MD2969F1 - Procedeu de cultivare a bacteriilor lactice (variante) - Google Patents
Procedeu de cultivare a bacteriilor lactice (variante) Download PDFInfo
- Publication number
- MD2969F1 MD2969F1 MDA20040245A MD20040245A MD2969F1 MD 2969 F1 MD2969 F1 MD 2969F1 MD A20040245 A MDA20040245 A MD A20040245A MD 20040245 A MD20040245 A MD 20040245A MD 2969 F1 MD2969 F1 MD 2969F1
- Authority
- MD
- Moldova
- Prior art keywords
- lactic
- culture medium
- cultivation
- milk
- bacteria
- Prior art date
Links
- 241000894006 Bacteria Species 0.000 title abstract 8
- 238000000034 method Methods 0.000 title abstract 5
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 abstract 10
- 239000001963 growth medium Substances 0.000 abstract 8
- 239000004310 lactic acid Substances 0.000 abstract 5
- 235000014655 lactic acid Nutrition 0.000 abstract 5
- 238000000926 separation method Methods 0.000 abstract 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 abstract 3
- 239000008346 aqueous phase Substances 0.000 abstract 3
- 239000011159 matrix material Substances 0.000 abstract 3
- 235000013336 milk Nutrition 0.000 abstract 3
- 239000008267 milk Substances 0.000 abstract 3
- 210000004080 milk Anatomy 0.000 abstract 3
- 239000003795 chemical substances by application Substances 0.000 abstract 2
- 238000000605 extraction Methods 0.000 abstract 2
- 238000007654 immersion Methods 0.000 abstract 2
- 230000008929 regeneration Effects 0.000 abstract 2
- 238000011069 regeneration method Methods 0.000 abstract 2
- 238000009331 sowing Methods 0.000 abstract 2
- 239000002253 acid Substances 0.000 abstract 1
- 239000003957 anion exchange resin Substances 0.000 abstract 1
- 150000001450 anions Chemical class 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 235000020247 cow milk Nutrition 0.000 abstract 1
- 238000010828 elution Methods 0.000 abstract 1
- 235000013305 food Nutrition 0.000 abstract 1
- 239000002609 medium Substances 0.000 abstract 1
- 238000006386 neutralization reaction Methods 0.000 abstract 1
- 235000008935 nutritious Nutrition 0.000 abstract 1
- 235000020200 pasteurised milk Nutrition 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 239000011347 resin Substances 0.000 abstract 1
- 229920005989 resin Polymers 0.000 abstract 1
- 239000007787 solid Substances 0.000 abstract 1
- 238000003756 stirring Methods 0.000 abstract 1
- 235000013618 yogurt Nutrition 0.000 abstract 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Dairy Products (AREA)
Abstract
Inventia se refera la industria laptelui, in particular la un procedeu de cultivare a bacteriilor lactice. Procedeul de cultivare a bacteriilor lactice include pregatirea mediului de cultura, care contine lapte sterilizat si racit pana la temperatura de insamantare, adaugarea in mediul pregatit a culturii de bacterii lactice, cultivarea si separarea lor. Pentru insamantare se utilizeaza bacterii lactice, crescute pe un mediu nutritiv ce consta din iaurt:lapte pasteurizat sau din lapte batut:lapte de vaca proaspat pasteurizat, luate in raportul de volum de 1:10, si liofilizate, care se adauga in mediul de cultura prin agitare la temperatura constanta, dupa aceea, conform primei variante, mediul de cultura se imobilizeaza intr-o matrice poroasa, cultivarea bacteriilor lactice se efectueaza la temperatura de 36...40°C timp de 2...3 ore cu eliminarea acidului lactic format prin elutie continua in faza apoasa mobila, care se separa de matricea cu mediul de cultura imobilizat si dupa separarea acidului lactic de faza apoasa se returneaza in mediul de cultura. Procedeul de cultivare a bacteriilor lactice conform variantei a doua include introducerea in mediul de cultura a unui agent bazic cu grad de puritate alimentar, insolubil in el, care in perioada cultivarii fixeaza continuu acidul lactic format in mediul de cultura cu realizarea continua a ciclului de introducere, evacuare si regenerare a agentului bazic si de extractie si separare a acidului lactic. Procedeul de cultivare a bacteriilor lactice conform variantei a treia include imersarea in mediul de cultura a unei matrice solide insolubile din rasini schimbatoare de anioni, completate cu grupari OH- schimbabile, de tipul R+OH-, cu care, in perioada de cultivare, prin realizarea continua a ciclului de imersare, evacuare si regenerare a rasinii intr-o solutie de hidroxid de sodiu, se fixeaza continuu anionul lactic, iar cu gruparea OH- libera se realizeaza neutralizarea concomitenta a protonului cu formarea fazei apoase, care se separa de mediul de cultura cu extragerea si separarea ulterioara a acidului lactic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20040245A MD2969G2 (ro) | 2004-10-04 | 2004-10-04 | Procedeu de cultivare a bacteriilor lactice (variante) |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20040245A MD2969G2 (ro) | 2004-10-04 | 2004-10-04 | Procedeu de cultivare a bacteriilor lactice (variante) |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD2969F1 true MD2969F1 (ro) | 2006-02-28 |
| MD2969G2 MD2969G2 (ro) | 2006-09-30 |
Family
ID=36002216
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDA20040245A MD2969G2 (ro) | 2004-10-04 | 2004-10-04 | Procedeu de cultivare a bacteriilor lactice (variante) |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD2969G2 (ro) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD145Z (ro) * | 2009-07-22 | 2010-09-30 | Институт Физиологии И Санокреатологии Академии Наук Молдовы | Procedeu de obţinere a mediului nutritiv pentru cultivarea lactobacteriilor |
| MD190Z (ro) * | 2009-10-19 | 2010-12-31 | Общественное Учреждение Научно-Практический Институт Садоводства И Пищевых Технологий | Procedeu de obţinere a maielei bacteriene pentru produse lactate acide combinate |
-
2004
- 2004-10-04 MD MDA20040245A patent/MD2969G2/ro not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| MD2969G2 (ro) | 2006-09-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG4A | Patent for invention issued | ||
| KA4A | Patent for invention lapsed due to non-payment of fees (with right of restoration) | ||
| MM4A | Patent for invention definitely lapsed due to non-payment of fees |