MX2021006899A - A?adiduras de acidos nucleicos sinteticos. - Google Patents

A?adiduras de acidos nucleicos sinteticos.

Info

Publication number
MX2021006899A
MX2021006899A MX2021006899A MX2021006899A MX2021006899A MX 2021006899 A MX2021006899 A MX 2021006899A MX 2021006899 A MX2021006899 A MX 2021006899A MX 2021006899 A MX2021006899 A MX 2021006899A MX 2021006899 A MX2021006899 A MX 2021006899A
Authority
MX
Mexico
Prior art keywords
synthetic nucleic
ins
nucleic acid
tracking
methods
Prior art date
Application number
MX2021006899A
Other languages
English (en)
Inventor
Michael Kertesz
Michael Rosen
Fred C Christians
Igor D Vilfan
Timothy A Blauwkamp
Shivkumar Venkatasubrahmanyam
Rene Sit
Original Assignee
Karius Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Karius Inc filed Critical Karius Inc
Publication of MX2021006899A publication Critical patent/MX2021006899A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10—Sequence alignment; Homology search
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2545/00—Reactions characterised by their quantitative nature
    • C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C12Q2545/101—Reactions characterised by their quantitative nature the purpose being quantitative analysis with an internal standard/control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Immunology (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Esta descripción proporciona métodos para determinar la abundancia relativa de una o más especies no hospederas en una muestra de un hospedero. También se proporcionan métodos que implican la adición de concentraciones conocidas de ácidos nucleicos sintéticos a una muestra y llevan a cabo ensayos de secuenciación para identificar especies no hospederas tal como patógenos. También se proporcionan métodos para rastrear muestras, rastrear reactivos, y rastrear pérdida de diversidad en los ensayos de secuenciación.
MX2021006899A 2016-03-25 2018-09-20 A?adiduras de acidos nucleicos sinteticos. MX2021006899A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662313668P 2016-03-25 2016-03-25
US201662397873P 2016-09-21 2016-09-21
US201762451363P 2017-01-27 2017-01-27

Publications (1)

Publication Number Publication Date
MX2021006899A true MX2021006899A (es) 2021-07-07

Family

ID=59897735

Family Applications (3)

Application Number Title Priority Date Filing Date
MX2018011429A MX2018011429A (es) 2016-03-25 2017-03-24 Añadiduras de ácidos nucleicos sintéticos.
MX2021006899A MX2021006899A (es) 2016-03-25 2018-09-20 A?adiduras de acidos nucleicos sinteticos.
MX2021006911A MX2021006911A (es) 2016-03-25 2018-09-20 A?adiduras de acidos nucleicos sinteticos.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2018011429A MX2018011429A (es) 2016-03-25 2017-03-24 Añadiduras de ácidos nucleicos sintéticos.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2021006911A MX2021006911A (es) 2016-03-25 2018-09-20 A?adiduras de acidos nucleicos sinteticos.

Country Status (11)

Country Link
US (4) US9976181B2 (es)
EP (3) EP4198146A3 (es)
JP (3) JP6743268B2 (es)
KR (3) KR102531487B1 (es)
CN (2) CN116987777A (es)
AU (4) AU2017237199B2 (es)
BR (1) BR112018069557A2 (es)
CA (2) CA3185611A1 (es)
IL (2) IL260924B (es)
MX (3) MX2018011429A (es)
WO (1) WO2017165864A1 (es)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2015364286B2 (en) 2014-12-20 2021-11-04 Arc Bio, Llc Compositions and methods for targeted depletion, enrichment, and partitioning of nucleic acids using CRISPR/Cas system proteins
US10538758B2 (en) 2015-08-19 2020-01-21 Arc Bio, Llc Capture of nucleic acids using a nucleic acid-guided nuclease-based system
CA3185611A1 (en) 2016-03-25 2017-09-28 Karius, Inc. Synthetic nucleic acid spike-ins
EP3610034B1 (en) 2017-04-12 2022-06-08 Karius, Inc. Sample preparation methods, systems and compositions
SG11202000444PA (en) 2017-08-04 2020-02-27 Billiontoone Inc Sequencing output determination and analysis with target-associated molecules in quantification associated with biological targets
US11646100B2 (en) 2017-08-04 2023-05-09 Billiontoone, Inc. Target-associated molecules for characterization associated with biological targets
US11519024B2 (en) 2017-08-04 2022-12-06 Billiontoone, Inc. Homologous genomic regions for characterization associated with biological targets
CN109680343B (zh) * 2017-10-18 2022-02-18 深圳华大生命科学研究院 一种外泌体微量dna的建库方法
JP7164125B2 (ja) * 2018-01-05 2022-11-01 ビリオントゥーワン，インコーポレイテッド シーケンシングベースのアッセイの妥当性を確保するための品質管理鋳型
EP3752636A4 (en) * 2018-02-15 2022-01-26 Thrive Earlier Detection Corp. BAR CODED MOLECULAR STANDARDS
US11970786B2 (en) * 2018-02-15 2024-04-30 Bioo Scientific Corporation Methods and kits for detecting contamination and sample misidentification
WO2019178157A1 (en) 2018-03-16 2019-09-19 Karius, Inc. Sample series to differentiate target nucleic acids from contaminant nucleic acids
GB201805676D0 (en) * 2018-04-05 2018-05-23 Imperial Innovations Ltd Compositions
WO2019226648A1 (en) 2018-05-21 2019-11-28 Battelle Memorial Institute Methods and control compositions for sequencing and chemical analyses
CN112639987A (zh) 2018-06-29 2021-04-09 格瑞尔公司 核酸重排和整合分析
DE102018213027A1 (de) * 2018-08-03 2020-02-06 Robert Bosch Gmbh Reaktionsgemisch, Verfahren und Kit zur Durchführung einer quantitativen Echtzeit-PCR
US12071651B2 (en) 2018-08-06 2024-08-27 Billiontoone, Inc. Dilution tagging for quantification of biological targets
EP4296373A3 (en) * 2018-10-04 2024-01-24 Arc Bio, LLC Normalization controls for managing low sample inputs in next generation sequencing
EP3874060B1 (en) * 2018-10-31 2024-10-16 Guardant Health, Inc. Method and system for calibrating epigenetic partitioning assays
EP3884065B1 (en) 2018-11-21 2024-08-14 Karius, Inc. Direct-to-library methods, systems, and compositions
CA3118742A1 (en) * 2018-11-21 2020-05-28 Karius, Inc. Detection and prediction of infectious disease
EP3894553A4 (en) * 2018-12-13 2022-06-29 Battelle Memorial Institute Methods and control compositions for a quantitative polymerase chain reaction
CA3119980A1 (en) * 2018-12-20 2020-06-25 Guardant Health, Inc. Methods, compositions, and systems for improving recovery of nucleic acid molecules
EP3931356A1 (en) * 2019-02-28 2022-01-05 Inivata Ltd. Method for quantifying the amount of a target sequence in a nucleic acid sample
US20220364156A1 (en) * 2019-06-28 2022-11-17 Biota Technology, Inc. Estimating a quantity of molecules in a sample
WO2021074382A1 (en) * 2019-10-16 2021-04-22 Stilla Technologies Determination of nucleic acid sequence concentrations
CN111599409B (zh) * 2020-05-20 2022-05-20 电子科技大学 基于MapReduce并行的circRNA识别方法
KR20220045451A (ko) 2020-10-05 2022-04-12 주식회사 엘지에너지솔루션 배터리 상태 진단 장치 및 방법
CN112266978A (zh) * 2020-10-22 2021-01-26 深圳国际旅行卫生保健中心(深圳海关口岸门诊部) 引物探针组合、检测试剂盒及其应用
CN115109840A (zh) * 2021-03-18 2022-09-27 厦门大学 一种分析目标核酸中突变的存在及其类型的方法
BR112023019894A2 (pt) * 2021-03-29 2023-11-14 Illumina Inc Composições e métodos para avaliar os danos ao dna em uma biblioteca e normalizar a distorção do tamanho do amplicon
US12043873B2 (en) 2022-03-21 2024-07-23 Billiontoone, Inc. Molecule counting of methylated cell-free DNA for treatment monitoring
WO2024220566A1 (en) 2023-04-18 2024-10-24 Karius, Inc. Sample processing to enrich for select species of nucleic acids

Family Cites Families (100)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
USRE39920E1 (en) 1997-05-30 2007-11-13 Xenomics, Inc. Methods for detection of nucleic acid sequences in urine
US20040106110A1 (en) 1998-07-30 2004-06-03 Solexa, Ltd. Preparation of polynucleotide arrays
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US20030022207A1 (en) 1998-10-16 2003-01-30 Solexa, Ltd. Arrayed polynucleotides and their use in genome analysis
US7211390B2 (en) 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
WO2001023610A2 (en) 1999-09-29 2001-04-05 Solexa Ltd. Polynucleotide sequencing
GB0002389D0 (en) 2000-02-02 2000-03-22 Solexa Ltd Molecular arrays
AU2001293163A1 (en) 2000-09-27 2002-04-08 Lynx Therapeutics, Inc. Method for determining relative abundance of nucleic acid sequences
US6753137B2 (en) 2001-01-31 2004-06-22 The Chinese University Of Hong Kong Circulating epstein-barr virus DNA in the serum of patients with gastric carcinoma
JP2003101204A (ja) 2001-09-25 2003-04-04 Nec Kansai Ltd 配線基板及び配線基板の製造方法並びに電子部品
US6902921B2 (en) 2001-10-30 2005-06-07 454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
US20050124022A1 (en) 2001-10-30 2005-06-09 Maithreyan Srinivasan Novel sulfurylase-luciferase fusion proteins and thermostable sulfurylase
US20050202414A1 (en) 2001-11-15 2005-09-15 The Regents Of The University Of California Apparatus and methods for detecting a microbe in a sample
WO2004069849A2 (en) 2003-01-29 2004-08-19 454 Corporation Bead emulsion nucleic acid amplification
WO2004081183A2 (en) 2003-03-07 2004-09-23 Rubicon Genomics, Inc. In vitro dna immortalization and whole genome amplification using libraries generated from randomly fragmented dna
US20070111233A1 (en) 2003-10-30 2007-05-17 Bianchi Diana W Prenatal diagnosis using cell-free fetal DNA in amniotic fluid
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US20060024711A1 (en) 2004-07-02 2006-02-02 Helicos Biosciences Corporation Methods for nucleic acid amplification and sequence determination
US7276720B2 (en) 2004-07-19 2007-10-02 Helicos Biosciences Corporation Apparatus and methods for analyzing samples
US20060012793A1 (en) 2004-07-19 2006-01-19 Helicos Biosciences Corporation Apparatus and methods for analyzing samples
US20060024678A1 (en) 2004-07-28 2006-02-02 Helicos Biosciences Corporation Use of single-stranded nucleic acid binding proteins in sequencing
EP1647600A3 (en) 2004-09-17 2006-06-28 Affymetrix, Inc. (A US Entity) Methods for identifying biological samples by addition of nucleic acid bar-code tags
US7914982B2 (en) 2005-02-17 2011-03-29 Trovagene, Inc. Methods for detecting pathogen specific nucleic acids in urine
EP1856295B1 (en) 2005-02-17 2013-04-03 TrovaGene, Inc. Compositions and methods for detecting viral specific nucleic acids in urine
ITRM20050067A1 (it) 2005-02-17 2006-08-18 Istituto Naz Per Le Malattie I Metodo per la rivelazione di acidi nucleici virali o di origine virale nelle urine.
EP1885877A2 (en) 2005-02-17 2008-02-13 Istituto Nazionale per le Malattie Infettive "Lazzaro Spallanzani" IRCCS Compositions and methods for detecting pathogen specific nucleic acids in urine
ITRM20050068A1 (it) 2005-02-17 2006-08-18 Istituto Naz Per Le Malattie I Metodo per la rivelazione di acidi nucleici di agenti patogeni batterici o di parassiti nelle urine.
US8532930B2 (en) 2005-11-26 2013-09-10 Natera, Inc. Method for determining the number of copies of a chromosome in the genome of a target individual using genetic data from genetically related individuals
US8101353B2 (en) 2007-12-18 2012-01-24 Advanced Analytical Technologies, Inc. System and method for nucleotide sequence profiling for sample identification
WO2009099602A1 (en) * 2008-02-04 2009-08-13 Massachusetts Institute Of Technology Selection of nucleic acids by solution hybridization to oligonucleotide baits
US20110160290A1 (en) * 2008-05-21 2011-06-30 Muneesh Tewari Use of extracellular rna to measure disease
ES2595489T3 (es) 2008-07-18 2016-12-30 Trovagene, Inc. Métodos para la detección de secuencias de ácidos nucleicos "ultracortos" basados en PCR
US20100062494A1 (en) * 2008-08-08 2010-03-11 President And Fellows Of Harvard College Enzymatic oligonucleotide pre-adenylation
EP3378951B1 (en) 2008-09-20 2020-05-13 The Board of Trustees of the Leland Stanford Junior University Noninvasive diagnosis of aneuploidy by sequencing
WO2011015944A2 (en) 2009-08-06 2011-02-10 Cedars-Sinai Medical Center Use of free dna as an early predictor of severity in acute pancreatitis
GB2488289A (en) 2009-11-06 2012-08-22 Univ Leland Stanford Junior Non-invasive diagnosis of graft rejection in organ transplant patients
US8835358B2 (en) * 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
SG186987A1 (en) 2010-06-11 2013-02-28 Pathogenica Inc Nucleic acids for multiplex organism detection and methods of use and making the same
WO2012012703A2 (en) 2010-07-23 2012-01-26 Esoterix Genetic Laboratories, Llc Identification of differentially represented fetal or maternal genomic regions and uses thereof
US20120077185A1 (en) 2010-08-06 2012-03-29 Tandem Diagnostics, Inc. Detection of genetic abnormalities and infectious disease
EP3115468B1 (en) * 2010-09-21 2018-07-25 Agilent Technologies, Inc. Increasing confidence of allele calls with molecular counting
CN103620055A (zh) 2010-12-07 2014-03-05 利兰·斯坦福青年大学托管委员会 在全基因组规模非侵入性确定亲本单倍型的胎儿遗传
WO2012129363A2 (en) * 2011-03-24 2012-09-27 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
CN103814139B (zh) 2011-04-01 2018-11-13 澳康姆生物实验室公司 用于检测无细胞的病原体特异性核酸的方法和试剂盒
US9809904B2 (en) * 2011-04-21 2017-11-07 University Of Washington Through Its Center For Commercialization Methods for retrieval of sequence-verified DNA constructs
US20140179726A1 (en) 2011-05-19 2014-06-26 Virginia Commonwealth University Gut microflora as biomarkers for the prognosis of cirrhosis and brain dysfunction
CN103890245B (zh) * 2011-05-20 2020-11-17 富鲁达公司 核酸编码反应
US20140249764A1 (en) 2011-06-06 2014-09-04 Koninklijke Philips N.V. Method for Assembly of Nucleic Acid Sequence Data
US9194006B2 (en) 2011-07-06 2015-11-24 Quest Diagnostics Investments Incorporated Direct amplification and detection of viral and bacterial pathogens
US20130024127A1 (en) 2011-07-19 2013-01-24 John Stuelpnagel Determination of source contributions using binomial probability calculations
EP2758538B1 (en) 2011-09-22 2018-10-31 Lineage Biosciences, Inc. Methods for analyzing heterogeneous samples
KR101272017B1 (ko) * 2011-09-23 2013-06-07 주식회사 랩 지노믹스 비뇨생식기 감염 질환 진단용 ｄｎａ칩
EP2764458B1 (en) 2011-10-06 2021-04-07 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
KR20140087044A (ko) * 2011-11-01 2014-07-08 패소제니카 아이엔씨. 유기체 검출을 위한 방법 및 시스템
PL2805280T3 (pl) 2012-01-20 2022-11-21 Sequenom, Inc. Procesy diagnostyczne będące czynnikiem warunków doświadczalnych
JP6320302B2 (ja) 2012-01-27 2018-05-09 ザ ボード オブ トラスティーズ オブ ザ リーランド スタンフォード ジュニア ユニバーシティ 無細胞ｒｎａをプロファイリングおよび定量するための方法
PT2814959T (pt) * 2012-02-17 2018-04-12 Hutchinson Fred Cancer Res Composições e métodos para a identificação exata de mutações
US9892230B2 (en) 2012-03-08 2018-02-13 The Chinese University Of Hong Kong Size-based analysis of fetal or tumor DNA fraction in plasma
CA2866324C (en) 2012-03-13 2019-01-15 The Chinese University Of Hong Kong Methods for analyzing massively parallel sequencing data for noninvasive prenatal diagnosis
CA3209140A1 (en) 2012-04-19 2013-10-24 The Medical College Of Wisconsin, Inc. Highly sensitive surveillance using detection of cell free dna
EP2653562A1 (en) 2012-04-20 2013-10-23 Institut Pasteur Anellovirus genome quantification as a biomarker of immune suppression
EA201590027A1 (ru) 2012-06-15 2015-05-29 Гарри Стилли Способы детекции заболеваний или состояний
KR102028375B1 (ko) 2012-09-04 2019-10-04 가던트 헬쓰, 인크. 희귀 돌연변이 및 카피수 변이를 검출하기 위한 시스템 및 방법
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US20140066317A1 (en) 2012-09-04 2014-03-06 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
EP3026124A1 (en) 2012-10-31 2016-06-01 Genesupport SA Non-invasive method for detecting a fetal chromosomal aneuploidy
JP6009096B2 (ja) 2012-11-26 2016-10-19 ザ・ユニバーシティ・オブ・トレド 核酸の標準化された配列決定のための方法およびその使用
WO2014127484A1 (en) * 2013-02-21 2014-08-28 British Columbia Cancer Agency Branch Spike-in control nucleic acids for sample tracking
US20140242582A1 (en) 2013-02-28 2014-08-28 Ariosa Diagnostics, Inc. Detection of genetic abnormalities using ligation-based detection and digital pcr
GB2528205B (en) 2013-03-15 2020-06-03 Guardant Health Inc Systems and methods to detect rare mutations and copy number variation
CA3156663A1 (en) 2013-03-15 2014-09-18 Verinata Health, Inc. Generating cell-free dna libraries directly from blood
WO2014165596A1 (en) 2013-04-03 2014-10-09 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9422551B2 (en) * 2013-05-29 2016-08-23 New England Biolabs, Inc. Adapters for ligation to RNA in an RNA library with reduced bias
WO2015069374A1 (en) * 2013-11-07 2015-05-14 Agilent Technologies, Inc. Plurality of transposase adapters for dna manipulations
CN105829589B (zh) 2013-11-07 2021-02-02 小利兰·斯坦福大学理事会 用于分析人体微生物组及其组分的无细胞核酸
AU2014346399A1 (en) * 2013-11-11 2016-06-02 Arizona Board Of Regents On Behalf Of Northern Arizona University Systems and methods for universal tail-based indexing strategies for amplicon sequencing
EP3068896B1 (en) * 2013-11-12 2018-08-08 Life Technologies Corporation Reagents and methods for sequencing
WO2015085105A1 (en) 2013-12-04 2015-06-11 University Of Alaska Fairbanks Methods and compositions for enriching non-host sequences in host samples
KR102640585B1 (ko) 2013-12-11 2024-02-23 아큐라젠 홀딩스 리미티드 희귀 서열 변이를 검출하기 위한 조성물 및 방법
CN106103713B (zh) 2014-02-03 2021-05-28 赛默飞世尔科技波罗的海封闭股份公司 用于经控制dna片段化的方法
GB201405226D0 (en) 2014-03-24 2014-05-07 Cambridge Entpr Ltd Nucleic acid preparation method
GB201411603D0 (en) * 2014-06-30 2014-08-13 Vela Operations Pte Ltd Compositions for quantitative and/or semiquantitative mutation detection methods
ES2880335T3 (es) * 2014-09-09 2021-11-24 Igenomx Int Genomics Corporation Métodos y composiciones para la preparación rápida de bibliotecas de ácidos nucleicos
AU2015367290A1 (en) * 2014-12-16 2017-05-11 Garvan Institute Of Medical Research Sequencing controls
AU2016264102A1 (en) 2015-05-18 2017-12-21 Karius, Inc. Compositions and methods for enriching populations of nucleic acids
EP3405573A4 (en) * 2016-01-22 2019-09-18 Grail, Inc. METHOD AND SYSTEMS FOR HIGH-FIDELITY SEQUENCING
CA3185611A1 (en) 2016-03-25 2017-09-28 Karius, Inc. Synthetic nucleic acid spike-ins
AU2017292854B2 (en) 2016-07-06 2023-08-17 Guardant Health, Inc. Methods for fragmentome profiling of cell-free nucleic acids
EP3497218B1 (en) 2016-08-08 2022-03-16 Karius, Inc. Reduction of signal from contaminant nucleic acids
WO2018045359A1 (en) 2016-09-02 2018-03-08 Karius, Inc. Detection and treatment of infection during pregnancy
CA3041647A1 (en) 2016-10-24 2018-05-03 The Chinese University Of Hong Kong Methods and systems for tumor detection
WO2018187521A2 (en) 2017-04-06 2018-10-11 Cornell University Methods of detecting cell-free dna in biological samples
EP3610034B1 (en) 2017-04-12 2022-06-08 Karius, Inc. Sample preparation methods, systems and compositions
US12104205B2 (en) * 2017-06-20 2024-10-01 Mgi Tech Co., Ltd. PCR primer pair and application thereof
WO2019178157A1 (en) 2018-03-16 2019-09-19 Karius, Inc. Sample series to differentiate target nucleic acids from contaminant nucleic acids
EP3884065B1 (en) 2018-11-21 2024-08-14 Karius, Inc. Direct-to-library methods, systems, and compositions
CA3118742A1 (en) 2018-11-21 2020-05-28 Karius, Inc. Detection and prediction of infectious disease
US20220195496A1 (en) 2020-12-17 2022-06-23 Karius, Inc. Sequencing microbial cell-free dna from asymptomatic individuals

Also Published As

Publication number Publication date
US20210324467A1 (en) 2021-10-21
IL278000A (en) 2020-11-30
AU2017237199A1 (en) 2018-08-16
CN109312400A (zh) 2019-02-05
US9976181B2 (en) 2018-05-22
JP2022120165A (ja) 2022-08-17
KR102531487B1 (ko) 2023-05-10
BR112018069557A2 (pt) 2019-01-29
US11078532B2 (en) 2021-08-03
AU2021200637B2 (en) 2021-05-13
CA3185611A1 (en) 2017-09-28
CA3014070A1 (en) 2017-09-28
KR20210138154A (ko) 2021-11-18
KR102326769B1 (ko) 2021-11-17
EP3433382A4 (en) 2019-10-02
CA3014070C (en) 2023-03-14
EP3433382B1 (en) 2021-09-01
US20170275691A1 (en) 2017-09-28
KR102175718B1 (ko) 2020-11-06
EP3978627A1 (en) 2022-04-06
US11692224B2 (en) 2023-07-04
AU2024204822A1 (en) 2024-09-12
KR20200128194A (ko) 2020-11-11
MX2018011429A (es) 2019-06-06
US20180237851A1 (en) 2018-08-23
AU2021200637A1 (en) 2021-03-04
US20240043923A1 (en) 2024-02-08
EP3433382A1 (en) 2019-01-30
EP4198146A2 (en) 2023-06-21
IL278000B (en) 2022-04-01
JP2020182491A (ja) 2020-11-12
KR20180129844A (ko) 2018-12-05
CN116987777A (zh) 2023-11-03
JP7091397B2 (ja) 2022-06-27
EP4198146A3 (en) 2023-08-23
JP6743268B2 (ja) 2020-08-19
JP2019509068A (ja) 2019-04-04
WO2017165864A1 (en) 2017-09-28
MX2021006911A (es) 2021-07-07
AU2017237199B2 (en) 2020-11-05
IL260924B (en) 2021-01-31
AU2021215106A1 (en) 2021-08-26

Similar Documents

Publication Publication Date Title
MX2021006899A (es) A?adiduras de acidos nucleicos sinteticos.
MX2020000481A (es) Uso de proteína cas, método para detectar moléculas de ácido nucleico objetivo y kit.
WO2018119447A3 (en) Methods and systems for processing polynucleotides
EA201692157A1 (ru) Синтез двухцепочечных нуклеиновых кислот
GB2568608A (en) Personalized genetic testing
MX2016016898A (es) Metodos y composiciones para analisis de muestras.
WO2016130704A3 (en) Methods and compositions for analyzing cellular components
EP4397774A3 (en) Methods for barcoding nucleic acid molecules from individual cells or cell populations
WO2016130868A3 (en) Materials and methods to analyze rna isoforms in transcriptomes
GB2525804A (en) Methods and compositions for classification of samples
MY170164A (en) Aptamer-based multiplexed assays
MX2015016968A (es) Composiciones y metodos para procesamiento de muestras.
EA201892490A1 (ru) Пцр с мультиплексной оптимизированной амплификацией неправильно спаренных оснований (moma) в реальном времени для анализа состояния плода
WO2016127077A8 (en) Systems and methods for detection and analysis of biological species
TR201819546T4 (tr) Numune tür kökeni için tespit yöntemleri.
EP3543362A3 (en) Pcr primer set for bacterial dna amplification, kit for detecting and/or identifying bacterial species, and method for detecting and/or identifying bacterial species
ZA201806079B (en) Nucleic acid sequence for detecting existence of transgenic soybean event dbn9004 in biological sample, kit containing same and detection method therefor
MX2015012319A (es) Sistemas de instrumento analitico.
WO2014144561A3 (en) Lysophosphatidic acid detection
GB2554572A (en) Physical linkage preservation in DNA storage
WO2013090386A3 (en) Methods and kits for room temperature in situ detection of a target nucleic acid in a biological sample
GB2542576A (en) Method and kit of detecting the absence of micro-oranisms
CL2017001929A1 (es) Método y producto
MX2017001134A (es) Composiciones y metodos para la deteccion y analisis de tuberculosis micobacteriana.
WO2012106428A3 (en) Detection methods for target dna