MX2015005206A - Metodos de cultivo de perfusion y aplicaciones de los mismos. - Google Patents
Metodos de cultivo de perfusion y aplicaciones de los mismos.Info
- Publication number
- MX2015005206A MX2015005206A MX2015005206A MX2015005206A MX2015005206A MX 2015005206 A MX2015005206 A MX 2015005206A MX 2015005206 A MX2015005206 A MX 2015005206A MX 2015005206 A MX2015005206 A MX 2015005206A MX 2015005206 A MX2015005206 A MX 2015005206A
- Authority
- MX
- Mexico
- Prior art keywords
- degrees
- culture medium
- liquid culture
- rpm
- volume
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/50—Means for positioning or orientating the apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Clinical Laboratory Science (AREA)
- Sustainable Development (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
En el presente documento se proporcionan los métodos mejorados para cultivar una célula mamífera que incluyen proporcionar un envase cónico que contiene una célula mamífera suspendida en aproximadamente 4% a aproximadamente 80% de volumen del envase de un primer medio de cultivo líquido; incubar el envase durante un periodo de tiempo a una temperatura de aproximadamente 31°C a aproximadamente 40°C a un ángulo de reactor de aproximadamente 5 grados a aproximadamente 85 grados (por ejemplo, de aproximadamente 40 grados a aproximadamente 55 grados) desde el plano horizontal y con una agitación de aproximadamente 20 revoluciones por minuto (RPM, por sus siglas en inglés) a aproximadamente 1000 RPM; y de manera continua o periódica, durante el periodo de tiempo, retirar un primer volumen del primer medio de cultivo líquido que está sustancialmente libre de las células mamíferas y agregar al primer medio de cultivo líquido el segundo volumen de un segundo medio de cultivo líquido, donde el primer y el segundo volumen son aproximadamente iguales, y los métodos para cultivar una célula mamífera incluyen cultivar, en un proceso de perfusión en gradiente, una célula mamífera suspendida en un medio de cultivo líquido bajo las condiciones que generan en el medio una fuerza cortante de fluido y una concentración de oxígeno (O2) que es igual que (o esencialmente igual que) aquella que se alcanza en un envase cónico permeable al gas que contiene de 4% a 40% de volumen del envase de medio de cultivo líquido cuando se coloca a un ángulo de reactor de aproximadamente 5 grados a aproximadamente 85 grados (por ejemplo, de aproximadamente 40 grados a aproximadamente 55 grados) desde el plano horizontal, incubado a una temperatura de aproximadamente 31°C a aproximadamente 40°C, y agitado a una frecuencia de aproximadamente 20 revoluciones por minuto (RPM, por sus siglas en inglés) a aproximadamente 1000 RPM. También se proporcionan varios métodos que utilizan estos métodos de cultivo.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201261717486P | 2012-10-23 | 2012-10-23 | |
PCT/US2013/066410 WO2014066519A1 (en) | 2012-10-23 | 2013-10-23 | Perfusion culturing methods and uses thereof |
Publications (2)
Publication Number | Publication Date |
---|---|
MX2015005206A true MX2015005206A (es) | 2016-08-03 |
MX363702B MX363702B (es) | 2019-03-29 |
Family
ID=49546634
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2015005206A MX363702B (es) | 2012-10-23 | 2013-10-23 | Metodos de cultivo de perfusion y aplicaciones de los mismos. |
MX2019003638A MX2019003638A (es) | 2012-10-23 | 2015-04-23 | Metodos de cultivo de perfusion y aplicaciones de los mismos. |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2019003638A MX2019003638A (es) | 2012-10-23 | 2015-04-23 | Metodos de cultivo de perfusion y aplicaciones de los mismos. |
Country Status (9)
Country | Link |
---|---|
US (1) | US20140154726A1 (es) |
EP (2) | EP2912160B1 (es) |
CN (2) | CN104903436B (es) |
AU (2) | AU2013334602B2 (es) |
CA (1) | CA2889022A1 (es) |
HK (1) | HK1214292A1 (es) |
MX (2) | MX363702B (es) |
SG (2) | SG10201901708SA (es) |
WO (1) | WO2014066519A1 (es) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
MX367025B (es) * | 2013-02-22 | 2019-08-02 | Genzyme Corp | Metodos de cultivo por perfusion con microportadores y usos de los mismos. |
ES2717927T3 (es) | 2013-02-22 | 2019-06-26 | Genzyme Corp | Procedimientos de cultivo por perfusión de microportadores y usos de los mismos |
AR095196A1 (es) | 2013-03-15 | 2015-09-30 | Regeneron Pharma | Medio de cultivo celular libre de suero |
TWI743024B (zh) * | 2014-06-06 | 2021-10-21 | 美商健臻公司 | 灌注培養方法及其用途 |
TWI776235B (zh) | 2014-06-09 | 2022-09-01 | 美商健臻公司 | 種子罐培養法(seed train processes)及其用途 |
HUE060385T2 (hu) | 2014-10-24 | 2023-02-28 | Genzyme Corp | Fluidumáramok integrált folyamatos elszigetelése steril folyamatedényektõl |
BR112017013423B1 (pt) | 2014-12-22 | 2023-04-11 | Genzyme Corporation | Métodos para cultivo de célula de mamífero |
CN105385731B (zh) * | 2015-12-25 | 2018-10-30 | 上海莱士血液制品股份有限公司 | 一种表达重组八因子的灌注培养方法 |
KR20180047404A (ko) * | 2016-10-31 | 2018-05-10 | 삼성바이오에피스 주식회사 | 세포 배양 배지에서 원치 않는 배양 부산물을 감소시키는 공정 |
EP3601584A1 (en) * | 2017-03-31 | 2020-02-05 | Boehringer Ingelheim International GmbH | Perfusion medium |
MA50911A (fr) * | 2017-11-13 | 2020-09-23 | Amgen Inc | Procédés de production de produits protéiniques |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2866655B1 (fr) * | 2004-02-20 | 2007-05-11 | Cesco Bioengineering Co Ltd | Appareil et procede pour la preparation et la mise en culture de cellules |
WO2006138143A1 (en) * | 2005-06-15 | 2006-12-28 | Amprotein Corporation | Suspension culture vessels |
JP2008543307A (ja) * | 2005-06-15 | 2008-12-04 | アンプロテイン コーポレイション | 懸濁培養用容器 |
US20080206819A1 (en) * | 2006-08-21 | 2008-08-28 | Mary Tsao | Intensified Perfusion Production Method |
WO2009034186A2 (en) * | 2007-09-13 | 2009-03-19 | Helmholtz-Zentrum für Infektionsforschung GmbH | Process for cell cultivation |
US20090233334A1 (en) * | 2008-03-11 | 2009-09-17 | Excellgene Sa | Cell cultivation and production of recombinant proteins by means of an orbital shake bioreactor system with disposable bags at the 1,500 liter scale |
US20100076380A1 (en) * | 2008-06-16 | 2010-03-25 | Amprotein Corporation | Bioreactors |
US8728479B2 (en) | 2009-03-31 | 2014-05-20 | The Trustees Of The University Of Pennsylvania | Antigen-binding proteins comprising recombinant protein scaffolds |
-
2013
- 2013-10-23 WO PCT/US2013/066410 patent/WO2014066519A1/en active Application Filing
- 2013-10-23 MX MX2015005206A patent/MX363702B/es unknown
- 2013-10-23 US US14/061,657 patent/US20140154726A1/en not_active Abandoned
- 2013-10-23 EP EP13786587.9A patent/EP2912160B1/en active Active
- 2013-10-23 AU AU2013334602A patent/AU2013334602B2/en not_active Ceased
- 2013-10-23 CA CA2889022A patent/CA2889022A1/en not_active Abandoned
- 2013-10-23 SG SG10201901708SA patent/SG10201901708SA/en unknown
- 2013-10-23 EP EP20188132.3A patent/EP3747984A1/en not_active Withdrawn
- 2013-10-23 CN CN201380067513.8A patent/CN104903436B/zh not_active Expired - Fee Related
- 2013-10-23 SG SG11201503085VA patent/SG11201503085VA/en unknown
- 2013-10-23 CN CN201810153403.8A patent/CN108192822A/zh active Pending
-
2015
- 2015-04-23 MX MX2019003638A patent/MX2019003638A/es unknown
-
2016
- 2016-03-02 HK HK16102431.5A patent/HK1214292A1/zh unknown
-
2018
- 2018-08-30 AU AU2018222985A patent/AU2018222985A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
CN108192822A (zh) | 2018-06-22 |
SG10201901708SA (en) | 2019-03-28 |
HK1214292A1 (zh) | 2016-07-22 |
EP3747984A1 (en) | 2020-12-09 |
MX363702B (es) | 2019-03-29 |
EP2912160B1 (en) | 2020-07-29 |
AU2018222985A1 (en) | 2018-09-20 |
CN104903436B (zh) | 2018-03-30 |
CA2889022A1 (en) | 2014-05-01 |
US20140154726A1 (en) | 2014-06-05 |
AU2013334602B2 (en) | 2018-05-31 |
MX2019003638A (es) | 2019-09-02 |
AU2013334602A1 (en) | 2015-05-14 |
WO2014066519A1 (en) | 2014-05-01 |
EP2912160A1 (en) | 2015-09-02 |
CN104903436A (zh) | 2015-09-09 |
SG11201503085VA (en) | 2015-05-28 |
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