BR112014004463A2 - método de cromatografia de troca de cátion e de ânion - Google Patents

método de cromatografia de troca de cátion e de ânion

Info

Publication number
BR112014004463A2
BR112014004463A2 BR112014004463A BR112014004463A BR112014004463A2 BR 112014004463 A2 BR112014004463 A2 BR 112014004463A2 BR 112014004463 A BR112014004463 A BR 112014004463A BR 112014004463 A BR112014004463 A BR 112014004463A BR 112014004463 A2 BR112014004463 A2 BR 112014004463A2
Authority
BR
Brazil
Prior art keywords
exchange chromatography
polypeptide
solution
cation
ion exchange
Prior art date
Application number
BR112014004463A
Other languages
English (en)
Inventor
Spensberger Bernhard
Schwendner Klaus
laura magri Maria
Mehr Michaela
Falkenstein Roberto
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of BR112014004463A2 publication Critical patent/BR112014004463A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/775—Apolipopeptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

resumo “método de cromatografia de troca de cátion e de ânion” aqui é relatado um método para purificar um polipeptídeo compreendendo as etapas de i) aplicar uma solução compreendendo o polipeptídeo em um material de 5 cromatografia de troca iônica, e ii) recuperar o polipeptídeo com uma solução compreendendo um desnaturante e desta forma purificar o polipeptídeo, em que o material de cromatografia de troca iônica compreende uma matriz de poli (estireno-divinilbenzeno) reticulado ao qual os 10 ligantes iônicos foram anexados, e em que a solução compreendendo o polipeptídeo aplicado ao material de cromatografia de troca iônica está livre de desnaturante e o polipeptídeo adsorvido ao material de cromatografia de troca iônica é recuperado com uma solução compreendendo um 15 desnaturante em uma condutividade constante.
BR112014004463A 2011-08-25 2012-08-17 método de cromatografia de troca de cátion e de ânion BR112014004463A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP11178745 2011-08-25
EP11183862 2011-10-04
PCT/EP2012/066135 WO2013026803A1 (en) 2011-08-25 2012-08-17 Cation and anion exchange chromatography method

Publications (1)

Publication Number Publication Date
BR112014004463A2 true BR112014004463A2 (pt) 2017-03-28

Family

ID=46717838

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014004463A BR112014004463A2 (pt) 2011-08-25 2012-08-17 método de cromatografia de troca de cátion e de ânion

Country Status (10)

Country Link
US (2) US20130053551A1 (pt)
EP (1) EP2748178B1 (pt)
JP (1) JP6116565B2 (pt)
KR (1) KR20140062044A (pt)
CN (1) CN103703015B (pt)
BR (1) BR112014004463A2 (pt)
CA (1) CA2838814A1 (pt)
MX (1) MX2014001918A (pt)
RU (1) RU2014108241A (pt)
WO (1) WO2013026803A1 (pt)

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* Cited by examiner, † Cited by third party
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KR20140054115A (ko) 2011-08-25 2014-05-08 에프. 호프만-라 로슈 아게 단축 테트라넥틴-아포지단백질 a-i 융합 단백질, 그를 함유하는 지질 입자 및 그의 용도
CN103980351B (zh) * 2014-05-27 2016-05-18 上海第一生化药业有限公司 加压素、加压素鞣酸盐的制备方法
EP3591574A3 (en) * 2018-07-06 2020-01-15 Universität Zürich Method and computer program for clustering large multiplexed spatially resolved data of a biological sample
CN109851667A (zh) * 2018-12-29 2019-06-07 江苏万邦医药科技有限公司 一种德谷胰岛素前体的纯化方法

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DE2629568C3 (de) 1976-07-01 1981-09-10 Hoechst Ag, 6000 Frankfurt Verfahren zur Reinigung von Insulin, seinen Analogen und Derivaten
AU5397579A (en) 1978-12-26 1980-07-03 Eli Lilly And Company Purifying insulin
US5118796A (en) 1987-12-09 1992-06-02 Centocor, Incorporated Efficient large-scale purification of immunoglobulins and derivatives
DE4118912C1 (pt) 1991-06-08 1992-07-02 Biotest Pharma Gmbh, 6072 Dreieich, De
DE4124896C1 (pt) 1991-07-26 1993-01-21 Eloma Gmbh Bedarfsartikel Zur Gemeinschaftsverpflegung, 8031 Maisach, De
US5463029A (en) * 1992-11-23 1995-10-31 Immunex Corporation Purification of fusion proteins comprising GM-CSF and IL-3
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US20040048315A1 (en) 2002-08-28 2004-03-11 Pharmacia Corporation Method for the preparation of growth hormone and antagonist thereof having lower levels of isoform impurities thereof
HUE033623T2 (en) 2002-09-11 2017-12-28 Genentech Inc protein purification
DE60330787D1 (de) * 2002-09-20 2010-02-11 Pharmacia Corp Verfahren zur verringerung der aggregatmengen des pegylierten proteins
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CA2645739A1 (en) * 2006-03-20 2007-09-27 Medarex, Inc. Protein purification
WO2008002235A1 (en) * 2006-06-30 2008-01-03 Ge Healthcare Bio-Sciences Ab Protein purification and identification
US8013122B2 (en) * 2006-12-20 2011-09-06 Kieu Hoang Method of purifying apolipoprotein A-1
CN100586958C (zh) 2006-12-20 2010-02-03 上海莱士血液制品股份有限公司 高纯度载脂蛋白a-i的制备方法
WO2010063717A1 (en) 2008-12-02 2010-06-10 Novo Nordisk A/S Polypeptide purification
NZ602971A (en) * 2010-04-23 2014-11-28 Serum Inst India Ltd Simple method for simultaneous removal of multiple impurities from culture supernatants to ultralow levels
TW201201840A (en) 2010-07-15 2012-01-16 Hoffmann La Roche Antibodies specifically binding to human TSLPR and methods of use
BR112013004396A2 (pt) 2010-08-30 2016-05-17 Hoffmann La Roche alimentação alcalina
KR20130117760A (ko) 2010-08-30 2013-10-28 에프. 호프만-라 로슈 아게 테트라넥틴-아포지단백질 a-i 입자의 제조 방법, 이에 의해 제조된 지질 입자 및 이의 용도
RU2013112562A (ru) 2010-08-30 2014-10-10 Ф. Хоффманн-Ля Рош Аг Тетранектин-аполипопротеин а-i, содержащие его липидные частицы и его применение
US8877895B2 (en) 2010-08-30 2014-11-04 Hoffmann-La Roche Inc. Prokaryotic expression construct

Also Published As

Publication number Publication date
EP2748178B1 (en) 2018-10-31
US11332514B2 (en) 2022-05-17
CA2838814A1 (en) 2013-02-28
MX2014001918A (es) 2014-04-14
WO2013026803A1 (en) 2013-02-28
CN103703015A (zh) 2014-04-02
JP2014524453A (ja) 2014-09-22
KR20140062044A (ko) 2014-05-22
CN103703015B (zh) 2019-07-05
RU2014108241A (ru) 2015-09-27
EP2748178A1 (en) 2014-07-02
JP6116565B2 (ja) 2017-04-19
US20160237140A1 (en) 2016-08-18
US20130053551A1 (en) 2013-02-28

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]
B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2475 DE 12-06-2018 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.