BR112016019104A2 - método para padronizar a sensibilidade de neurônios, método para a geração de neurônios, método para determinar a atividade biológica, uso de gt1b - Google Patents

método para padronizar a sensibilidade de neurônios, método para a geração de neurônios, método para determinar a atividade biológica, uso de gt1b

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Publication number
BR112016019104A2
BR112016019104A2 BR112016019104A BR112016019104A BR112016019104A2 BR 112016019104 A2 BR112016019104 A2 BR 112016019104A2 BR 112016019104 A BR112016019104 A BR 112016019104A BR 112016019104 A BR112016019104 A BR 112016019104A BR 112016019104 A2 BR112016019104 A2 BR 112016019104A2
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BR
Brazil
Prior art keywords
pluripotent stem
neurons
derived
neurotoxin polypeptide
induced
Prior art date
Application number
BR112016019104A
Other languages
English (en)
Inventor
MANDER Gerd
Eisele Karl-Heinz
Original Assignee
Merz Pharma Gmbh &Co Kgaa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Merz Pharma Gmbh &Co Kgaa filed Critical Merz Pharma Gmbh &Co Kgaa
Publication of BR112016019104A2 publication Critical patent/BR112016019104A2/pt

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5058—Neurological cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5073—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/50—Cell markers; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/998—Proteins not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/33—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Clostridium (G)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Neurology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Neurosurgery (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Engineering & Computer Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

a presente invenção refere-se a um método para padronizar a sensibilidade de neurônios derivados de células-tronco pluripotentes induzidas (ips) a um polipeptídeo de neurotoxina que compreende as etapas: a) cultivar diferentes lotes de neurônios derivados de células-tronco pluripotentes induzidas em um meio de cultura celular que compreende gt1b por pelo menos 3 horas; b) colocar os diferentes lotes de neurônios derivados de células-tronco pluripotentes induzidas da etapa a) em contato com um polipeptídeo de neurotoxina; c) cultivar os diferentes lotes de neurônios derivados de células-tronco pluripotentes induzidas da etapa b) por pelo menos 24 horas na presença de gt1b sob condições que permitem que o polipeptídeo de neurotoxina exerça sua atividade biológica e, assim, padronizar a sensibilidade dos neurônios derivados de células-tronco pluripotentes induzidas a um polipeptídeo de neurotoxina. a invenção refere-se, ainda, a um método para a geração de neurônios derivados de células-tronco pluripotentes induzidas que têm sensibilidade padronizada a um polipeptídeo de neurotoxina, que compreende as etapas: a) fornecer diferentes lotes de neurônios derivados de células-tronco pluripotentes induzidas; b) cultivar diferentes lotes de neurônios derivados de células-tronco pluripotentes induzidas da etapa a) em um meio de cultura celular que compreende gt1b por pelo menos 3 horas e, assim, padronizar a sensibilidade dos neurônios derivados de células-tronco pluripotentes induzidas a um polipeptídeo de neurotoxina. adicionalmente, é abrangido pela presente invenção um método para determinar a atividade biológica de um polipeptídeo de uma neurotoxina que compreende as etapas: a) cultivar neurônios derivados de células-tronco pluripotentes induzidas em um meio de cultura celular que compreende gt1b por pelo menos 3 horas; b) colocar os neurônios derivados de células-tronco pluripotentes induzidas da etapa a) em contato com um polipeptídeo de neurotoxina; c) cultivar os neurônios derivados de células-tronco pluripotentes induzidas da etapa b) por pelo menos 24 horas na presença de gt1b sob condições que permitem que o polipeptídeo de neurotoxina exerça sua atividade biológica; e d) determinar a atividade biológica do polipeptídeo de neurotoxina nas ditas células. finalmente, a invenção refere-se ao uso de gt1b para a) padronizar a sensibilidade de diferentes lotes de neurônios derivados de células-tronco pluripotentes induzidas a um polipeptídeo de neurotoxina; ou b) reduzir a variabilidade da sensibilidade de diferentes lotes de neurônios derivados de células-tronco pluripotentes induzidas a um polipeptídeo de neurotoxina.
BR112016019104A 2014-02-19 2015-02-18 método para padronizar a sensibilidade de neurônios, método para a geração de neurônios, método para determinar a atividade biológica, uso de gt1b BR112016019104A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP14155726 2014-02-19
PCT/EP2015/053403 WO2015124618A1 (en) 2014-02-19 2015-02-18 Gangliosides for standardizing and increasing the sensitivity of cells to botulinum neurotoxins in in vitro test systems

Publications (1)

Publication Number Publication Date
BR112016019104A2 true BR112016019104A2 (pt) 2017-10-10

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ID=50115731

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016019104A BR112016019104A2 (pt) 2014-02-19 2015-02-18 método para padronizar a sensibilidade de neurônios, método para a geração de neurônios, método para determinar a atividade biológica, uso de gt1b

Country Status (15)

Country Link
US (1) US10921312B2 (pt)
EP (1) EP3108243B1 (pt)
JP (1) JP6549599B2 (pt)
KR (1) KR102282266B1 (pt)
CN (1) CN106133522B (pt)
AU (1) AU2015220915B2 (pt)
BR (1) BR112016019104A2 (pt)
CA (1) CA2940082C (pt)
ES (1) ES2812769T3 (pt)
HK (1) HK1226481B (pt)
IL (1) IL247162A0 (pt)
MX (1) MX2016010144A (pt)
RU (1) RU2694191C2 (pt)
SG (1) SG11201606810SA (pt)
WO (1) WO2015124618A1 (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105339790B (zh) 2013-06-28 2018-04-17 莫茨制药有限及两合公司 用于确定神经毒素多肽在细胞中的生物活性的工具和方法
JP7018955B2 (ja) 2017-10-03 2022-02-14 オリンパス株式会社 培養情報処理装置

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9508204D0 (en) * 1995-04-21 1995-06-07 Speywood Lab Ltd A novel agent able to modify peripheral afferent function
EP2398897B1 (en) 2009-02-20 2017-06-28 Cellular Dynamics International, Inc. Methods and compositions for the differentiation of stem cells
CA2753679C (en) 2009-02-27 2021-03-02 Cellular Dynamics International, Inc. Differentiation of pluripotent cells
KR102017327B1 (ko) 2009-03-13 2019-09-03 알러간, 인코포레이티드 면역 기반 보툴리눔 독소 혈청형 ａ 활성 검정에 유용한 세포
WO2012135621A2 (en) 2011-03-30 2012-10-04 Cellular Dynamics International. Inc Priming of pluripotent stem cells for neural differentiation

Also Published As

Publication number Publication date
KR20160120752A (ko) 2016-10-18
US10921312B2 (en) 2021-02-16
CN106133522B (zh) 2018-10-12
KR102282266B1 (ko) 2021-07-27
EP3108243B1 (en) 2020-07-15
EP3108243A1 (en) 2016-12-28
AU2015220915B2 (en) 2020-09-17
RU2016131408A (ru) 2018-03-22
CN106133522A (zh) 2016-11-16
RU2694191C2 (ru) 2019-07-09
CA2940082C (en) 2023-09-19
ES2812769T3 (es) 2021-03-18
SG11201606810SA (en) 2016-09-29
HK1226481B (zh) 2017-09-29
JP6549599B2 (ja) 2019-07-24
WO2015124618A1 (en) 2015-08-27
MX2016010144A (es) 2016-10-07
CA2940082A1 (en) 2015-08-27
AU2015220915A1 (en) 2016-08-11
JP2017506892A (ja) 2017-03-16
RU2016131408A3 (pt) 2018-08-09
IL247162A0 (en) 2016-09-29
US20170059558A1 (en) 2017-03-02

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B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]

Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NO 10196/2001, QUE MODIFICOU A LEI NO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUENCIA PREVIA DA ANVISA. CONSIDERANDO A APROVACAO DOS TERMOS DO PARECER NO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDENCIAS CABIVEIS.

B07E Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B12B Appeal against refusal [chapter 12.2 patent gazette]