MD702Z - Process for presowing treatment of tomato seeds - Google Patents

Process for presowing treatment of tomato seeds Download PDF

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MD702Z
MD702Z MDS20130135A MDS20130135A MD702Z MD 702 Z MD702 Z MD 702Z MD S20130135 A MDS20130135 A MD S20130135A MD S20130135 A MDS20130135 A MD S20130135A MD 702 Z MD702 Z MD 702Z
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Moldova
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auxin
enoxil
tomato
seeds
growth
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MDS20130135A
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Romanian (ro)
Russian (ru)
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Галина ЛУПАШКУ
София ГРИГОРЧА
Лучиан ЛУПАШКУ
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Институт Генетики И Физиологии Растений Академии Наук Молдовы
Институт Химии Академии Наук Молдовы
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Priority to MDS20130135A priority Critical patent/MD702Z/en
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Abstract

Invenţia se referă la agricultură şi poate fi utilizată pentru tratarea seminţelor de tomate înainte de semănat pentru stimularea germinaţiei lor şi a creşterii rădăcinilor plantelor în condiţii de infecţii fungice.Procedeul de tratare a seminţelor înainte de semănat constă în aceea că seminţele se înmoaie în soluţie apoasă de enotaninuri hidrosolubile cu concentraţia de 5·10-5…5·10-3%, timp de 4 ore, după care seminţele se usucă la temperatura de 22°C timp de 24 ore, totodată se utilizează enotaninuri hidrosolubile obţinute la tratarea enotaninurilor cu peroxid de hidrogen în raport masic de 1:(3…6), timp de 7…15 min, la temperatura de 70…100° C.The invention relates to agriculture and can be used to treat tomato seeds before sowing to stimulate their germination and growth of plant roots under conditions of fungal infections. of water-soluble enotanins with a concentration of 5 · 10–5… 5 · 10–3% for 4 hours, after which the seeds are dried at 22 ° C for 24 hours, at the same time water-soluble enotanins obtained when treating enotanins with hydrogen peroxide with a mass ratio of 1: (3 ... 6), for 7 ... 15 min, at a temperature of 70 ... 100 ° C.

Description

Invenţia se referă la agricultură şi poate fi utilizată pentru tratarea seminţelor de tomate înainte de semănat pentru stimularea germinaţiei lor şi a creşterii rădăcinilor plantelor în condiţii de infecţii fungice. The invention relates to agriculture and can be used for treating tomato seeds before sowing to stimulate their germination and plant root growth under conditions of fungal infections.

În calitate de cea mai apropiată soluţie este cunoscut procedeul de tratare a seminţelor de tomate cu o substanţă biologic activă, care reprezintă o soluţie apoasă de Auxină cu capacitatea de stimulare a creşterii şi dezvoltării rădăcinilor în cazul infectării lor cu fungi [1]. As the closest solution, the process of treating tomato seeds with a biologically active substance, which is an aqueous solution of Auxin with the ability to stimulate the growth and development of roots in case of fungal infection, is known [1].

Dezavantajul acestei soluţii constă în faptul că substanţa activă reprezintă un produs sintetic şi nu îşi manifestă pe deplin potenţialul de stimulare a germinaţiei seminţelor şi a creşterii sistemului radicular al plantelor. The disadvantage of this solution is that the active substance is a synthetic product and does not fully manifest its potential to stimulate seed germination and plant root system growth.

Problema pe care o rezolvă invenţia solicitată constă în extinderea asortimentului de substanţe biologic active de origine naturală cu efect sporit asupra germinaţiei seminţelor şi creşterii sistemului radicular al plantelor de tomate în cazul infectării lor cu fungi. The problem solved by the claimed invention consists in expanding the assortment of biologically active substances of natural origin with an enhanced effect on seed germination and root system growth of tomato plants in case of their infection with fungi.

Procedeul de tratare a seminţelor înainte de semănat constă în aceea că seminţele se înmoaie în soluţie apoasă de enotaninuri hidrosolubile cu concentraţia de 5·10-5…5·10-3% (Enoxil), timp de 4 ore, după care seminţele se usucă la temperatura de 22°C timp de 24 ore, totodată se utilizează enotaninuri hidrosolubile obţinute la tratarea enotaninurilor cu peroxid de hidrogen în raport masic de 1:(3…6), timp de 7…15 min, la temperatura de 70…100° C. The seed treatment process before sowing consists of soaking the seeds in an aqueous solution of water-soluble enotannins with a concentration of 5·10-5…5·10-3% (Enoxil), for 4 hours, after which the seeds are dried at a temperature of 22°C for 24 hours, while using water-soluble enotannins obtained by treating enotannins with hydrogen peroxide in a mass ratio of 1:(3…6), for 7…15 min, at a temperature of 70…100° C.

Enotaninurile hidrosolubile reprezintă un complex de grupări polare acide, peroxidice, alcoolice, fenolice, aldehidice, cetonice, esterice (MD 3125 G2 2007.06.30). Water-soluble enotannins represent a complex of polar acidic, peroxidic, alcoholic, phenolic, aldehyde, ketone, ester groups (MD 3125 G2 2007.06.30).

Rezultatul tehnic al invenţiei constă în aceea că remediul Enoxil, în funcţie de concentraţie - 5·10-5 … 5·10-3%, contribuie la sporirea germinaţiei şi a creşterii lungimii rădăcinilor plantelor de tomate cu 13,3…18,3% şi, respectiv, 9,1…39,5%, în raport cu cea mai apropiată soluţie şi cu 15,0…36,7 % şi, respectiv, 15,6…86,8%, în raport cu varianta martor - filtrat de cultură Alternaria alternata (Fr.) Keissler. The technical result of the invention consists in the fact that the Enoxil remedy, depending on the concentration - 5·10-5 ... 5·10-3%, contributes to increasing the germination and growth of the length of tomato plant roots by 13.3...18.3% and, respectively, 9.1...39.5%, in relation to the closest solution and by 15.0...36.7% and, respectively, 15.6...86.8%, in relation to the control variant - Alternaria alternata (Fr.) Keissler culture filtrate.

Exemplu de realizare a invenţiei Example of embodiment of the invention

Testările s-au efectuat utilizând seminţe hibride de tomate care necesită o protecţie mai riguroasă împotriva fitopatogenului A. alternata care atacă sistemul radicular al plantulelor la etapa timpurie de creştere şi dezvoltare. Materialul semincer de tomate s-a obţinut prin încrucişarea reciprocă a soiurilor Mihaela şi Maestro, florile fiind castrate manual. The tests were carried out using hybrid tomato seeds that require more rigorous protection against the phytopathogen A. alternata that attacks the root system of seedlings at the early stage of growth and development. The tomato seed material was obtained by mutual crossing of the Mihaela and Maestro varieties, the flowers being castrated manually.

Tulpina de fungi A. alternata a fost izolată din rădăcini de tomate cu semne de boală (pete brunificate) pe mediu nutritiv solid Potato Dextrose Agar (PDA) şi identificată prin analize macro- şi microscopice. Filtratul de cultură (FC) al ciupercii A.alternata a fost obţinut prin inocularea miceliului în mediul lichid Czapek-Dox şi cultivat ulterior la temperatura de 22…24ºC, timp de 21 zile. The fungal strain A. alternata was isolated from tomato roots with signs of disease (brown spots) on solid nutrient medium Potato Dextrose Agar (PDA) and identified by macro- and microscopic analyses. The culture filtrate (CF) of the fungus A. alternata was obtained by inoculating the mycelium in liquid Czapek-Dox medium and subsequently cultivated at a temperature of 22…24ºC for 21 days.

Seminţele de tomate au fost tratate timp de 4 ore conform următoarelor variante: apă distilată (martor absolut), FC A.alternata (martor), Auxină în concentraţiile de 5·10-3, 5·10-4, 5·10-5% (cea mai apropiată soluţie), Enoxil în concentraţiile de 5·10-3, 5·10-4, 5·10-5% (invenţia propusă), apoi seminţele au fost uscate la temperatura de 22°C timp de 24 ore. După aceasta, seminţele au fost tratate timp de 18 ore cu FC (cu excepţia variantei martor absolut), după clătire, de 3 ori cu apă distilată, au fost plasate în cutii Petri, între 2 foiţe de hârtie de filtru umectate cu apă distilată, şi menţinute în termostat la temperatura de 24…25°C, timp de 6 zile. Reacţia plantelor la tratament a fost stabilită în baza unor importanţi indici de creştere şi dezvoltare a plantulelor de tomate la etapa timpurie a ontogenezei - germinaţia şi lungimea rădăcinilor (tab.1…4). Tomato seeds were treated for 4 hours according to the following variants: distilled water (absolute control), FC A.alternata (control), Auxin in concentrations of 5·10-3, 5·10-4, 5·10-5% (the closest solution), Enoxil in concentrations of 5·10-3, 5·10-4, 5·10-5% (the proposed invention), then the seeds were dried at a temperature of 22°C for 24 hours. After this, the seeds were treated for 18 hours with FC (except for the absolute control variant), after rinsing, 3 times with distilled water, they were placed in Petri dishes, between 2 sheets of filter paper moistened with distilled water, and kept in a thermostat at a temperature of 24…25°C, for 6 days. The reaction of plants to the treatment was established based on important growth and development indices of tomato seedlings at the early stage of ontogenesis - germination and root length (tab. 1...4).

Tabelul 1 Table 1

Date comparative cu privire la acţiunea Auxinei şi Enoxilului asupra unor indici de creştere la soiul de tomate Mihaela, pe fundal cu filtrat de cultură A.alternata Comparative data on the action of Auxin and Enoxil on some growth indices of the Mihaela tomato variety, on a background with A. alternata culture filtrate

Nr. Variantă Germinaţia Lungimea rădăcinilor Gene- rală, % Diferenţa de “FC”, % Diferenţa „Enoxil - Auxină”, % x±mx, mm Raport faţă de “FC”, % Raport „Enoxil/ Auxină”, % 1 Martor (H2O) 100,0 +36,7 - 37,82±1,07* 146,0 - 2 FC 63,3 - - 25,90±0,44 - - 3 Auxină - 5·10-3% + FC 80,0 +16,7 - 41,00±0,65* 158,3 - 4 Auxină - 5·10-4% + FC 75,0 +11,7 - 34,27±1,02* 132,3 - 5 Auxină - 5·10-5% + FC 73,3 +10,0 - 27,82±1,23 107,4 - 6 Enoxil - 5·10-3% + FC 93,3 +30,0 13,3 48,38±1,36* 186,8 118,0 7 Enoxil - 5·10-4% + FC 90,0 +26,7 15,0 39,56±0,73* 152,7 115,4 8 Enoxil - 5·10-5% + FC 86,7 +23,4 13,4 34,65±1,05* 133,8 124,5No. Variant Germination Root length General, % Difference from “FC”, % Difference “Enoxil - Auxin”, % x±mx, mm Ratio to “FC”, % Ratio “Enoxil/ Auxin”, % 1 Control (H2O) 100.0 +36.7 - 37.82±1.07* 146.0 - 2 FC 63.3 - - 25.90±0.44 - - 3 Auxin - 5·10-3% + FC 80.0 +16.7 - 41.00±0.65* 158.3 - 4 Auxin - 5·10-4% + FC 75.0 +11.7 - 34.27±1.02* 132.3 - 5 Auxin - 5·10-5% + FC 73.3 +10.0 - 27.82±1.23 107.4 - 6 Enoxyl - 5·10-3% + FC 93.3 +30.0 13.3 48.38±1.36* 186.8 118.0 7 Enoxyl - 5·10-4% + FC 90.0 +26.7 15.0 39.56±0.73* 152.7 115.4 8 Enoxyl - 5·10-5% + FC 86.7 +23.4 13.4 34.65±1.05* 133.8 124.5

*- deosebire veridică de varianta FC la nivelul p≤0,05. *- true difference from the FC variant at the p≤0.05 level.

Conform datelor din tab.1, pentru soiul de tomate Mihaela, utilizarea preparatului Enoxil în raport cu cea mai apropiată soluţie (Auxină) a contribuit la sporirea germinaţiei seminţelor şi a creşterii lungimii rădăcinilor cu 13,3…15,0 şi, respectiv, 15,4… 24,5%. According to the data in Table 1, for the Mihaela tomato variety, the use of the Enoxil preparation in relation to the closest solution (Auxin) contributed to increasing seed germination and increasing root length by 13.3…15.0 and, respectively, 15.4…24.5%.

Tabelul 2 Table 2

Date comparative cu privire la acţiunea Auxinei şi Enoxilului asupra unor indici de creştere la soiul de tomate Maestro, pe fundal cu filtrat de cultură A.alternata Comparative data on the action of Auxin and Enoxil on some growth indices of the Maestro tomato variety, on a background with A. alternata culture filtrate

Nr. Variantă Germinaţia Lungimea rădăcinilor Gene- rală, % Diferenţa de “FC”, % Diferenţa „Enoxil - Auxină”, % x±mx, mm Raport faţă de “FC”, % Raport „Enoxil/ Auxină”, % 1 Martor (H2O) 96,7 +43,4 - 35,47±1,32* 148,5 - 2 FC 53,3 - - 23,88±0,92 - - 3 Auxină - 5·10-3% + FC 76,7 +23,4 - 29,65±0,84* 124,2 - 4 Auxină - 5·10-4% + FC 71,7 +18,4 - 27,11±0,87* 113,5 - 5 Auxină - 5·10-5% + FC 70,0 +16,7 - 24,38±0,95 102,1 - 6 Enoxil - 5·10-3% + FC 90,0 +36,7 13,3 39,56±1,02* 165,7 133,4 7 Enoxil - 5·10-4% + FC 86,7 +33,4 15,0 36,33±0,79* 152,3 134,0 8 Enoxil - 5·10-5% + FC 83,3 +30,0 13,3 34,02±0,74* 142,5 139,5No. Variant Germination Root length General, % Difference from “FC”, % Difference “Enoxil - Auxin”, % x±mx, mm Ratio to “FC”, % Ratio “Enoxil/ Auxin”, % 1 Control (H2O) 96.7 +43.4 - 35.47±1.32* 148.5 - 2 FC 53.3 - - 23.88±0.92 - - 3 Auxin - 5·10-3% + FC 76.7 +23.4 - 29.65±0.84* 124.2 - 4 Auxin - 5·10-4% + FC 71.7 +18.4 - 27.11±0.87* 113.5 - 5 Auxin - 5·10-5% + FC 70.0 +16.7 - 24.38±0.95 102.1 - 6 Enoxyl - 5·10-3% + FC 90.0 +36.7 13.3 39.56±1.02* 165.7 133.4 7 Enoxyl - 5·10-4% + FC 86.7 +33.4 15.0 36.33±0.79* 152.3 134.0 8 Enoxyl - 5·10-5% + FC 83.3 +30.0 13.3 34.02±0.74* 142.5 139.5

*- deosebire veridică de varianta FC la nivelul p≤0,05. *- true difference from the FC variant at the p≤0.05 level.

Conform datelor din tab. 2, pentru soiul de tomate Maestro, utilizarea preparatului Enoxil în raport cu cea mai apropiată soluţie (Auxină) a contribuit la sporirea germinaţiei seminţelor şi a creşterii lungimii rădăcinilor cu 13,3…15,0 şi, respectiv, 33,4…39,5%. According to the data in Table 2, for the Maestro tomato variety, the use of the Enoxil preparation in relation to the closest solution (Auxin) contributed to increasing seed germination and increasing root length by 13.3…15.0 and, respectively, 33.4…39.5%.

Tabelul 3 Table 3

Date comparative cu privire la acţiunea Auxinei şi Enoxilului asupra unor indici de creştere la hibridul de tomate F1 Mihaela x Maestro, pe fundal cu filtrat de cultură A.alternata Comparative data on the action of Auxin and Enoxil on some growth indices of the F1 Mihaela x Maestro tomato hybrid, on a background with A. alternata culture filtrate

Nr. Variantă Germinaţia Lungimea rădăcinilor Gene- rală, % Diferenţa de “FC”, % Diferenţa „Enoxil - Auxină”, % x±mx, mm Raport faţă de “FC”, % Raport „Enoxil/ Auxină”, % 1 Martor (H2O) 100,0 +25,0 - 45,87±1,01* 144,7 - 2 FC 75,0 - - 31,71±0,60 - - 3 Auxină- 5·10-3% + FC 81,7 +6,7 - 37,98±0,90* 119,8 - 4 Auxină - 5·10-4% + FC 78,3 +3,3 - 35,43±0,74* 111,7 - 5 Auxină - 5·10-5% + FC 75,0 0,0 - 32,20±1,01 101,5 - 6 Enoxil - 5·10-3% + FC 100,0 +25,0 18,3 43,12±0,67* 135,9 113,5 7 Enoxil - 5·10-4% + FC 95,0 +20,0 16,7 39,04±0,76* 123,1 110,2 8 Enoxil - 5·10-5% + FC 90,0 +15,0 15,0 36,65±0,86* 115,6 113,8No. Variant Germination Root length General, % Difference from “FC”, % Difference from “Enoxil - Auxin”, % x±mx, mm Ratio to “FC”, % Ratio “Enoxil/ Auxin”, % 1 Control (H2O) 100.0 +25.0 - 45.87±1.01* 144.7 - 2 FC 75.0 - - 31.71±0.60 - - 3 Auxin- 5·10-3% + FC 81.7 +6.7 - 37.98±0.90* 119.8 - 4 Auxin - 5·10-4% + FC 78.3 +3.3 - 35.43±0.74* 111.7 - 5 Auxin - 5·10-5% + FC 75.0 0.0 - 32.20±1.01 101.5 - 6 Enoxyl - 5·10-3% + FC 100.0 +25.0 18.3 43.12±0.67* 135.9 113.5 7 Enoxyl - 5·10-4% + FC 95.0 +20.0 16.7 39.04±0.76* 123.1 110.2 8 Enoxyl - 5·10-5% + FC 90.0 +15.0 15.0 36.65±0.86* 115.6 113.8

*- deosebire veridică de varianta FC la nivelul p≤0,05. *- true difference from the FC variant at the p≤0.05 level.

Conform datelor din tab. 3, pentru hibridul de tomate F1 Mihaela x Maestro, utilizarea preparatului Enoxil în raport cu cea mai apropiată soluţie (Auxină) a contribuit la sporirea germinaţiei seminţelor şi a creşterii lungimii rădăcinilor cu 15,0…18,3 şi, respectiv, 13,5…13,8%. According to the data in Table 3, for the tomato hybrid F1 Mihaela x Maestro, the use of the Enoxil preparation in relation to the closest solution (Auxin) contributed to increasing seed germination and increasing root length by 15.0…18.3 and, respectively, 13.5…13.8%.

Tabelul 4 Table 4

Date comparative cu privire la acţiunea Auxinei şi Enoxilului asupra unor indici de creştere la hibridul de tomate F1 Maestro x Mihaela, pe fundal cu filtrat de cultură A.alternata Comparative data on the action of Auxin and Enoxil on some growth indices of the tomato hybrid F1 Maestro x Mihaela, on a background with A. alternata culture filtrate

Nr. Variantă Germinaţia Lungimea rădăcinilor Gene- rală, % Diferenţa de “FC”, % Diferenţa „Enoxil - Auxină”, % x±mx, mm Raport faţă de “FC”, % Raport „Enoxil/ Auxină”, % 1 Martor (H2O) 90,0 +36,7 - 33,54±0,96* 131,9 - 2 FC 53,3 - - 25,41±0,34 - - 3 Auxină- 5·10-3% + FC 70,0 +16,7 - 34,88±0,80* 137,3 - 4 Auxină - 5·10-4% + FC 66,7 +13,4 - 32,98±0,83* 129,8 - 5 Auxină - 5·10-5% + FC 65,0 +11,7 - 30,21±0,62* 118,9 - 6 Enoxil - 5·10-3% + FC 86,7 +33,4 16,7 38,54±0,85* 151,7 110,5 7 Enoxil - 5·10-4% + FC 83,3 +30,0 16,6 36,00±0,95* 141,7 109,1 8 Enoxil - 5·10-5% + FC 80,0 +26,7 15,0 34,23±0,49* 134,7 113,3No. Variant Germination Root length General, % Difference from “FC”, % Difference from “Enoxil - Auxin”, % x±mx, mm Ratio to “FC”, % Ratio “Enoxil/ Auxin”, % 1 Control (H2O) 90.0 +36.7 - 33.54±0.96* 131.9 - 2 FC 53.3 - - 25.41±0.34 - - 3 Auxin- 5·10-3% + FC 70.0 +16.7 - 34.88±0.80* 137.3 - 4 Auxin - 5·10-4% + FC 66.7 +13.4 - 32.98±0.83* 129.8 - 5 Auxin - 5·10-5% + FC 65.0 +11.7 - 30.21±0.62* 118.9 - 6 Enoxyl - 5·10-3% + FC 86.7 +33.4 16.7 38.54±0.85* 151.7 110.5 7 Enoxyl - 5·10-4% + FC 83.3 +30.0 16.6 36.00±0.95* 141.7 109.1 8 Enoxyl - 5·10-5% + FC 80.0 +26.7 15.0 34.23±0.49* 134.7 113.3

*- deosebire veridică de varianta FC la nivelul p≤0,05. *- true difference from the FC variant at the p≤0.05 level.

Conform datelor din tab. 4, pentru hibridul de tomate F1 Maestro x Mihaela, utilizarea preparatului Enoxil în raport cu cea mai apropiată soluţie (Auxină) a contribuit la sporirea germinaţiei seminţelor şi a creşterii lungimii rădăcinilor cu 15,0…16,7 şi, respectiv, 9,1… 13,3%. According to the data in Table 4, for the tomato hybrid F1 Maestro x Mihaela, the use of the Enoxil preparation in relation to the closest solution (Auxin) contributed to increasing seed germination and increasing root length by 15.0…16.7 and, respectively, 9.1…13.3%.

După cum se vede din datele prezentate, tratarea seminţelor cu Enoxil în concentraţiile de 5·10-5 …5·10-3% contribuie la sporirea nivelului de germinaţie şi de creştere a lungimii rădăcinilor în raport cu cea mai apropiată soluţie pentru ambele soiuri şi hibrizi de tomate. As can be seen from the presented data, seed treatment with Enoxil in concentrations of 5·10-5 …5·10-3% contributes to increasing the level of germination and growth of root length compared to the closest solution for both tomato varieties and hybrids.

Prin urmare, acest procedeu este tehnologic simplu, eficient şi permite utilizarea substanţei naturale Enoxil pentru stimularea creşterii plantelor de tomate în cazul infecţiilor fungice care afectează germinaţia, creşterea şi dezvoltarea rădăcinii. Therefore, this process is technologically simple, efficient and allows the use of the natural substance Enoxil to stimulate the growth of tomato plants in the case of fungal infections that affect germination, growth and root development.

1. US 20050043177 A1 2005.02.24 1. US 20050043177 A1 2005.02.24

Claims (1)

Procedeu de tratare a seminţelor de tomate înainte de semănat, care constă în aceea că seminţele se înmoaie în soluţie apoasă de enotaninuri hidrosolubile cu concentraţia de 5·10-5…5·10-3%, timp de 4 ore, după care seminţele se usucă la temperatura de 22°C timp de 24 ore, totodată se utilizează enotaninuri hidrosolubile obţinute la tratarea enotaninurilor cu peroxid de hidrogen în raport masic de 1:(3…6), timp de 7…15 min, la temperatura de 70…100° C.Process for treating tomato seeds before sowing, which consists in soaking the seeds in an aqueous solution of water-soluble enotannins with a concentration of 5·10-5…5·10-3%, for 4 hours, after which the seeds are dried at a temperature of 22°C for 24 hours, at the same time using water-soluble enotannins obtained by treating enotannins with hydrogen peroxide in a mass ratio of 1:(3…6), for 7…15 min, at a temperature of 70…100° C.
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MD2620G2 (en) * 2003-12-16 2005-07-31 Государственный Университет Молд0 Process for presowing tretament of tomato seeds
MD545Z (en) * 2012-04-11 2013-05-31 Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы Process for treating tomato seeds with low viability
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MD573G2 (en) * 1995-10-11 1997-03-31 Государственный Университет Молд0 Method of tomatoes seeds treatment before sowing
US20050043177A1 (en) * 2003-08-22 2005-02-24 Stoller Jerry H. Controlling plant pathogens and pests with applied or induced auxins
MD2620G2 (en) * 2003-12-16 2005-07-31 Государственный Университет Молд0 Process for presowing tretament of tomato seeds
MD545Z (en) * 2012-04-11 2013-05-31 Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы Process for treating tomato seeds with low viability

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