MX2016009466A - Polipeptido de citocromo p450 novedoso con mayor actividad enzimatica. - Google Patents

Polipeptido de citocromo p450 novedoso con mayor actividad enzimatica.

Info

Publication number
MX2016009466A
MX2016009466A MX2016009466A MX2016009466A MX2016009466A MX 2016009466 A MX2016009466 A MX 2016009466A MX 2016009466 A MX2016009466 A MX 2016009466A MX 2016009466 A MX2016009466 A MX 2016009466A MX 2016009466 A MX2016009466 A MX 2016009466A
Authority
MX
Mexico
Prior art keywords
enzyme
polypeptide
enzymatic activity
nucleic acid
increased enzymatic
Prior art date
Application number
MX2016009466A
Other languages
English (en)
Inventor
Dumas Bruno
Bertin Marine
Original Assignee
Sanofi Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanofi Sa filed Critical Sanofi Sa
Publication of MX2016009466A publication Critical patent/MX2016009466A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • C12N9/0079—Steroid 11 beta monooxygenase (P-450 protein)(1.14.15.4)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/15—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen (1.14.15)
    • C12Y114/15004—Steroid 11-beta-monooxygenase (1.14.15.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y118/00—Oxidoreductases acting on iron-sulfur proteins as donors (1.18)
    • C12Y118/01—Oxidoreductases acting on iron-sulfur proteins as donors (1.18) with NAD+ or NADP+ as acceptor (1.18.1)
    • C12Y118/01002—Ferredoxin-NADP+ reductase (1.18.1.2)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Steroid Compounds (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invención se refiere a una enzima P450 aislada que comprende o consiste en una secuencia de aminoácidos idéntica en al menos 80% al SEQ ID NO: 1, en donde dicha secuencia comprende una treonina en la posición correspondiente a la posición 225 y/o una mutación de ácido aspártico en la posición correspondiente a la posición 289. La invención también se refiere a un ácido nucleico aislado que comprende una secuencia que codifica dicha enzima, un vector que comprende dicho ácido nucleico, y una célula anfitriona que contiene dicho ácido nucleico o dicho vector. También se proporcionan los métodos para la preparación de dicha enzima y los métodos para la producción de precursores de hormonas esteroides utilizando la enzima o las células anfitrionas expuestas en la invención.
MX2016009466A 2014-01-20 2015-01-19 Polipeptido de citocromo p450 novedoso con mayor actividad enzimatica. MX2016009466A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP14305071 2014-01-20
PCT/EP2015/050866 WO2015107185A1 (en) 2014-01-20 2015-01-19 Novel cytochrome p450 polypeptide with increased enzymatic activity

Publications (1)

Publication Number Publication Date
MX2016009466A true MX2016009466A (es) 2017-04-25

Family

ID=50028964

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016009466A MX2016009466A (es) 2014-01-20 2015-01-19 Polipeptido de citocromo p450 novedoso con mayor actividad enzimatica.

Country Status (17)

Country Link
US (2) US9765307B2 (es)
EP (1) EP3097113B1 (es)
JP (1) JP6608372B2 (es)
KR (1) KR20160108540A (es)
CN (1) CN106061996B (es)
AU (1) AU2015207519B2 (es)
BR (1) BR112016016586A2 (es)
CA (1) CA2936954A1 (es)
DK (1) DK3097113T3 (es)
ES (1) ES2724373T3 (es)
HU (1) HUE043216T2 (es)
IL (1) IL246801A0 (es)
MX (1) MX2016009466A (es)
RU (1) RU2677997C2 (es)
SG (1) SG11201605774QA (es)
TW (1) TW201613959A (es)
WO (1) WO2015107185A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111019913B (zh) * 2019-12-30 2023-07-07 苏州汇桢生物技术有限公司 一种p450scc突变体及核苷酸、表达载体、宿主细胞
CN112457412B (zh) * 2020-11-26 2022-10-14 浙江工业大学 一种人工电子传递系统及其在促进p450酶羟基化反应中的应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4861719A (en) 1986-04-25 1989-08-29 Fred Hutchinson Cancer Research Center DNA constructs for retrovirus packaging cell lines
US5278056A (en) 1988-02-05 1994-01-11 The Trustees Of Columbia University In The City Of New York Retroviral packaging cell lines and process of using same
IL90207A (en) * 1988-05-06 1994-07-31 Roussel Uclaf Biochemical oxidation of steroids and genetically engineered cells to be used therefor
US5670488A (en) 1992-12-03 1997-09-23 Genzyme Corporation Adenovirus vector for gene therapy
WO1994019478A1 (en) 1993-02-22 1994-09-01 The Rockefeller University Production of high titer helper-free retroviruses by transient transfection
FR2712812B1 (fr) 1993-11-23 1996-02-09 Centre Nat Rech Scient Composition pour la production de produits thérapeutiques in vivo.
IL116816A (en) 1995-01-20 2003-05-29 Rhone Poulenc Rorer Sa Cell for the production of a defective recombinant adenovirus or an adeno-associated virus and the various uses thereof
US6013516A (en) 1995-10-06 2000-01-11 The Salk Institute For Biological Studies Vector and method of use for nucleic acid delivery to non-dividing cells
CN1343779A (zh) * 2000-09-19 2002-04-10 上海博德基因开发有限公司 一种新的多肽——含有过氧化物酶特征的蛋白12.21和编码这种多肽的多核苷酸
CN1283800C (zh) * 2004-11-29 2006-11-08 浙江大学 能催化吲哚生成靛蓝的P450BM－3Glu435Asp变体基因及其用途
CN101333521B (zh) * 2008-04-25 2010-12-15 浙江大学 细胞色素p450bm-3d168w变体酶以及应用其制备靛玉红的方法
EP2673637A4 (en) * 2011-02-09 2014-12-31 Univ Temple DETERMINATION OF OOCYTE QUALITY

Also Published As

Publication number Publication date
SG11201605774QA (en) 2016-08-30
RU2016133705A3 (es) 2018-08-03
US20170342389A1 (en) 2017-11-30
RU2016133705A (ru) 2018-03-05
JP2017504334A (ja) 2017-02-09
IL246801A0 (en) 2016-08-31
KR20160108540A (ko) 2016-09-19
ES2724373T3 (es) 2019-09-10
US10155934B2 (en) 2018-12-18
JP6608372B2 (ja) 2019-11-20
US9765307B2 (en) 2017-09-19
EP3097113B1 (en) 2019-01-02
CA2936954A1 (en) 2015-07-23
TW201613959A (en) 2016-04-16
US20150225703A1 (en) 2015-08-13
CN106061996A (zh) 2016-10-26
AU2015207519A1 (en) 2016-08-18
WO2015107185A1 (en) 2015-07-23
HUE043216T2 (hu) 2019-08-28
CN106061996B (zh) 2021-03-09
AU2015207519B2 (en) 2018-11-08
EP3097113A1 (en) 2016-11-30
RU2677997C2 (ru) 2019-01-22
DK3097113T3 (en) 2019-04-23
BR112016016586A2 (pt) 2017-10-03

Similar Documents

Publication Publication Date Title
MX2023001119A (es) Variantes de enzimas y polinucleotidos que las codifican.
MX2019003674A (es) Enzimas modificadoras de ácido nucleico guiadas por arn y métodos de uso de estas.
WO2014202616A3 (en) Rasamsonia gene and use thereof
MX2015017110A (es) Integracion dirigida.
MY176331A (en) Carbohydrate degrading polypeptide and uses thereof
SG10201806498RA (en) Cultured mammalian limbal stem cells, methods for generating the same, and uses thereof
MX2021000210A (es) Metodos y productos para transfeccion de celulas.
MX2016009149A (es) Variantes mejoradas de enzimas.
MX2018009375A (es) Genes del factor viii optimizados.
MX2016008776A (es) Proteína de fusión de inhibe angiogénesis o crecimiento y uso de la misma.
MX2018010288A (es) Sistema de transposon y metodos de uso.
WO2016007570A3 (en) Engineered invariant natural killer t (inkt) cells and methods of making and using thereof
MX2020005458A (es) Variantes de alfa-amilasa y polinucleotidos que codifican las mismas.
NZ607627A (en) Mutated protoporphyrinogen ix oxidase (ppx) genes
NZ723978A (en) Genes involved in noscapine production
MX348776B (es) Produccion personalizada de productos biologicos y metodo para reprogramar celulas somaticas.
MX2019007248A (es) Enzimas de polimerizacion para reacciones de secuenciacion.
IN2014MN02500A (es)
MX2021013879A (es) Variantes de lipasa y polinucleotidos que las codifican.
MX2018011980A (es) 3-epimerasa y polinucleotido que la codifica.
MX2021012671A (es) Métodos y materiales para la producción de proteínas.
MX2017007105A (es) Variantes de lipasa y polinucleotidos que las codifican.
MX2017001824A (es) Mutaciones en proteinas del grupo hierro-azufre que mejoran la utilizacion de xilosa.
WO2015157431A3 (en) Selective advantage in fermentation
MX2018015534A (es) Beta-glucosidasas dise?adas y metodos de glucosilacion.