MX2015000683A - Metodo para producir moleculas monomericas y multimericas y uso de las mismas. - Google Patents

Metodo para producir moleculas monomericas y multimericas y uso de las mismas.

Info

Publication number
MX2015000683A
MX2015000683A MX2015000683A MX2015000683A MX2015000683A MX 2015000683 A MX2015000683 A MX 2015000683A MX 2015000683 A MX2015000683 A MX 2015000683A MX 2015000683 A MX2015000683 A MX 2015000683A MX 2015000683 A MX2015000683 A MX 2015000683A
Authority
MX
Mexico
Prior art keywords
polypeptide
denotes
mer
region
biologically active
Prior art date
Application number
MX2015000683A
Other languages
English (en)
Inventor
Stefan Seeber
Johannes Auer
Martin Bader
Stefan Lorenz
Stefan Dengl
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of MX2015000683A publication Critical patent/MX2015000683A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C07K14/7155—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for interleukins [IL]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/0004—Screening or testing of compounds for diagnosis of disorders, assessment of conditions, e.g. renal clearance, gastric emptying, testing for diabetes, allergy, rheuma, pancreas functions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/525—Tumour necrosis factor [TNF]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/71—Decreased effector function due to an Fc-modification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

Abstract

Aquí se reporta un método para la producción de un polipéptido que es activo biológicamente como n-mer que comprende un ácido nucléico que codifica un polipéptido de fusión de acuerdo con la siguiente fórmula (Bn-CS0-Is-CSp-FC-CSq-It-CSr-Bm)u, en donde B denota un polipéptido que es biológicamente activo como n-mer y forma agregados/multímeros no definidos durante la expresión en la ausencia de una región de Fc fusionada, FC denota un polipéptido de la región de Fc de cadena pesada, CS denota un sitio de escisión, e I denota una secuencia de aminoácidos de intervención, en donde FC no se aglutina substancialmente a un receptor de Fc, recuperar el polipéptido de fusión de la célula o del medio de cultivo, escindiendo opcionalmente el polipéptido de fusión con una proteasa, y producir por esto un polipéptido que es biológicamente activo como n-mer y forma agregados/multímeros no definidos durante la expresión en la ausencia de una región de Fc fusionada.
MX2015000683A 2012-08-02 2013-07-31 Metodo para producir moleculas monomericas y multimericas y uso de las mismas. MX2015000683A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP12179021 2012-08-02
PCT/EP2013/066096 WO2014020069A1 (en) 2012-08-02 2013-07-31 Method for producing monomeric and multimeric molecules and uses thereof

Publications (1)

Publication Number Publication Date
MX2015000683A true MX2015000683A (es) 2015-04-10

Family

ID=48914281

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2015000683A MX2015000683A (es) 2012-08-02 2013-07-31 Metodo para producir moleculas monomericas y multimericas y uso de las mismas.

Country Status (13)

Country Link
US (3) US20150218250A1 (es)
EP (1) EP2880169B1 (es)
JP (1) JP6388581B2 (es)
KR (1) KR20150037959A (es)
CN (1) CN104508133B (es)
BR (1) BR112015002091A2 (es)
CA (1) CA2876099A1 (es)
ES (1) ES2633894T3 (es)
HK (1) HK1208880A1 (es)
MX (1) MX2015000683A (es)
RU (1) RU2015106812A (es)
SG (1) SG11201408530YA (es)
WO (1) WO2014020069A1 (es)

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AU2015226100B2 (en) 2014-03-05 2020-05-07 UCB Biopharma SRL Multimeric Fc proteins
WO2016010014A1 (ja) 2014-07-15 2016-01-21 アステラス製薬株式会社 新規抗ヒトＴｉｅ２抗体
RU2713131C1 (ru) * 2014-11-06 2020-02-03 Ф. Хоффманн-Ля Рош Аг ВАРИАНТЫ Fc-ОБЛАСТИ С МОДИФИЦИРОВАННЫМИ СВОЙСТВАМИ СВЯЗЫВАНИЯ FcRn И БЕЛКА А
US11566082B2 (en) 2014-11-17 2023-01-31 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
ES2874974T3 (es) 2016-05-11 2021-11-05 Cytiva Bioprocess R & D Ab Matriz de separación
US10654887B2 (en) 2016-05-11 2020-05-19 Ge Healthcare Bio-Process R&D Ab Separation matrix
CN109311948B (zh) 2016-05-11 2022-09-16 思拓凡生物工艺研发有限公司 清洁和/或消毒分离基质的方法
JP7106187B2 (ja) 2016-05-11 2022-07-26 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 分離マトリックスを保存する方法
US10889615B2 (en) 2016-05-11 2021-01-12 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
US10703774B2 (en) 2016-09-30 2020-07-07 Ge Healthcare Bioprocess R&D Ab Separation method
US10730908B2 (en) 2016-05-11 2020-08-04 Ge Healthcare Bioprocess R&D Ab Separation method
EP3492166A4 (en) * 2016-08-01 2020-03-11 Kaneka Corporation ADSORPTION AGENT FOR CALCIPROTE PARTICLES, ADSORPTION REMOVAL SYSTEM AND METHOD FOR USE THEREOF
CA3033475A1 (en) 2016-08-10 2018-02-15 Ajou University Industry-Academic Cooperation Foundation Heterodimeric fc-fused cytokine and pharmaceutical composition comprising the same
CN116970060A (zh) * 2016-12-22 2023-10-31 库尔生物制药有限公司 T细胞调节性多聚体多肽及其使用方法
JP7356726B2 (ja) * 2017-09-25 2023-10-05 ディンフー バイオターゲット カンパニー リミテッド タンパク性ヘテロ二量体及びその使用
MA52366A (fr) 2018-04-25 2021-03-03 Prometheus Biosciences Inc Anticorps anti-tl1a optimisés
PE20211279A1 (es) 2018-10-23 2021-07-19 Dragonfly Therapeutics Inc Proteinas heterodimericas fusionadas con fc
KR20220103721A (ko) 2019-10-24 2022-07-22 프로메테우스 바이오사이언시즈, 인크. Tnf 유사 리간드 1a(tl1a)에 대한 인간화 항체 및 그의 용도
CN116348481A (zh) * 2020-08-19 2023-06-27 詹森生物科技公司 使用工程化配体的材料和方法

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Also Published As

Publication number Publication date
US20170342128A1 (en) 2017-11-30
US20150218250A1 (en) 2015-08-06
US20200157183A1 (en) 2020-05-21
ES2633894T3 (es) 2017-09-25
CA2876099A1 (en) 2014-02-06
US11254728B2 (en) 2022-02-22
WO2014020069A1 (en) 2014-02-06
EP2880169B1 (en) 2017-05-17
US10570188B2 (en) 2020-02-25
JP6388581B2 (ja) 2018-09-12
BR112015002091A2 (pt) 2017-12-12
CN104508133A (zh) 2015-04-08
RU2015106812A (ru) 2016-09-27
EP2880169A1 (en) 2015-06-10
JP2015531591A (ja) 2015-11-05
CN104508133B (zh) 2018-04-20
HK1208880A1 (en) 2016-03-18
KR20150037959A (ko) 2015-04-08
SG11201408530YA (en) 2015-03-30

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