MX2010003369A - Regiones no traducidas 5' sinteticas, vectores de expresion y metodos para incrementar la expresion transgenica. - Google Patents

Regiones no traducidas 5' sinteticas, vectores de expresion y metodos para incrementar la expresion transgenica.

Info

Publication number
MX2010003369A
MX2010003369A MX2010003369A MX2010003369A MX2010003369A MX 2010003369 A MX2010003369 A MX 2010003369A MX 2010003369 A MX2010003369 A MX 2010003369A MX 2010003369 A MX2010003369 A MX 2010003369A MX 2010003369 A MX2010003369 A MX 2010003369A
Authority
MX
Mexico
Prior art keywords
utrs
synthetic
polynucleotide fragment
expression
methods
Prior art date
Application number
MX2010003369A
Other languages
English (en)
Inventor
Thomas Reed
Original Assignee
Intrexon Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Intrexon Corp filed Critical Intrexon Corp
Publication of MX2010003369A publication Critical patent/MX2010003369A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4732—Casein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0393—Animal model comprising a reporter system for screening tests
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription

Abstract

La presente invención proporciona 5'UTR (siglas en inglés para regiones no traducidas 5') sintéticas que comprenden un primer fragmento polinucleotídico y un segundo fragmento polinucleotídico, en donde el primer fragmento polinucleotídico comprende por lo menos un sitio de empalme de un primer gen eucariótico, el segundo fragmento polinucleotídico comprende por lo menos una porción de la región 5' no traducida de un segundo gen eucariótico y el primer fragmento polinucleotídico se localiza 5' respecto al segundo fragmento polinucleotídico. En una modalidad, el primer fragmento polinucleotídico comprende el segundo intrón de un gen para ATPasa de calcio sarcoplásmica/retículo endoplasmática y el segundo fragmento polinucleotídico comprende por lo menos una porción de la 5'UTR del gen para caseína eucariótica. Las 5'UTR sintéticas son útiles para incrementar la expresión de un transgén cuando se colocan entre un promotor y un transgén dentro de un vector de expresión. La presente invención también proporciona vectores que comprenden 5'UTR sintéticas y métodos para incrementar la expresión de un transgén utilizando 5'UTR sintéticas.
MX2010003369A 2007-09-26 2008-09-26 Regiones no traducidas 5' sinteticas, vectores de expresion y metodos para incrementar la expresion transgenica. MX2010003369A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US97540707P 2007-09-26 2007-09-26
PCT/US2008/078028 WO2009042971A2 (en) 2007-09-26 2008-09-26 Synthetic 5'utrs, expression vectors, and methods for increasing transgene expression

Publications (1)

Publication Number Publication Date
MX2010003369A true MX2010003369A (es) 2010-05-05

Family

ID=40512124

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2010003369A MX2010003369A (es) 2007-09-26 2008-09-26 Regiones no traducidas 5' sinteticas, vectores de expresion y metodos para incrementar la expresion transgenica.

Country Status (15)

Country Link
US (1) US8835621B2 (es)
EP (3) EP3156414B1 (es)
JP (1) JP5514727B2 (es)
KR (1) KR101541935B1 (es)
CN (1) CN101855233B (es)
AU (1) AU2008304201C1 (es)
BR (1) BRPI0817231B1 (es)
CA (1) CA2715078C (es)
DK (2) DK3156414T3 (es)
ES (1) ES2614402T3 (es)
HU (1) HUE031422T2 (es)
IL (1) IL204730A (es)
MX (1) MX2010003369A (es)
RU (1) RU2524431C2 (es)
WO (1) WO2009042971A2 (es)

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