APR 29 2011 00/04/2011 VOL: 692 No: 1-2 Pags: 125-130 ISSN 0003-2670(print) ISSN 1873-4324(electronic) Doi: doi:10.1016/j.aca.2011.02.062 * |
Also Published As
Publication number | Publication date |
---|---|
WO2014147049A1 (en) | 2014-09-25 |
ES2498790B2 (es) | 2015-06-25 |
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