MX168887B - Procedimiento para la produccion de polideoxirribonucleotido defibrotide - Google Patents

Procedimiento para la produccion de polideoxirribonucleotido defibrotide

Info

Publication number
MX168887B
MX168887B MX006108A MX610887A MX168887B MX 168887 B MX168887 B MX 168887B MX 006108 A MX006108 A MX 006108A MX 610887 A MX610887 A MX 610887A MX 168887 B MX168887 B MX 168887B
Authority
MX
Mexico
Prior art keywords
solution
polydeoxyribonucleotide
until
depolymerization
stranded
Prior art date
Application number
MX006108A
Other languages
English (en)
Inventor
Giusppe Prino
Gianfranco Fedeli
Giuseppe Diamantini
Marisa Mantovani
Original Assignee
Crinos Industria Farmaco
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Crinos Industria Farmaco filed Critical Crinos Industria Farmaco
Publication of MX168887B publication Critical patent/MX168887B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C07H1/08—Separation; Purification from natural products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/117—Nucleic acids having immunomodulatory properties, e.g. containing CpG-motifs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/17—Immunomodulatory nucleic acids

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención se refiere a procedimiento para la producción del polideoxirribonucleótido Defibrotide, a partir de una solución de ácido nucléicos de partida, cuya solución se encuentra al menos substancialmente libre de polisacáridos y proteínas, caracterizado porque comprende las etapas de: 1) formar polideoxirribonucleótidos altamente polimerizados e interrumpidos por agregación estabilizante de los ácidos nucléicos de partida, ajustar, si es necesario, la solución a una fuerza iónica predeterminada de por lo menos 1 molar y ajustar el pH de la solución hasta alcanzarse la máxima hipercromicidad reversible; 2) despolimerizar los polideoxirribonucleótidos hasta la hipercromicidad reversible de la solución se encuentra en un valor de h igual a 15 + (-) 5, medido como un porcentaje en DNA natural, por calentamiento de la solución resultante a una temperatura de despolimerización para dicho polideoxirribonucleótido y manteniendo la solución a una temperatura de despolimerización hasta conseguirse dicho valor de hipercromicidad reversible; 3) detener entonces la reacción de despolimerización y separar los enlaces hidrógeno de cualquier fragmento de doble filamento en la solución despolimerizada, para formar fragmentos polideoxirribonucleotídicos de un solo filamento; y 4) estabilizar posteriormente los fragmentos de polideoxirribonucleótidos de un solo filamento, resultantes, por calentamiento de la solución resultante a una temperatura y a un pH mayores que los correspondientes parámetros utilizados en la reación de despolimerización, para evitar la reformación de los enlaces hidrógeno.
MX006108A 1986-04-17 1987-04-20 Procedimiento para la produccion de polideoxirribonucleotido defibrotide MX168887B (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT20117/86A IT1190313B (it) 1986-04-17 1986-04-17 Procedimento per l'ottenimento di polidesossiribonucleotidi chimicamente definiti e riproducibili e prodotto farmacologicamente attivo risultante

Publications (1)

Publication Number Publication Date
MX168887B true MX168887B (es) 1993-06-14

Family

ID=11163944

Family Applications (1)

Application Number Title Priority Date Filing Date
MX006108A MX168887B (es) 1986-04-17 1987-04-20 Procedimiento para la produccion de polideoxirribonucleotido defibrotide

Country Status (20)

Country Link
US (1) US4985552A (es)
EP (1) EP0263155B1 (es)
JP (1) JP2581722B2 (es)
KR (1) KR880701242A (es)
CN (1) CN1018650B (es)
AR (1) AR242585A1 (es)
AT (1) ATE66927T1 (es)
AU (1) AU612976B2 (es)
BE (1) BE1002908A4 (es)
CA (1) CA1297430C (es)
CH (1) CH677114A5 (es)
DE (1) DE3772697D1 (es)
ES (1) ES2005168A6 (es)
FR (1) FR2597481B1 (es)
GR (1) GR870602B (es)
IL (1) IL82251A (es)
IT (1) IT1190313B (es)
MX (1) MX168887B (es)
WO (1) WO1987006235A1 (es)
ZA (1) ZA872755B (es)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5182269A (en) * 1987-09-23 1993-01-26 Crinos Industria Farmacobiologica Spa Composition for topical use having hair stimulating, anti-dandruff and anti-seborrhoic activity
IT1252174B (it) * 1991-12-09 1995-06-05 Crinos Industria Farmaco Oligodesossimibonucleotidi ad attivita' antiischemica e procedimenti per il loro ottenimento
IT1271685B (it) * 1994-08-03 1997-06-04 Crinos Industria Farmaco Impiego dei polidesossiribonucleotidi nelle neuropati diabetiche
US7026468B2 (en) 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US7807822B2 (en) 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
DK1059092T3 (da) * 1999-06-08 2006-03-27 Gentium Spa Anvendelse af komplekser af kationiske liposomer og polydeoxyribonukleotider som medikamenter
EP1147777A1 (en) 2000-04-18 2001-10-24 Crinos Industria Farmacobiologica S.p.A. Combination of defibrotide and G-CSF and its use to activate haematopoietic progenitors
US20020127587A1 (en) * 2001-02-13 2002-09-12 Domenica Simms Methods and compositions for isolation of biological macromolecules
EP1554398A4 (en) 2002-09-13 2005-12-14 Valentis Inc DEVICE AND METHOD FOR THE CLEANING OF NUCLEAR SULFUR IN THE PREPARATIVE SCALE
EP2216415B2 (en) * 2003-08-01 2017-01-04 Life Technologies Corporation Methods for preparing short RNA molecules
ITMI20031714A1 (it) * 2003-09-05 2005-03-06 Gentium Spa Formazioni ad azione antitumorale.
JP2008531647A (ja) * 2005-03-03 2008-08-14 ゲンチウム エスピーエー 抗腫瘍作用を有する製剤
EP2103689A1 (en) * 2008-03-19 2009-09-23 Gentium S.p.A. Synthetic phosphodiester oligonucleotides and therapeutical uses thereof
AU2010363814B2 (en) 2010-11-12 2016-05-19 Gentium S.R.L. Defibrotide for use in prophylaxis and/or treatment of Graft versus Host Disease (GVHD).
KR20150044877A (ko) * 2012-06-22 2015-04-27 젠티엄 에스피에이 디파이브로타이드의 생물학적 활성 측정을 위한 유우글로불린에 기초한 방법
EP3026122A1 (en) * 2014-11-27 2016-06-01 Gentium S.p.A. Cellular-based method for determining the potency of defibrotide
CN111132663A (zh) 2017-08-03 2020-05-08 爵士制药爱尔兰有限公司 包含高浓度核酸的制剂
MX2020010689A (es) 2018-04-12 2021-01-20 Jazz Pharmaceuticals Inc Defibrotida para la prevencion y tratamiento del sindrome de liberacion de citocinas y la neurotoxicidad asociada con la inmunodeplecion.
WO2019213053A1 (en) 2018-04-30 2019-11-07 Gentium S.R.L. Methods for treating patients having sinusoidal obstruction syndrome
US20220023533A1 (en) 2018-12-07 2022-01-27 Jazz Phrmaceticals Ireland Limited Subcutaneous delivery of high concentration formulations
EP4110287A1 (en) 2020-02-28 2023-01-04 Jazz Pharmaceuticals Ireland Limited Delivery of low viscosity formulations
US20230086833A1 (en) 2020-04-17 2023-03-23 Jazz Pharmaceuticals Ireland Limited Defibrotide treatment for the prevention of organ rejection and injury
WO2022234101A1 (en) 2021-05-06 2022-11-10 Jazz Pharmaceuticals Ireland Limited Defibrotide for the treatment and prevention of acute respiratory distress syndrome

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1371944A (fr) * 1963-07-30 1964-09-11 Centre Nat Rech Scient Procédé de préparation d'acides désoxyribonucléiques hautement polymérisés
DE2154279A1 (de) * 1970-11-03 1972-05-25 Crinos Industria Farmaco Medikamente für das fibrinolytische System
IT1043823B (it) * 1970-11-03 1980-02-29 Prephar Procedimento per l estrazione di acidi nucleici da organi animali
US3899481A (en) * 1970-11-03 1975-08-12 Crinos Industria Farmaco Process for the controlled partial degradation of deoxyribonucleic acid extracted from animal organs
CH617963A5 (es) * 1973-10-05 1980-06-30 Derivati Organici Lab
SU652187A1 (ru) * 1975-07-23 1979-03-15 Киевский Ордена Ленина Государственный Университет Им. Т.Г.Шевченко Способ получени дезоксирибонуклеиновой кислоты
JPS554308A (en) * 1978-06-23 1980-01-12 Yuki Gosei Yakuhin Kogyo Kk Isolation of deoxyribonucleic acid from milt of fish
IT1170215B (it) * 1983-09-12 1987-06-03 Crinos Industria Farmaco Composizione farmaceutica per il trattamento di stati di insufficienza renale acuta
IT1206341B (it) * 1984-02-16 1989-04-14 Crinos Industria Farmaco Composizione farmaceutica per il trattamento dell'ischemia acuta del miocardio.

Also Published As

Publication number Publication date
AU612976B2 (en) 1991-07-25
CH677114A5 (es) 1991-04-15
CN1018650B (zh) 1992-10-14
IL82251A0 (en) 1987-10-30
BE1002908A4 (fr) 1991-07-30
GR870602B (en) 1987-08-17
CA1297430C (en) 1992-03-17
ZA872755B (en) 1987-10-05
AR242585A1 (es) 1993-04-30
FR2597481B1 (fr) 1990-01-12
JP2581722B2 (ja) 1997-02-12
CN87103688A (zh) 1988-01-20
IT1190313B (it) 1988-02-16
IT8620117A0 (it) 1986-04-17
AU7288687A (en) 1987-11-09
IL82251A (en) 1991-05-12
ES2005168A6 (es) 1989-03-01
US4985552A (en) 1991-01-15
KR880701242A (ko) 1988-07-26
WO1987006235A1 (en) 1987-10-22
JPS63503067A (ja) 1988-11-10
EP0263155B1 (en) 1991-09-04
FR2597481A1 (fr) 1987-10-23
EP0263155A1 (en) 1988-04-13
DE3772697D1 (de) 1991-10-10
IT8620117A1 (it) 1987-10-17
ATE66927T1 (de) 1991-09-15

Similar Documents

Publication Publication Date Title
MX168887B (es) Procedimiento para la produccion de polideoxirribonucleotido defibrotide
US2426125A (en) Manufacture of glycol alginates
ES2057602T3 (es) Procedimiento para la obtencion hidrotermica de disilicato sodico cristalino.
KR900701808A (ko) 알킬 글리코시드의 직접 제조방법
JP2009521921A5 (es)
Melki et al. Oscillations in microtubule polymerization: the rate of GTP regeneration on tubulin controls the period.
PL320367A1 (en) Caprolactam producing method
ATE58918T1 (de) Verfahren zur herstellung von kristalliner larabinose.
BRPI9914947B8 (pt) processo de polimerização por aquecimento dielétrico de ácidos graxos, ésteres de ácidos graxos, hidrocarbonetos insaturados, ou derivados insaturados destes produtos e produtos assim obtidos e seus usos
ES2070335T3 (es) Procedimiento para la produccion de acido hialuronico de bajo peso molecular.
HUP9802529A2 (hu) Eljárás kalcium-borát előállítására
GR890100070A (el) Μέ?οδος παρασκευής Ν-φωσφονό-με?υλό-ιμινό-διοξικού οξέος.
Szewczak et al. On the conformation of the alpha sarcin stem-loop of 28S rRNA
EP1115654A1 (en) Production of calcium hydrogenphosphate or monocalcium phosphate from calcium phosphate
PL285638A1 (en) Method of obtaining alkylosulfonic acids
SU580824A3 (ru) Способ получени фосфата кали
ATE101103T1 (de) Verfahren zur herstellung von ammoniumpolyphosphat, welches eine niedrigviskose waessrige suspension ergibt.
US852371A (en) Method of producing dicalcium phosphate.
SU765208A1 (ru) Способ получени перекиси бари
SU1710502A1 (ru) Способ получени гидрофосфата меди
SU405359A1 (ru) Способ получени 6-диэтиленимидофосфамидо-2-диметиламино-7-метилпурина
SU382623A1 (ru) Способ получения 2,2-диалкил-4-арил- 5,6-бензизоиндолинов
US3369017A (en) Preparation of decarbonylation products of carboxylic acids
Symons Possible mechanisms for strand-breaks in DNA induced by stirring
SU1178432A1 (ru) Способ устранени деформации стопы