MD545Z - Process for treating tomato seeds with low viability - Google Patents
Process for treating tomato seeds with low viability Download PDFInfo
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- MD545Z MD545Z MDS20120064A MDS20120064A MD545Z MD 545 Z MD545 Z MD 545Z MD S20120064 A MDS20120064 A MD S20120064A MD S20120064 A MDS20120064 A MD S20120064A MD 545 Z MD545 Z MD 545Z
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- tomatozide
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- 235000007688 Lycopersicon esculentum Nutrition 0.000 title claims abstract description 25
- 230000035899 viability Effects 0.000 title claims abstract description 19
- 238000000034 method Methods 0.000 title claims abstract description 15
- 240000003768 Solanum lycopersicum Species 0.000 title claims description 23
- 239000007864 aqueous solution Substances 0.000 claims abstract description 6
- 238000009331 sowing Methods 0.000 claims description 4
- 241000196324 Embryophyta Species 0.000 abstract description 8
- 238000003860 storage Methods 0.000 abstract description 6
- 230000007774 longterm Effects 0.000 abstract description 5
- 241000227653 Lycopersicon Species 0.000 abstract 3
- 230000035784 germination Effects 0.000 description 11
- 229930191978 Gibberellin Natural products 0.000 description 9
- 239000003448 gibberellin Substances 0.000 description 9
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 239000012153 distilled water Substances 0.000 description 6
- 230000012010 growth Effects 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 229930002534 steroid glycoside Natural products 0.000 description 5
- 238000011066 ex-situ storage Methods 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000417 fungicide Substances 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 230000007226 seed germination Effects 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 230000036579 abiotic stress Effects 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229930185631 capsicoside Natural products 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- SIHHLZPXQLFPMC-UHFFFAOYSA-N chloroform;methanol;hydrate Chemical compound O.OC.ClC(Cl)Cl SIHHLZPXQLFPMC-UHFFFAOYSA-N 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 244000038559 crop plants Species 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- CTYOUOHIEXEYAW-MEVSNGMESA-N furostane group Chemical class [C@@H]12C[C@@H]3O[C@H](CCC(C)C)[C@@H](C)[C@@H]3[C@@]1(C)CC[C@H]1[C@H]2CCC2CCCC[C@]12C CTYOUOHIEXEYAW-MEVSNGMESA-N 0.000 description 1
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical class C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 210000004020 intracellular membrane Anatomy 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000000401 methanolic extract Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 239000005648 plant growth regulator Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 241000894007 species Species 0.000 description 1
- INLFWQCRAJUDCR-LYLBMTSKSA-N spirostane group Chemical class [C@@H]12C[C@@H]3O[C@]4(CC[C@@H](C)CO4)[C@@H](C)[C@@H]3[C@@]1(C)CC[C@H]1[C@H]2CCC2CCCC[C@]12C INLFWQCRAJUDCR-LYLBMTSKSA-N 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Landscapes
- Pretreatment Of Seeds And Plants (AREA)
Abstract
Description
Invenţia se referă la agricultură şi poate fi aplicată în fitotehnie pentru tratarea seminţelor de tomate după păstrarea lor îndelungată în colecţiile ex situ. The invention relates to agriculture and can be applied in phytotechnics for treating tomato seeds after their long-term storage in ex situ collections.
Condiţiile de păstrare a seminţelor plantelor în colecţiile ex situ se reglementează prin standarde internaţionale, care permit a păstra germinaţia lor la un nivel înalt în colecţiile de bază şi cele conservate pe termen mediu în Băncile de Gene. În instituţiile de ameliorare şi de cercetare, seminţele se păstrează în colecţii de lucru în condiţii necontrolate de temperatură şi umiditate, ceea ce în scurt timp duce la scăderea şi pierderea viabilităţii lor. În cazul acesta este necesar de a aplica diverse metode în scopul măririi germinaţiei seminţelor. The conditions for storing plant seeds in ex situ collections are regulated by international standards, which allow maintaining their germination at a high level in basic collections and those preserved for the medium term in Gene Banks. In breeding and research institutions, seeds are stored in working collections under uncontrolled temperature and humidity conditions, which in a short time leads to a decrease and loss of their viability. In this case, it is necessary to apply various methods in order to increase seed germination.
Este cunoscut procedeul de tratare a seminţelor plantelor înainte de semănat cu soluţii de giberelină [1]. The process of treating plant seeds before sowing with gibberellin solutions is known [1].
Neajunsul procedeului menţionat constă în costul mare al giberelinei şi necesitatea aplicării unor proceduri speciale. În afară de aceasta, folosirea lor în fitotehnie este asociată cu un şir de dificultăţi - acţiunea orientată asupra diferitor grupe sistematice de plante în cadrul aceleiaşi specii, absenţa efectului stimulator la unele culturi sau inhibarea dezvoltării lor, necesitatea utilizării alcoolului pentru prepararea soluţiilor. The disadvantage of the mentioned procedure is the high cost of gibberellins and the need to apply special procedures. In addition, their use in phytotechnics is associated with a number of difficulties - the targeted action on different systematic groups of plants within the same species, the absence of a stimulating effect on some crops or inhibition of their development, the need to use alcohol for the preparation of solutions.
Este cunoscut procedeul de tratare a seminţelor de tomate cu diferite glicozide steroidice, inclusiv tomatozida şi capsicozida. Este necesar de notat, însă, că investigaţia menţionată ţine de elucidarea consecinţelor acţiunii glicozidelor steroidice asupra seminţelor de tomate exprimate în generaţiile ulterioare prin germinaţia seminţelor, înălţimea plantulelor, rezistenţa la putregaiul cenuşiu, recolta tomatelor etc. În experienţe s-a făcut compararea cu martorul (apa), precum şi cu fungicide. Nu sunt indicate concentraţiile substanţelor, condiţiile de prelucrare. Datele obţinute la studierea acţiunii remanente a substanţelor biologic active asupra diferitor indici cantitativi ai tomatelor nu pot fi luate în considerare la cercetarea fortificării viabilităţii seminţelor îmbătrânite în timpul prezervării lor în Băncile de Gene [2]. The process of treating tomato seeds with various steroid glycosides, including tomatoside and capsicoside, is known. It should be noted, however, that the mentioned investigation is concerned with elucidating the consequences of the action of steroid glycosides on tomato seeds expressed in subsequent generations by seed germination, seedling height, resistance to gray rot, tomato yield, etc. In the experiments, a comparison was made with the control (water), as well as with fungicides. The concentrations of the substances, the processing conditions are not indicated. The data obtained when studying the residual action of biologically active substances on various quantitative indices of tomatoes cannot be taken into account when researching the fortification of the viability of aged seeds during their preservation in Gene Banks [2].
În calitate de cea mai apropiată soluţie poate servi procedeul de tratare a seminţelor de tomate înainte de semănat cu o soluţie apoasă de tomatozidă cu concentraţia de 0,1% timp de 12 ore [3]. The closest solution may be the treatment of tomato seeds before sowing with an aqueous solution of tomatozide at a concentration of 0.1% for 12 hours [3].
Însă autorii acestui procedeu au efectuat investigaţii asupra seminţelor de tomate cu viabilitate bună obţinute de la plantele care au fost prelucrate cu tomatozidă şi apoi cultivate. Seminţele extrase din fructele plantelor tratate au fost păstrate timp de 10 ani. În urma verificării viabilităţii lor după expirarea acestui termen s-a constatat că ea depăşeşte martorul (65…76% comparativ cu 35%). În experienţele efectuate în acest studiu interesele autorilor s-au axat asupra activităţii biologice ale tomatozidei în aspectul sporirii rezistenţei plantelor la diferite boli, cercetării particularităţilor biologice şi agronomice ale plantelor de tomate. De menţionat, însă, că scopurile şi modul de abordare a problemei din partea autorilor procedeului menţionat şi al autorilor procedeului revendicat, sunt diferite. În cea mai apropiata soluţie s-au prelucrat seminţe cu germinaţie bună, pe când în procedeul propus ele aveau o vârstă de 15 ani şi o germinaţie de numai 55,1%. Procedeul propus oferă posibilitatea de păstrare a materialului genetic preţios de tomate deja existent în colecţii active care, din cauza anumitor factori ecologici, şi-a pierdut viabilitatea iniţială. However, the authors of this process conducted investigations on tomato seeds with good viability obtained from plants that were processed with tomatozide and then cultivated. The seeds extracted from the fruits of the treated plants were stored for 10 years. After checking their viability after the expiration of this term, it was found that it exceeds the control (65…76% compared to 35%). In the experiments carried out in this study, the interests of the authors were focused on the biological activity of tomatozide in terms of increasing plant resistance to various diseases, researching the biological and agronomic peculiarities of tomato plants. It should be noted, however, that the goals and the way of approaching the problem on the part of the authors of the mentioned process and the authors of the claimed process are different. In the closest solution, seeds with good germination were processed, while in the proposed process they were 15 years old and had a germination of only 55.1%. The proposed process offers the possibility of preserving the precious tomato genetic material already existing in active collections which, due to certain ecological factors, has lost its initial viability.
Problema pe care o rezolvă invenţia constă în mărirea viabilităţii seminţelor cu germinaţie redusă după păstrarea lor îndelungată în colecţiile de lucru şi cele active ex situ. The problem solved by the invention consists in increasing the viability of seeds with reduced germination after their long-term storage in working and active ex situ collections.
Problema se soluţionează prin aceea că procedeul de tratare a seminţelor de tomate cu viabilitate redusă include tratarea acestora înainte de semănat cu o soluţie apoasă de glicozidă steroidică glucopiranozid (1→2) glucopiranozid (1→4) galactopiranozid-3-[(25R)-5α-3β, 22α, 26-triol]-26-0-β-D-glucopiranozid (tomatozidă) cu concentraţia de 0,01…0,08% timp de 24 ore.The problem is solved by the fact that the process of treating tomato seeds with reduced viability includes treating them before sowing with an aqueous solution of the steroid glycoside glucopyranoside (1→2) glucopyranoside (1→4) galactopyranoside-3-[(25R)-5α-3β, 22α, 26-triol]-26-0-β-D-glucopyranoside (tomatoside) with a concentration of 0.01…0.08% for 24 hours.
Rezultatul invenţiei constă în sporirea viabilităţii seminţelor de tomate după păstrarea lor îndelungată în colecţiile de lucru şi active ex situ. The result of the invention consists in increasing the viability of tomato seeds after their long-term storage in working and active ex situ collections.
Utilizarea glicozidelor steroidice pentru prelucrarea seminţelor plantelor de cultură este preferabilă, deoarece s-a stabilit mecanismul acţiunii lor, care constă în activizarea diferitor constituiente membranare intracelulare, fortificarea proceselor metabolice, ceea ce duce la micşorarea acumulării radicalilor liberi, inhibarea vitezei de îmbătrânire a celulelor seminţelor. The use of steroid glycosides for processing crop plant seeds is preferable, because the mechanism of their action has been established, which consists in activating various intracellular membrane constituents, strengthening metabolic processes, which leads to a decrease in the accumulation of free radicals, and inhibiting the aging rate of seed cells.
Tomatozida se obţine prin extragere din seminţe degresate mărunţite de tomate cu soluţie de 70% de metanol. Extractul metanolic se concentrează prin evaporare până la uscat şi multiplu se cromatografiază pe o coloană cu silicagel. Controlul asupra randamentului preparatului se efectuează prin cromatografierea în strat subţire. Fracţiile de preparat se unesc, apoi se concentrează obţinându-se produsul scontat (temperatura de topire 217…220°C, [α]2° -24°, Rf=0,3, Cloroform-metanol-apă 65/35/10). (Кинтя П.К. и др. Строение и биологическая активность ряда спиростана и фуростана. Кишинев, 1987, с.74-75). Tomatoside is obtained by extraction from crushed defatted tomato seeds with a 70% methanol solution. The methanol extract is concentrated by evaporation to dryness and repeatedly chromatographed on a silica gel column. The yield of the preparation is controlled by thin layer chromatography. The preparation fractions are combined, then concentrated, obtaining the expected product (melting point 217…220°C, [α]2° -24°, Rf=0.3, Chloroform-methanol-water 65/35/10). (Kintya P.K. et al. Structure and biological activity of a series of spirostanes and furostanes. Kishinev, 1987, p.74-75).
Exemplu de realizare a invenţiei Example of embodiment of the invention
Seminţele îmbătrânite de tomate, soiul Victorina (după păstrarea lor timp de 15 ani), se tratează cu soluţie apoasă de tomatozidă cu concentraţia de 0,01…0,1% timp de 6, 12, 18, 24 de ore la temperatura de 25°C (invenţie). Pentru obţinerea datelor comparative seminţele se prelucrează şi cu soluţie apoasă (0,001%) de giberelină. Ca martor se folosesc seminţele umectate cu apă distilată. După 24 de ore seminţele se scot din soluţii, se spală şi se plasează în cutii Petri pe un strat de hârtie de filtru. Cutiile se introduc în termostat pentru germinare la temperatura de 25°C. În fiecare variantă se utilizează câte 100 de seminţe în 4 repetări. Se determină energia de creştere (la a 5-a zi) şi germinaţia (la a 14-a zi). Rezultatele sunt prezentate în tabele. Aged tomato seeds, variety Victorina (after being stored for 15 years), are treated with an aqueous solution of tomatoside with a concentration of 0.01…0.1% for 6, 12, 18, 24 hours at a temperature of 25°C (invention). To obtain comparative data, the seeds are also processed with an aqueous solution (0.001%) of gibberellin. Seeds moistened with distilled water are used as a control. After 24 hours, the seeds are removed from the solutions, washed and placed in Petri dishes on a layer of filter paper. The dishes are placed in a thermostat for germination at a temperature of 25°C. In each variant, 100 seeds are used in 4 repetitions. The growth energy (on the 5th day) and germination (on the 14th day) are determined. The results are presented in tables.
Tabelul 1 Table 1
Acţiunea tomatozidei asupra seminţelor de tomate cu viabilitate redusă The action of tomatozide on tomato seeds with reduced viability
(soiul Victorina, 6 ore) (Victorina variety, 6 hours)
№ Substanţa de tratare Concentraţia, % Energia de creştere, % Germinaţia, % 1. Tomatozidă 0,01 36,2±1,7 54,5±1,2 2. 0,05 38,0±0,9 56,4±3,0 3. 0,08 39,1±1,6* 57,9±2,0* 4. 0,1 35,5±2,1 52,0±1,1 5. Giberelină 0,001 37,0±1,4 57,8±2,5 6. Apă distilată - 32,9±1,2 55,1±1,7№ Treatment substance Concentration, % Growth energy, % Germination, % 1. Tomatoside 0.01 36.2±1.7 54.5±1.2 2. 0.05 38.0±0.9 56.4±3.0 3. 0.08 39.1±1.6* 57.9±2.0* 4. 0.1 35.5±2.1 52.0±1.1 5. Gibberellin 0.001 37.0±1.4 57.8±2.5 6. Distilled water - 32.9±1.2 55.1±1.7
* Diferenţe semnificative faţă de martor la P<0,05 * Significant differences compared to control at P<0.05
Tabelul 2 Table 2
Acţiunea tomatozidei asupra seminţelor de tomate cu viabilitate redusă The action of tomatozide on tomato seeds with reduced viability
(soiul Victorina, 12 ore) (Victorina variety, 12 hours)
№ Substanţa de tratare Concentraţia, % Energia de creştere, % Germinaţia, % 1. Tomatozidă 0,01 35,5±0,6 58,0±1,5 2. 0,05 40,8±1,7 60,9±2,0 3. 0,08 42,5±1,8* 64,0±l,8* 4. 0,1 38,9±1,4 59,0±1,9 5. Giberelină 0,001 37,0±1,4 57,8±2,5 6. Apă distilată - 32,9±1,2 55,1±1,7№ Treatment substance Concentration, % Growth energy, % Germination, % 1. Tomatoside 0.01 35.5±0.6 58.0±1.5 2. 0.05 40.8±1.7 60.9±2.0 3. 0.08 42.5±1.8* 64.0±1.8* 4. 0.1 38.9±1.4 59.0±1.9 5. Gibberellin 0.001 37.0±1.4 57.8±2.5 6. Distilled water - 32.9±1.2 55.1±1.7
* Diferenţe semnificative faţă de martor la P<0,05 * Significant differences compared to control at P<0.05
Tabelul 3 Table 3
Acţiunea tomatozidei asupra seminţelor de tomate cu viabilitate redusă The action of tomatozide on tomato seeds with reduced viability
(soiul Victorina, 18 ore) (Victorina variety, 18 hours)
№ Substanţa de tratare Concentraţia, % Energia de creştere,% Germinaţia, % 1. Tomatozidă 0,01 39,6±2,0 60,9±2,0 2. 0,05 41,9±2,3* 62,8±2,0* 3. 0,08 46,6±1,9* 69,4±1,9* 4. 0,1 38,5±0,9 58,7±1,6 5. Giberelină 0,001 37,0±1,4 57,8±2,5 6. Apă distilată - 32,9±1,2 55,1±1,7№ Treatment substance Concentration, % Growth energy, % Germination, % 1. Tomatoside 0.01 39.6±2.0 60.9±2.0 2. 0.05 41.9±2.3* 62.8±2.0* 3. 0.08 46.6±1.9* 69.4±1.9* 4. 0.1 38.5±0.9 58.7±1.6 5. Gibberellin 0.001 37.0±1.4 57.8±2.5 6. Distilled water - 32.9±1.2 55.1±1.7
* Diferenţe semnificative faţă de martor la P<0,05 * Significant differences compared to control at P<0.05
Tabelul 4 Table 4
Acţiunea tomatozidei asupra seminţelor de tomate cu viabilitate redusă The action of tomatozide on tomato seeds with reduced viability
(soiul Victorina, 24 ore) (Victorina variety, 24 hours)
№ Substanţa de tratare Concentraţia, % Energia de creştere, % Germinaţia, % 1. Tomatozidă 0,01 38,5±2,0 65,4±2,8 2. 0,05 45,9±1,8 68,0±1,7 3. 0,08 54,5±2,1* 79,9±2,6* 4. 0,1 40.9±1,9 61,5±2,7 5. Giberelină 0,001 37,0±1,4 57,8±2,5 6. Apă distilată - 32,9±1,2 55,1±1,7№ Treatment substance Concentration, % Growth energy, % Germination, % 1. Tomatoside 0.01 38.5±2.0 65.4±2.8 2. 0.05 45.9±1.8 68.0±1.7 3. 0.08 54.5±2.1* 79.9±2.6* 4. 0.1 40.9±1.9 61.5±2.7 5. Gibberellin 0.001 37.0±1.4 57.8±2.5 6. Distilled water - 32.9±1.2 55.1±1.7
* Diferenţe semnificative faţă de martor la P<0,05 * Significant differences compared to control at P<0.05
Din tabele se observă că efectul maximal de la prelucrarea cu tomatozidă se atestă la o concentraţie de 0,08% la expoziţia de 24 de ore. From the tables it can be seen that the maximum effect from tomatozide processing is attested at a concentration of 0.08% at 24-hour exposure.
Tabelul 5 Table 5
Acţiunea tomatozidei asupra seminţelor de tomate cu viabilitate redusă The action of tomatozide on tomato seeds with reduced viability
(soiul Victorina) (Victorina variety)
№ Substanţa de tratare Concentraţia, % Energia de creştere, % Germinaţia, % Lungimea rădăciniţelor, mm 1. Tomatozidă 0,01 38,5±2.0 65,4±2,8 29,3±1,8* 2. 0,05 45,9±1,8 68,0±1,7 30,8±2,4* 3. 0,08 54,5±2,1 79,9±2,6 32,6±2,1* 4. 0,1 40,9±1,9 61,5±2,7 26,7±2,9 5. Giberelină 0,001 37,0±1,4 57,8±2,5 26,2±2,1 6. Apă distilată - 32,9 ±1,2 55,1±1,7 24,7±1,9№ Treatment substance Concentration, % Growth energy, % Germination, % Root length, mm 1. Tomatoside 0.01 38.5±2.0 65.4±2.8 29.3±1.8* 2. 0.05 45.9±1.8 68.0±1.7 30.8±2.4* 3. 0.08 54.5±2.1 79.9±2.6 32.6±2.1* 4. 0.1 40.9±1.9 61.5±2.7 26.7±2.9 5. Gibberellin 0.001 37.0±1.4 57.8±2.5 26.2±2.1 6. Distilled water - 32.9 ±1.2 55.1±1.7 24.7±1.9
* Diferenţe semnificative faţă de martor la P<0,95 * Significant differences from control at P<0.95
Lungimea rădăciniţelor în varianta optimă a fost superioară martorului cu 7,9 mm şi cu 6,4 mm mai mare faţă de tratarea cu giberelină. The length of the roots in the optimal variant was superior to the control by 7.9 mm and 6.4 mm greater than the gibberellin treatment.
Aşadar, tratarea prin procedeul propus a seminţelor de tomate cu viabilitate scăzută din cauza păstrării îndelungate contribuie la sporirea viabilităţii acestora. Therefore, treating tomato seeds with low viability due to long storage through the proposed process contributes to increasing their viability.
1. Салей Л.А. Применение регуляторов роста в растениеводстве. Справочник. Кишинев, Штиинца, 1981, с.56-57 1. Салей Л.А. Application of plant growth regulators. Reference book. Chişinău, Shtiintsa, 1981, p.56-57
2. Поликсенова В.Д., Лапунова Т.Н., Стадниченко М.А. Стероидные гликозиды и системные фунгициды как индукторы устойчивости пролонгированного действия на культуре томата. Овощеводство. Сборник научных трудов, том 18, Минск, 2010, с.135-142 2. Poliksenova V.D., Lapunova T.N., Stadnichenko M.A. Steroid glycosides and systemic fungicides as inducers of resistance of prolonged action on tomato culture. Vegetables. Сборник научных трудов, volume 18, Minsk, 2010, pp. 135-142
3. Поликсенова В. Д. Индуцированная устойчивость растений к патогенам и абиотическим стрессовым факторам (на примере томата). Вестник БГУ, № 1, сер. 2, 2009, с. 48-52 3. Поликсенова В. D. Induced resistance of plants to pathogens and abiotic stress factors (eg tomato). Vestnik BGU, № 1, ser. 2, 2009, p. 48-52
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD694Z (en) * | 2013-02-28 | 2014-06-30 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Method for assessing the viability of winter wheat after wintering |
| MD702Z (en) * | 2013-07-26 | 2014-07-31 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Process for presowing treatment of tomato seeds |
| MD791Z (en) * | 2013-10-18 | 2015-02-28 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Process for treating common wheat seeds with low viability |
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| MD2467G2 (en) * | 2003-07-25 | 2005-01-31 | Институт Физиологии Растений Академии Наук Республики Молдова | Process for sugar beet cultivation |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| MD2467G2 (en) * | 2003-07-25 | 2005-01-31 | Институт Физиологии Растений Академии Наук Республики Молдова | Process for sugar beet cultivation |
| MD154Z (en) * | 2009-07-01 | 2010-10-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Process for treating triticale seeds with reduced viability |
| MD153Z (en) * | 2009-07-01 | 2010-10-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Process for treating chick pea seeds with reduced viability |
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Non-Patent Citations (4)
| Title |
|---|
| Поликсенова В. Д. Индуцированная устойчивость растений к патогенам и абиотическим стрессовым факторам (на примере томата). Вестник БГУ, № 1, сер. 2, 2009, с. 48-52 * |
| Поликсенова В.Д., Лапунова Т.Н., Стадниченко М.А. Стероидные гликозиды и системные фунгициды как индукторы устойчивости пролонгированного действия на культуре томата. Овощеводство. Сборник научных трудов, том 18, Минск, 2010, с.135-142 * |
| Салей Л.А. Применение регуляторов роста в растениеводстве. Справочник. Кишинев, Штиинца, 1981, с.56-57 * |
| Сыромятников Ю. Котенко Е., Швец С., Кинтя П. Влияние новых стероидных гликозидов на рефкцию сортов томатов. Материалы 1Х междцнародного симпозиума 2011.06.14-18, Москва, 2011 с.159-162 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD694Z (en) * | 2013-02-28 | 2014-06-30 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Method for assessing the viability of winter wheat after wintering |
| MD702Z (en) * | 2013-07-26 | 2014-07-31 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Process for presowing treatment of tomato seeds |
| MD791Z (en) * | 2013-10-18 | 2015-02-28 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Process for treating common wheat seeds with low viability |
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| MD545Y (en) | 2012-10-31 |
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