MD1880Z

MD1880Z - Process for producing royal jelly

Info

Publication number: MD1880Z
Application number: MDS20250022A
Authority: MD
Inventors: Николае ЕРЕМИЯ; Флюр МАКАЕВ; Виталие ЖЕРЕГИ; Ольга КОШЕЛЕВА; Сергей ПОГРЕБНОЙ; Татьяна МАРДАРЬ; Сюзанна МОДВАЛА; Наталия СУКМАН; Яна ПЕТКУ
Original assignee: Публичное Учреждение Технический Университет Молдовы; Публичное Учреждение Государственный Университет Молдовы
Priority date: 2025-03-07
Filing date: 2025-03-07
Publication date: 2026-01-31
Also published as: MD1880Y

Abstract

Изобретение относится к пчеловодству, в частности к способу получения маточного молочка.Способ согласно изобретению включает введение в пчелиные семьи 1...3 стандартных рамок с элементами Никота с 40...110 трансвазационными личинками и кормление пчел-кормилиц, при отсутствии поддерживающего медосбора, смесью сахарного сиропа в концентрации 50% с биостимулятором в дозах 1,0...2,0 мл/л, в количестве 1,0 л смеси при введении рамок с трансвазационными личинками в течение 3 суток, после чего рамки вынимают из улья для сбора молочка, затем процедуру повторяют дважды, при этом биостимулятор представляет 3% водный раствор хлорида холина.Результатом изобретения состоит в увеличении числа принятых личинок на выращивание, диаметре, длине и массе маточников, улучшении физиологического состояния пчел, стимуляции секреции и увеличении продукции маточного молочка с одного маточника, так и от семьи в целом.The invention relates to beekeeping, in particular to a method for obtaining royal jelly. The method according to the invention includes introducing 1...3 standard frames with Nicot elements with 40...110 transvasation larvae into bee colonies and feeding nurse bees, in the absence of a supporting honey flow, with a mixture of sugar syrup at a concentration of 50% with a biostimulant in doses of 1.0...2.0 ml / l, in the amount of 1.0 l of the mixture when introducing frames with transvasation larvae for 3 days, after which the frames are removed from the hive to collect royal jelly, then the procedure is repeated twice, while the biostimulant is a 3% aqueous solution of choline chloride. The result of the invention is an increase in the number of larvae accepted for rearing, the diameter, length and weight of queen cells, an improvement in the physiological state of bees, stimulation of secretion and an increase in the production of royal jelly from one queen cell, and from a family in in general.

Description

[0001] Lăptişorul de matcă este o secreţie a glandelor hipofaringiene şi mandibulare ale albinelor tinere (doici) la vârsta de 3-12 zile, care este folosită pentru hrănirea larvelor tinere în primele trei zile, a larvelor de matcă pe toată perioada larvară şi a mătcilor. Glandele hipofaringiene încep a funcţiona la a 5-6-a zi, iar dezvoltarea maximă o ating la albinele în vârsta de 10-12 zile. Începând cu a 11-12-a zi de viaţă a albinelor încetează treptat alimentaţia lor cu polen, iar la a 14-15-a zi şi secreţia lăptişorului [Eremia N. Apicultura. Chişinău, 2020, p. 311, alin. 1-4].[0001] Royal jelly is a secretion of the hypopharyngeal and mandibular glands of young bees (nurses) at the age of 3-12 days, which is used to feed young larvae in the first three days, queen larvae throughout the larval period and queens. The hypopharyngeal glands begin to function on the 5-6th day, and they reach their maximum development in bees at the age of 10-12 days. Starting from the 11-12th day of life, the bees gradually stop feeding on pollen, and on the 14-15th day, the secretion of royal jelly also stops [Eremia N. Apicultura. Chişinău, 2020, p. 311, para. 1-4].

[0002] Este cunoscut procedeul de hrănire a albinelor, în care în calitate de înlocuitori alimierii se foloseşte zahărul. Pentru stimularea creşterii puietului în perioada de primăvară se utilizează sirop de zahăr de 50% (1kg de zahăr la un litru de apă) [1].[0002] The process of feeding bees is known, in which sugar is used as a food substitute. To stimulate the growth of the brood in the spring period, 50% sugar syrup is used (1 kg of sugar per liter of water) [1].

[0003] Dezavantajul acestui procedeu constă în uzarea albinelor şi reducerea longevităţii lor.The disadvantage of this process is that it wears out the bees and reduces their longevity.

[0004] Este cunoscută compoziţia nutritivă pentru hrănirea albinelor, care conţine hidrolizat alcalin al polenului şi sirop de zahăr de 50% [2].[0004] A nutritional composition for feeding bees is known, which contains alkaline pollen hydrolysate and 50% sugar syrup [2].

[0005] Dezavantajul acestei compoziţii constă în aceea că hrana utilizată nu contribuie suficient la ameliorarea stării fiziologice a albinelor.[0005] The disadvantage of this composition is that the food used does not contribute sufficiently to improving the physiological condition of the bees.

[0006] Este cunoscută compoziţia nutritivă pentru hrănirea albinelor, care conţine sirop de zahăr de 50%, polen şi ulei eteric de izmă [3].[0006] The nutritional composition for feeding bees is known, which contains 50% sugar syrup, pollen and essential oil of isma [3].

[0007] Dezavantajul acestei compoziţii constă în aceea că hrana utilizată nu este suficient de nutritiva.[0007] The disadvantage of this composition is that the food used is not sufficiently nutritious.

[0008] Este cunoscut procedeul de creştere a mătcilor, în care se utilizează sistemul Nicot cu unele elemente care cuprind: căpăcel de fixare pentru portcupulă, portcupulă şi cupulă, care se pot folosi şi la obţinerea lăptişorului de matcă [4].[0008] The process of raising queen bees is known, in which the Nicot system is used with some elements that include: a fixing cap for the cup holder, the cup holder and the cup, which can also be used to obtain royal jelly [4].

[0009] Este cunoscut procedeul de hrănire a albinelor, în perioada lipsei culesului melifer, care recomandă stimularea zilnică a familiilor doici cu sirop de zahăr şi cu polen sau substituienţi [5].[0009] The process of feeding bees during the period of lack of honey collection is known, which recommends daily stimulation of nurse colonies with sugar syrup and pollen or substitutes [5].

[0010] Dezavantajul acestui procedeu constă în uzarea albinelor, reducerea longevităţii lor şi productivităţii familiilor de albine.[0010] The disadvantage of this process is the wear and tear on the bees, reducing their longevity and the productivity of the bee colonies.

[0011] Problema pe care o rezolvă invenţia constă în elaborarea unui procedeu de obţinere a lăptişorului de matcă cu ameliorarea stării fiziologice a albinelor doici, sporirea numărului de larve acceptate la creştere, stimularea secretării şi producerii lăptişorului de matcă, când în natură lipseşte culesul melifer.[0011] The problem solved by the invention consists in developing a process for obtaining royal jelly with the improvement of the physiological state of nurse bees, increasing the number of larvae accepted for growth, stimulating the secretion and production of royal jelly, when in nature there is no honey collection.

[0012] Problema se soluţionează prin aceea că a fost elaborat un procedeu de obţinere a lăptişorului de matcă, care include introducerea în familiile crescătoare a 1-3 rame standard de 435x300 mm de două tipuri:[0012] The problem is solved by developing a process for obtaining royal jelly, which includes introducing 1-3 standard 435x300 mm frames of two types into the breeding families:

[0013] • rame cu 2 şipci (la nivelul superior şi de mijloc) echipate cu 30 botce (15/15);[0013] • 2-slat frames (at the upper and middle level) equipped with 30 bolts (15/15);

[0014] • rame cu 3 şipci (la nivelul superior, de mijloc şi inferior) echipate cu 40 botce (14/13/13), cu 40-110 larve transvazate şi hrănirea zilnică a albinelor doici, în lipsa unui cules melifer de întreţinere, cu un amestec din sirop de zahăr, în concentraţie de 50% şi un biostimulator în doză de 1,0-2,0 ml/L, în cantitate de 1,0 L de amestec la o familie, timp de 3 zile, după care ramele sunt scoase din stup pentru colectarea lăptişorului, iar procedura se mai repetă de două ori.[0014] • frames with 3 slats (at the upper, middle and lower levels) equipped with 40 combs (14/13/13), with 40-110 transferred larvae and daily feeding of the nurse bees, in the absence of a maintenance honey collection, with a mixture of sugar syrup, in a concentration of 50% and a biostimulator in a dose of 1.0-2.0 ml/L, in an amount of 1.0 L of mixture per family, for 3 days, after which the frames are removed from the hive to collect the royal jelly, and the procedure is repeated twice more.

[0015] Totodată botcele constau din căpăcel de fixare pentru portcupulă, portcupulă şi cupulă. Biostimulatorul utilizat, reprezintă o soluţie apoasă de clorură de colină de 3%.[0015] At the same time, the botcells consist of a fixing cap for the cup holder, the cup holder and the cup. The biostimulator used is a 3% aqueous solution of choline chloride.

[0016] Rezultatul invenţiei constă în sporirea numărului de larve acceptate la creştere, îmbunătăţirea stării fiziologice a albinelor, stimularea secretării şi sporirea producerii lăptişorului de matcă atât dintr-o botcă, cât şi de la o familie la o repriză.[0016] The result of the invention consists in increasing the number of larvae accepted for rearing, improving the physiological state of the bees, stimulating the secretion and increasing the production of royal jelly both from a hive and from a family in one round.

[0017] Exemplu de realizare a invenţiei.[0017] Example of embodiment of the invention.

[0018] Pentru efectuarea experienţei au fost formate patru loturi de familii de albine, după principiile metodelor de analogie, după numărul de faguri, puterea familiei, numărul fagurilor cu puiet căpăcit şi rezerva de miere în stup.[0018] To carry out the experiment, four batches of bee families were formed, according to the principles of analogy methods, according to the number of combs, the strength of the family, the number of combs with brood capped and the honey reserve in the hive.

[0019] În lipsa culesului melifer de întreţinere, familiilor de albine din loturile experimentale li s-a administrat câte 1 litru de amestec de sirop de zahăr de 50% cu biostimulatorul - clorura de colină: lotul I - cu 1,0 ml/L, lotul II - cu 1,5 ml/L, lotul III - cu 2,0 ml/L, iar lotul IV (martor) - sirop de zahăr pur.[0019] In the absence of maintenance honey collection, bee colonies in the experimental batches were administered 1 liter of a mixture of 50% sugar syrup with the biostimulator - choline chloride: batch I - with 1.0 ml/L, batch II - with 1.5 ml/L, batch III - with 2.0 ml/L, and batch IV (control) - pure sugar syrup.

[0020] La prima repriză în familiile doici (crescătoare) a fost introduse două rame cu 70 larve in total (prima cu 40 şi a doua cu 30 larve), la a doua repriză au fost introduse trei rame cu un total de 110 larve (două a câte 40 larve şi a treia cu 30 larve), la a treia repriză - o ramă cu 40 larve.[0020] In the first half, two frames with a total of 70 larvae were introduced into the nurse (breeding) families (the first with 40 and the second with 30 larvae), in the second half, three frames with a total of 110 larvae were introduced (two with 40 larvae and the third with 30 larvae), in the third half - one frame with 40 larvae.

[0021] Albinelor doici li s-a administrat câte 1 litru de amestec de sirop de zahăr de 50% şi biostimulator, 3 zile la rând de la introducerea ramelor cu larve transvazate, după care ramele cu botce s-au scos din stup pentru colectarea lăptişorului de matcă şi s-a repetat a doua, apoi a treia repriză.[0021] The nurse bees were administered 1 liter of a mixture of 50% sugar syrup and biostimulator, 3 days in a row after the introduction of the frames with transferred larvae, after which the frames with the bees were removed from the hive to collect the royal jelly and the second and then the third round was repeated.

[0022] În timpul efectuării experienţelor s-au studiat următorii indici: procentul de acceptare a larvelor la creştere, diametrul, lungimea şi masa botcelor, cantitatea de lăptişor de matcă obţinut dintr-o botcă şi de la o familie doică la o repriză.[0022] During the experiments, the following indices were studied: the percentage of larvae acceptance for growth, the diameter, length and mass of the brood, the amount of royal jelly obtained from a brood and from a nurse family per session.

[0023] Familiile de albine din experienţă aveau câte 18 faguri, puterea familiilor - 16 ulicioare (spaţii dintre fagurii ocupaţi cu albine), rezerva de miere 14,0 kg şi 4-5 faguri cu puiet căpăcit. În prima repriză familiilor doici li s-a dat la creştere 2 rame cu 70 de larve transvazate (40+30 buc.) cu vârsta 10-12 ore.[0023] The bee colonies in the experiment had 18 combs each, the strength of the colonies - 16 alleys (spaces between the combs occupied by bees), the honey reserve was 14.0 kg and 4-5 combs with capped brood. In the first half, the nurse colonies were given 2 frames with 70 transferred larvae (40+30 pcs.) aged 10-12 hours for rearing.

[0024] Rezultatele cercetărilor efectuate au arătat că familiile crescătoare din loturile experimentale în prima repriză unde au fost date la creştere câte 70 de larve transvazate au fost acceptate 42-51 larve sau cu 20,0-32,9% mai mult faţă de lotul IV martor (tabelul 1).[0024] The results of the research showed that the breeding families from the experimental batches in the first half where 70 transfused larvae were given for breeding accepted 42-51 larvae or 20.0-32.9% more than the control batch IV (table 1).

[0025] Tabelul 1[0025] Table 1

[0026] Numărul de larve transvazate şi acceptate la creştere,[0026] Number of larvae transferred and accepted for rearing,

[0027] 14-17.07.2024 (I-a transvazare - 70 larve)[0027] 14-17.07.2024 (1st transfer - 70 larvae)

Lotullot

Nr. fag., buc.No. of sheets, pcs.

Puterea f/a, ulicioarePower f/a, alley

Rezerva de miere, kgHoney reserve, kg

Nr. fag. cu puiet căpăcit, buc.No. of beeches with hatched chicks, pcs.

Nr. larvelor transvazate, buc.No. of transferred larvae, pcs.

Nr. larvelor acceptateNo. of larvae accepted

buc.pcs.

%%

I - Sirop de zahăr + Clorură de colină de 3%, 1,0 ml/lI - Sugar syrup + 3% choline chloride, 1.0 ml/l

1818

1616

14,014.0

55

7070

5151

72,972.9

II - Sirop de zahăr + Clorură de colină de 3%, 1,5 ml/lII - Sugar syrup + 3% choline chloride, 1.5 ml/l

1818

1616

14,014.0

44

7070

4242

60,060.0

III - Sirop de zahăr + Clorură de colină de 3%, 2,0 ml/lIII - Sugar syrup + 3% choline chloride, 2.0 ml/l

1818

1616

14,014.0

55

7070

4343

61,461.4

IV - Sirop de zahăr pur (martor)IV - Pure sugar syrup (control)

1818

1616

14,014.0

55

7070

2828

40,040.0

[0029] S-a stabilit că diametrul botcelor din loturile experimentale constituie în medie 9,44-10,65 mm, lungimea botcelor - 20,82-24,47 mm. Cel mai mare diametru a fost la botcele din lotul III- 10,65 mm sau cu 4,7% faţă de lotul IV (martor), diferenţa fiind autentică (**B2 ≥ 0,99), lungimea botcelor la lotul II a fost de 24,47 mm sau cu 9,3% faţă de lotul martor (*B1 ≥ 0,95).[0029] It was established that the diameter of the bolls in the experimental batches is on average 9.44-10.65 mm, the length of the bolls - 20.82-24.47 mm. The largest diameter was in the bolls of batch III - 10.65 mm or 4.7% compared to batch IV (control), the difference being authentic (**B2 ≥ 0.99), the length of the bolls in batch II was 24.47 mm or 9.3% compared to the control batch (*B1 ≥ 0.95).

[0030] Masa botcelor cu larve şi lăptişor la loturile experimentale a fost în medie de 0,965 - 1,012 g sau cu 2,5-7,5% mai mare faţă de lotul martor (*B1 ≥ 0,95) (tabelul 2).[0030] The mass of the pods with larvae and milkweed in the experimental groups was on average 0.965 - 1.012 g or 2.5-7.5% higher than the control group (*B1 ≥ 0.95) (table 2).

[0031] Tabelul 2[0031] Table 2

[0032] Indicii botcelor şi cantitatea de lăptişor de matcă obţinut,[0032] The indices of the beehives and the amount of royal jelly obtained,

[0033] 14-17.07.2024 (I-a transvazare -70 larve)[0033] 14-17.07.2024 (1st transfer - 70 larvae)

Lotullot

IndiciiIndices

Diametrul botcelor, mmDiameter of the barrels, mm

Lungimea botcelor, mmLength of the boots, mm

Masa botcei cu larvă şi lăptişor, gMass of the bot with larva and milkweed, g

Cantitatea lăptişorului obţinut dintr-o botcă, gThe amount of milk obtained from one barrel, g

Cantitatea totală de lăptişor obţinut, gTotal amount of milk obtained, g

I-Sirop de zahăr + Clorură de colină de 3%, 1,0 ml/lI-Sugar syrup + 3% choline chloride, 1.0 ml/l

±±

9,44±0,0809.44±0.080

20,82±0,24520.82±0.245

1,00±0,014*1.00±0.014*

0,351±0,008***0.351±0.008***

17,90117,901

V, %V, %

5,645.64

7,737.73

9,259.25

14,3014.30

--

II-Sirop de zahăr + Clorură de colină de 3%, 1,5 ml/lII-Sugar syrup + 3% choline chloride, 1.5 ml/l

±±

10,14±0,09010.14±0.090

24,47±0,268*24.47±0.268*

0,965±0,0190.965±0.019

0,334±0,017***0.334±0.017***

14,02814,028

V, %V, %

5,845.84

8,198.19

13,0713.07

32,8032.80

--

III-Sirop de zahăr + Clorură de colină de 3%, 2,0 ml/lIII-Sugar syrup + 3% choline chloride, 2.0 ml/l

±±

10,65±0,085**10.65±0.085**

23,47±0,27423.47±0.274

1,012±0,015*1.012±0.015*

0,305±0,009***0.305±0.009***

13,11513,115

V, %V, %

5,245.24

7,667.66

9,639.63

19,1019.10

--

IV-Sirop de zahăr pur (martor)IV-Pure sugar syrup (control)

±±

10,17±0,14610.17±0.146

22,39±0,51322.39±0.513

0,941±0,0220.941±0.022

0,211±0,0140.211±0.014

5,9085,908

V, %V, %

7,527.52

12,1112.11

12,1612.16

35,5235.52

--

[0035] **Diametrul botcelor: lotul III / lotul IV - **B2 ≥ 0,99[0035] **Bottle diameter: batch III / batch IV - **B2 ≥ 0.99

[0036] * Lungimea botcelor: lotul II / lotul IV - *B1 ≥ 0,95[0036] * Length of the boots: batch II / batch IV - *B1 ≥ 0.95

[0037] *Masa botcei cu larvă şi lăptişor: lotul I / lotul IV - *B1 ≥ 0,95; lotul III / lotul IV - *B1 ≥ 0,95[0037] *Weight of the cocoon with larva and milkweed: batch I / batch IV - *B1 ≥ 0.95; batch III / batch IV - *B1 ≥ 0.95

[0038] *** Cantitatea lăptişorului obţinut dintr-o botcă: lotul I / lotul IV - ***B3 ≥ 0,999;[0038] *** Quantity of milk obtained from a barrel: batch I / batch IV - ***B3 ≥ 0.999;

[0039] lotul II / lotul IV - *B1 ≥ 0,95; lotul III / lotul IV - *B1 ≥ 0,95.[0039] batch II / batch IV - *B1 ≥ 0.95; batch III / batch IV - *B1 ≥ 0.95.

[0040] Dintr-o botcă în loturile experimentale s-au obţinut în medie câte 0,305-0,351 g de lăptişor de matcă sau cu 0,094 - 0,140 g (44,5-66,3%) mai mult decât lotul martor (***B3 ≥ 0,999). Cea mai mare cantitate de lăptişor de matcă s-a obţinut la lotul I - 17,901 g sau cu 11,99 g mai mult decât la lotul martor (303%).[0040] From one beehive in the experimental groups, an average of 0.305-0.351 g of royal jelly was obtained, or 0.094 - 0.140 g (44.5-66.3%) more than in the control group (***B3 ≥ 0.999). The largest amount of royal jelly was obtained in group I - 17.901 g, or 11.99 g more than in the control group (303%).

[0041] Coeficientul de variaţie a indicilor studiaţi a oscilat între 5,24 (diametrul botcelor) şi 35,52 (cantitatea lăptişorului obţinut dintr-o botcă).[0041] The coefficient of variation of the studied indices ranged between 5.24 (diameter of the pods) and 35.52 (amount of milk obtained from a pod).

[0042] Aşadar, în prima repriză când s-au dat la creştere 2 rame cu 70 larve (30+40 buc.), hrănirea albinelor doici a stimulat acceptarea larvelor cu 20,0-32,9%, dezvoltarea botcelor (diametrul cu 4,7%, lungimea cu 4,8-9,3%, masa botcelor cu larvă şi lăptişor cu 2,5-7,5%), cantitatea de lăptişor de matcă dintr-o botcă cu 44,5-66,3%, iar cantitatea totală - cu 307,5%.[0042] Therefore, in the first half when 2 frames with 70 larvae (30+40 pcs.) were raised, feeding the nurse bees stimulated the acceptance of the larvae by 20.0-32.9%, the development of the pods (diameter by 4.7%, length by 4.8-9.3%, mass of the pods with larva and royal jelly by 2.5-7.5%), the amount of royal jelly in a pod by 44.5-66.3%, and the total amount - by 307.5%.

[0043] În a doua repriză, în familiile experimentale au fost introduse câte trei rame (prima şi a doua cu câte 40 larve, a treia - cu 30 larve), în total - 110 larve transvazate.[0043] In the second half, three frames were introduced into the experimental families (the first and second with 40 larvae each, the third - with 30 larvae), in total - 110 transferred larvae.

[0044] Rezultatele au demonstrat că au fost acceptate 35-49 larve sau 31,8-44,5% din numărul total. Familiile crescătoare din loturile experimentale au acceptat cu 23-37 buc. sau cu 20,9-33,6% mai multe larve faţă de lotul martor (tabelul 3).[0044] The results showed that 35-49 larvae or 31.8-44.5% of the total number were accepted. The breeding families in the experimental groups accepted 23-37 pcs. or 20.9-33.6% more larvae than the control group (table 3).

[0045] Tabelul 3[0045] Table 3

[0046] Numărul de larve transvazate şi acceptate la creştere,[0046] Number of larvae transferred and accepted for rearing,

[0047] 17-20.07.2024 (a II-a transvazare)[0047] 17-20.07.2024 (2nd transfer)

Lotullot

Nr. larvelor transvazate, buc.No. of transferred larvae, pcs.

Nr. larvelor acceptateNo. of larvae accepted

buc.pcs.

%%

110110

4949

44,544.5

110110

3636

32,732.7

II - Sirop de zahăr + Clorură de colină de 3%, 2,0 ml/lII - Sugar syrup + 3% choline chloride, 2.0 ml/l

110110

3535

31,831.8

IV - Sirop de zahăr pur (martor)IV - Pure sugar syrup (control)

110110

1212

10,910.9

[0049] Diametrul botcelor din loturile experimentale a variat, în medie, între 8,71 şi 10,18 mm sau cu 5,7-12,4% mai mare ca în lotul martor, lungimea - 20,47-22,71 mm (2,0-13,1%) şi masa botcelor cu larvă şi lăptişor - 0,904-1,029 g (2,3-16,4%).[0049] The diameter of the bolls in the experimental batches varied, on average, between 8.71 and 10.18 mm or 5.7-12.4% higher than in the control batch, the length - 20.47-22.71 mm (2.0-13.1%) and the mass of bolls with larva and milkweed - 0.904-1.029 g (2.3-16.4%).

[0050] Cantitatea de lăptişor de matcă obţinut dintr-o botcă la loturile experimentale a constituit în medie 0,268-0,301 g sau cu 4,3-17,1% mai mult faţă de lotul martor, iar cantitatea totală - 9,648-13,818 g sau cu 6,564-10,734 g mai mult faţă de lotul martor (tabelul 4). Coeficientul de variaţie a oscilat între 7,34 (diametrul botcelor) şi 29,51% (cantitatea de lăptişor de matcă obţinut dintr-o botcă).[0050] The amount of royal jelly obtained from one beehive in the experimental groups was on average 0.268-0.301 g or 4.3-17.1% more than in the control group, and the total amount - 9.648-13.818 g or 6.564-10.734 g more than in the control group (table 4). The coefficient of variation ranged between 7.34 (beehive diameter) and 29.51% (amount of royal jelly obtained from one beehive).

[0051] Tabelul 4[0051] Table 4

[0052] Indicii botcelor şi cantitatea de lăptişor de matcă obţinut,[0052] The indices of the beehives and the amount of royal jelly obtained,

[0053] 17-20.07.2024 (a II-a transvazare - 110 larve)[0053] 17-20.07.2024 (2nd transfer - 110 larvae)

Lotullot

IndiciiIndices

Diametrul botcelor, mmDiameter of the barrels, mm

Lungimea botcelor, mmLength of the boots, mm

Cantitate totală de lăptişor obţinut, gTotal amount of milk obtained, g

±±

8,71±0,0918.71±0.091

21,06±0,48821.06±0.488

0,946±0,0200.946±0.020

0,282±0,0080.282±0.008

13,81813,818

V, %V, %

7,347.34

16,2216.22

15,1815.18

20,9420.94

--

±±

9,58±0,1559.58±0.155

20,47±0,45020.47±0.450

0,904±0,0280.904±0.028

0,268±0,0110.268±0.011

9,6489,648

V, %V, %

9,159.15

12,4512.45

17,4117.41

24,5824.58

--

±±

10,18±0,130 ***10.18±0.130 ***

22,71±0,394***22.71±0.394***

1,029±0,024*1.029±0.024*

0,301±0,0120.301±0.012

10,53510,535

V, %V, %

7,537.53

10,2610.26

13,9813.98

24,5224.52

--

IV-Sirop de zahăr pur (martor)IV-Pure sugar syrup (control)

±±

9,06±0,1999.06±0.199

20,07±0,56720.07±0.567

0,884±0,0620.884±0.062

0,257±0,220.257±0.22

3,0843,084

V, %V, %

7,617.61

9,789.78

24,3624.36

29,5129.51

--

[0055] ***Diametrul botcelor: lotul II / lotul IV - ***B1 ≥ 0,999[0055] ***Bottle diameter: batch II / batch IV - ***B1 ≥ 0.999

[0056] ***Lungimea botcelor: lotul III / lotul IV - ***B3 ≥ 0,999[0056] ***Length of the boots: batch III / batch IV - ***B3 ≥ 0.999

[0057] *Masa botcei cu larvă şi lăptişor: lotul III / lotul IV - *B1 ≥ 0,95[0057] *Weight of the boll with larva and milkweed: batch III / batch IV - *B1 ≥ 0.95

[0058] Prin urmare, majorarea numărului de rame (3), a numărului de larve (110 buc.) şi stimularea albinelor doici cu sirop de zahăr în concentraţie de 50% cu biostimulatorul menţionat a sporit acceptarea larvelor la creştere cu 20,9-33,6%, diametrul botcelor cu 5,7-12,3%, lungimea botcelor cu 2,0-13,1%, masa botcelor cu larve şi lăptişor cu 2,3-16,4%, cantitatea lăptişorului obţinut dintr-o botcă cu 4,3-17,1% şi cantitatea totală de lăptişor de matcă cu 6,564-10,734 g faţă de lotul martor.[0058] Therefore, increasing the number of frames (3), the number of larvae (110 pcs.) and stimulating the nurse bees with sugar syrup in a concentration of 50% with the mentioned biostimulator increased the acceptance of larvae to growth by 20.9-33.6%, the diameter of the combs by 5.7-12.3%, the length of the combs by 2.0-13.1%, the mass of the combs with larvae and royal jelly by 2.3-16.4%, the amount of royal jelly obtained from a comb by 4.3-17.1% and the total amount of royal jelly by 6.564-10.734 g compared to the control batch.

[0059] În a treia repriză a fost introdusă câte o ramă cu 40 de larve transvazate. S-a relevat că din numărul total de larve transvazate date la creştere cele mai multe au fost acceptate la creştere în lotul I - 30 buc. sau 75,0% din numărul total. Cu majorarea dozei s-a redus şi procentul larvelor acceptate până la 67,55 (lotul III) (tabelul 5).[0059] In the third half, a frame with 40 transferred larvae was introduced. It was revealed that from the total number of transferred larvae given for growth, most were accepted for growth in batch I - 30 pcs. or 75.0% of the total number. With the increase in the dose, the percentage of accepted larvae was also reduced to 67.55 (batch III) (table 5).

[0060] Tabelul 5[0060] Table 5

[0061] Numărul de larve transvazate şi acceptate la creştere,[0061] Number of larvae transferred and accepted for rearing,

[0062] 20-23.07.2024 (a III-a transvazare, 40 larve)[0062] 20-23.07.2024 (3rd transfer, 40 larvae)

Lotullot

Nr. larvelor transvazate, buc.No. of transferred larvae, pcs.

Nr. larvelor acceptateNo. of larvae accepted

buc.pcs.

%%

I - Sirop de zahăr+ Clorură de colină de 3%, 1,0 ml/lI - Sugar syrup + 3% choline chloride, 1.0 ml/l

4040

3030

75,075.0

4040

2828

70,070.0

4040

2727

67,567.5

IV - Sirop de zahăr pur (martor)IV - Pure sugar syrup (control)

4040

1919

47,547.5

[0064] Diametrul botcelor la a treia zi la loturile experimentale a constituit în medie 9,43-9,92 mm sau cu 0,1-5,3% mai mare faţă de lotul martor, lungimea - 21,34-22,67 mm (5,3-7,6%), masa botcelor cu larve şi lăptişor - 1,02-1,082 g (6,6-13,1%).[0064] The diameter of the bolls on the third day in the experimental groups was on average 9.43-9.92 mm or 0.1-5.3% higher than in the control group, length - 21.34-22.67 mm (5.3-7.6%), mass of bolls with larvae and milkweed - 1.02-1.082 g (6.6-13.1%).

[0065] Cantitatea de lăptişor de matcă obţinut dintr-o botcă a constituit în medie 0,322-0,351 g sau cu 37,0-49,4% mai mult faţă de lotul martor, diferenţa fiind autentică (***B3 ≥ 0,999). Cea mai mare cantitate de lăptişor de matcă s-a obţinut de la lotul II - 9,828 g sau de 2,20 ori mai mult faţă de lotul martor (tabelul 6). Coeficientul de variaţie a indicilor studiaţi au oscilat între 6,96% (diametrul botcelor) şi 42,51% (cantitatea de lăptişor de matcă obţinut dintr-o botcă).[0065] The amount of royal jelly obtained from one beehive was on average 0.322-0.351 g or 37.0-49.4% more than the control batch, the difference being authentic (***B3 ≥ 0.999). The largest amount of royal jelly was obtained from batch II - 9.828 g or 2.20 times more than the control batch (table 6). The coefficient of variation of the studied indices ranged between 6.96% (beehive diameter) and 42.51% (amount of royal jelly obtained from one beehive).

[0066] Tabelul 6[0066] Table 6

[0067] Indicii botcelor şi cantitatea de lăptişor de matcă obţinut,[0067] The indices of the beehives and the amount of royal jelly obtained,

[0068] 20-23.07.2024 (a III-a transvazare, 40 larve)[0068] 20-23.07.2024 (3rd transvasation, 40 larvae)

Lotullot

IndiciiIndices

Diametrul botcelor, mmDiameter of the barrels, mm

Lungimea botcelor, mmLength of the boots, mm

Cantitatea totală de lăptişor obţinut, gTotal amount of milk obtained, g

±±

9,57±0,1389.57±0.138

21,34±0,58021.34±0.580

1,02±0,0421.02±0.042

0,322±0,001**0.322±0.001**

9,6609,660

V, %V, %

7,887.88

14,9014.90

22,7922.79

27,1227.12

--

±±

9,92±0,144*9.92±0.144*

22,67±0,466*22.67±0.466*

1,068±0,0381.068±0.038

0,351±0,015***0.351±0.015***

9,8289,828

V, %V, %

7,697.69

10,8710.87

18,9418.94

22,8522.85

--

±±

9,43±0,1419.43±0.141

22,18±0,48122.18±0.481

1,082±0,0311.082±0.031

0,335±0,011**0.335±0.011**

9,0459,045

V, %V, %

7,457.45

11,2611.26

14,9914.99

16,5516.55

--

IV - Sirop de zahăr pur (martor)IV - Pure sugar syrup (control)

±±

9,42±0,1509.42±0.150

21,06±0,46021.06±0.460

0,957±0,0370.957±0.037

0,235±0,0230.235±0.023

4,4654,465

V, %V, %

6,966.96

9,519.51

16,8216.82

42,5142.51

--

[0070] *Diametrul botcelor: lotul II / lotul IV - *B1 ≥ 0,95[0070] *Bottle diameter: batch II / batch IV - *B1 ≥ 0.95

[0071] *Lungimea botcelor: lotul II / lotul IV - *B1 ≥ 0,95[0071] *Length of the boots: batch II / batch IV - *B1 ≥ 0.95

[0072] ** Cantitatea lăptişorului obţinută dintr-o botcă: lotul I / lotul IV - *B2 ≥ 0,99[0072] ** Quantity of milk obtained from one barrel: batch I / batch IV - *B2 ≥ 0.99

[0073] *** Cantitatea lăptişorului obţinută dintr-o botcă: lotul II / lotul IV - ***B3 ≥ 0,999[0073] *** Quantity of milk obtained from a barrel: batch II / batch IV - ***B3 ≥ 0.999

[0074] ** Cantitatea lăptişorului obţinută dintr-o botcă: lotul III / lotul IV - **B2 ≥ 0,99[0074] ** Quantity of milk obtained from a barrel: batch III / batch IV - **B2 ≥ 0.99

[0075] S-a relevat că cea mai mare cantitate de lăptişor de matcă (9,828 g) s-a obţinut la lotul II căruia i s-a administrat un amestec de sirop de zahăr cu 1,5 ml/L de biostimulator.[0075] It was revealed that the largest amount of royal jelly (9.828 g) was obtained in batch II which was administered a mixture of sugar syrup with 1.5 ml/L of biostimulator.

[0076] Hrănirea albinelor doici în perioada creşterii larvelor pe parcursul a trei zile câte un litru de amestec de sirop de zahăr şi 1,5 ml/l de biostimulator majorează producerea lăptişorului de matcă cu 4,58-5,363 g sau de 2,02-2,20 ori mai mult faţă de lotul martor.[0076] Feeding nurse bees during the larval growth period with one liter of a mixture of sugar syrup and 1.5 ml/l of biostimulator for three days increases royal jelly production by 4.58-5.363 g or 2.02-2.20 times more than the control group.

[0077] În total pe parcursul a trei reprize introducerea în familiile crescătoare a ramelor cu 70, 110 şi 40 larve transvazate şi hrănirea albinelor doici cu un amestec de sirop de zahar şi biostimulator în doză de 1,0-2,0 ml/L a sporit numărul de larve transvazate acceptate la creştere cu 20,9-32,3% şi cantitatea totală de lăptişor de matcă cu 32,695-41,379 g sau de 2,43-3,07 ori mai mult faţă de lotul martor.[0077] In total, over three rounds, the introduction of frames with 70, 110 and 40 transferred larvae into the breeding families and the feeding of nurse bees with a mixture of sugar syrup and biostimulator at a dose of 1.0-2.0 ml/L increased the number of transferred larvae accepted for breeding by 20.9-32.3% and the total amount of royal jelly by 32.695-41.379 g or 2.43-3.07 times more than the control group.

[0078] Tabelul 7[0078] Table 7

[0079] Numărul de larve transvazate şi cantitatea de lăptişor de matcă[0079] Number of transferred larvae and amount of royal jelly

[0080] obţinut pe parcursul a trei reprize (14-17; 17-20 şi 20-23.07.2024)[0080] obtained over three rounds (14-17; 17-20 and 20-23.07.2024)

Lotullot

Numărul de larve transvazate la o repriză/ acceptateNumber of larvae transferred per session/accepted

Numărul total de larve date/ acceptate/%Total number of larvae given/accepted/%

Numărul de larve transvazate la o repriză/ cantitatea de lăptişor obţinut g/%Number of larvae transferred per session/amount of milky fluid obtained g/%

Cantita- tea totală de lăptişor obţinut, gTotal quantity of milk obtained, g

Diferenţa faţă de lotul martorDifference from the control group

7070

110110

4040

7070

110110

4040

gg

%%

5151

4949

3030

220/130/ 59,1220/130/ 59.1

17,901/ 239,417,901/ 239.4

13,818/ 448,613,818/ 448.6

9,66/ 216,39.66/ 216.3

41,37941,379

27,92227,922

307,5307.5

4242

3636

2828

220/106/ 48,2220/106/ 48.2

14,028/ 237,414,028/ 237.4

9,648/ 312,89,648/ 312.8

9,828/ 220,19,828/ 220.1

33,50433,504

20,04720,047

249,0249.0

4343

3535

2727

220/105/47,7220/105/47.7

13,115/ 222,9013,115/ 222,90

10,535/ 341,610,535/ 341.6

9,045/ 202,69.045/ 202.6

32,69532,695

19,26819,268

242,9242.9

IV - Sirop de zahăr pur (martor)IV - Pure sugar syrup (control)

2828

1212

1919

220/59/ 26,8220/59/ 26.8

5,908/ 100,05.908/ 100.0

3,084/ 100,03.084/ 100.0

4,465/ 100,04.465/ 100.0

13,45713,457

--

100,0100.0

[0082] S-a constatat că cea mai mare cantitate de lăptişor de matcă (41,379 g) pe parcursul a trei reprize a fost colectată de la familiile de albine din lotul I.[0082] It was found that the largest amount of royal jelly (41.379 g) over three rounds was collected from bee colonies in batch I.

[0083] Referinţe bibliografice:[0083] Bibliographic references:

[0084] 1. Кривцов Н. И., Лебедев В. И., Туников Г. М. Пчеловодство. Москва, Колос, 2000, р. 192-200[0084] 1. Krivtsov N. I., Lebedev V. I., Tunikov G. M. Beekeeping. Moscow, Kolos, 2000, р. 192-200

[0085] 2. MD 1090 G2 1999.07.31[0085] 2. MD 1090 G2 1999.07.31

[0086] 3. MD 1409 G2 F1 2000.02.29[0086] 3. MD 1409 G2 F1 2000.02.29

[0087] 4. Видео-инструкция. Система Никот, Франция. 23.11.2021, Găsit Internet: https://www.albilux.md/rom/nicot.html[0087] 4. Video instruction. Sistema Nicot, France. 23.11.2021, Found Internet: https://www.albilux.md/rom/nicot.html

[0088] 5. Eremia N. Apicultura. Chişinău, 2020, p. 311, alin. 9[0088] 5. Eremia N. Beekeeping. Chisinau, 2020, p. 311, paragraph 9

[0089] 1. Кривцов Н. И., Лебедев В. И., Туников Г. М. Пчеловодство. Москва, Колос, 2000, р. 192-200[0089] 1. Krivtsov N. I., Lebedev V. I., Tunikov G. M. Beekeeping. Moscow, Kolos, 2000, р. 192-200

[0090] 2. MD 1090 G2 1999.07.31[0090] 2. MD 1090 G2 1999.07.31

[0091] 3. MD 1409 F1 2000.02.29[0091] 3. MD 1409 F1 2000.02.29

[0092] 4. Sistemul Nicot. Видео-инструкция. Система Никот, Франция. 23.11.2021, Găsit Internet < https://www.albilux.md/rom/nicot.html>4. The Nicot System. Video instruction. Sistema Nicot, France. 23.11.2021, Found Internet < https://www.albilux.md/rom/nicot.html>

[0093] 5. Eremia N. Apicultura. Chişinău, 2020, p. 311, alin. 9[0093] 5. Eremia N. Beekeeping. Chisinau, 2020, p. 311, paragraph 9

Claims

1. Royal jelly obtaining process, which includes introducing into the breeding colony 1-3 frames equipped with beehives with 40-110 transferred larvae and daily feeding of the nurse bees, in the absence of a maintenance honey collection, with a mixture of 50% sugar syrup and 1.0-2.0 mL/L of 3% aqueous solution of choline chloride, in an amount of 1.0 L of mixture per bee colony, for 3 days, after which the frames are removed from the hive to collect royal jelly, and the procedure is repeated twice, at the same time standard frames of 435x300 mm are used with 2 slats, at the upper and middle level, equipped with 30 (15/15) beehives, as well as with 3 slats, at the upper, middle and lower level, equipped with 40 (14/13/13) boots, the boots being made up of a fixing cap for the cup holder, the cup holder and the cup.