MX2015008107A - Transformacion de soja mejorada para una produccion aficaz de eventos transgenicos de alto rendimiento. - Google Patents

Transformacion de soja mejorada para una produccion aficaz de eventos transgenicos de alto rendimiento.

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Publication number
MX2015008107A
MX2015008107A MX2015008107A MX2015008107A MX2015008107A MX 2015008107 A MX2015008107 A MX 2015008107A MX 2015008107 A MX2015008107 A MX 2015008107A MX 2015008107 A MX2015008107 A MX 2015008107A MX 2015008107 A MX2015008107 A MX 2015008107A
Authority
MX
Mexico
Prior art keywords
efficient
transgenic event
soybean transformation
event production
improved soybean
Prior art date
Application number
MX2015008107A
Other languages
English (en)
Inventor
Olga Karpova
David Griffin
Tatyana Minnicks
Dayakar Pareddy
Jayakumar P Samuel
Kelley A Smith
Rodrigo Sarria-Millan
Tejinder Kumar Mall
Sivarama R Chennareddy
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=49956423&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2015008107(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of MX2015008107A publication Critical patent/MX2015008107A/es

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    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8281—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for bacterial resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance

Abstract

Se describe un método para la transformación de la de la línea germinal de la soja mediada por Agrobacterium, que comprende infectar semillas de soja divididas con una porción del eje embrionario con Agrobacterium tumefaciens que contiene un transgén. El método puede comprender además regenerar los explantes producidos a partir de la transformación de semillas de soja divididas que comprenden una porción de eje embrionario invitro en un medio de selección.
MX2015008107A 2012-12-19 2013-12-18 Transformacion de soja mejorada para una produccion aficaz de eventos transgenicos de alto rendimiento. MX2015008107A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261739349P 2012-12-19 2012-12-19
PCT/US2013/076230 WO2014100234A1 (en) 2012-12-19 2013-12-18 Improved soybean transformation for efficient and high-throughput transgenic event production

Publications (1)

Publication Number Publication Date
MX2015008107A true MX2015008107A (es) 2015-11-06

Family

ID=49956423

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2015008107A MX2015008107A (es) 2012-12-19 2013-12-18 Transformacion de soja mejorada para una produccion aficaz de eventos transgenicos de alto rendimiento.
MX2015008076A MX2015008076A (es) 2012-12-19 2013-12-19 Transformacion mejorada de soya en semilla dividida para la produccion eficiente y de alto rendimiento de evento transgenico.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2015008076A MX2015008076A (es) 2012-12-19 2013-12-19 Transformacion mejorada de soya en semilla dividida para la produccion eficiente y de alto rendimiento de evento transgenico.

Country Status (15)

Country Link
US (2) US20140173780A1 (es)
EP (2) EP2934094A4 (es)
JP (2) JP2016502853A (es)
KR (1) KR20150096739A (es)
CN (2) CN104869809A (es)
AR (2) AR094098A1 (es)
AU (1) AU2013361491A1 (es)
BR (2) BR102013032677A2 (es)
CA (2) CA2895212A1 (es)
CL (1) CL2015001736A1 (es)
IL (2) IL239383A0 (es)
MX (2) MX2015008107A (es)
PH (1) PH12015501415A1 (es)
WO (2) WO2014100234A1 (es)
ZA (1) ZA201503759B (es)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8524220B1 (en) 2010-02-09 2013-09-03 David Gordon Bermudes Protease inhibitor: protease sensitivity expression system composition and methods improving the therapeutic activity and specificity of proteins delivered by bacteria
US9597379B1 (en) 2010-02-09 2017-03-21 David Gordon Bermudes Protease inhibitor combination with therapeutic proteins including antibodies
US8771669B1 (en) 2010-02-09 2014-07-08 David Gordon Bermudes Immunization and/or treatment of parasites and infectious agents by live bacteria
AU2013361491A1 (en) 2012-12-19 2015-06-04 Dow Agrosciences Llc Improved soybean transformation for efficient and high-throughput transgenic event production
CN112301024A (zh) * 2013-03-15 2021-02-02 通用医疗公司 使用RNA引导的FokI核酸酶(RFN)提高RNA引导的基因组编辑的特异性
US9737592B1 (en) 2014-02-14 2017-08-22 David Gordon Bermudes Topical and orally administered protease inhibitors and bacterial vectors for the treatment of disorders and methods of treatment
US9980424B2 (en) * 2014-05-06 2018-05-29 Dow Agrosciences Llc System for seed preparation and method of use
US20150322443A1 (en) * 2014-05-06 2015-11-12 Dow Agrosciences Llc System for cutting and preparing seeds and method of use
MX2017016557A (es) * 2015-06-29 2018-05-11 Dow Agrosciences Llc Sistema para la preparacion automatica de explantes y metodos de uso.
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
CA3055396A1 (en) 2017-03-07 2018-09-13 BASF Agricultural Solutions Seed US LLC Hppd variants and methods of use
BR112019018175A2 (pt) 2017-03-07 2020-04-07 BASF Agricultural Solutions Seed US LLC molécula, célula, planta, sementes, polipeptídeos recombinantes, método para produzir um polipeptídeo, planta, método para controlar ervas, uso do ácido nucleico e produto de utilidade
US11453885B1 (en) * 2019-02-19 2022-09-27 Inari Agriculture Technology, Inc. Plant transformation
EP4025038A4 (en) * 2019-09-04 2023-09-13 Syngenta Crop Protection AG METHOD FOR TRANSFORMING THE CELLS OF DICOTYLED PLANTS
CA3157622A1 (en) * 2019-11-26 2021-06-03 Heng Zhong Methods of transformation

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JP2005535312A (ja) * 2002-06-22 2005-11-24 シンジェンタ パーティシペーションズ アクチェンゲゼルシャフト ダイズを形質転換する方法
UA90860C2 (ru) * 2004-06-07 2010-06-10 Басф Плант Саенс Гмбх Способ получения трансгенной сои
US7473822B1 (en) * 2005-06-27 2009-01-06 Iowa State University Research Foundation, Inc. Soybean transformation and regeneration using half-seed explant
EP3290520B1 (en) * 2007-03-09 2021-07-14 Monsanto Technology LLC Preparation and use of plant embryo explants for transformation
US20080229447A1 (en) * 2007-03-12 2008-09-18 Syngenta Participations Ag Transformation of immature soybean seeds through organogenesis
JP2008259497A (ja) * 2007-03-16 2008-10-30 Takano Foods Kk アグロバクテリウムを介したダイズ国内品種の形質転換体作出方法並びに形質転換体当代及び後代の種子を短期間で獲得する方法
CA2788052C (en) * 2010-02-02 2020-02-04 Bayer Cropscience Ag Soybean transformation using hppd inhibitors as selection agents
CN101974561A (zh) * 2010-11-23 2011-02-16 南京农业大学 农杆菌遗传转化大豆子叶节的方法
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CN102321666B (zh) * 2011-10-10 2013-12-18 浙江大学 双子叶植株转基因受体的快速制备方法
AU2013361491A1 (en) 2012-12-19 2015-06-04 Dow Agrosciences Llc Improved soybean transformation for efficient and high-throughput transgenic event production

Also Published As

Publication number Publication date
EP2935592B1 (en) 2018-03-21
CA2895343A1 (en) 2014-06-26
ZA201503759B (en) 2016-10-26
EP2934094A4 (en) 2016-06-22
MX2015008076A (es) 2016-03-21
AR094190A1 (es) 2015-07-15
PH12015501415A1 (en) 2015-09-07
IL239383A0 (en) 2015-07-30
BR102013032929A2 (pt) 2015-11-24
EP2934094A1 (en) 2015-10-28
CL2015001736A1 (es) 2016-04-22
US20140173774A1 (en) 2014-06-19
JP2016502853A (ja) 2016-02-01
EP2935592A1 (en) 2015-10-28
WO2014100406A1 (en) 2014-06-26
WO2014100234A1 (en) 2014-06-26
AR094098A1 (es) 2015-07-08
CA2895212A1 (en) 2014-06-26
BR102013032677A2 (pt) 2014-09-23
CN104869809A (zh) 2015-08-26
AU2013361491A1 (en) 2015-06-04
US20140173780A1 (en) 2014-06-19
JP2016502856A (ja) 2016-02-01
KR20150096739A (ko) 2015-08-25
CN105264077A (zh) 2016-01-20
IL239543A0 (en) 2015-08-31

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