MX2016010144A - Gangliosidos para estandarizacion y aumento de la sensibilidad de las celulas a las neurotoxinas botulinicas en sistemas de prueba in vitro. - Google Patents
Gangliosidos para estandarizacion y aumento de la sensibilidad de las celulas a las neurotoxinas botulinicas en sistemas de prueba in vitro.Info
- Publication number
- MX2016010144A MX2016010144A MX2016010144A MX2016010144A MX2016010144A MX 2016010144 A MX2016010144 A MX 2016010144A MX 2016010144 A MX2016010144 A MX 2016010144A MX 2016010144 A MX2016010144 A MX 2016010144A MX 2016010144 A MX2016010144 A MX 2016010144A
- Authority
- MX
- Mexico
- Prior art keywords
- pluripotent stem
- stem cell
- induced pluripotent
- derived neurons
- neurotoxin polypeptide
- Prior art date
Links
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
- G01N33/5058—Neurological cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0618—Cells of the nervous system
- C12N5/0619—Neurons
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
- G01N33/5073—Stem cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/36—Lipids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/50—Cell markers; Cell surface determinants
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/998—Proteins not provided for elsewhere
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/33—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Clostridium (G)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Neurology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Neurosurgery (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Toxicology (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Engineering & Computer Science (AREA)
- Developmental Biology & Embryology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
La presente invención se refiere a un método para la estandarización de la sensibilidad de las neuronas derivadas de las células madre pluripotentes inducidas (iPS) con respecto a un polipéptido de una neurotoxina, que comprende las etapas de: a) cultivar diferentes lotes de neuronas derivadas de las células madre pluripotentes inducidas en un medio de cultivo de las células que comprende GT1b durante al menos 3 horas; b) poner en contacto los diferentes lotes de las neuronas derivadas de las células madre pluripotentes inducidas de la etapa a) con un polipéptido de neurotoxina; c) cultivar los diferentes lotes de neuronas derivadas de las células madre pluripotentes inducidas de la etapa b) durante al menos 24 horas en la presencia de GT1b en condiciones que permitan al polipéptido de neurotoxina ejercer su actividad biológica estandarizando de este modo la sensibilidad de las neuronas derivadas de las células madre pluripotentes inducidas con respecto a un polipéptido de una neurotoxina. La invención se refiere además a un método para la generación de neuronas derivadas de las células madre pluripotentes inducidas que tienen una sensibilidad estandarizada con respecto a un polipéptido de neurotoxina, que comprende las etapas de: a) proporcionar diferentes lotes de las neuronas derivadas de las células madre pluripotentes inducidas; b) cultivar los diferentes lotes de las neuronas derivadas de las células madre pluripotentes inducidas de la etapa a) en un medio de cultivo celular que comprende GT1b durante al menos 3 horas, estandarizando de este modo la sensibilidad de las neuronas derivadas de las células madre pluripotentes inducidas con respecto a un polipéptido de neurotoxina. Además, está abarcado por la presente invención un método para determinar la actividad biológica de un polipéptido de neurotoxina, que comprende las etapas de: a) cultivar las neuronas derivadas de las células madre pluripotentes inducidas en un medio de cultivo celular que comprende GT1b durante al menos 3 horas; b) poner en contacto las neuronas derivadas de las células madre pluripotentes inducidas de la etapa a) con un polipéptido de neurotoxina; c) cultivar las neuronas derivadas de las células madre pluripotentes inducidas de la etapa b) durante al menos 24 horas en la presencia de GT1b bajo condiciones que permitan al polipéptido de neurotoxina ejercer su actividad biológica; y d) determinar la actividad biológica del polipéptido de neurotoxina en las células. Finalmente, la invención se refiere al uso de GT1b para a) la estandarización de la sensibilidad de los diferentes lotes de las neuronas derivadas de las células madre pluripotentes inducidas con respecto a un polipéptido de neurotoxina; o b) la reducción de la variabilidad de la sensibilidad de los diferentes lotes de neuronas derivadas de las células madre pluripotentes inducidas con respecto a un polipéptido de neurotoxina.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP14155726 | 2014-02-19 | ||
| PCT/EP2015/053403 WO2015124618A1 (en) | 2014-02-19 | 2015-02-18 | Gangliosides for standardizing and increasing the sensitivity of cells to botulinum neurotoxins in in vitro test systems |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2016010144A true MX2016010144A (es) | 2016-10-07 |
Family
ID=50115731
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2016010144A MX2016010144A (es) | 2014-02-19 | 2015-02-18 | Gangliosidos para estandarizacion y aumento de la sensibilidad de las celulas a las neurotoxinas botulinicas en sistemas de prueba in vitro. |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US10921312B2 (es) |
| EP (1) | EP3108243B1 (es) |
| JP (1) | JP6549599B2 (es) |
| KR (1) | KR102282266B1 (es) |
| CN (1) | CN106133522B (es) |
| AU (1) | AU2015220915B2 (es) |
| BR (1) | BR112016019104A2 (es) |
| CA (1) | CA2940082C (es) |
| ES (1) | ES2812769T3 (es) |
| HK (1) | HK1226481B (es) |
| IL (1) | IL247162A0 (es) |
| MX (1) | MX2016010144A (es) |
| RU (1) | RU2694191C2 (es) |
| SG (1) | SG11201606810SA (es) |
| WO (1) | WO2015124618A1 (es) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| LT3014267T (lt) | 2013-06-28 | 2018-12-10 | Merz Pharma Gmbh & Co. Kgaa | Priemonės ir būdai, skirti neurotoksinių polipeptidų nustatymui |
| JP7018955B2 (ja) * | 2017-10-03 | 2022-02-14 | オリンパス株式会社 | 培養情報処理装置 |
| CN117887797B (zh) * | 2023-12-27 | 2024-09-20 | 中国食品药品检定研究院 | 一种梭状芽胞杆菌神经毒素效价检测方法 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9508204D0 (en) * | 1995-04-21 | 1995-06-07 | Speywood Lab Ltd | A novel agent able to modify peripheral afferent function |
| EP2398897B1 (en) | 2009-02-20 | 2017-06-28 | Cellular Dynamics International, Inc. | Methods and compositions for the differentiation of stem cells |
| AU2010217739B2 (en) | 2009-02-27 | 2015-09-03 | FUJIFILM Cellular Dynamics, Inc. | Differentiation of pluripotent cells |
| JP5717657B2 (ja) | 2009-03-13 | 2015-05-13 | アラーガン、インコーポレイテッドＡｌｌｅｒｇａｎ，Ｉｎｃｏｒｐｏｒａｔｅｄ | 免疫系ボツリヌス毒素血清型ａ活性アッセイに有用な細胞 |
| US9487752B2 (en) | 2011-03-30 | 2016-11-08 | Cellular Dynamics International, Inc. | Priming of pluripotent stem cells for neural differentiation |
-
2015
- 2015-02-18 CN CN201580009292.8A patent/CN106133522B/zh active Active
- 2015-02-18 AU AU2015220915A patent/AU2015220915B2/en active Active
- 2015-02-18 CA CA2940082A patent/CA2940082C/en active Active
- 2015-02-18 ES ES15705022T patent/ES2812769T3/es active Active
- 2015-02-18 MX MX2016010144A patent/MX2016010144A/es active IP Right Grant
- 2015-02-18 SG SG11201606810SA patent/SG11201606810SA/en unknown
- 2015-02-18 JP JP2016552628A patent/JP6549599B2/ja active Active
- 2015-02-18 US US15/119,553 patent/US10921312B2/en active Active
- 2015-02-18 KR KR1020167024793A patent/KR102282266B1/ko active IP Right Grant
- 2015-02-18 RU RU2016131408A patent/RU2694191C2/ru active
- 2015-02-18 BR BR112016019104A patent/BR112016019104A2/pt not_active Application Discontinuation
- 2015-02-18 WO PCT/EP2015/053403 patent/WO2015124618A1/en active Application Filing
- 2015-02-18 EP EP15705022.0A patent/EP3108243B1/en active Active
-
2016
- 2016-08-08 IL IL247162A patent/IL247162A0/en active IP Right Grant
- 2016-12-23 HK HK16114641A patent/HK1226481B/zh unknown
Also Published As
| Publication number | Publication date |
|---|---|
| KR20160120752A (ko) | 2016-10-18 |
| RU2016131408A (ru) | 2018-03-22 |
| CN106133522B (zh) | 2018-10-12 |
| US10921312B2 (en) | 2021-02-16 |
| KR102282266B1 (ko) | 2021-07-27 |
| JP2017506892A (ja) | 2017-03-16 |
| EP3108243B1 (en) | 2020-07-15 |
| SG11201606810SA (en) | 2016-09-29 |
| AU2015220915A1 (en) | 2016-08-11 |
| US20170059558A1 (en) | 2017-03-02 |
| JP6549599B2 (ja) | 2019-07-24 |
| ES2812769T3 (es) | 2021-03-18 |
| CA2940082C (en) | 2023-09-19 |
| WO2015124618A1 (en) | 2015-08-27 |
| BR112016019104A2 (pt) | 2017-10-10 |
| RU2694191C2 (ru) | 2019-07-09 |
| AU2015220915B2 (en) | 2020-09-17 |
| CN106133522A (zh) | 2016-11-16 |
| HK1226481B (zh) | 2017-09-29 |
| CA2940082A1 (en) | 2015-08-27 |
| EP3108243A1 (en) | 2016-12-28 |
| RU2016131408A3 (es) | 2018-08-09 |
| IL247162A0 (en) | 2016-09-29 |
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| FG | Grant or registration |