MX2014001918A - Metodo de cromatografia de intercambio cationico y anionico. - Google Patents

Metodo de cromatografia de intercambio cationico y anionico.

Info

Publication number
MX2014001918A
MX2014001918A MX2014001918A MX2014001918A MX2014001918A MX 2014001918 A MX2014001918 A MX 2014001918A MX 2014001918 A MX2014001918 A MX 2014001918A MX 2014001918 A MX2014001918 A MX 2014001918A MX 2014001918 A MX2014001918 A MX 2014001918A
Authority
MX
Mexico
Prior art keywords
exchange chromatography
polypeptide
solution
ion exchange
chromatography material
Prior art date
Application number
MX2014001918A
Other languages
English (en)
Inventor
Roberto Falkenstein
Maria Laura Magri
Michaela Mehr
Klaus Schwendner
Bernhard Spensberger
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of MX2014001918A publication Critical patent/MX2014001918A/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/775Apolipopeptides
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • C07K1/18Ion-exchange chromatography
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361Ion-exchange
    • B01D15/362Cation-exchange
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361Ion-exchange
    • B01D15/363Anion-exchange

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención se relaciona con un método para purificar un polipéptido, que comprende las etapas de: i) - aplicar una solución que comprende el polipéptido a un material de cromatografía de intercambio iónico, y ii) - recuperar el polipéptido con una solución que comprende un desnaturalizante y, de esta manera, purificar el polipéptido, por lo que el material de cromatografía de intercambio iónico comprende una matriz de poli (estireno-divinilbenceno) reticulado al que los ligandos iónicos se han enlazado, y en donde la solución que comprende el polipéptido aplicado al material de cromatografía de intercambio iónico está libre del desnaturalizante y el polipéptido absorbido al material de cromatografía de intercambio iónico se recupera con una solución que comprende un desnaturalizante a una conductividad constante.
MX2014001918A 2011-08-25 2012-08-17 Metodo de cromatografia de intercambio cationico y anionico. MX2014001918A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP11178745 2011-08-25
EP11183862 2011-10-04
PCT/EP2012/066135 WO2013026803A1 (en) 2011-08-25 2012-08-17 Cation and anion exchange chromatography method

Publications (1)

Publication Number Publication Date
MX2014001918A true MX2014001918A (es) 2014-04-14

Family

ID=46717838

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014001918A MX2014001918A (es) 2011-08-25 2012-08-17 Metodo de cromatografia de intercambio cationico y anionico.

Country Status (10)

Country Link
US (2) US20130053551A1 (es)
EP (1) EP2748178B1 (es)
JP (1) JP6116565B2 (es)
KR (1) KR20140062044A (es)
CN (1) CN103703015B (es)
BR (1) BR112014004463A2 (es)
CA (1) CA2838814A1 (es)
MX (1) MX2014001918A (es)
RU (1) RU2014108241A (es)
WO (1) WO2013026803A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6207507B2 (ja) 2011-08-25 2017-10-04 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 短縮されたテトラネクチン−アポリポプロテインa−i融合タンパク質、それを含む脂質粒子、及びその使用
CN103980351B (zh) * 2014-05-27 2016-05-18 上海第一生化药业有限公司 加压素、加压素鞣酸盐的制备方法
EP3591574A3 (en) * 2018-07-06 2020-01-15 Universität Zürich Method and computer program for clustering large multiplexed spatially resolved data of a biological sample
CN109851667A (zh) * 2018-12-29 2019-06-07 江苏万邦医药科技有限公司 一种德谷胰岛素前体的纯化方法

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DE2629568C3 (de) * 1976-07-01 1981-09-10 Hoechst Ag, 6000 Frankfurt Verfahren zur Reinigung von Insulin, seinen Analogen und Derivaten
AU5397579A (en) 1978-12-26 1980-07-03 Eli Lilly And Company Purifying insulin
US5118796A (en) 1987-12-09 1992-06-02 Centocor, Incorporated Efficient large-scale purification of immunoglobulins and derivatives
DE4118912C1 (es) 1991-06-08 1992-07-02 Biotest Pharma Gmbh, 6072 Dreieich, De
DE4124896C1 (es) 1991-07-26 1993-01-21 Eloma Gmbh Bedarfsartikel Zur Gemeinschaftsverpflegung, 8031 Maisach, De
US5463029A (en) * 1992-11-23 1995-10-31 Immunex Corporation Purification of fusion proteins comprising GM-CSF and IL-3
US6451987B1 (en) 1999-03-15 2002-09-17 Novo Nordisk A/S Ion exchange chromatography of proteins and peptides
JP4344136B2 (ja) 2000-11-10 2009-10-14 エフ・ホフマン−ラ・ロシュ・リミテッド アポリポタンパク質類似体
JP4319979B2 (ja) * 2002-04-26 2009-08-26 ジェネンテック・インコーポレーテッド タンパク質の非アフィニティ精製
US20040048315A1 (en) * 2002-08-28 2004-03-11 Pharmacia Corporation Method for the preparation of growth hormone and antagonist thereof having lower levels of isoform impurities thereof
LT1543038T (lt) 2002-09-11 2017-07-10 Genentech, Inc. Baltymo gryninimas
DE60330787D1 (de) * 2002-09-20 2010-02-11 Pharmacia Corp Verfahren zur verringerung der aggregatmengen des pegylierten proteins
GB0224082D0 (en) 2002-10-16 2002-11-27 Celltech R&D Ltd Biological products
AU2007227700A1 (en) * 2006-03-20 2007-09-27 Medarex, Inc. Protein purification
US20090242750A1 (en) * 2006-06-30 2009-10-01 Ge Healthcare Bio-Sciences Ab Protein purification and identification
US8013122B2 (en) * 2006-12-20 2011-09-06 Kieu Hoang Method of purifying apolipoprotein A-1
CN100586958C (zh) 2006-12-20 2010-02-03 上海莱士血液制品股份有限公司 高纯度载脂蛋白a-i的制备方法
ES2576109T3 (es) * 2008-12-02 2016-07-05 Novo Nordisk Health Care Ag Purificación de polipéptidos
WO2011133191A1 (en) * 2010-04-23 2011-10-27 Serum Institute Of India, Ltd. Simple method for simultaneous removal of multiple impurities from culture supernatants to ultralow levels
AR082163A1 (es) 2010-07-15 2012-11-14 Hoffmann La Roche Anticuerpos especificamente ligantes del tslpr humano y metodos de utilizacion de los mismos
ES2558751T3 (es) 2010-08-30 2016-02-08 F. Hoffmann-La Roche Ag Alimento alcalino
WO2012028523A2 (en) 2010-08-30 2012-03-08 F. Hoffmann-La Roche Ag Prokaryotic expression construct
WO2012028526A2 (en) 2010-08-30 2012-03-08 F. Hoffmann-La Roche Ag Tetranectin-apolipoprotein a-i, lipid particles containing it and its use
RU2013111676A (ru) 2010-08-30 2014-10-10 Ф. Хоффманн-Ля Рош Аг Способ получения тетранектин-аполипопротеин а-i частицы, полученная таким образом липидная частица и ее применение

Also Published As

Publication number Publication date
US11332514B2 (en) 2022-05-17
EP2748178A1 (en) 2014-07-02
KR20140062044A (ko) 2014-05-22
BR112014004463A2 (pt) 2017-03-28
CA2838814A1 (en) 2013-02-28
EP2748178B1 (en) 2018-10-31
US20130053551A1 (en) 2013-02-28
US20160237140A1 (en) 2016-08-18
JP6116565B2 (ja) 2017-04-19
RU2014108241A (ru) 2015-09-27
CN103703015B (zh) 2019-07-05
WO2013026803A1 (en) 2013-02-28
JP2014524453A (ja) 2014-09-22
CN103703015A (zh) 2014-04-02

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