MD154Y - Process for treating triticale seeds with reduced viability - Google Patents

Process for treating triticale seeds with reduced viability Download PDF

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Publication number
MD154Y
MD154Y MDS20090123A MDS20090123A MD154Y MD 154 Y MD154 Y MD 154Y MD S20090123 A MDS20090123 A MD S20090123A MD S20090123 A MDS20090123 A MD S20090123A MD 154 Y MD154 Y MD 154Y
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Moldova
Prior art keywords
seeds
treating
triticale
reduced viability
viability
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MDS20090123A
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Romanian (ro)
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Liudmila Corlateanu
Anatolie Ganea
Pavel Chintea
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Institutul De Genetica Si Fiziologie A Plantelor Al Academiei De Stiinte A Moldovei
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Priority to MDS20090123A priority Critical patent/MD154Z/en
Publication of MD154Y publication Critical patent/MD154Y/en
Publication of MD154Z publication Critical patent/MD154Z/en

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Abstract

The invention relates to agriculture and may be applied in phytotechny for treating triticale seeds after long-term storage thereof. The process for treating triticale seeds with reduced viability includes their presowing treatment during 18 hours with 0.005...0.05% aqueous solution of tomatoside. The result consists in increasing the triticale seed viability after long-term storage thereof.

Description

Invenţia se referă la agricultură şi poate fi utilizată în fitotehnie pentru prelucrarea seminţelor de triticale după păstrarea lor îndelungată în colecţiile ex situ. The invention relates to agriculture and can be used in plant breeding for the processing of triticale seeds after their long-term storage in ex situ collections.

Condiţiile de păstrare a seminţelor plantelor de cultură în colecţiile ex situ se reglementează prin standarde internaţionale care permit de a păstra germinaţia lor la un nivel înalt în colecţiile de bază şi cele conservate pe termen mediu în Băncile de Gene. În instituţiile de ameliorare, unităţile de cercetare germoplasma se păstrează în colecţii de lucru în condiţii necontrolate de temperatură şi umiditate, cea ce în scurt timp conduce la scăderea şi pierderea viabilităţii lor. În cazul acesta este necesar de a aplica diverse metode în scopul măririi germinaţiei lor. The conditions for storing seeds of cultivated plants in ex situ collections are regulated by international standards that allow to keep their germination at a high level in the basic collections and those preserved for the medium term in Gene Banks. In breeding institutions, germplasm research units are kept in working collections under uncontrolled conditions of temperature and humidity, which in a short time leads to the decrease and loss of their viability. In this case, it is necessary to apply various methods in order to increase their germination.

Este cunoscut procedeul de tratare a seminţelor plantelor de cultură înainte de semănat, în corespundere cu care seminţele se prelucrează cu soluţii de gibberellină [1]. The process of treating crop seeds before sowing is known, in accordance with which the seeds are processed with gibberellin solutions [1].

Dezavantajele procedeului menţionat sunt costul mare al gibberellinei şi necesitatea aplicării unor proceduri speciale. În afară de aceasta, folosirea lor în fitotehnie este asociată cu un şir de dificultăţi - acţiunea orientată asupra diferitelor grupe sistematice ale plantelor şi în cadrul aceleiaşi specii, absenţa efectului stimulator la unele culturi sau inhibarea dezvoltării lor, necesitatea utilizării alcoolului pentru prepararea soluţiilor. The disadvantages of the mentioned process are the high cost of gibberellins and the need to apply special procedures. In addition, their use in phytotechnics is associated with a number of difficulties - the action directed at different systematic groups of plants and within the same species, the absence of a stimulating effect on some crops or inhibition of their development, the need to use alcohol for the preparation of solutions.

Cea mai apropiată soluţie este procedeul de tratare a seminţelor de pătlăgele vinete înainte de semănat, care include înmuierea lor în soluţie apoasă de glicozidă steroidică, şi anume de tomatozidă [2]. De notat, însă, că procedeul propus determină sporirea productivităţii şi a calităţii culturii menţionate prin prelucrarea seminţelor cu germinaţia optimală în condiţii industriale. Este cunoscut faptul că activitatea glicozidelor steroidice, de asemenea, este specifică nu numai la nivelul speciilor botanice, dar şi la cel al soiurilor, hibrizilor, liniilor. Din aceste considerente soluţia menţionată nu poate fi aplicată în mod automat (scopul, concentraţia, expoziţia prelucrării etc.) altei culturi foarte îndepărtate din punct de vedere botanic şi agronomic, cum este triticale (Familia Poaceae). The closest solution is the process of treating eggplant seeds before sowing, which includes soaking them in an aqueous solution of a steroid glycoside, namely tomatoside [2]. It should be noted, however, that the proposed process increases the productivity and quality of the mentioned crop by processing seeds with optimal germination under industrial conditions. It is known that the activity of steroid glycosides is also specific not only at the level of botanical species, but also at the level of varieties, hybrids, lines. For these reasons, the mentioned solution cannot be automatically applied (purpose, concentration, processing exposure, etc.) to another crop that is very distant from a botanical and agronomic point of view, such as triticale (Poaceae Family).

Problema pe care o rezolvă invenţia constă în mărirea viabilităţii seminţelor cu germinaţie redusă după păstrarea lor îndelungată în colecţiile de lucru şi cele active ex situ. The problem solved by the invention consists in increasing the viability of seeds with reduced germination after their long-term storage in working and active ex situ collections.

Problema se soluţionează prin aceea că seminţele de triticale păstrate îndelungat înainte de semănat se tratează cu soluţie apoasă a glicozidei steroidice glucopiranozid (1→2)glucopiranozid (1→4)galactopiranozid-3-[(25R)-5α-3β, 22α, 26-triol]-26-O-β-D-glucopiranozid(tomatozidă) de concentraţia 0,005...0,05% timp de 18 ore. The problem is solved by treating triticale seeds stored for a long time before sowing with an aqueous solution of the steroid glycoside glucopyranoside (1→2)glucopyranoside (1→4)galactopyranoside-3-[(25R)-5α-3β, 22α, 26-triol]-26-O-β-D-glucopyranoside (tomatoside) at a concentration of 0.005...0.05% for 18 hours.

Rezultatul invenţiei constă în sporirea viabilităţii seminţelor de triticale după păstrarea lor îndelungată în colecţiile de lucru şi active ex situ. The result of the invention consists in increasing the viability of triticale seeds after their long-term storage in working and active ex situ collections.

Utilizarea glicozidelor steroidice pentru prelucrarea seminţelor plantelor de cultură este preferabilă, deoarece s-a stabilit mecanismul acţiunii lor, care constă în activizarea diferitelor constituiente membranare intracelulare, fortificarea proceselor metabolice, cea ce conduce la micşorarea acumulării radicalilor liberi, inhibarea vitezei de îmbătrânire a celulelor seminţelor. The use of steroid glycosides for processing crop plant seeds is preferable, because the mechanism of their action has been established, which consists in activating various intracellular membrane constituents, strengthening metabolic processes, which leads to a decrease in the accumulation of free radicals, and inhibiting the aging rate of seed cells.

Exemplu de realizare a invenţiei Example of embodiment of the invention

Seminţele de triticale, soiul KAD 2/917, îmbătrânite în mod artificial se tratează cu soluţie apoasă de tomatozidă de 0,005...0,05% timp de 18 ore la temperatura de 20°C. Pentru obţinerea datelor comparative seminţele se prelucrează şi cu soluţie apoasă de gibberellină (0,001%). Ca martor se folosesc seminţele umectate cu apă distilată. După 18 ore seminţele se scot din soluţii, se spală şi se plasează în cutii Petri pe un strat de hârtie de filtru. Cutiile se introduc în termostat pentru germinare la temperatura de 20°C. În fiecare variantă se utilizează câte 100 de seminţe în 4 repetări. Se determină energia de creştere (la a 4-a zi) şi germinaţia (la a 8-a zi). Triticale seeds, variety KAD 2/917, artificially aged are treated with an aqueous solution of tomatoside of 0.005...0.05% for 18 hours at a temperature of 20°C. To obtain comparative data, the seeds are also processed with an aqueous solution of gibberellin (0.001%). Seeds moistened with distilled water are used as a control. After 18 hours, the seeds are removed from the solutions, washed and placed in Petri dishes on a layer of filter paper. The dishes are placed in a thermostat for germination at a temperature of 20°C. In each variant, 100 seeds are used in 4 repetitions. The growth energy (on the 4th day) and germination (on the 8th day) are determined.

Rezultatele sunt prezentate în tabel. The results are presented in the table.

Tabel Table

Acţiunea tomatozidei asupra seminţelor cu viabilitate redusă din cauza păstrării îndelungate (soiul KAD 2/917) The action of tomatozide on seeds with reduced viability due to long-term storage (KAD 2/917 variety)

Nr. variantei Varianta Concentraţia, % Energia de creştere, % Germinaţia, % 1 Tomatozidă 0,05 35,3±1,2 56,4±2,1 2 „-„ 0,01 41,5±1,9 64,8±2,8 3 „-„ 0,005 30,0±1,3 51,5±1,7 4 „-„ 0,001 25,4±1,1 43,3±1,5 5 Gibberellină 0,001 22,8±1,1 42,5±1,6 6 Martor - 20,3±1,2 40,0±1,3Variant No. Variant Concentration, % Growth energy, % Germination, % 1 Tomatoside 0.05 35.3±1.2 56.4±2.1 2 „-„ 0.01 41.5±1.9 64.8±2.8 3 „-„ 0.005 30.0±1.3 51.5±1.7 4 „-„ 0.001 25.4±1.1 43.3±1.5 5 Gibberellin 0.001 22.8±1.1 42.5±1.6 6 Control - 20.3±1.2 40.0±1.3

După cum se observă din tabel, prelucrarea seminţelor de triticale cu soluţie apoasă de tomatozidă influenţează pozitiv asupra viabilităţii lor, comparativ cu gibberellina. As can be seen from the table, processing triticale seeds with an aqueous solution of tomatoside has a positive influence on their viability, compared to gibberellin.

Aşadar, tratarea prin procedeul propus a seminţelor de triticale cu viabilitate scăzută din cauza păstrării îndelungate contribuie la mărirea energiei lor de creştere şi a germinaţiei. Therefore, treating triticale seeds with low viability due to long storage through the proposed process contributes to increasing their growth energy and germination.

1. Калинин Ф.Л. Биологически активные вещества в растениеводстве (теория и практика применения). Киев, Наукова Думка, 1984, с. 202-204 1. Kalinin F.L. Biologically active substances in horticulture (theory and practice of application). Киев, Наукова Думка, 1984, p. 202-204

2. MD 2156 G2 2003.05.31 2. MD 2156 G2 2003.05.31

Claims (1)

Procedeu de tratare a seminţelor de triticale cu viabilitate redusă care include tratarea seminţelor înainte de semănat cu soluţie apoasă a unei substanţe biologic active, caracterizat prin aceea că tratarea se efectuează cu soluţie apoasă de tomatozidă cu concentraţia de 0,005…0,05%, timp de 18 ore.Process for treating triticale seeds with reduced viability which includes treating the seeds before sowing with an aqueous solution of a biologically active substance, characterized in that the treatment is carried out with an aqueous solution of tomatozide with a concentration of 0.005…0.05%, for 18 hours.
MDS20090123A 2009-07-01 2009-07-01 Process for treating triticale seeds with reduced viability MD154Z (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MD271Z (en) * 2010-06-07 2011-04-30 Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы Process for extraroot treatment of tomato plants
MD791Z (en) * 2013-10-18 2015-02-28 Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы Process for treating common wheat seeds with low viability
MD1041Z (en) * 2015-10-16 2017-01-31 Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы Process for treating triticale seeds with reduced viability
MD1111Z (en) * 2016-08-01 2017-08-31 Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы Process for treating common wheat seeds with reduced viability
MD1237Y (en) * 2017-08-10 2018-03-31 Институт Микробиологии И Биотехнологии Академии Наук Молдовы Process for presowing treatment of cereal seeds

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MD545Z (en) * 2012-04-11 2013-05-31 Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы Process for treating tomato seeds with low viability
MD694Z (en) * 2013-02-28 2014-06-30 Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы Method for assessing the viability of winter wheat after wintering

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MD385C2 (en) * 1994-12-28 1996-06-30 Научно-исследовательский конструкторско-технологический институт табака и табачных изделий Tobacco chemical immunizer to the tobacco tronzing virus
MD2181G2 (en) * 2001-07-24 2003-12-31 Институт Генетики И Физиологии Растений Академии Наук Молдовы Trioside-[(25S)-5a-furostan-3b,22a,26-triol]-26-O-b-D-glucopyranoside, increasing the formation of photosynthetic pigments
MD2156G2 (en) * 2001-07-31 2003-11-30 Институт Генетики И Физиологии Растений Академии Наук Молдовы Proces for egg-plant growing for seeds
MD2688G2 (en) * 2004-07-02 2005-10-31 Центр Растительных Генетических Ресурсов Молдовы Академии Наук Республики Молдова Process for treating cucumber seeds with reduced viability
  • 2009

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MD271Z (en) * 2010-06-07 2011-04-30 Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы Process for extraroot treatment of tomato plants
MD791Z (en) * 2013-10-18 2015-02-28 Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы Process for treating common wheat seeds with low viability
MD1041Z (en) * 2015-10-16 2017-01-31 Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы Process for treating triticale seeds with reduced viability
MD1111Z (en) * 2016-08-01 2017-08-31 Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы Process for treating common wheat seeds with reduced viability
MD1237Y (en) * 2017-08-10 2018-03-31 Институт Микробиологии И Биотехнологии Академии Наук Молдовы Process for presowing treatment of cereal seeds

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