MX2010011900A - Método de cuantificación de aminoácidos por cromatografía líquida de alta resolución (hplc) a partir de una fermentación láctica. - Google Patents

Método de cuantificación de aminoácidos por cromatografía líquida de alta resolución (hplc) a partir de una fermentación láctica.

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Publication number
MX2010011900A
MX2010011900A MX2010011900A MX2010011900A MX2010011900A MX 2010011900 A MX2010011900 A MX 2010011900A MX 2010011900 A MX2010011900 A MX 2010011900A MX 2010011900 A MX2010011900 A MX 2010011900A MX 2010011900 A MX2010011900 A MX 2010011900A
Authority
MX
Mexico
Prior art keywords
amino acids
phase
lactic fermentation
water
hplc
Prior art date
Application number
MX2010011900A
Other languages
English (en)
Inventor
Jaime Lopez Cervantes
Dalia Isabel Sanchez Machado
Karl Reiner Fick Rochin
Original Assignee
Bioderpac S A De C V
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioderpac S A De C V filed Critical Bioderpac S A De C V
Priority to MX2010011900A priority Critical patent/MX2010011900A/es
Priority to PCT/MX2011/000017 priority patent/WO2012057598A1/es
Publication of MX2010011900A publication Critical patent/MX2010011900A/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G01N30/14—Preparation by elimination of some components
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G01N2030/065—Preparation using different phases to separate parts of sample
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G01N2030/067—Preparation by reaction, e.g. derivatising the sample
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G01N30/14—Preparation by elimination of some components
    • G01N2030/143—Preparation by elimination of some components selective absorption
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/30—Control of physical parameters of the fluid carrier of temperature
    • G01N2030/3007—Control of physical parameters of the fluid carrier of temperature same temperature for whole column
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/74—Optical detectors

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención trata de un método HPLC para la cuantificación de aminoácidos libres en el hidrolizado de cabeza de camarón obtenido mediante fermentación láctica, para su análisis las muestras fueron derivatizadas con 9-fluorenil metil cloroformato (FMOC) y detección por fluorescencia (ex: 270 nm, em: 316 nm) en una columna SGE Hypersil ODS C18 250 mm x 4.6 mm, 5 m, se utilizaron tres soluciones como fase móvil para el análisis: fase A: 30 mM fosfato de amonio (pH 6.5) en 15:85 metanol/agua, Fase B: 15:85 metanol/agua y la fase C: 90:10 acetonitrilo/agua, con un flujo de bomba de 1.20 mL/min, con una temperatura de columna de 38 °C. El método fue utilizado para cuantificar los aminoácidos presentes en el licor obtenido como producto final en la fermentación láctica. Se encontró que la linealidad, precisión y recuperación del método son adecuadas para la cuantificación de los aminoácidos seleccionados, las muestras analizadas contienen 20.40, 24.8 y 18.8 µg/g materia seca de alanina, valina e isoleucina respectivamente.
MX2010011900A 2010-10-29 2010-10-29 Método de cuantificación de aminoácidos por cromatografía líquida de alta resolución (hplc) a partir de una fermentación láctica. MX2010011900A (es)

Priority Applications (2)

Application Number Priority Date Filing Date Title
MX2010011900A MX2010011900A (es) 2010-10-29 2010-10-29 Método de cuantificación de aminoácidos por cromatografía líquida de alta resolución (hplc) a partir de una fermentación láctica.
PCT/MX2011/000017 WO2012057598A1 (es) 2010-10-29 2011-02-04 Método de cuantificación de aminoácidos por cromatografía líquida de alta resolución (hplc) a partir de una fermentación láctica

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MX2010011900A MX2010011900A (es) 2010-10-29 2010-10-29 Método de cuantificación de aminoácidos por cromatografía líquida de alta resolución (hplc) a partir de una fermentación láctica.

Publications (1)

Publication Number Publication Date
MX2010011900A true MX2010011900A (es) 2012-04-30

Family

ID=45994129

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2010011900A MX2010011900A (es) 2010-10-29 2010-10-29 Método de cuantificación de aminoácidos por cromatografía líquida de alta resolución (hplc) a partir de una fermentación láctica.

Country Status (2)

Country Link
MX (1) MX2010011900A (es)
WO (1) WO2012057598A1 (es)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112578044A (zh) * 2020-12-02 2021-03-30 成都普康生物科技有限公司 一种高效液相检测 Fmoc-AEEA含量的方法
CN114441681A (zh) * 2022-01-28 2022-05-06 山东省食品药品检验研究院 一种测定特殊医学配方食品中20种游离氨基酸和l-羟脯氨酸的方法

Families Citing this family (6)

* Cited by examiner, † Cited by third party
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CN104713972A (zh) * 2013-12-16 2015-06-17 上海宝锋工程技术有限公司 9-芴甲氧羰酰琥珀酰亚胺纯度的液相色谱测定方法
CN103630633A (zh) * 2013-12-20 2014-03-12 通化华夏药业有限责任公司 一种柱前衍生化法测定苦碟子注射液中6种氨基酸含量的方法
CN104155378B (zh) * 2014-08-14 2016-05-04 蚌埠丰原医药科技发展有限公司 一种快速分析氨基酸混合物的方法
CN104198643A (zh) * 2014-09-21 2014-12-10 上海海洋大学 一种同时测定三种芳香族氨基酸含量的方法
CN110133124A (zh) * 2019-04-29 2019-08-16 天津中医药大学 疏血通注射液中18种氨基酸的含量测定方法
CN111505160B (zh) * 2020-05-11 2021-04-06 成都市科隆化学品有限公司 一种Fmoc-保护氨基酸纯度及相关物质分析方法

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112578044A (zh) * 2020-12-02 2021-03-30 成都普康生物科技有限公司 一种高效液相检测 Fmoc-AEEA含量的方法
CN112578044B (zh) * 2020-12-02 2022-09-02 成都普康生物科技有限公司 一种高效液相检测Fmoc-AEEA含量的方法
CN114441681A (zh) * 2022-01-28 2022-05-06 山东省食品药品检验研究院 一种测定特殊医学配方食品中20种游离氨基酸和l-羟脯氨酸的方法
CN114441681B (zh) * 2022-01-28 2024-06-04 山东省食品药品检验研究院 一种测定特殊医学配方食品中20种游离氨基酸和l-羟脯氨酸的方法

Also Published As

Publication number Publication date
WO2012057598A1 (es) 2012-05-03

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