MX2014001918A - Metodo de cromatografia de intercambio cationico y anionico. - Google Patents
Metodo de cromatografia de intercambio cationico y anionico.Info
- Publication number
- MX2014001918A MX2014001918A MX2014001918A MX2014001918A MX2014001918A MX 2014001918 A MX2014001918 A MX 2014001918A MX 2014001918 A MX2014001918 A MX 2014001918A MX 2014001918 A MX2014001918 A MX 2014001918A MX 2014001918 A MX2014001918 A MX 2014001918A
- Authority
- MX
- Mexico
- Prior art keywords
- exchange chromatography
- polypeptide
- solution
- ion exchange
- chromatography material
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/775—Apolipopeptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
- B01D15/362—Cation-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
- B01D15/363—Anion-exchange
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
La presente invención se relaciona con un método para purificar un polipéptido, que comprende las etapas de: i) - aplicar una solución que comprende el polipéptido a un material de cromatografía de intercambio iónico, y ii) - recuperar el polipéptido con una solución que comprende un desnaturalizante y, de esta manera, purificar el polipéptido, por lo que el material de cromatografía de intercambio iónico comprende una matriz de poli (estireno-divinilbenceno) reticulado al que los ligandos iónicos se han enlazado, y en donde la solución que comprende el polipéptido aplicado al material de cromatografía de intercambio iónico está libre del desnaturalizante y el polipéptido absorbido al material de cromatografía de intercambio iónico se recupera con una solución que comprende un desnaturalizante a una conductividad constante.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP11178745 | 2011-08-25 | ||
| EP11183862 | 2011-10-04 | ||
| PCT/EP2012/066135 WO2013026803A1 (en) | 2011-08-25 | 2012-08-17 | Cation and anion exchange chromatography method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2014001918A true MX2014001918A (es) | 2014-04-14 |
Family
ID=46717838
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2014001918A MX2014001918A (es) | 2011-08-25 | 2012-08-17 | Metodo de cromatografia de intercambio cationico y anionico. |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US20130053551A1 (es) |
| EP (1) | EP2748178B1 (es) |
| JP (1) | JP6116565B2 (es) |
| KR (1) | KR20140062044A (es) |
| CN (1) | CN103703015B (es) |
| BR (1) | BR112014004463A2 (es) |
| CA (1) | CA2838814A1 (es) |
| MX (1) | MX2014001918A (es) |
| RU (1) | RU2014108241A (es) |
| WO (1) | WO2013026803A1 (es) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8791063B2 (en) | 2011-08-25 | 2014-07-29 | Hoffmann-La Roche, Inc. | Shortened tetranectin-apolipoprotein A-I fusion protein, a lipid particle containing it, and uses thereof |
| CN103980351B (zh) * | 2014-05-27 | 2016-05-18 | 上海第一生化药业有限公司 | 加压素、加压素鞣酸盐的制备方法 |
| EP3591574A3 (en) * | 2018-07-06 | 2020-01-15 | Universität Zürich | Method and computer program for clustering large multiplexed spatially resolved data of a biological sample |
| CN109851667A (zh) * | 2018-12-29 | 2019-06-07 | 江苏万邦医药科技有限公司 | 一种德谷胰岛素前体的纯化方法 |
Family Cites Families (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2629568C3 (de) | 1976-07-01 | 1981-09-10 | Hoechst Ag, 6000 Frankfurt | Verfahren zur Reinigung von Insulin, seinen Analogen und Derivaten |
| AU5397579A (en) | 1978-12-26 | 1980-07-03 | Eli Lilly And Company | Purifying insulin |
| US5118796A (en) * | 1987-12-09 | 1992-06-02 | Centocor, Incorporated | Efficient large-scale purification of immunoglobulins and derivatives |
| DE4118912C1 (es) | 1991-06-08 | 1992-07-02 | Biotest Pharma Gmbh, 6072 Dreieich, De | |
| DE4124896C1 (es) | 1991-07-26 | 1993-01-21 | Eloma Gmbh Bedarfsartikel Zur Gemeinschaftsverpflegung, 8031 Maisach, De | |
| US5463029A (en) * | 1992-11-23 | 1995-10-31 | Immunex Corporation | Purification of fusion proteins comprising GM-CSF and IL-3 |
| US6451987B1 (en) | 1999-03-15 | 2002-09-17 | Novo Nordisk A/S | Ion exchange chromatography of proteins and peptides |
| EP2343317A1 (en) | 2000-11-10 | 2011-07-13 | F. Hoffmann-La Roche Ltd. | Apolipoprotein analogues |
| EP1501369B1 (en) * | 2002-04-26 | 2015-06-24 | Genentech, Inc. | Non-affinity purification of proteins |
| US20040048315A1 (en) * | 2002-08-28 | 2004-03-11 | Pharmacia Corporation | Method for the preparation of growth hormone and antagonist thereof having lower levels of isoform impurities thereof |
| ES2629602T5 (es) | 2002-09-11 | 2021-06-08 | Genentech Inc | Purificación de proteínas |
| ES2337041T3 (es) * | 2002-09-20 | 2010-04-20 | Pharmacia Corporation | Procedimiento para disminuir los niveles de agregacion de proteina pegilada. |
| GB0224082D0 (en) * | 2002-10-16 | 2002-11-27 | Celltech R&D Ltd | Biological products |
| EP2001898A1 (en) * | 2006-03-20 | 2008-12-17 | Medarex Inc. | Protein purification |
| US20090242750A1 (en) * | 2006-06-30 | 2009-10-01 | Ge Healthcare Bio-Sciences Ab | Protein purification and identification |
| US8013122B2 (en) * | 2006-12-20 | 2011-09-06 | Kieu Hoang | Method of purifying apolipoprotein A-1 |
| CN100586958C (zh) | 2006-12-20 | 2010-02-03 | 上海莱士血液制品股份有限公司 | 高纯度载脂蛋白a-i的制备方法 |
| BRPI0922716A2 (pt) * | 2008-12-02 | 2016-11-01 | Novo Nordisk Healthcare Ag | purificação de polipeptídeo |
| AU2010351576B2 (en) * | 2010-04-23 | 2014-08-14 | FinaBioSolutions, LLC | Simple method for simultaneous removal of multiple impurities from culture supernatants to ultralow levels |
| TW201201840A (en) | 2010-07-15 | 2012-01-16 | Hoffmann La Roche | Antibodies specifically binding to human TSLPR and methods of use |
| JP2013535978A (ja) | 2010-08-30 | 2013-09-19 | エフ．ホフマン−ラ ロシュ アーゲー | 原核生物における発現構築物 |
| WO2012028525A2 (en) | 2010-08-30 | 2012-03-08 | F. Hoffmann-La Roche Ag | Method for producing a lipid particle, the lipid particle itself and its use |
| ES2558751T3 (es) | 2010-08-30 | 2016-02-08 | F. Hoffmann-La Roche Ag | Alimento alcalino |
| US9187550B2 (en) | 2010-08-30 | 2015-11-17 | Hoffman—La Roche Inc. | Tetranectin-apolipoprotein A-I, lipid particles containing it and its use |
-
2012
- 2012-08-17 CA CA2838814A patent/CA2838814A1/en not_active Abandoned
- 2012-08-17 MX MX2014001918A patent/MX2014001918A/es unknown
- 2012-08-17 WO PCT/EP2012/066135 patent/WO2013026803A1/en active Application Filing
- 2012-08-17 KR KR1020147004660A patent/KR20140062044A/ko not_active Application Discontinuation
- 2012-08-17 JP JP2014526457A patent/JP6116565B2/ja active Active
- 2012-08-17 EP EP12748689.2A patent/EP2748178B1/en active Active
- 2012-08-17 BR BR112014004463A patent/BR112014004463A2/pt not_active IP Right Cessation
- 2012-08-17 US US13/588,281 patent/US20130053551A1/en not_active Abandoned
- 2012-08-17 RU RU2014108241/04A patent/RU2014108241A/ru not_active Application Discontinuation
- 2012-08-17 CN CN201280036823.9A patent/CN103703015B/zh active Active
-
2015
- 2015-10-22 US US14/920,127 patent/US11332514B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| BR112014004463A2 (pt) | 2017-03-28 |
| EP2748178A1 (en) | 2014-07-02 |
| CN103703015A (zh) | 2014-04-02 |
| CN103703015B (zh) | 2019-07-05 |
| US20130053551A1 (en) | 2013-02-28 |
| RU2014108241A (ru) | 2015-09-27 |
| CA2838814A1 (en) | 2013-02-28 |
| JP2014524453A (ja) | 2014-09-22 |
| JP6116565B2 (ja) | 2017-04-19 |
| WO2013026803A1 (en) | 2013-02-28 |
| KR20140062044A (ko) | 2014-05-22 |
| EP2748178B1 (en) | 2018-10-31 |
| US20160237140A1 (en) | 2016-08-18 |
| US11332514B2 (en) | 2022-05-17 |
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