TR201820335T4 - Katı Faz Cihazında Nükleik Asitlerin İzolasyonu Ve Analizi İçin Etiketsiz Yöntemler. - Google Patents

Katı Faz Cihazında Nükleik Asitlerin İzolasyonu Ve Analizi İçin Etiketsiz Yöntemler. Download PDF

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Publication number
TR201820335T4
TR201820335T4 TR2018/20335T TR201820335T TR201820335T4 TR 201820335 T4 TR201820335 T4 TR 201820335T4 TR 2018/20335 T TR2018/20335 T TR 2018/20335T TR 201820335 T TR201820335 T TR 201820335T TR 201820335 T4 TR201820335 T4 TR 201820335T4
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TR
Turkey
Prior art keywords
solid phase
isolation
analysis
nucleic acid
nucleic acids
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Application number
TR2018/20335T
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English (en)
Inventor
Shin Yong
Kyoung Park Mi
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One Biomed Pte Ltd
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Publication date
Application filed by One Biomed Pte Ltd filed Critical One Biomed Pte Ltd
Publication of TR201820335T4 publication Critical patent/TR201820335T4/tr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Dispersion Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Buluş bir katı faz cihazında nükleik asit moleküllerinin izolasyonu ve/veya analizi için bir yöntem ile ilgili olup, (i) bir nükleik asit numunesinin Dimetil adipimidat (DMA) ile, nükleik asit kompleksinin DMA ile oluşumuna olanak veren koşullar altında inkübe edilmesini; (ii) (i) kompleksinin katı faz cihazının aminlenmiş bir yüzeyi ile temas ettirilmesini; ve (iii) yüksek bir pH elüsyon çözeltisi kullanılarak DMA'lı nükleik asit kompleksinin yüzeyden izole edilmesini ve/veya kompleksin nükleik asidinin analiz edilmesini içerir.
TR2018/20335T 2012-12-13 2013-12-13 Katı Faz Cihazında Nükleik Asitlerin İzolasyonu Ve Analizi İçin Etiketsiz Yöntemler. TR201820335T4 (tr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SG2012091732 2012-12-13

Publications (1)

Publication Number Publication Date
TR201820335T4 true TR201820335T4 (tr) 2019-01-21

Family

ID=54367294

Family Applications (1)

Application Number Title Priority Date Filing Date
TR2018/20335T TR201820335T4 (tr) 2012-12-13 2013-12-13 Katı Faz Cihazında Nükleik Asitlerin İzolasyonu Ve Analizi İçin Etiketsiz Yöntemler.

Country Status (9)

Country Link
US (2) US9856520B2 (tr)
EP (1) EP2931921B8 (tr)
JP (1) JP6455438B2 (tr)
KR (1) KR102091041B1 (tr)
CN (1) CN104968803B (tr)
ES (1) ES2706761T3 (tr)
SG (1) SG11201504388XA (tr)
TR (1) TR201820335T4 (tr)
WO (1) WO2014092653A1 (tr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2906928A4 (en) 2012-10-15 2016-11-09 Nanocellect Biomedical Inc SYSTEMS, APPARATUS AND METHODS FOR SORTING PARTICLES
KR101913208B1 (ko) 2016-05-17 2018-10-30 울산대학교 산학협력단 고형상 대상물을 이용한 핵산 추출방법
WO2017200249A1 (ko) * 2016-05-17 2017-11-23 울산대학교 산학협력단 고형상 대상물을 이용한 핵산 추출방법
WO2018038549A1 (ko) * 2016-08-24 2018-03-01 울산대학교 산학협력단 Dtbp를 이용한 미생물 농축 또는 핵산 추출 방법
KR102026683B1 (ko) * 2016-08-24 2019-09-30 울산대학교 산학협력단 Dtbp를 이용한 미생물 농축 또는 핵산 추출 방법
WO2018169307A1 (ko) * 2017-03-15 2018-09-20 울산대학교 산학협력단 규조토를 이용한 미생물 농축 방법 및 핵산 추출 방법
KR102136694B1 (ko) * 2017-03-15 2020-07-22 주식회사 인퓨전텍 규조토를 이용한 미생물 농축 방법 및 핵산 추출 방법
WO2019078638A2 (ko) * 2017-10-18 2019-04-25 울산대학교 산학협력단 동형2기능성 이미도에스터를 이용한 병원체 농축 방법
KR102136696B1 (ko) 2017-10-18 2020-07-22 주식회사 인퓨전텍 동형2기능성 이미도에스터를 이용한 병원체 농축 방법
US11603526B2 (en) 2017-10-30 2023-03-14 Infusion Tech Pathogen lysis and nucleic acid extraction method using zinc oxide nanostar
WO2019088527A2 (ko) * 2017-10-30 2019-05-09 울산대학교 산학협력단 산화아연 나노스타를 이용한 병원체 용균 및 핵산 추출 방법
WO2019088429A1 (ko) * 2017-10-30 2019-05-09 울산대학교 산학협력단 동형2기능성 이미도에스터를 이용한 액체생검 유래 생체물질의 추출 방법
KR102260012B1 (ko) * 2020-09-29 2021-06-03 주식회사 에이아이더뉴트리진 미생물 농축 또는 핵산 추출용 조성물 및 이를 이용한 미생물 농축 또는 핵산 추출 방법

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002023185A2 (en) 2000-09-15 2002-03-21 Engeneos, Inc. Devices and methods for direct electronic readout of biomolecular interactions
CA2478592A1 (en) * 2002-03-07 2003-09-18 The Johns Hopkins University School Of Medicine Genomic screen for epigenetically silenced genes associated with cancer
US7700282B2 (en) * 2004-10-11 2010-04-20 Epigenomics Ag Method for the carry-over protection in DNA amplification systems targeting methylation analysis achieved by a modified pre-treatment of nucleic acids
JP5670194B2 (ja) 2007-10-09 2015-02-18 ユニヴァーシティー オブ ノートル ダム デュ ラック 多種類の標的を検出するためのマイクロ流体プラットフォーム
KR101598867B1 (ko) * 2008-04-30 2016-03-03 산바이오 인코포레이티드 Ｄｎａ 메틸화에 변형을 가진 신경 재생 세포

Also Published As

Publication number Publication date
JP6455438B2 (ja) 2019-01-23
EP2931921B8 (en) 2019-01-09
US9856520B2 (en) 2018-01-02
ES2706761T3 (es) 2019-04-01
JP2016501028A (ja) 2016-01-18
EP2931921B1 (en) 2018-10-31
WO2014092653A1 (en) 2014-06-19
US20150322486A1 (en) 2015-11-12
SG11201504388XA (en) 2015-07-30
KR20150096444A (ko) 2015-08-24
CN104968803B (zh) 2019-07-26
KR102091041B1 (ko) 2020-03-23
US10584373B2 (en) 2020-03-10
EP2931921A1 (en) 2015-10-21
US20180073061A1 (en) 2018-03-15
EP2931921A4 (en) 2016-08-24
CN104968803A (zh) 2015-10-07

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