MX2015008076A - Transformacion mejorada de soya en semilla dividida para la produccion eficiente y de alto rendimiento de evento transgenico. - Google Patents

Transformacion mejorada de soya en semilla dividida para la produccion eficiente y de alto rendimiento de evento transgenico.

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Publication number
MX2015008076A
MX2015008076A MX2015008076A MX2015008076A MX2015008076A MX 2015008076 A MX2015008076 A MX 2015008076A MX 2015008076 A MX2015008076 A MX 2015008076A MX 2015008076 A MX2015008076 A MX 2015008076A MX 2015008076 A MX2015008076 A MX 2015008076A
Authority
MX
Mexico
Prior art keywords
transformation
embryonic axis
soybean seeds
agrobacterium
efficient
Prior art date
Application number
MX2015008076A
Other languages
English (en)
Inventor
Dayakar Pareddy
Sivarama Reddy Chennareddy
Olga Karpova
David Griffin
Pon Samuel Jayakumar
Kelly Smith
Rodrigo Sarria-Milan
Tatyana Minnicks
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=49956423&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2015008076(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of MX2015008076A publication Critical patent/MX2015008076A/es

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    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8281—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for bacterial resistance
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Virology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Insects & Arthropods (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Beans For Foods Or Fodder (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Un método se divulga para la transformación de estirpe germinal agrobacterial de la soya, que comprende infectar las semillas de soya divididas, conservar una porción del eje embrionario, con Agrobacterium tumefaciens que contiene un transgén. El método puede además comprender la regeneración de los explantes producidos a partir de la transformación de las semillas de soya divididas que comprenden una porción del eje embrionario in vitro en medio de selección.
MX2015008076A 2012-12-19 2013-12-19 Transformacion mejorada de soya en semilla dividida para la produccion eficiente y de alto rendimiento de evento transgenico. MX2015008076A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261739349P 2012-12-19 2012-12-19
PCT/US2013/076540 WO2014100406A1 (en) 2012-12-19 2013-12-19 Improved split seed soybean transformation for efficient and high-throughput transgenic event production

Publications (1)

Publication Number Publication Date
MX2015008076A true MX2015008076A (es) 2016-03-21

Family

ID=49956423

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2015008107A MX2015008107A (es) 2012-12-19 2013-12-18 Transformacion de soja mejorada para una produccion aficaz de eventos transgenicos de alto rendimiento.
MX2015008076A MX2015008076A (es) 2012-12-19 2013-12-19 Transformacion mejorada de soya en semilla dividida para la produccion eficiente y de alto rendimiento de evento transgenico.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2015008107A MX2015008107A (es) 2012-12-19 2013-12-18 Transformacion de soja mejorada para una produccion aficaz de eventos transgenicos de alto rendimiento.

Country Status (15)

Country Link
US (2) US20140173780A1 (es)
EP (2) EP2934094A4 (es)
JP (2) JP2016502853A (es)
KR (1) KR20150096739A (es)
CN (2) CN104869809A (es)
AR (2) AR094098A1 (es)
AU (1) AU2013361491A1 (es)
BR (2) BR102013032677A2 (es)
CA (2) CA2895212A1 (es)
CL (1) CL2015001736A1 (es)
IL (2) IL239383A0 (es)
MX (2) MX2015008107A (es)
PH (1) PH12015501415A1 (es)
WO (2) WO2014100234A1 (es)
ZA (1) ZA201503759B (es)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9597379B1 (en) 2010-02-09 2017-03-21 David Gordon Bermudes Protease inhibitor combination with therapeutic proteins including antibodies
US8771669B1 (en) 2010-02-09 2014-07-08 David Gordon Bermudes Immunization and/or treatment of parasites and infectious agents by live bacteria
US8524220B1 (en) 2010-02-09 2013-09-03 David Gordon Bermudes Protease inhibitor: protease sensitivity expression system composition and methods improving the therapeutic activity and specificity of proteins delivered by bacteria
AU2013361491A1 (en) 2012-12-19 2015-06-04 Dow Agrosciences Llc Improved soybean transformation for efficient and high-throughput transgenic event production
CA2906553C (en) * 2013-03-15 2022-08-02 The General Hospital Corporation Rna-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci
US9737592B1 (en) 2014-02-14 2017-08-22 David Gordon Bermudes Topical and orally administered protease inhibitors and bacterial vectors for the treatment of disorders and methods of treatment
MX2016014312A (es) * 2014-05-06 2017-04-27 Dow Agrosciences Llc Sistema para preparacion de semillas y metodo de uso.
US20150322443A1 (en) * 2014-05-06 2015-11-12 Dow Agrosciences Llc System for cutting and preparing seeds and method of use
JP2018521648A (ja) * 2015-06-29 2018-08-09 ダウ アグロサイエンシィズ エルエルシー 自動化された外植片調製のためのシステム及び使用方法
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11180770B2 (en) 2017-03-07 2021-11-23 BASF Agricultural Solutions Seed US LLC HPPD variants and methods of use
BR112019018059A2 (pt) 2017-03-07 2020-08-04 BASF Agricultural Solutions Seed US LLC molécula de ácido nucleico recombinante, célula hospedeira, plantas, sementes transgênicas, polipeptídeo recombinante, método para produzir um polipeptídeo, método de controle de ervas daninhas, uso do ácido nucleico e produto de utilidade
US11453885B1 (en) * 2019-02-19 2022-09-27 Inari Agriculture Technology, Inc. Plant transformation
CN114727584A (zh) * 2019-09-04 2022-07-08 先正达农作物保护股份公司 双子叶植物细胞的转化方法
CN115605082A (zh) * 2019-11-26 2023-01-13 先正达农作物保护股份公司（Ch） 转化方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2180079B (en) * 1984-12-22 1989-03-30 Yaskawa Denki Seisakusho Kk Servo motor control method and apparatus
US5015580A (en) * 1987-07-29 1991-05-14 Agracetus Particle-mediated transformation of soybean plants and lines
CA2359868A1 (en) * 1999-01-14 2000-07-20 Monsanto Company Soybean transformation method
CA2490154A1 (en) * 2002-06-22 2003-12-31 Syngenta Participations Ag Method of transforming soybean
UA90860C2 (ru) * 2004-06-07 2010-06-10 Басф Плант Саенс Гмбх Способ получения трансгенной сои
US7473822B1 (en) * 2005-06-27 2009-01-06 Iowa State University Research Foundation, Inc. Soybean transformation and regeneration using half-seed explant
US8044260B2 (en) * 2007-03-09 2011-10-25 Monsanto Technology Llc Method of meristem excision and transformation
US20080229447A1 (en) * 2007-03-12 2008-09-18 Syngenta Participations Ag Transformation of immature soybean seeds through organogenesis
JP2008259497A (ja) * 2007-03-16 2008-10-30 Takano Foods Kk アグロバクテリウムを介したダイズ国内品種の形質転換体作出方法並びに形質転換体当代及び後代の種子を短期間で獲得する方法
AR080105A1 (es) * 2010-02-02 2012-03-14 Bayer Cropscience Ag Transformacion de soja usando inhibidores de hidrofenil piruvato dioxigenasa (hppd) como agentes de seleccion
CN101974561A (zh) * 2010-11-23 2011-02-16 南京农业大学 农杆菌遗传转化大豆子叶节的方法
CN102174562A (zh) * 2011-01-28 2011-09-07 河北农业大学 一种新型生根方法在大豆转基因技术中的应用
CN102321666B (zh) * 2011-10-10 2013-12-18 浙江大学 双子叶植株转基因受体的快速制备方法
AU2013361491A1 (en) 2012-12-19 2015-06-04 Dow Agrosciences Llc Improved soybean transformation for efficient and high-throughput transgenic event production

Also Published As

Publication number Publication date
WO2014100234A1 (en) 2014-06-26
CN105264077A (zh) 2016-01-20
US20140173780A1 (en) 2014-06-19
CL2015001736A1 (es) 2016-04-22
EP2935592B1 (en) 2018-03-21
ZA201503759B (en) 2016-10-26
AR094190A1 (es) 2015-07-15
JP2016502853A (ja) 2016-02-01
PH12015501415A1 (en) 2015-09-07
WO2014100406A1 (en) 2014-06-26
EP2934094A4 (en) 2016-06-22
EP2935592A1 (en) 2015-10-28
AR094098A1 (es) 2015-07-08
CN104869809A (zh) 2015-08-26
CA2895343A1 (en) 2014-06-26
IL239383A0 (en) 2015-07-30
KR20150096739A (ko) 2015-08-25
CA2895212A1 (en) 2014-06-26
EP2934094A1 (en) 2015-10-28
MX2015008107A (es) 2015-11-06
AU2013361491A1 (en) 2015-06-04
IL239543A0 (en) 2015-08-31
US20140173774A1 (en) 2014-06-19
BR102013032677A2 (pt) 2014-09-23
JP2016502856A (ja) 2016-02-01
BR102013032929A2 (pt) 2015-11-24

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