MX2015005206A - Metodos de cultivo de perfusion y aplicaciones de los mismos. - Google Patents

Metodos de cultivo de perfusion y aplicaciones de los mismos.

Info

Publication number
MX2015005206A
MX2015005206A MX2015005206A MX2015005206A MX2015005206A MX 2015005206 A MX2015005206 A MX 2015005206A MX 2015005206 A MX2015005206 A MX 2015005206A MX 2015005206 A MX2015005206 A MX 2015005206A MX 2015005206 A MX2015005206 A MX 2015005206A
Authority
MX
Mexico
Prior art keywords
degrees
culture medium
liquid culture
rpm
volume
Prior art date
Application number
MX2015005206A
Other languages
English (en)
Other versions
MX363702B (es
Inventor
Yang Yang
Yang Jianguo
Villiger-Oberbek Agata
Original Assignee
Genzyme Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genzyme Corp filed Critical Genzyme Corp
Publication of MX2015005206A publication Critical patent/MX2015005206A/es
Publication of MX363702B publication Critical patent/MX363702B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/50—Means for positioning or orientating the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

En el presente documento se proporcionan los métodos mejorados para cultivar una célula mamífera que incluyen proporcionar un envase cónico que contiene una célula mamífera suspendida en aproximadamente 4% a aproximadamente 80% de volumen del envase de un primer medio de cultivo líquido; incubar el envase durante un periodo de tiempo a una temperatura de aproximadamente 31°C a aproximadamente 40°C a un ángulo de reactor de aproximadamente 5 grados a aproximadamente 85 grados (por ejemplo, de aproximadamente 40 grados a aproximadamente 55 grados) desde el plano horizontal y con una agitación de aproximadamente 20 revoluciones por minuto (RPM, por sus siglas en inglés) a aproximadamente 1000 RPM; y de manera continua o periódica, durante el periodo de tiempo, retirar un primer volumen del primer medio de cultivo líquido que está sustancialmente libre de las células mamíferas y agregar al primer medio de cultivo líquido el segundo volumen de un segundo medio de cultivo líquido, donde el primer y el segundo volumen son aproximadamente iguales, y los métodos para cultivar una célula mamífera incluyen cultivar, en un proceso de perfusión en gradiente, una célula mamífera suspendida en un medio de cultivo líquido bajo las condiciones que generan en el medio una fuerza cortante de fluido y una concentración de oxígeno (O2) que es igual que (o esencialmente igual que) aquella que se alcanza en un envase cónico permeable al gas que contiene de 4% a 40% de volumen del envase de medio de cultivo líquido cuando se coloca a un ángulo de reactor de aproximadamente 5 grados a aproximadamente 85 grados (por ejemplo, de aproximadamente 40 grados a aproximadamente 55 grados) desde el plano horizontal, incubado a una temperatura de aproximadamente 31°C a aproximadamente 40°C, y agitado a una frecuencia de aproximadamente 20 revoluciones por minuto (RPM, por sus siglas en inglés) a aproximadamente 1000 RPM. También se proporcionan varios métodos que utilizan estos métodos de cultivo.
MX2015005206A 2012-10-23 2013-10-23 Metodos de cultivo de perfusion y aplicaciones de los mismos. MX363702B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261717486P 2012-10-23 2012-10-23
PCT/US2013/066410 WO2014066519A1 (en) 2012-10-23 2013-10-23 Perfusion culturing methods and uses thereof

Publications (2)

Publication Number Publication Date
MX2015005206A true MX2015005206A (es) 2016-08-03
MX363702B MX363702B (es) 2019-03-29

Family

ID=49546634

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2015005206A MX363702B (es) 2012-10-23 2013-10-23 Metodos de cultivo de perfusion y aplicaciones de los mismos.
MX2019003638A MX2019003638A (es) 2012-10-23 2015-04-23 Metodos de cultivo de perfusion y aplicaciones de los mismos.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2019003638A MX2019003638A (es) 2012-10-23 2015-04-23 Metodos de cultivo de perfusion y aplicaciones de los mismos.

Country Status (9)

Country Link
US (1) US20140154726A1 (es)
EP (2) EP2912160B1 (es)
CN (2) CN104903436B (es)
AU (2) AU2013334602B2 (es)
CA (1) CA2889022A1 (es)
HK (1) HK1214292A1 (es)
MX (2) MX363702B (es)
SG (2) SG10201901708SA (es)
WO (1) WO2014066519A1 (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX367025B (es) * 2013-02-22 2019-08-02 Genzyme Corp Metodos de cultivo por perfusion con microportadores y usos de los mismos.
ES2717927T3 (es) 2013-02-22 2019-06-26 Genzyme Corp Procedimientos de cultivo por perfusión de microportadores y usos de los mismos
AR095196A1 (es) 2013-03-15 2015-09-30 Regeneron Pharma Medio de cultivo celular libre de suero
TWI743024B (zh) * 2014-06-06 2021-10-21 美商健臻公司 灌注培養方法及其用途
TWI776235B (zh) 2014-06-09 2022-09-01 美商健臻公司 種子罐培養法(seed train processes)及其用途
HUE060385T2 (hu) 2014-10-24 2023-02-28 Genzyme Corp Fluidumáramok integrált folyamatos elszigetelése steril folyamatedényektõl
BR112017013423B1 (pt) 2014-12-22 2023-04-11 Genzyme Corporation Métodos para cultivo de célula de mamífero
CN105385731B (zh) * 2015-12-25 2018-10-30 上海莱士血液制品股份有限公司 一种表达重组八因子的灌注培养方法
KR20180047404A (ko) * 2016-10-31 2018-05-10 삼성바이오에피스 주식회사 세포 배양 배지에서 원치 않는 배양 부산물을 감소시키는 공정
EP3601584A1 (en) * 2017-03-31 2020-02-05 Boehringer Ingelheim International GmbH Perfusion medium
MA50911A (fr) * 2017-11-13 2020-09-23 Amgen Inc Procédés de production de produits protéiniques

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2866655B1 (fr) * 2004-02-20 2007-05-11 Cesco Bioengineering Co Ltd Appareil et procede pour la preparation et la mise en culture de cellules
WO2006138143A1 (en) * 2005-06-15 2006-12-28 Amprotein Corporation Suspension culture vessels
JP2008543307A (ja) * 2005-06-15 2008-12-04 アンプロテイン コーポレイション 懸濁培養用容器
US20080206819A1 (en) * 2006-08-21 2008-08-28 Mary Tsao Intensified Perfusion Production Method
WO2009034186A2 (en) * 2007-09-13 2009-03-19 Helmholtz-Zentrum für Infektionsforschung GmbH Process for cell cultivation
US20090233334A1 (en) * 2008-03-11 2009-09-17 Excellgene Sa Cell cultivation and production of recombinant proteins by means of an orbital shake bioreactor system with disposable bags at the 1,500 liter scale
US20100076380A1 (en) * 2008-06-16 2010-03-25 Amprotein Corporation Bioreactors
US8728479B2 (en) 2009-03-31 2014-05-20 The Trustees Of The University Of Pennsylvania Antigen-binding proteins comprising recombinant protein scaffolds

Also Published As

Publication number Publication date
CN108192822A (zh) 2018-06-22
SG10201901708SA (en) 2019-03-28
HK1214292A1 (zh) 2016-07-22
EP3747984A1 (en) 2020-12-09
MX363702B (es) 2019-03-29
EP2912160B1 (en) 2020-07-29
AU2018222985A1 (en) 2018-09-20
CN104903436B (zh) 2018-03-30
CA2889022A1 (en) 2014-05-01
US20140154726A1 (en) 2014-06-05
AU2013334602B2 (en) 2018-05-31
MX2019003638A (es) 2019-09-02
AU2013334602A1 (en) 2015-05-14
WO2014066519A1 (en) 2014-05-01
EP2912160A1 (en) 2015-09-02
CN104903436A (zh) 2015-09-09
SG11201503085VA (en) 2015-05-28

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